Ischemic postconditioning protects cardiomyocytes against ischemia/reperfusion injury by inducing MIP2

Hong-Lin Zhu,Kang-Kai Wang,Xing Wei,Shun-Lin Qu,Chi Zhang,Xiao-Xia Zuo,Yan-Sheng Feng,Qi Luo,Guang-Wen Chen,Mei-Dong Liu,Lei Jiang,Xian-Zhong Xiao 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.8

Cardiomyocytes can resist ischemia/reperfusion (I/R)injury through ischemic postconditioning (IPoC)which is repetitive ischemia induced during the onset of reperfusion. Myocardial ischemic preconditioning up-regulated protein 2 (MIP2) is a member of the WD-40family proteins, we previously showed that MIP2 was up-regulated during ischemic preconditioning (IPC). As IPC and IPoC engaged similar molecular mechanisms in cardioprotection, this study aimed to elucidate whether MIP2 was up-regulated during IPoC and contributed to IPoC-mediated protection against I/R injury. The experiment was conducted on two models,an in vivo open chest rat coronary artery occlusion model and an in vitro model with H9c2 myogenic cells. In both models, 3 groups were constituted and randomly designated as the sham, I/R and IPoC/hypoxia postconditioning (HPoC) groups. In the IPoC group, after 45 min of ischemia, hearts were allowed three cycles of reperfusion/ischemia phases (each of 30 s duration)followed by reperfusion. In the HPoC group, after 6 h of hypoxia, H9c2 cells were subjected to three cycles of 10 minute reoxygenation and 10 minute hypoxia followed by reoxygenation. IPoC significantly reduced the infarct size, plasma level of Lactate dehydrogenase and creatine kinase MB in rats. 12 h after the reperfusion,MIP2 mRNA levels in the IPoC group were 10 folds that of the sham group and 1.4 folds that of the I/R group. Increased expression of MIP2 mRNA and attenuation of apoptosis were similarly observed in the HPoC group in the in vitro model. These effects were blunted by transfection with MIP2 siRNA in the H9c2cells. This study demonstrated that IPoC induced protection was associated with increased expression of MIP2. Both MIP2 overexpression and MIP2 suppression can influence the IPoC induced protection.

Simulation analysis and evaluation of decontamination effect of different abrasive jet process parameters on radioactively contaminated metal

Zhong Lin,Deng Jian,Zuo Zhe-wen,Huang Can-yu,Chen Bo,Lei Lin,Lei Ze-yong,Lei Jie-heng,Zhao Mu,Hua Yun-fei 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.11

A new method of numerical simulating prediction and decontamination effect evaluation for abrasive jet decontamination to radioactively contaminated metal is proposed. Based on the Computational Fluid Dynamics and Discrete Element Model (CFD-DEM) coupled simulation model, the motion patterns and distribution of abrasives can be predicted, and the decontamination effect can be evaluated by image processing and recognition technology. The impact of three key parameters (impact distance, inlet pressure, abrasive mass flow rate) on the decontamination effect is revealed. Moreover, here are experiments of reliability verification to decontamination effect and numerical simulation methods that has been conducted. The results show that: 60Co and other homogeneous solid solution radioactive pollutants can be removed by abrasive jet, and the average removal rate of Co exceeds 80%. It is reliable for the proposed numerical simulation and evaluation method because of the well goodness of fit between predicted value and actual values: The predicted values and actual values of the abrasive distribution diameter are Ф57 and Ф55; the total coverage rate is 26.42% and 23.50%; the average impact velocity is 81.73 m/s and 78.00 m/s. Further analysis shows that the impact distance has a significant impact on the distribution of abrasive particles on the target surface, the coverage rate of the core area increases at first, and then decreases with the increase of the impact distance of the nozzle, which reach a maximum of 14.44% at 300 mm. It is recommended to set the impact distance around 300 mm, because at this time the core area coverage of the abrasive is the largest and the impact velocity is stable at the highest speed of 81.94 m/s. The impact of the nozzle inlet pressure on the decontamination effect mainly affects the impact kinetic energy of the abrasive and has little impact on the distribution. The greater the inlet pressure, the greater the impact kinetic energy, and the stronger the decontamination ability of the abrasive. But in return, the energy consumption is higher, too. For the decontamination of radioactively contaminated metals, it is recommended to set the inlet pressure of the nozzle at around 0.6 MPa. Because most of the Co elements can be removed under this pressure. Increasing the mass and flow of abrasives appropriately can enhance the decontamination effectiveness. The total mass of abrasives per unit decontamination area is suggested to be 50 g because the core area coverage rate of the abrasive is relatively large under this condition; and the nozzle wear extent is acceptable

Ischemic postconditioning protects cardiomyocytes against ischemia/reperfusion injury by inducing MIP2

Zhu, Hong-Lin,Wei, Xing,Qu, Shun-Lin,Zhang, Chi,Zuo, Xiao-Xia,Feng, Yan-Sheng,Luo, Qi,Chen, Guang-Wen,Liu, Mei-Dong,Jiang, Lei,Xiao, Xian-Zhong,Wang, Kang-Kai Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.8

