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Maria John Muthaiya,PraveenNagella,MuthuThiruvengadam,AbulKalam Azad Mandal 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.2
Tea cell suspension culture is an alternative method for the synthesis of secondary metabolites. For the separation of cells, different concentrations of pectinase were used and a concentration of 0.5% was found to be the optimum concentration for the separation of cells (41.7%) in the culture medium than the other two concentrations (33.3 and 25.0%). The separated cells were cultured in liquid MS medium using different PGR combinations. The time taken for the cells to reach stationary phase, under different PGRs,ranged from 17 to 21 d. The maximum cell density was found in IAA and 2, 4-D medium at 21 d followed by 2, 4-D. Results revealed that the amount of secondary metabolites such as catechins were high with stationary phase when compared to other growth phases (lag and log phases). Different concentrations of shikimic acid (10, 20, and 30 mM) were added to the stationary phase of cell culture in the bioreactor and the secondary metabolite content was analyzed. Synthesis of polyphenols, catechins, caffeine, and other secondary components were high (33.87, 22.85, and 4.66%) with 20 mM shikimic acid treatment than the other two concentrations
Maria Sfakianaki,Chara Papadaki,Maria Tzardi,Maria Trypaki,Sardar Alam,Eleni D. Lagoudaki,Ippokratis Messaritakis,Odysseas Zoras,Dimitris Mavroudis,Vassilis Georgoulias,John Souglakos 대한암학회 2019 Cancer Research and Treatment Vol.51 No.4
Purpose The purpose of this study was to investigate the prognostic significance of liver kinase b1 (LKB1) loss in patients with operable colon cancer (CC). Materials and Methods Two hundred sixty-two specimens from consecutive patients with stage III or high-risk stage II CC, who underwent surgical resection with curative intent and received adjuvant chemotherapy with fluoropyrimidine and oxaliplatin, were analyzed for LKB1 protein expression loss, by immunohistochemistry as well as for KRAS exon 2 and BRAFV600E mutations by Sanger sequencing and TS, ERCC1, MYC, and NEDD9 mRNA expression by real-time quantitative reverse transcription polymerase chain reaction. Results LKB1 expression loss was observed in 117 patients (44.7%) and correlated with right-sided located primaries (p=0.032), and pericolic lymph nodes involvement (p=0.003), BRAFV600E mutations (p=0.024), and TS mRNA expression (p=0.041). Patients with LKB1 expression loss experienced significantly lower disease-free survival (DFS) (hazard ratio [HR], 1.287; 95% confidence interval [CI], 1.093 to 1.654; p=0.021) and overall survival (OS) (HR, 1.541; 95% CI, 1.197 to 1.932; p=0.002), compared to patients with LKB1 expressing tumors. Multivariate analysis revealed LKB1 expression loss as independent prognostic factor for both decreased DFS (HR, 1.217; 95% CI, 1.074 to 1.812; p=0.034) and decreased OS (HR, 1.467; 95% CI, 1.226 to 2.122; p=0.019). Conclusion Loss of tumoral LKB1 protein expression, constitutes an adverse prognostic factor in patients with operable CC.
Muthaiya, Maria John,Nagella, Praveen,Thiruvengadam, Muthu,Mandal, AbulKalam Azad 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.2
Tea cell suspension culture is an alternative method for the synthesis of secondary metabolites. For the separation of cells, different concentrations of pectinase were used and a concentration of 0.5% was found to be the optimum concentration for the separation of cells (41.7%) in the culture medium than the other two concentrations (33.3 and 25.0%). The separated cells were cultured in liquid MS medium using different PGR combinations. The time taken for the cells to reach stationary phase, under different PGRs, ranged from 17 to 21 d. The maximum cell density was found in IAA and 2, 4-D medium at 21 d followed by 2, 4-D. Results revealed that the amount of secondary metabolites such as catechins were high with stationary phase when compared to other growth phases (lag and log phases). Different concentrations of shikimic acid (10, 20, and 30 mM) were added to the stationary phase of cell culture in the bioreactor and the secondary metabolite content was analyzed. Synthesis of polyphenols, catechins, caffeine, and other secondary components were high (33.87, 22.85, and 4.66%) with 20 mM shikimic acid treatment than the other two concentrations.
