Bioinformatic prediction and analysis of glucolipid metabolic regulation by miR-34a in Megalobrama amblycephala

Ling‑Hong Miao,Wen‑Jing Pan,Yan Lin,Bo Liu,Ming‑Chun Ren,Qun‑Lan Zhou,Xian‑Ping Ge 한국유전학회 2017 Genes & Genomics Vol.39 No.12

The objective of this study was to analyze the target genes and regulatory function of miR-34a in Megalobrama amblycephala using second-generation highthroughput sequencing and bioinformatic tools. Functional enrichment analysis was performed by gene ontology. MiR- 34a and target gene expression levels were measured in M. amblycephala fed normal and high-carbohydrate diets. The results revealed that miR-34a was highly conserved in several species, and miR-34a of M. amblycephala has a close evolutionary relationship to that of zebrafish and common carp. miRanda, TargetScan, RNAhybrid predicted 5,185, 6,282 and 2,168 target genes, respectively, and 645 target genes were in common. According to annotation information, the target genes were enriched in phosphate metabolism, glycerophospholipid metabolism, Golgi vesicle transport, cell division, and other biological processes (P < 0.05). Pathway enrichment analysis revealed that these target genes were mainly enriched in alpha-linolenic acid and linoleic acid metabolism, ether lipid metabolism, VEGF signaling pathway, Fc epsilon RI signaling pathway, GnRH signaling pathway, and MAPK signaling pathway (P < 0.05). The regulatory role of miR-34a was more significant in the liver than in the brain of M. amblycephala. MiR-34a regulates glucose lipid homeostasis induced by high glucose diets by upregulating hepatic PI3K/Akt, FOXO, and TOR signaling pathways.

Hypoxia Mediates Runt-Related Transcription Factor 2 Expression via Induction of Vascular Endothelial Growth Factor in Periodontal Ligament Stem Cells

Manjing Deng,Qian Xu,Zhihua Liu1,Ling Guo,Rui Liu,Rulei Li,Xiang Chu,Jiajia Yang,Jia Luo,Faming Chen 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.11

Periodontitis is characterized by the loss of periodontal tissues, especially alveolar bone. Common therapies cannot satisfactorily recover lost alveolar bone. Periodontal ligament stem cells (PDLSCs) possess the capacity of self-renewal and multilineage differentiation and are likely to recover lost alveolar bone. In addition, periodontitis is accompanied by hypoxia, and hypoxia-inducible factor-1α (HIF-1α) is a master transcription factor in the response to hypoxia. Thus, we aimed to ascertain how hypoxia affects runt-related transcription factor 2 (RUNX2), a key osteogenic marker, in the osteogenesis of PDLSCs. In this study, we found that hypoxia enhanced the protein expression of HIF-1α, vascular endothelial growth factor (VEGF), and RUNX2 ex vivo and in situ. VEGF is a target gene of HIF-1α, and the increased expression of VEGF and RUNX2 proteins was enhanced by cobalt chloride (CoCl2, 100 μmol/L), an agonist of HIF-1α, and suppressed by 3-(5′-hydroxymethyl-2′-furyl)-1-benzyl indazole (YC-1, 10 μmol/L), an antagonist of HIF-1α. In addition, VEGF could regulate the expression of RUNX2, as RUNX2 expression was enhanced by human VEGF (hVEGF165) and suppressed by VEGF siRNA. In addition, knocking down VEGF could decrease the expression of osteogenesis-related genes, i.e., RUNX2, alkaline phosphatase (ALP), and type I collagen (COL1), and hypoxia could enhance the expression of ALP, COL1, and osteocalcin (OCN) in the early stage of osteogenesis of PDLSCs. Taken together, our results showed that hypoxia could mediate the expression of RUNX2 in PDLSCs via HIF-1α-induced VEGF and play a positive role in the early stage of osteogenesis of PDLSCs