Cardiomyocytes can resist ischemia/reperfusion(I/R) injury through ischemic postconditioning (IPoC) which is repetitive ischemia induced during the onset of reperfusion. Myocardial ischemic preconditioning up-regulated protein 2 (MIP2) is a member of the WD-40 family proteins, we previously showed that MIP2 was up-regulated during ischemic preconditioning (IPC). As IPC and IPoC engaged similar molecular mechanisms in cardioprotection, this study aimed to elucidate whether MIP2 was up-regulated during IPoC and contributed to IPoC-mediated protection against I/R injury. The experiment was conducted on two models, an $in$ $vivo$ open chest rat coronary artery occlusion model and an $in$ $vitro$ model with H9c2 myogenic cells. In both models, 3 groups were constituted and randomly designated as the sham, I/R and IPoC/hypoxia postconditioning (HPoC) groups. In the IPoC group, after 45 min of ischemia, hearts were allowed three cycles of reperfusion/ischemia phases (each of 30 s duration) followed by reperfusion. In the HPoC group, after 6 h of hypoxia, H9c2 cells were subjected to three cycles of 10 minute reoxygenation and 10 minute hypoxia followed by reoxygenation. IPoC significantly reduced the infarct size, plasma level of Lactate dehydrogenase and creatine kinase MB in rats. 12 h after the reperfusion, MIP2 mRNA levels in the IPoC group were 10 folds that of the sham group and 1.4 folds that of the I/R group. Increased expression of MIP2 mRNA and attenuation of apoptosis were similarly observed in the HPoC group in the $in$ $vitro$ model. These effects were blunted by transfection with MIP2 siRNA in the H9c2 cells. This study demonstrated that IPoC induced protection was associated with increased expression of MIP2. Both MIP2 overexpression and MIP2 suppression can influence the IPoC induced protection.

The Square Root Cubature Kalman Filter-Markov Ahead Estimation Based Tracking Maneuvering Photoelectric Target Systems

Tao Zuo,Huasong Min,Yunhan Lin,Shaowei Wang 대한전기학회 2019 Journal of Electrical Engineering & Technology Vol.14 No.4

A new method of ahead estimated tracking maneuvering targets is proposed to overcome the inherent measurement delay and control hysteresis. Square Root Cubature Kalman Filter-Markov (SRCKF-Markov) ahead estimation is used to predict the maneuvering target states at the next sampling time in advance. The “current” statistical models of maneuvering targets are set up, and the maneuvering states at the next sampling time are one-step ahead estimated by SRCKF. Then, the Markov matrixes of transition probability are set up with the errors between the prior estimates by SRCKF and the measured values. The next error between the prior estimate by SRCKF and the measured value is one-step ahead predicted by the matrixes of transition probability. The estimates of maneuvering target states with SRCKF are revised by the estimates of next errors. Meanwhile, a self-correcting predictive controller is used in the control system to overcome the control hysteresis. Simulations and experiment results show that the proposed estimation and control method can improve the accuracy of ahead estimated tracking greatly.

MicroRNAs: their involvement in fibrosis pathogenesis and use as diagnostic biomarkers in scleroderma

Hong-Lin Zhu,Hui Luo,Xiao-Xia Zuo 생화학분자생물학회 2013 Experimental and molecular medicine Vol.45 No.9

miRNAs are important post-transcriptional regulators. The aberrant expression of miRNAs is strongly associated with the initiation and progression of pathophysiologic processes in a wide range of human diseases. Scleroderma (systemic sclerosis;SSc) is a highly heterogeneous autoimmune disease that includes the progressive fibrotic replacement of normal tissue architecture in multiple organs. Our previous studies have suggested that SSc skin tissues display a different miRNA expression signature than that found in normal controls. miRNAs with pro- or antifibrotic properties are found to be dysregulated in SSc skin fibrosis. Serum miRNA levels are associated with SSc activity and severity. miRNAs have the potential to be therapeutic targets and serve as biomarkers for SSc diagnosis and assessment of disease state and severity. This review summarizes the SSc miRNA expression signature and the roles of dysregulation of miRNAs in SSc tissues and serum and examines the future therapeutic potential of targeting miRNAs in the management of SSc patients.

