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      • Alkylhalide가 Mouse의 LDH isozyme 분포에 미치는 영향 : Cellulose Acetate Electrophoresis Method

        권미경,김영옥,박은주,이동화,이명미,이문희,이양자 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

        The effect of Carbontetrachloride administration on Lactatedehydrogenase isozyme patterns were analyzed by cellulose acetate electrophoresis for the brain, heart, kidney, lung, liver and mouse muscle. Each other tissue was found to have a characteristic distribution of these isozymes. At normal condition, aerobic tissue such as brain, heart, kidney, and lung were found to have all five isozymes from LDH_1 to LDH_5, while anaerobic tissues such as liver and muscle were found to have superiorly LDH_5 The toxicity of Carbontetrachloride administration was most prominence in the liver, and lung toxicity was occured also.

      • 원료콩의 증자조건을 달리한 검정콩 간장의 유리당, 유기산 및 지방산의 변화

        권선화,최재훈,고영란,손미예,박석규 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3

        콩의 증자조건에 따른 검정콩 전통간장의 숙성 후 유리당, 유기산, 지방산조성을 조사하였다. pH 값은 5.04~5.32로 시험구간의 큰 차이를 나타내지 않았고, 원료 콩을 상압증자한 검정콩 간장이 가압이나 스팀보다는 약간 높은 수치를 나타내었다. 적정산도는 4.26~7.73mL로 나타났으며, 가압증자한 검정콩 간장(4.26~5.61mL)이 가장 높았으며, 다음으로 상압(7.32mL), 스팀(5.99~6.96mL)의 순으로 나타났다. 환원당은 25.18~194.81㎎%로 나타났고, 상압이 25.18㎎%로 가장 낮았으며, 가압이나 스팀증자한 검정콩 간장의 경우 상호간에는 큰 차이가 없지만, 상압한 것보다는 7~8배 높은 함량을 나타내었다. 주요 유리당은 glucose, fructose, xylose였으며, 스팀증자한 검정콩 간장이 상압이나 가압보다 각각의 유리당 모두가 많았고, 상압증자는 유리당이 극히 미량으로 검출되었다. 주요 유기산은 succinic acid, oxalicaicd, tartaric aic, malic acid, citric acid였고, 총 유기산은 스팀증자 (40.38~76.96 ㎎%)한 검정콩 간장이 상압(55.85㎎%)이나 가압(27.78~34.21)한 것보다 많았다. 증자 처리시간이 길수로 가압과 스팀으로 처리하여 제도된 검정콩 간장은 약간씩 감소되는 경향을 나타내었다. 주요 지방산은 palmitic acid(33.28~43.51%), stearic a창(14.15~19.16%), myristic acid(5.03~19.14%)였으며, 전체 지방산 중에 55.97~72.85% 비율을 차지하였고, 원료 콩에 존재하지 myristic acid가 상당히 많이 존재하였다. pH values in all samples were in the range of 5.04 to 5.32. pH of kanjang prepared with normal pressure(HPK) was higher than that of kanjang prepared with high pressure(HPK) and kanjang prepared with steam(SPK). Titratable acidity(TA) in all samples were found to be 4.26∼7.72 mL. TA in HPK(5.99∼6.96 mL) was lower than that in SPK(4.26∼5.61 mL) and NPK(7.32 mL). Reducing sugar(RS) contents in all samples were in the range of 25.48 to 194.81 ㎎%. Rs contents of HPK and SPK were a similar pattern and were higher than that of NPK. Major free sugars(FS) were glucose, fructose and xylose. In total FS, SPK was higher than that of NPK and HPK. Major organic acids(OA) were succinic acid, oxalic acid, tartaric acid, malic acid citric acid. OA contents in HPK and SPK were decreased slightly according to the heating time of whole black bean. The highest percentage of fatty acid(FA) in all samples was palmitic acid(33.28∼43.51% as area % of GC chromatogram), followed in a decreasing order by stearic acid(14.15∼19.16%) and myristic acid(5.93∼19.14%). The composition ratios of three FA to total FA ranged from 55.97 to 72.85%. In all samples, there was myristic acid that was not contained in raw soybean.

