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MicroRNA-7641 as a Promising Targeting Factor to Improve the Efficacy of Cancer Therapy
Abu Musa Md Talimur Reza,Jae woong Han,Yun-jung Choi 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2
Many diseases, including myocardial infarction, autoimmune disease, viral diseases, neurodegenerative diseases, and cancers, are frequently diagnosed with the aberrant expression of microRNAs (miRNAs) and their allied pathways. This indicates the crucial role of miRNAs in maintaining biological and physiological processes. miR-7641 is a miRNA whose role in disease has not been fully investigated. In the present study, we investigated the expression pattern of miR-7641 and its target genes in different cancer cells, as well as in patients with breast cancer and colorectal cancer. Direct inhibition of miR-7641 using a locked nucleic acid upregulated the expression of its target genes, sensitized cancer cells, and enhanced the efficiency of therapeutic agents such as doxorubicin. In addition, inhibition of miR-7641 boosted doxorubicin-mediated apoptosis of cancer cells via upregulation of apoptotic molecules Caspase 9 (CAS9) and poly ADP ribose polymerase (PARP) and downregulation of anti-apoptotic molecule BCL2. Thus, these findings indicated that miR-7641 is a clinically important therapeutic target for cancers. Inhibition of miR-7641 expression could be an efficient treatment strategy for patients with breast and colorectal cancer. In addition, inhibitors of miR-7641 could be used in combination with other therapeutic agents, such as doxorubicin, to enhance the efficiency of cancer therapy.
Md. Mahmudul Hasan,Md. Shihabul Islam,Kazi Md. Faisal Hoque,Ariful Haque,Md Abu Reza 한국독성학회 2019 Toxicological Research Vol.35 No.3
Citrus macroptera (Rutaceae) has long been used in folk medicine in Bangladesh. Considering the folkloric context, this study was aimed to scrutinize anti-proliferative activity of C. macroptera fruit pulp juice (CMFPJ) against Ehrlich’s ascites carcinoma (EAC). The anti-proliferative capacity of CMFPJ was investigated and confirmed primarily using MTT assay. In vivo anti-proliferative aptitude of CMFPJ was investigated with 25, 50, and 100 mg/kg/day intraperitoneal (i.p.) treatment. Anti-proliferative efficacy of CMFPJ was assessed based on EAC growth inhibition. CMFPJ inhibited EAC growth in vitro in a dose-dependent manner. And the percentages of in vivo EAC growth inhibition were 19.53, 49.2, and 68.9% at 25, 50, and 100 mg/kg CMFPJ respectively. CMFPJ significantly induced expression of apoptosis regulatory genes caspase-8, caspase-9, cytochrome-c, and caspase-3. This considerable anti-cancer activity was perhaps due to combinatorial effect of lectin, polyphenols, and flavonoids present in CMFPJ.
Md. Shihabul Islam,Chowdhury Arif Jahangir,Md. Sifat Rahi,Md. Mahmudul Hasan,Salek Ahmed Sajib,Kazi Md. Faisal Hoque,Md Abu Reza 한국독성학회 2020 Toxicological Research Vol.36 No.1
Cancer is the second death causing disease all over the world and until today 100 different types of cancer have been identified whose treatment methods consist of serious side effects on human body. To reduce the frequency of adverse effects of cancer treatment, nowadays plant derived natural components are getting priority. The plant Morus latifolia is widely available in northern part of Bangladesh. The earlier researches suggested that popular varieties of some Morus sp. like Morus alba, Morus indica etc. have good anti-proliferative activity. Hence, this study was designed to evaluate the anti-proliferative activity of leaf and bark extracts of M. latifolia against Ehrlich’s ascites carcinoma (EAC) in vivo. The leaf and bark extracts of M. latifolia were used in several bioassays including Brine shrimp lethality test, hemagglutination activity test, antioxidant activity test, and cell growth inhibition test. Besides, fluorescence microscopy was performed to study apoptotic features in EAC cells, and molecular analysis like real-time PCR were also conducted. The results of Brine shrimp lethality test, hemagglutination activity test, and antioxidant activity assay supported the cell growth inhibition capability of leaf and bark extracts which was confirmed by in vivo cell growth inhibition bioassay. Moreover, the experimental extracts were able to induce cell apoptotis through altering the expression pattern of Bcl-2 and Bax genes. All of the results of this study suggest that several noble compounds are present in M. latifolia plant extracts which are capable for healing cancer cells.
