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Micropropagation from root segments to improve seedling quality in Chinese foxglove crops
Thanh Loan Pham,Van Huy Nguyen,Thi Le Thu Hoang,Thi Tam Tien Ha,Trung Kien Tran,Xuan Duong Vu,Phi Bang Cao,Quang Trung Nguyen 한국식물생명공학회 2020 JOURNAL OF PLANT BIOTECHNOLOGY Vol.47 No.3
This is the first study to establish a complete protocol for micropropagation of Rehmannia glutinosa from root segments. The study involved investigating the effect of plant growth regulators on in vitro shoot regeneration and rooting and identifying substrates supporting survival and growth performance of ex vitro seedlings. A Murashige and Skoog (MS) medium containing 30 g/L sucrose for shoot induction and 0.2 mg/L indole-3-acetic acid (IAA), 1 mg/L 6-benzylaminopurine (BAP), and 1 g/L polyvinylpyrrolidone (PVP) for shoot multiplication resulted in the highest number of shoots per explant and shoot height. Applying a medium containing 0.5 mg/L IAA and 1 g/L PVP yielded optimal rooting of the shoots grown in vitro. Compost enriched with microbial inoculants and perlite enhanced seedling growth better than that with organic biofertilizer-free substrates (soil and sand). We recommend the continuous production of micropropagated R. glutinosa seedlings from root segments under the aforementioned conditions as a possible propagation technique for crops of this species.
Enhancing biomass and lipid productivity of a marine microalga tetraselmis sp. With glycerol
( Dang Thanh Loan ),김필한,박한울,이철균 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0
Microalgae are one of the promising feedstocks for biodiesel production.During biodiesel production process from microalgae, glycerol is produced as a by-product from transesterification. As biodiesel production expands, global production of glycerol will continue to increase as well,which would reduce the price of glycerol to the point when selling glycerol is not profitable. In such case, utilization of glycerol for improved microalgal biomass and fatty acid production could be a solution. In this study, glycerol was added as a supplement to cultures of a marine microalga, Tetraselmis sp. KCT12432BP to investigate its effects on algal biomass and fatty acid production, Addition of glycerol improved biomass and fatty acid productivities by 36% and 56%, respectively. In the present study, microalgae could not consume all of the glycerol provided, and as the further study, the proper amount of glycerol supplement for efficient carbon utilization and improving biomass and fatty acids productivities will be investigated.
Using glycerol in mixotrophic culture of tetraselmis sp. To increase lipid productivity
( Dang Thanh Loan ),박한울,이철균 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0
Some microalgae can grow in mixotrophic condition with higher growth rate and lipid productivity compared with photoautotrophic condition. However, mixotrophic cultures are often not cost effective. In biodiesel production via transesterification, glycerol is produced along with fatty acid methyl ester. Using glycerol from transesterification for microalgae cultivation could be a way to reduce cultivation cost. In the present study, the effect of glycerol addition on the algal biomass and fatty acids productivity in Tetraselmis sp. MBEyh04Gc, was investigated. Addition of glycerol increased biomass and lipid productivity by 36% and 56%, respectively, compared with photoautotrophic cultures. The results suggest that glycerol can be used as a supplement in the culture of Tetraselmis sp., but further studies on the effects of quality and quantity of glycerol need to be conducted for optimization of mixotrophic cultivation.
Nguyen, Loan Thi Thanh,Jang, Ja Yeong,Kim, Tae Yoon,Yu, Nan Hee,Park, Ae Ran,Lee, Seungki,Bae, Chang-Hwan,Yeo, Joo Hong,Hur, Jae-Seoun,Park, Hae Woong,Kim, Jin-Cheol Elsevier 2018 Pesticide biochemistry and physiology Vol.148 No.-
<P><B>Abstract</B></P> <P> <UL> <LI> The widespread use of synthetic nematicides has caused significant problems to the environment as well as human health. To address this issue, eco-friendly control measures, such as microbial nematicides, are being developed. During the screening of <I>Myrothecium</I> strains with nematicidal activity against the root-knot nematode (RKN) <I>Meloidogyne incognita</I>, we found that the acetone extract of <I>Myrothecium</I> sp. KACC 40321 was highly effective against hatched juveniles of <I>M. incognita</I> at 7 days after exposure. The fungus was identified as <I>Meloidogyne verrucaria</I>. Two macrocyclic trichothecenes verrucarin A and roridin A were isolated and identified as major active metabolites by bioassay-guided fractionation and instrumental analysis. When the second-stage juveniles were treated with the chemicals, no juvenile mortality was observed. However, they effectively killed juveniles from treated eggs. The hatched juvenile mortality was used to evaluate the <I>in vitro</I> nematicidal activity of the compounds against <I>M. incognita</I>. The median effective concentrations were 1.88 μg/mL for verrucarin A and 1.50 μg/mL for roridin A. Among various liquid media, commercial malt extract broth (cMEB) was found to be the best for the production of verrucarin A and roridin A, followed by potato dextrose broth. The cMEB culture filtrate effectively reduced the formation of galls and egg masses on tomato roots in a pot experiment. In addition, the culture filtrate reduced the formation of galls on the roots of melon plants and the number of RKNs in the soils under field conditions. These results suggest that <I>M. verrucaria</I> KACC 40321 can be used as a biocontrol agent against RKNs in various crops. To the best of our knowledge, this is the first study to report the effectiveness of verrucarin A and rorridin A against hatched juveniles of <I>M. incognita</I>. </LI> </UL> </P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Myrothecium verrucaria</I> KACC 40321 was highly effective against hatched juveniles of the root-knot nematode (RKN) <I>Meloidogyne incognita.</I> </LI> <LI> Verrucarin A and roridin A were isolated from <I>M. verrucaria</I> KACC 40321 as active metabolites. </LI> <LI> The fungal culture filtrate effectively reduced the formation of galls and egg masses on roots of tomato and melon plants. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
In-situ Removal of Nitrogen and Phosphorus for the Prevention of Harmful Algal Blooms in River
( Dang Thanh Loan ),이철균 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.1
Harmful algal blooms (HABs) due to water eutrophication are becoming more serious in Korean rivers. There are many attempts to prevent HABs and the most effective method is to reduce nitrogen and phosphorus load from point and non-point source. However, this method is hard to apply in large area and is very expensive. On the other hand, microalgae have been utilized in wastewater treatment to reduce nitrogen and phosphorus level. In this study, ability to remove nitrogen and phosphorus in artificial river water by Euglena gracilis in permeable reactor was investigated. Permeable reactor keeps microalgae cells inside while let nutrients in artificial river water diffuse in for the growth of Euglena. The highest growth rate of Euglena in permeable reactor is 1 d-1at day 3. It is concluded that cultivating microalgae in permeable reactor could be an effective method to reduce nitrogen and phosphorus levels in river water.