      • Characteristics of benthic macroinvertebrate communities at reference streams

        Mi-Jung Bae,Hyunduk Cho,Tae-Sung Kwon,Jung Hwa Chun,Young-Seuk Park 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.04

        Benthic macroinvertebrates are sedentary and have an intermediate life span ranging from months to a few years. They play a key role as consumers in the food trophic structure by linking producers, top carnivores, and decomposers in aquatic communities. Therefore, they have been widely used for ecological assessment of aquatic ecosystem health in an integrative and continuous manner. In this study, we characterized benthic macroinvertebrate communities at reference sites, which are not disturbed. Benthic macroinvertebrates were collected seasonally with a Surber net at three different streams (Pocheon, Hongcheon, and Namhae). In addition, hydrological and physicochemical environmental factors including temperature, discharge, width, conductivity, dissolved oxygen and pH were also measured at each sampling site. Spatial and temporal differences of benthic macrointertebrate communities were analyzed based on community indices, functional guilds, etc. relating to their environmental factors. Multivariate analysis were used to characterize the relationships between communities and environmental factors. Benthic macroinvertebrates were significantly different among sampling sites reflecting differences of their environmental condition such as hydromorphological factors, meteorological factors, etc.

      • Variation of benthic macroinvertebrate communities at four different headwater streams

        Mi-Jung Bae,Jung Hwa Chun,Tae-Sung Kwon,Young-Seuk Park 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10

        Headwater streams are the main sources of water sediment and organic material for downstream reaches and their small catchments coupled terrestrial-aquatic linkages. Because headwater streams provide valuable habitats for unique and diverse assemblages of aquatic fauna, they are also essential for sustaining the structure and function of the watersheds. Therefore, it has been recently supported that headwaters are important areas for maintaining biodiversity. In this study, we surveyed four different headwater streams in different watersheds to characterize differences of benthic macroinvertebrate assemblages among study streams. Benthic macroinvertebrates were collected seasonally with a Surber net at four different streams. In addition, hydrological and physicochemical environmental factors including temperature, discharge, width, conductivity, dissolved oxygen and pH were also measured at each sampling site. Community indices were used to differentiate spatiotemporal changes of macrointertebrate communities. Multivariate analysis were used to characterize the relationships between communities and environmental factors. Differences of their environmental condition such as meteorological factors and stream hydromorphological factors were reflected in the ordination with macroinvertebrate communities.

      • Larvicidal activity of medicinal plant extracts and lignan identified in Phryma leptostachya var. asiatica roots against house fly (Musca domestica L.)

        Il-Kwon Park,Seon-Mi Seo,Yoon-Mi Jeon,Sung-Woong Kim,Hwa-Jeong Yeom,Hyo-Rim Lee 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

        Medicinal plant extracts from 27 plant species in 20 families were tested for their larvicidal activity against house fly, Musca domestica (L.). Responses varied with plant material and concentration. Among plant species tested, Phryma leptostachya var. asiatica showed 100% larvicidal activity against M. domestica at 10 mg/g concentration. Larvicidal activities of Atractylodes japonica, Saussurea lappa, Asiasarum sieboldi, and Gleditsia japonica var. koraiensis were 89.3, 85.3, 93.3 and 96.6% at 10 mg/g concentration, respectively. Extracts of Prunus persica, Curcuma longa and Paeonia moutan produced moderate activity. Larvicidal activity of other plant extracts was less than 50%. Among test plant species, Phryma leptostachya var. asiatica showed the most potent larvicidal activity. The active constituent of P. leptostachya var. asiatica roots was identified as the leptostachyol acetate by spectroscopic analysis. The LC50valuesofleptostachyolacetateagainstM. domestica larvae was 0.039 mg/g. Naturally occurring medicinal plant extracts and Phryma leptostachya var. asiatica root–derived compounds merit further study as potential house fly larval control agents or lead compounds.

      • Growth of the Box Tree Pyralid, Glyphodes perspectalis Reared on an Artificial Diet Through Four Generations

        Il-Kwon Park,Sung-Woong Kim,Hwa-Jung Yeom,Yoon-Mi Jeon,Seon-Mi Seo,Sang-Chul Shin 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10

        The box tree pyralid, Glyphodes perspectalis, was reared on an artificial diet based on dried powder of box tree leaves, Buxus microphylla var. koreana, and INSECTA F-II (Nihon Nosan Co., Ltd) at a ratio of 3:7 (w:w) and analyzed in their developmental characteristics from 1st to 4th generation. The developmental period of larva reared on artificial diet was longer than that of larva reared on host plant. Significant difference in larval period was also observed between 1st and other generation groups. Pupation rate was the highest in 4th generation followed by 3rd, 2nd, 1st and host plant group. Significant difference in pupal period was noted between 1st and 3rd generation group. Emergence rate was the highest in 4th generation group followed by 3rd, host plant, 1st and 2nd generation group. There is a significant difference in male and female size between 1st and 4th generation group. Our result indicated that stable rearing of box tree pyralid through the year is possible by artificial diet, and the best adaptation to artificial diet was achieved at 4th generation.

