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Nagella Siva Gangi Reddy,Kummara Madhusudana Rao,Kummari Subba Venkata Krishna Rao,하창식 한국고분자학회 2016 Macromolecular Research Vol.24 No.6
This paper describes the synthesis of a new chelating poly(acrylamide-co-dimethylaminoethyl methacrylate- co-1-acryloyl-3-phenyl thiourea) (PAPDM) hydrogel. The PAPDM hydrogels were prepared by the simple free radical polymerization of monomers acrylamide, dimethylamino ethyl methacrylate and 1-acryloyl-3-phenyl thiourea. The free radical initiator used for this study was ammonium persulphate and the cross-linker was ethylene glycol di methacrylate. The swelling study of the PAPDM hydrogels were performed in the aqueous environment to calculate the morphological parameters, such as the volume fraction in a swollen gel (v2m), polymer-solvent interaction parameter (χ) and molecular weight between the crosslinks (Mc). These hydrogels were characterized by Fourier transform infrared spectroscopy, differential scanning calorimetry and scanning electron microscopy. Further these chelating hydrogels were investigated for the removal of rare earth metal ions (samarium and terbium) from aqueous environments by varying the adsorption time, adsorbate concentration and monomer ratio. The adsorption time data for samarium and terbium were fitted to two simple kinetic models, pseudo-first and pseudo-second-order, and tested to examine the adsorption mechanisms. The kinetic parameters were calculated. The equilibrium data was fitted well to the Langmuir and Freundlich models.
Muthaiya, Maria John,Nagella, Praveen,Thiruvengadam, Muthu,Mandal, AbulKalam Azad 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.2
Tea cell suspension culture is an alternative method for the synthesis of secondary metabolites. For the separation of cells, different concentrations of pectinase were used and a concentration of 0.5% was found to be the optimum concentration for the separation of cells (41.7%) in the culture medium than the other two concentrations (33.3 and 25.0%). The separated cells were cultured in liquid MS medium using different PGR combinations. The time taken for the cells to reach stationary phase, under different PGRs, ranged from 17 to 21 d. The maximum cell density was found in IAA and 2, 4-D medium at 21 d followed by 2, 4-D. Results revealed that the amount of secondary metabolites such as catechins were high with stationary phase when compared to other growth phases (lag and log phases). Different concentrations of shikimic acid (10, 20, and 30 mM) were added to the stationary phase of cell culture in the bioreactor and the secondary metabolite content was analyzed. Synthesis of polyphenols, catechins, caffeine, and other secondary components were high (33.87, 22.85, and 4.66%) with 20 mM shikimic acid treatment than the other two concentrations.
Suresh, Sekar,Prithiviraj, Ealumali,Venkatalakshmi, Nagella,Ganesh, Mohanraj Karthik,Ganesh, Lakshmanan,Lee, Hyun-Jeong,Prakash, Seppan The Korean Society of Animal Reproduction 2011 Reproductive & developmental biology Vol.35 No.4
The study aim is to investigate the free radicals scavenging and spermatogenic potentials, as well as to analyze any reproductive toxicity of ethanolic extract of Mucuna prureins (M. pruriens) Linn. in spermatozoa, under different dosages in normal male rat. Normal rats were randomly selected and suspension of the extract was administered orally at the dosages of 150, 200 and 250 mg/kg body weight of the different groups of male rats (n=6) once in a day for 60 days and grouped as group II, III and IV respectively. Saline treated rats served as control -group I. On the $60^{th}$ day the animals were sacrificed and the epididymal sperm were subjected to various analyses like level of ROS production, LPO, enzymatic and non enzymatic antioxidant, morphology, morphometry, chromosomal integrity and DNA damage. Results showed significant reduction in ROS production and peroxidation and significant increase in both enzymic and non-enzymic antioxidants in all concentration treated groups when compared with control. Results from all the drug treated groups showed good sperm morphology, increased sperm count and motility. There was no DNA damage and showed normal chromosomal integrity even in 250 mg/kg dose. When compared with control all the three extract treated groups showed increased ROS scavenging activity. However, group II (200 mg/kg) showed significant changes in all the parameters. From the present study it was confirmed that the M. pruriens has potential to improve the sperm qualitatively and quantitatively through scavenging the excess ROS with any adverse side effects. These observations suggest that ethanolic seed extract of M. pruriens may serve as anti-oxidant that can exploit to treat the oxidative stress mediated male factor infertility.