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Md. Shihabul Islam,Chowdhury Arif Jahangir,Md. Sifat Rahi,Md. Mahmudul Hasan,Salek Ahmed Sajib,Kazi Md. Faisal Hoque,Md Abu Reza 한국독성학회 2020 Toxicological Research Vol.36 No.1
Cancer is the second death causing disease all over the world and until today 100 different types of cancer have been identified whose treatment methods consist of serious side effects on human body. To reduce the frequency of adverse effects of cancer treatment, nowadays plant derived natural components are getting priority. The plant Morus latifolia is widely available in northern part of Bangladesh. The earlier researches suggested that popular varieties of some Morus sp. like Morus alba, Morus indica etc. have good anti-proliferative activity. Hence, this study was designed to evaluate the anti-proliferative activity of leaf and bark extracts of M. latifolia against Ehrlich’s ascites carcinoma (EAC) in vivo. The leaf and bark extracts of M. latifolia were used in several bioassays including Brine shrimp lethality test, hemagglutination activity test, antioxidant activity test, and cell growth inhibition test. Besides, fluorescence microscopy was performed to study apoptotic features in EAC cells, and molecular analysis like real-time PCR were also conducted. The results of Brine shrimp lethality test, hemagglutination activity test, and antioxidant activity assay supported the cell growth inhibition capability of leaf and bark extracts which was confirmed by in vivo cell growth inhibition bioassay. Moreover, the experimental extracts were able to induce cell apoptotis through altering the expression pattern of Bcl-2 and Bax genes. All of the results of this study suggest that several noble compounds are present in M. latifolia plant extracts which are capable for healing cancer cells.
Anther Culture in Crop Plants: Progress and Perspectives
M. Thoihidul Islam(M. Thoihidul Islam ),Mohammad Rashid Arif(Mohammad Rashid Arif ),Md. Toufiq Hasan(Md. Toufiq Hasan ),Arif Hasan Khan Robin(Arif Hasan Khan Robin ) 한국육종학회 2023 Plant Breeding and Biotechnology Vol.11 No.2
A resurrection has started in haploid and double haploid research in the twenty-first century. The haploid and double haploid could be achieved through in vivo and in vitro anther and microspore culture techniques. Fixing the homozygosity is the most striking benefit of androgenesis. Various factors like genotypic dependency, growth condition, developmental stage of the microspore, pre-treatment, culture media, regeneration media, growth hormones, and various chemicals have a direct effect. Wheat, rice, Brassica, and tobacco are the notable crops where anther and microspore culture has been utilized. These haploidy and double haploidy through anther culture served many purposes of basic and applied research. Especially, double haploid cultivars have been cultivating around the globe. In addition, for chromosome mapping, QTL mapping, marker-assisted selection, marker-assisted backcrossing, mutation breeding, genome-wide association study, genomic engineering, and genome editing, androgenesis based haploid and double haploid plants have been exploited due to the effectiveness. Recently, researchers are trying to explain albinism that happens during anther culture from an epigenetic perspective. Further prospects of haploid and doubled haploid research through anther culture have been described in this review.
( Md. Selim Arif Sher Shah ),유필진 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0
Ternary composites of reduced graphene oxide (RGO), SnO2 and carbon were synthesized at low temperature without heat treatment at high temperature. In the composites size of SnO2 nanoparticles are 2.5 nm. Graphene oxide was reduced to appreciable extent at this temperature by ascorbic acid which also serves as carbon source. The composites and the presence of carbon therein were proved through several techniques like, Raman spectroscopy, thermogravimetric analysis, transmission electron microscopy and energy dispersive X-ray analysis. The ternary composite shows specific capacity slight excess over the theoretical capacity (1493 mAh/g) of SnO2 at 100 mA/g after 100 cycles and a Coulombic efficiency >99%. Moreover, it displays good rate performance. Cyclic voltammetry proves that the conversion reaction (reduction of SnO2 with Li+ to produce Sn and the synchronous formation of Li2O) is reversible in the ternary composite whereas it is irreversible in SnO2 and binary composites. High specific capacity and good rate performance were attributed to the quantum dot sized SnO2 nanoparticles which shortens Li ion diffusion length, effective accommodation of volume change offered by RGO and carbon and reversibility of the conversion reaction.
Arif Sher Shah, Md. Selim,Zhang, Kan,Park, A. Reum,Kim, Kwang Su,Park, Nam-Gyu,Park, Jong Hyeok,Yoo, Pil J. The Royal Society of Chemistry 2013 Nanoscale Vol.5 No.11
<P>With growing interest in the photocatalytic performance of TiO2-graphene composite systems, the ternary phase of TiO2, graphene, and Ag is expected to exhibit improved photocatalytic characteristics because of the improved recombination rate of photogenerated charge carriers and potential contribution of the generation of localized surface plasmon resonance at Ag sites on a surface of the TiO2-graphene binary matrix. In this work, Ag-TiO2-reduced graphene oxide ternary nanocomposites were successfully synthesized by a simple solvothermal process. In a single-step synthetic procedure, the reduction of AgNO3 and graphene oxide and the hydrolysis of titanium tetraisopropoxide were spontaneously performed in a mixed solvent system of ethylene glycol, N,N-dimethylformamide and a stoichiometric amount of water without resorting to the use of typical reducing agents. The nanocomposites were characterized by X-ray diffraction, X-ray photoelectron spectroscopy, along with different microscopic and spectroscopic techniques, enabling us to confirm the successful reduction of AgNO3 and graphite oxide to metallic Ag and reduced graphene oxide, respectively. Due to the highly facilitated electron transport of well distributed Ag nanoparticles, the synthesized ternary nanocomposite showed enhanced photocatalytic activity for degradation of rhodamine B dye under visible light irradiation.</P>
Md Zahid Hassan,Arif Hasan Khan Robin,Md Abdur Rahim,Sathishkumar Natarajan,김효택,박종인,노일섭 한국식물생명공학회 2018 JOURNAL OF PLANT BIOTECHNOLOGY Vol.45 No.3
Gummy stem blight (GSB) is one of the most destructive and economically important, soil borne diseases of melon caused by the ascomycete fungus, Didymella bryoniae throughout the world. In Korea, however, no GSB resistant genotype has been reported yet. The study aimed to identify GSB resistant melon germplasm. We screened a total of 60 genotypes including 16 lines and 44 melon cultivars collected from USA and Korea. Among the 16 melon lines, four lines including ‘PI482399’, ‘PI140471’, ‘PI136170’ and ‘PI420145’, and two Korean cultivars viz. ‘Asia Papaya’ and ‘Supra’ showed complete resistance. We were aware that both genotypic and environmental variations could influence the phenotypic screening of resistance and susceptibility. We therefore, further assessed all genotypes using 20 SSR markers. The SSR marker ‘CMCT505’ linked to Gsb1 in chromosome 1 perfectly grouped resistant and susceptible lines indicating that resistance is probably due to the presence of Gsb1 gene. Cloning and sequencing of resistant and susceptible Gsb1 amplicons showed that there were 32-bp deletions in resistant line and 39-bp deletions in resistant cultivar compared to susceptible one. Thus, the resistant melon lines and cultivars identified in this study could be recommended for the melon breeding program. Furthermore, the SSR marker ‘CMCT505’ which is tightly linked with Gsb1 could be used for molecular screening of melon germplasm.