      • KCI등재
      • 백작약 열수추출물의 피부노화 억제 효과(Ⅰ)

        권미화(Mi-Hwa Kwon),민경진(Kyung-Jin Min),김영철(Young-Chul Kim) 환경독성보건학회 2009 환경독성보건학회지 Vol.24 No.2

        To investigate the alleviative effects of Peonia japonica water extract (PJWE) on inflammation and skin barrier damage, both the irradiation of UVB and the application of squalene monohydroperoxide (Sq-OOH) to the backs of hairless mice were performed for 4 weeks. And at the same time experimental materials were applied topically. The skin erythema indices for the positive control (PC, 0.01% retinoic acid) and experimental (E, PJWE) groups were lower than that of the control (C) group. Whereas both the lipid and water capacities for the PC and E groups were higher than those of the C group. Epidermis and dermis of the C group were remarkably thickened in comparison with the PC and E groups. Relatively much less number of inflammatory cells, including lymphocytes, neutrophils and macrophages were found in dermis of the PC and E groups compared with the C group. Lipid lamellae of the C group were broken severely showing an irregular arrangement and lipid content was much reduced. Whereas those of the PC and E groups were almost intact with a regular arrangement, which were similar to that of the N group. Taken the results all together, it was confirmed that PJWE could be effective natural herbal material for the alleviation of inflammation and skin barrier damage in hairless mice skin which were induced by UVB and Sq-OOH.

      • KCI등재

        발달장애학생 학부모의 장애인활동보조서비스의 이용 현황과 이용 후 인식

        권미영 ( Mi Young Kwon ),황미화 ( Mi Hwa Hwang ) 한국특수교육문제연구소 2016 특수교육저널 : 이론과 실천 Vol.17 No.2

        본 연구는 장애인의 일상생활 지원과 자립을 목적으로 실시하고 있는 장애인활동보조서비스에 대해 이용자인 발달장애학생 학부모의 인식을 조사하고 분석하여, 장애학생과 가족의 필요욕구를 만족시킬 수 있는 실질적이고 효과적인 정책수립을 위한 기초자료를 제시하는 것을 목적으로 한다. 이를 위해 D 광역시와 U 광역시 등 6개 도시에서 재학 중인 발달장애학생의 학부모 305명을 대상으로 설문조사를 실시하였다. 이러한 설문조사를 SPSS 21.0 통계프로그램을 사용하여 빈도분석, T-검정, 일원배치분산분석(oneway Anova), 요인분석을 실시하였고, 발달장애학생 학부모의 이용 현황과 이용 후 인식에 대해 분석하였다. 연구 결과를 요약하면 첫째, 발달장애학생의 학부모는 장애인활동보조서비스를 5년 이상 이용하였고, 일주일에 4회 이상, 가족들의 부담을 줄여주기 위해 학부모 스스로가 신청하여 이용하였다가 많았다. 둘째, 발달장애학생의 학부모는 변화와 도움에 대해 장애인활동보조서비스를 이용 후 집안일의 부담감소와 타인과의 의사소통이 증가하였고, 학생의 청결상태와 식사하기 및 외출이 증가하였다고 긍정적으로 인식하고 있었다. 또한 갈등에 대해 활동보조인의 간섭과 힘든 일을 꺼려하는 것을 부정적으로 인식하고 있었으며, 이용영역에 대해 사회활동서비스와 교육과 훈련서비스의 이용이 많아서 이에 대해 필요를 더 많이 인식하고 있었다. 그러므로 활동보조서비스를 이용하는 학부모들의 요구를 반영한 지원을 위해 만족도를 분석하고 수요자의 필요와 특성에 맞는 지원방안을 제시할 수 있는 연구와 정책이 요구된다. The purpose of personal assistance service for the people with disabilities is the support of their basic daily life and independence. This study was performed to present the practical data which the support for the people with disabilities can be carried out effectively to satisfy the needs of the consumer, through the statistical analysis of the satisfaction and the needs of parents of students with developmental disabilities who use this service. For this purpose 305 questionnaires was performed for parents of students with developmental disabilities in the six metropolitan cities including D and U metropolitan city. Using the SPSS 21.0 statistical program, the recognitions about personal assistance service of parents of students with the developmental disabilities were analyzed through the frequency analysis T- test, one-way Anova, and the factor analysis. Major findings of this study were as follows. First, most of parents of students with developmental disabilities had been used personal assistance service over four times a week and over five years. Second, parents of students with developmental disabilities referred increasing of the communication with others and reducing the burden of housework, which were changed after using personal assistance service. Third, cleanliness, eating, and going out of students with developmental disabilities seemed to have been helpful. Fourth, parents were thinking interference and reluctance to hard work as a conflict factor of this service. Finally, social activity services and education and training services were referred to as necessary use area. Therefore, through the analysis of satisfaction for the support that reflects the needs of parents using the PAS, the research and the policy are required for the support measures that may be presented to meet the needs and characteristics of each individual consumer.

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