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김미희,송희선,조경우,이상귀,최훈,김선희,김경숙,설경환 대한마취과학회 1989 Korean Journal of Anesthesiology Vol.22 No.1
To investingate the effects of fentanyl on the renal function and the renin secretion rate(RSR), the author measured the urine volume(UV), excreted amount of urinary sodium, potassium and chloride(UNaV, UKV, and UCLV), fractional excretion of sodium(FENa), renal plasma flow(RPF), glomerular filtration rate(GFR), filtration fraction(FF) and free water clearance(CH₂O) after adminis- tration of fentanyl in 53 unanesthetized white rabbits. Fentanyl was given intrarenally(0.1μg/kg/min in group I, 0.3μg/kg/min in group II, and 1.0μg/kg/ min in group III), or intravenously(0.1μg/kg/min in group IV, 1.0μg/kg/min in group V, and 0.1μg/ kg/min in after preinfusion with naloxone 0.1μg/kg/min in group VI). After administration of fentanyl, urinary and plasma osmolalities and the levels of sodium, potas-sium, chloride, paraaminohippuric acid (PAH) and creatinine were measured. Heart rate (HR) and mean arterial pressure (MAP) were also measured. The results were as follows. 1) There were no statistically significants in HR and MAP in all groups. 2) The levels of UV, UNaV, UKV, VCIV, RPF and CH₂O decreased significantly by low dose of fentanyl, not changed by moderate dose of fentanyl and the levels of UV and CH₂O increased signifi-cantly by large dose of fentany. 3) Naloxone pretreatment blocked all of the observed renal responses of low dose of fentanyl. 4) There was no statistically signficant change in renin secretion rate in all groups. From the above results, it is suggested that the fentanyl has dual effects on the renal function and may have systemic effects through opioid receptors.
Halothane, Enflurane, Thalamonal 마취 및 수술이 신기능에 미치는 영향
김미희,송희선,조경우,이상귀,김경숙,설경환,김선희 대한마취과학회 1989 Korean Journal of Anesthesiology Vol.22 No.1
To investigate the changes in renal function during the halothane, enflurane and thalamonal anesthesia and elective surgery, the authors measured urine flow rate, creatinine clearance(Ccr, GFR), excreted amounts of sodium, potassium and chloride ions, fractional excretion of sodium (FeNa), free water clearnace (C H2O) at preanesthesia (control), 20 minute after the induction of anesthesia, during operation (3 times), 1 hour after surgery, respectively, and obtained the results as follows: 1) Changes in renal function was not significant after the induction of anesthesia compared to preanesthesia in halothane, enflurane and thalamonal anesthesia. 2) Renal function decreased signifi-cantly during the operation under anesthesia with halothane or enfiurane. 3) There was a tendency of renal function to be decreased compared to preanesthesia in the 1st postoperative day in patients anesthetized with halothane or enflurane, but tendency of it to be increased in thalamonal anesthesia. Therefore, it is suggested that thalamonal anesthesia is a good choice in patients with renal dysfunc-tion.
Hung Thuan Tran,Young-Joo Park,Mi-Kyeoung Cho,김동진,안대희 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.3
The purpose of this work was to evaluate the development of the anammox process by the use of granular sludge selected from a digestion reactor as a potential seed source in a lab-scale UASB (upflow anaerobic sludge blanket) reactor system. The reactor was operated for approximately 11 months and was fed by synthetic wastewater. After 200 days of feeding with NH4+ and NO2- as the main substrates, the biomass showed steady signs of ammonium consumption, resulting in over 60% of ammonium nitrogen removal. This report aims to present the results and to more closely examine what occurs after the onset of anammox activity, while the previous work described the start-up experiment and the presence of anammox bacteria in the enriched community using the fluorescence in situ hybridization (FISH) technique. By the last month of operation, the consumed NO2--N/NH4+-N ratio in the UASB reactor was close to 1.32, the stoichiometric ratio of the anammox reaction. The obtained results from the influent-shutdown test suggested that nitrite concentration would be one key parameter that promotes the anammox reaction during the start-up enrichment of anammox bacteria from granular sludge. During the study period, the sludge color gradually changed from black to red-brownish.
Differential Distribution of Ganglioside GM3 in Seminiferous Tubule and Epididymis of Adult Rats
Jung, Kyu-Yong,Kim, Bo-Hyun,Cho, Mi-Ran,Kim, Hyoung-Min,Lee, Young-Choon,Kim, Cheorl-Ho,Kim, Jin-Kyeoung,Kim, Byung-Jin,Choo, Young-Kug The Pharmaceutical Society of Korea 2001 Archives of Pharmacal Research Vol.24 No.4
Gangliosides are ubiquitous membrane components in mammalian cells and are suggested to play important roles in various functions such as cell-cell interaction, adhesion, cell differentiation, growth control and signaling. Among all ganglio-series gangliosides, GM3 has the simplest carbohydrate structure, and has been shown as a major gangliosides, in male reproductive system. To study GM3 distribution in the seminiferous tubule and epididymis, frozen sections were stained with specific monoclonal antibody (MAb) against ganglioside GM3. In the seminiferous tubule of testis, pachytene spermatocytes and spermmatids expressed ganglioside GM3, but not in spermatogonia and sertoli cells. Spermatogonia and sertoli cells near the basement membrane were negatively reacted to anti-GM3. In the epididymis, GM3 was expressed only in some interstitial cells. Taken togethers, these results suggest that the expression of ganglioside GM3 in rat seminiferous tubule and epididymis is spatio-temporally regu lated during spermatogenesis.
Tran, Hung-Thuan,Park, Young-Joo,Cho, Mi-Kyeoung,Kim, Dong-Jin,Ahn, Dae-Hee The Korean Society for Biotechnology and Bioengine 2006 Biotechnology and Bioprocess Engineering Vol.11 No.3
The purpose of this work was to evaluate the development of the anammox process by the use of granular sludge selected from a digestion reactor as a potential seed source in a lab-scale UASB (upflow anaerobic sludge blanket) reactor system. The reactor was operated for approximately 11 months and was fed by synthetic wastewater. After 200 days of feeding with $NH_4^+\;and\;NO_2^-$ as the main substrates, the biomass showed steady signs of ammonium consumption, resulting in over 60% of ammonium nitrogen removal. This report aims to present the results and to more closely examine what occurs after the onset of anammox activity, while the previous work described the start-up experiment and the presence of anammox bacteria in the enriched community using the fluorescence in situ hybridization (FISH) technique. By the last month of operation, the consumed $NO_2^--N/NH_4^+-N$ ratio in the UASB reactor was close to 1.32, the stoichiometric ratio of the anammox reaction. The obtained results from the influent-shutdown test suggested that nitrite concentration would be one key parameter that promotes the anammox reaction during the start-up enrichment of anammox bacteria from granular sludge. During the study period, the sludge color gradually changed from black to red-brownish.