Maria Azrad,Robin T. Vollmer,John Madden,Mark Dewhirst,Thomas J. Polascik,Denise C. Snyder,Mack T. Ruffin,Judd W. Moul,Dean E. Brenner,Wendy Demark-Wahnefried 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.4
Enterolactone and enterodiol, mammalian lignans derived from dietary sources such as flaxseed, sesame seeds, kale, broccoli, and apricots, may impede tumor proliferation by inhibiting activation of nuclear factor kappa B (NFκB) and vascular endothelial growth factor (VEGF). We examined the associations between urinary enterolactone and enterodiol with prostatic tumor expression of NFκB, VEGF, and Ki67 among 147 patients with prostate cancer who participated in a presurgical trial of flaxseed supplementation (30 g/day) for 30 days. Urinary enterolignans and tissue biomarkers were determined by high-performance liquid chromatography and immunohistochemistry, respectively. After supplementation, we observed significant correlations between intakes of plant lignan and urinary concentrations of total enterolignans (ρ=0.677, P<.0001), enterolactone (ρ=0.676, P<.0001), and enterodiol (ρ=0.628, P<.0001). Importantly, we observed that total urinary enterolignans and enterolactone were significantly and inversely correlated with Ki67 in the tumor tissue (ρ=−0.217, P=.011, and ρ=−0.230, P=.007, respectively), and a near-significant inverse association was observed for enterodiol (ρ=−0.159, P=.064). An inverse association was observed between enterolactone and VEGF (ρ=−0.143, P=.141), although this did not reach statistical significance. We did not observe an association between enterolignans and NFκB. In conclusion, flaxseed-derived enterolignans may hinder cancer cell proliferation via VEGF-associated pathways.
Mitral-Aortic Intervalvular Fibrosa Pseudoaneurysm
Maria Bonou,Eva D Papadimitraki,Sophia Vaina,Glafkos Kelepeshis,Kostas Tsakalis,Nikolaos Alexopoulos,John Barbetseas 한국심초음파학회 2015 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.23 No.4
Pseudoaneurysm of the mitral aortic intervalvular fibrosa (MAIVF-P) usually ensues as a complication of endocarditis or aortic valve surgery. When large, symptomatic or related to complications (rupture, compression of adjacent structures, embolic events, mitral regurgitation or heart failure) it warrants surgical excision. The natural course of uncomplicated/asymptomatic MAIVF-Ps is largely unknown since most patients are offered surgery. Increased surgical risk imposed by repeat operations in the majority of these patients is an important consideration and conservative treatment should not be excluded in selected cases. Herein we present two illustrative cases of MAIVF-P manifesting with significant arrhythmogenesis and complex endocarditis respectively. Both patients were managed conservatively. By briefly reviewing the existing literature, we discuss important diagnostic and therapeutic issues for MAIVF-Ps. To our knowledge complex ventricular arrhythmia has not been previously described as a prominent manifestation of MAIVF-P.
Identification and Accurate Quantitation of Biological Oligosaccharide Mixtures
Strum, John S.,Kim, Jaehan,Wu, Shuai,De Leoz, Maria Lorna A.,Peacock, Kyle,Grimm, Rudolf,German, J. Bruce,Mills, David A.,Lebrilla, Carlito B. American Chemical Society 2012 ANALYTICAL CHEMISTRY - Vol.84 No.18
<P>Structure-specific characterization and quantitation is often required for effective functional studies of oligosaccharides. Inside the gut, HMOs are preferentially bound and catabolized by the beneficial bacteria. HMO utility by these bacteria employs structure-specific catabolism based on a number of glycosidases. Determining the activity of these enzymes requires accurate quantitation of a large number of structures. In this study, we describe a method for the quantitation of human milk oligosaccharide (HMO) structures employing LC/MS and isotopically labeled internal standards. Data analysis was accomplished with a newly developed software tool, LC/MS Searcher, that employs a reference structure library to process LC/MS data yielding structural identification with accurate quantitation. The method was used to obtain a meta-enzyme analysis of bacteria, the simultaneous characterization of all glycosidases employed by bacteria for the catabolism of milk oligosaccharides. Analysis of consumed HMO structures confirmed the utility of a β-1,3-galactosidase in <I>Bifidobacterium longum subsp. infantis</I> ATCC 15697 (<I>B. infantis</I>). In comparison, <I>Bifidobacterium breve</I> ATCC 15700 showed significantly less HMO catabolic activity compared to <I>B. infantis</I>.