A Robust Method for Automatic Segmentation and Recognition of Apoptosis Cell

Liu Hai-Ling(류해릉),Young-Suk Shin(신영숙) 한국정보과학회 2009 정보과학회 컴퓨팅의 실제 논문지 Vol.15 No.6

본 연구는 Apoptosis세포들의 형상을 검출하기 위하여 전통적인 세포측정법과는 다른 영상기반 접근법을 제안한다. 이 방법은 세포측정법의 단점을 극복하고 Apoptosis세포들을 정확하게 인식할 수 있다. 본 연구에서 K-means 군집화 방법이 Apoptosis 세포의 거시적인 분할을 행하는 데 사용되었으며, ‘스네이크’라고 불리는 액티브윤곽선 모델이 정밀한 경계선 검출을 위해 사용되었다. 그리고 Apoptosis세포들의 물리적 특징, 형태적 특징 그리고 무늬특징들을 포함하는 몇가지 특징들이 추출되었다. 마지막으로 Mahalanobis 거리 분류기가 Apoptosis세포와 비Apoptosis 세포로서 분할영상들을 분류한다. In this paper we propose an image-based approach, which is different from the traditional flow cytometric method to detect shape of apoptosis cells. This method can overcome the defects of cytometry and give precise recognition of apoptosis cells. In this work K-means clustering was used to do the rough segmentation and an active contour model, called ‘snake’ was used to do the precise edge detection. And then some features were extracted including physical feature, shape descriptor and texture features of the apoptosis cells. Finally a Mahalanobis distance classifier classifies the segmentation images as apoptosis and non-apoptosis cell.

Evolution of Tensile Properties of Compacted Red Clay under Wet and Dry Cycles

Ling Zeng,Hui-Cong Yu,Qian-Feng Gao,Jie Liu,Zi-Han Liu 대한토목학회 2022 KSCE Journal of Civil Engineering Vol.26 No.2

Tensile strength is an important soil parameter that affects the stability of structures built on clayey soils. This paper presents an experimental study of the change of tensile properties of red clay during wet-dry cycles. Cyclic wet-dry tests were performed on compacted red clay specimens with different initial water contents and dry densities. Direct tensile tests and Brazilian split tests were then conducted on these specimens to determine the soil strengths. The effects of initial water content, initial dry density, number of wet-dry cycles, and crack rate on the tensile properties of red clay were investigated. The results demonstrate that the tensile strength of red clay is generally 1.566 times the splitting strength. Both the tensile strength and splitting strength are negatively correlated with the initial water content but are positively correlated with the initial dry density. Because of the presence of desiccation cracks, the tensile strength goes on reducing under wet-dry cycles. The tensile strength can be expressed by a power function of the initial water content, initial dry density, and crack rate. The proposed equation is useful to evaluate the tensile strength of cracked soils subjected to wet-dry cycles.

The Components of Cacalia tangutica

Liu, Zhen-Ling,Tian, Xuan Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.7

Effect of plant polyphenols on the formation of advanced glycation end products from β-lactoglobulin

Ling Liu,Rikke V. Hedegaard,Leif H. Skibsted 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.2

Dietary exposure to advanced glycation end products (AGEs) formed from proteins and reducing sugars is of increasing concern to human health. AGEs may form in protein-based powders containing sugars for instant beverages during drying and storage of the product. Chlorogenic acid, a plant phenol characteristic of coffee, was found to protect against the formation of AGEs at a concentration of 50mM during heating of β-lactoglobulin in the presence of glucose as a reducing sugar in 30% aqueous ethanol at 70oC. Epicatechin, a plant phenol characteristic of green tea, had no similar effect for the equivalent concentration of phenol on the formation of AGEs. Immunochemical detection (ELISA) using polyclonal antibodies raised against AGEs showed a dose-dependent effect of protection by chlorogenic acid on AGE formation and is recommended for routine quality control of sugar containing milk-based powders for instant beverages.

Monosized core-shell Fe3O4(Fe)/Au multifunctional nanocrystals.