Abu Musa Md Talimur Reza,Jae woong Han,Yun-Jung Choi 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2
Recombination activating gene-2 (Rag2) participate in the immune system process through V(D)J recombination of B-cells and T-cells activation process. The deletion of Rag2 gene impaired the immune regulation through complete loss of matured B-cells and T-cells. However, in this research we found that Rag2-ablated mice showed very marginal expression for both B-cells (CD20 and CD79a) and T-cells (CD4 and CD8) markers. These data indicated the possibility of leakiness of B-cells and T-cells at least in a marginal level in the spleen and thymus of Rag2-deficient mice. It also suggested that effectiveness of Rag2 deletion could be regulated based on the strain and age of the concerned mice. Thereafter, we investigated the miRNAs and genes expression profile of Rag2 knockout mice spleen and found that some of the deregulated genes are putative targets of the differentially expressed miRNAs and might be transcriptionally connected. Moreover, the deregulated miRNAs and genes are also actively involved in both B-cells and T-cells activation signaling network. Finally, we concluded that miRNAs along with the related genes are important regulator of B-cells and T-cells signaling cascade, and the Rag2 deletion might impaired B-cells and T-cells development in a miRNAs-dependent manner.
Hasan, Md. Mahmudul,Islam, Md. Shihabul,Hoque, Kazi Md. Faisal,Haque, Ariful,Reza, Md Abu Korean Society of ToxicologyKorea Environmental Mu 2019 Toxicological Research Vol.35 No.3
Citrus macroptera (Rutaceae) has long been used in folk medicine in Bangladesh. Considering the folkloric context, this study was aimed to scrutinize anti-proliferative activity of C. macroptera fruit pulp juice (CMFPJ) against Ehrlich's ascites carcinoma (EAC). The anti-proliferative capacity of CMFPJ was investigated and confirmed primarily using MTT assay. In vivo anti-proliferative aptitude of CMFPJ was investigated with 25, 50, and 100 mg/kg/day intraperitoneal (i.p.) treatment. Anti-proliferative efficacy of CMFPJ was assessed based on EAC growth inhibition. CMFPJ inhibited EAC growth in vitro in a dose-dependent manner. And the percentages of in vivo EAC growth inhibition were 19.53, 49.2, and 68.9% at 25, 50, and 100 mg/kg CMFPJ respectively. CMFPJ significantly induced expression of apoptosis regulatory genes caspase-8, caspase-9, cytochrome-c, and caspase-3. This considerable anti-cancer activity was perhaps due to combinatorial effect of lectin, polyphenols, and flavonoids present in CMFPJ.
Graphene Oxide Size Dependent Toxicity to TM3, TM4 and Spermatogonial Stem Cell
Jae woong Han,Abu Musa Md Talimur Reza,Yun-Jung Choi 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2
Graphene oxide (GO) is a novel material derived from two-dimentional carbon lattice of graphene and has attracted increasing interest in the field of biomedicine as grug delivery, gene delivery, biosensers. Also G.O is well known that low toxicity in cells but effect in germ cell development are poorly understood. Leydig cells and Sertoli cells are somatic cells that surpport germ cells during spermatogenesis. In this study, we found that toxicity to leydig and sertoli cell line induced by G.O following variants size. The small size (about 27 nm) showing more high toxicity and cell damage, apoptosis in those somatic cells compare with large size (about 80 nm). Small size G.O is more easy to upkate to cells and effect to cells. Taken together, our study reveals that G.O showing cell toxicity, even if low, and possibility that interrupt spermatogenesis by damage of leydig and sertoli cells. It is important information that apply small size of G.O as biomeical drug.
Low frequency stability study of a three-phase induction motor
M. Bashir Uddin,Md. Nuruzzaman Pramanik,Sheikh Abu Reza 전력전자학회 2007 ICPE(ISPE)논문집 Vol.- No.-
A suitable volt/㎐ control strategy is proposed for stability improvement in low frequency operation of induction motors fed from variable voltage and variable frequency supply. The analysis is based on small scale perturbation method applied to the linearized model of state-space equations. Stability analyses are carried out through evaluation of eigen values of the linear model characteristic equation. Stability investigation shows that the simultaneous adjustment of stator voltage and frequency provides a convenient means for maintaining stability of induction motor. The results are verified by standard transient analysis method and demonstrate a very close agreement.
Jae woong Han,Abu Musa Md Talimur Reza,Yun-Jung Choi 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2
Silver nanoparticles (AgNPs) is one of the nanomaterials as most popular and commonly used in consumer products in biomedical devices, antibiotic agent, wound dressings, text tile and also candidate of anti-cancer material. F9 cell, the teratocarcinoma stem cell line has been widely used as a model for the analysis of molecular mechanisms associated with differentiation and retinoic acid (R.A) is well known molecule that induce F9 cell differentiation. There are many study about induce differentiation in stem cells by AgNPs and in anti-cancer therapy, induce cancer stem cell differentiation is very important to killing cancer. In this study, we demonstrated that AgNPs showing toxicity in F9 cells as anti-cancer cell effect and also induce F9 cell differentiation. F9 cells are induce cell toxicity and apoptosis after treated AgNPs and also make F9 cell differentiation like induced by R.A. Signaling pathway analysis showing that gene and protein expression going different and more sensetive to anti cancer drug, cisplatin, after induced differentiation by AgNPs. We found that potential of AgNPs as not only killing cancer but also make cancer stem cell going to differentiation.