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2012/ancham.2012.84.issue-18/ac301128s/production/images/medium/ac-2012-01128s_0002.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac301128s'>ACS Electronic Supporting Info</A></P>
Mendoza, Maria Krisandra L.,Tongol, Bernard John V.,Shanmugam, Sangaraju,Kim, Hasuck Elsevier 2018 International journal of hydrogen energy Vol.43 No.43
<P><B>Abstract</B></P> <P>Pulse electrodeposition of Pt and Sn using 10 mM H<SUB>2</SUB>PtCl<SUB>6</SUB>⋅6H<SUB>2</SUB>O in 0.10 M H<SUB>2</SUB>SO<SUB>4</SUB> and 10 mM SnCl<SUB>2</SUB>⋅2H<SUB>2</SUB>O in 0.10 M HCl was conducted on a support matrix consisting of electropolymerized poly (3,4-ethylenedioxythiophene) (PEDOT) and electrochemically exfoliated graphene oxide (EGO). The Field Emission – Scanning Electron Microscopy (FE-SEM) studies of PtSn/PEDOT/EGO (i.e., PEDOT on EGO) showed a homogeneous globular composite, while PtSn/EGO/PEDOT (i.e., EGO on PEDOT) revealed a heterogeneous composite with wrinkled and globular surface morphologies. An Energy Dispersive X-ray (EDX) analysis (as a percentage of Pt and Sn) of PtSn/PEDOT/EGO is in agreement with the X-ray Photoelectron Spectroscopy (XPS) analysis, indicative of a homogeneous surface for the dispersion of metallic particles. However, the EDX and XPS analyses of PtSn/EGO/PEDOT showed variations in the amount of Pt and Sn, indicative of possible mixing of the EGO and PEDOT support matrices. Cyclic voltammetry (CV) and chronoamperometry using 1.0 M ethanol in 0.1 M H<SUB>2</SUB>SO<SUB>4</SUB> demonstrated higher electrocatalytic activity (83.7 mA/cm<SUP>2</SUP>) and electrochemical stability (29.0% current retention) in PtSn/PEDOT/EGO than PtSn/EGO/PEDOT.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PtSn was pulse electrodeposited on PEDOT/EGO and EGO/PEDOT support matrices. </LI> <LI> PEDOT/EGO is a better support matrix for the pulse electrodeposited PtSn. </LI> <LI> PtSn/PEDOT/EGO showed a more enhanced electrocatalytic activity and stability. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Device Architectures for Enhanced Photon Recycling in Thin-Film Multijunction Solar Cells
Sheng, Xing,Yun, Myoung Hee,Zhang, Chen,Al-Okaily, Ala'a M.,Masouraki, Maria,Shen, Ling,Wang, Shuodao,Wilson, William L.,Kim, Jin Young,Ferreira, Placid,Li, Xiuling,Yablonovitch, Eli,Rogers, John A. Wiley (John WileySons) 2015 Advanced Energy Materials Vol.5 No.1
Effective Use of Multiple Random Walks in P2P Networks
Zita Maria Almeida do Vale,Carlos Ramos,Rosslin John Robles 사단법인 인문사회과학기술융합학회 2014 예술인문사회융합멀티미디어논문지 Vol.4 No.1
Nowadays, millions of users search and download desired data such as Napster and Gnutella as they are Peer-to-Peer (P2P) files sharing applications. In order to improve performance in unstructured P2Ps replication strategies are used. Efficient and effective full-text retrieval over unstructured p2p networks was developed in order to address the problems of the query popularity independent replication strategies, previously a novel strategy. In order to support random node sampling and network size estimation a lightweight DHT with an unstructured P2P overlay. However these well-organized techniques are executed irrespective of topologies and network size concerns. To overcome this problem, we propose a query algorithm based on multiple random walks that resolve queries almost as quickly as unstructured P2P overlay method while reducing the network traffic by two orders of magnitude in many cases. We also present simulation results on a distributed replication strategy.