Liu, Hong-Ling,Wu, Jun-Hua,Min, Ji Hyun,Lee, Ju Hun,Kim, Young Keun American Scientific Publishers 2009 Journal of Nanoscience and Nanotechnology Vol.9 No.2

<P>We have synthesized highly crystalline, monosized Fe3O4(Fe)/Au magnetic-optic multifunctional nanocrystals using a modified polyol process. The formation of the core-shell structure was achieved in two consecutive steps, seeding the Fe3O4(Fe) core followed by coating the gold shell. The characterization demonstrates the core-shell nanostructure and provides particle size and distribution of the nanocrystals. The spectroscopic analysis gives three Fe oxidation states (Fe0, Fe+2 and Fe+3) in the core and the surface plasmon characteristic of the Au nanoshell, while the magnetic measurements show excellent susceptibility of the nanocrystals.</P>

Efficient Compression Algorithm with Limited Resource for Continuous Surveillance

( Ling Yin ),( Chuanren Liu ),( Xinjiang Lu ),( Jiafeng Chen ),( Caixing Liu ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.11

Energy efficiency of resource-constrained wireless sensor networks is critical in applications such as real-time monitoring/surveillance. To improve the energy efficiency and reduce the energy consumption, the time series data can be compressed before transmission. However, most of the compression algorithms for time series data were developed only for single variate scenarios, while in practice there are often multiple sensor nodes in one application and the collected data is actually multivariate time series. In this paper, we propose to compress the time series data by the Lasso (least absolute shrinkage and selection operator) approximation. We show that, our approach can be naturally extended for compressing the multivariate time series data. Our extension is novel since it constructs an optimal projection of the original multivariates where the best energy efficiency can be realized. The two algorithms are named by ULasso (Univariate Lasso) and MLasso (Multivariate Lasso), for which we also provide practical guidance for parameter selection. Finally, empirically evaluation is implemented with several publicly available real-world data sets from different application domains. We quantify the algorithm performance by measuring the approximation error, compression ratio, and computation complexity. The results show that ULasso and MLasso are superior to or at least equivalent to compression performance of LTC and PLAMlis. Particularly, MLasso can significantly reduce the smooth multivariate time series data, without breaking the major trends and important changes of the sensor network system.

Fabrication of CeO2@MnO2 Core–Shell Nanospheres and Their Application in CO Oxidation

Ling Liu,Jingjing Shi,Hong-Xia Cao,Ruiyu Wang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.3

Rational design and fabrication of heterogeneous core–shell nanostructures is of great significance because their two-phase interface could render hybrid junctions with rich redox reactions, which are beneficial for improving catalytic performance. Herein, hierarchical CeO2 yolk-shell nanospheres@MnO2 nanosheets core–shell nanostructures were fabricated by a multistep wet chemical synthetic strategy. The resulting CeO2@MnO2 core–shell nanospheres exhibited high catalytic activity toward CO oxidation in comparison with pure CeO2 and MnO2 structures and their physical and chemical mixture. The enhanced catalytic performance should be attributed to the formation of core–shell structure and the possible synergistic effects between the two components of MnO2 and CeO2, which facilitates materials with promoted redox properties and enhanced oxidation activity. In addition, this synthetic method is expected to extend to fabricate other multi-composite core–shell structures for a broad range of technical applications.

Reading and Writing Our Own Stories : The Alternative Reading Group

LIU, Kai-ling Asian Center Women's Studies Korean Women's Instit 2002 Asian Journal of Women's Studies(AJWS) Vol.8 No.2

Since women are the primary participants in most reading groups in Taiwan, this research experiments with the use of women's personal narratives in one such group. Reading of ordinary women's personal narratives encourages women to reflect on their own lives and then write out their own life stories. Further, with the sense of community that this reading and writing generates, women may come to a better recognition of their own value. This group, comprising ten middle-aged women, held two-hour meetings once a week for twenty weeks. In the reading phase, the participants read 7 publications of women's personal narratives, which came in various forms such as letters, diaries, autobiographies, oral testimonies, and biographies. In the writing phase, the participants wrote their life stories, dwelling on themes of family, education, women and the body, the family-in-law, care-giving, appearance, and miscellany. This exercise led to three kinds of results. First, the reading of ordinary women's personal narratives introduced participants with the form and content of writing life stories. Then, the writing of their own life stories enabled the women participants to re-evaluate their past. Third, with the reading, writing, and group sharing, a collection of writing or what I call a `community of narratives' was formed. This helped the participants recognize the differences amongst them and at the same time to value their own experience.