Sex difference of autosomal alleles in populations of European and African descent

Lingjun Zuo,Tong Wang,Xiandong Lin,Jijun Wang,Yunlong Tan,Xiaoping Wang,Xueqing Yu,Xingguang Luo 한국유전학회 2015 Genes & Genomics Vol.37 No.12

In the present study, we aimed to report the individual sex-different genetic markers across autosomes in European- and African-origin populations. A total of 8400 females and 8081 males in 19 independent cohorts were genotyped across genomes using Illumina or Affymetrix arrays. The allele frequencies were compared between females and males in nine non-clean cohorts (with some human disease traits) using genome-wide logistic regression and then the nominally significant associations were replicated across 10 clean cohorts (without disease traits). Meta-analysis was performed to derive the combined p values across all cohorts. We found 13 markers that were genome-wide significant (p B 5 9 10-8) between females and males in the meta-analysis of all cohorts of European descent, including rs7740449 at SYNE1, rs7531151 at PLD5, rs697455 at PPP1R12B, rs6745746 at LOC100128413, rs17000079 at PARM1, rs11948070 at PDE4D, rs7801825 at INSIG1, rs9551642 at MTUS2, rs2932174 at TPTE2, rs1961597 at SALL3, rs4117529 at METTL4, rs6021473 at SALL4 and rs6092466 at RAE1, and one marker, i.e., rs10145208 at PCNX, that was genomewide significant in the meta-analysis of all cohorts of African descent. The most robust finding was rs7740449 at SYNE1, next to ESR1. We conclude that there are many sex-different markers on autosomes. These markers may be informative in differentiating females and males.

A Three-way Handshaking Access Mechanism for Point to Multipoint In-band Full-duplex Wireless Networks

( Haiwei Zuo ),( Yanjing Sun ),( Changlin Lin ),( Song Li ),( Hongli Xu ),( Zefu Tan ),( Yanfen Wang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.7

In-band Full-duplex (IBFD) wireless communication allows improved throughput for wireless networks. The current Half-duplex (HD) medium access mechanism Request to Send/Clear to Send (RTS/CTS) has been directly applied to IBFD wireless networks. However, this is only able to support a symmetric dual link, and does not provide the full advantages of IBFD. To increase network throughput in a superior way to the HD mechanism, a novel three-way handshaking access mechanism RTS/SRTS (Second Request to Send)/CTS is proposed for point to multipoint (PMP) IBFD wireless networks, which can support both symmetric dual link and asymmetric dual link communication. In this approach, IBFD wireless communication only requires one channel access for two-way simultaneous packet transmissions. We first describe the RTS/SRTS/CTS mechanism and the symmetric/asymmetric dual link transmission procedure and then provide a theoretical analysis of network throughput and delay using a Markov model. Using simulations, we demonstrate that the RTS/SRTS/CTS access mechanism shows improved performance relative to that of the RTS/CTS HD access mechanism.

Numerical investigation on gypsum and ettringite formation in cement pastes subjected to sulfate attack

Xiao-Bao Zuo,Jia-Lin Wang,Wei Sun,Hua Li,Guang-Ji Yin 사단법인 한국계산역학회 2017 Computers and Concrete, An International Journal Vol.19 No.1

This paper uses modelling and experiment to perform a quantitative analysis for the gypsum and ettringite formations in cement pastes subjected to sulfate attack. Firstly, based on Fick’s law and chemical reaction kinetics, a diffusion model of sulfate ions in cement pastes is proposed, and then the model of the gypsum and ettringite formations is established to analyze its contents in cement pastes with corrosion time. Secondly, the corrosion experiment of the specimens with cement pastes immersed into 2.5%, 5.0% and 10.0% Na2SO4 solutions are carried out, and by using XRD-Rietveld method, the phases of powder samples from the specimens are quantitatively analyzed to obtain the contents of gypsum and ettringite in different surface depth, solution concentration and corrosion time. Finally, the contents of gypsum and ettringite calculated by the models are compared with the results from the XRD experiments, and then the effects of surface depth, corrosion time and solution concentration on the gypsum and ettringite formations in cement pastes are discussed.

Photoactivation and inactivation of <i>Arabidopsis</i> cryptochrome 2

Wang, Qin,Zuo, Zecheng,Wang, Xu,Gu, Lianfeng,Yoshizumi, Takeshi,Yang, Zhaohe,Yang, Liang,Liu, Qing,Liu, Wei,Han, Yun-Jeong,Kim, Jeong-Il,Liu, Bin,Wohlschlegel, James A.,Matsui, Minami,Oka, Yoshito,Lin American Association for the Advancement of Scienc 2016 Science Vol.354 No.6310

<P>Cryptochromes are blue-light receptors that regulate development and the circadian clock in plants and animals. We found that Arabidopsis cryptochrome 2 (CRY2) undergoes blue light-dependent homodimerization to become physiologically active. We identified BIC1 (blue-light inhibitor of cryptochromes 1) as an inhibitor of plant cryptochromes that binds to CRY2 to suppress the blue light-dependent dimerization, photobody formation, phosphorylation, degradation, and physiological activities of CRY2. We hypothesize that regulated dimerization governs homeostasis of the active cryptochromes in plants and other evolutionary lineages.</P>

ON REES MATRIX REPRESENTATIONS OF ABUNDANT SEMIGROUPS WITH ADEQUATE TRANSVERSALS

Gao, Zhen Lin,Liu, Xian Ge,Xiang, Yan Jun,Zuo, He Li Korean Mathematical Society 2009 대한수학회논문집 Vol.24 No.4

The concepts of *-relation of a ($\Gamma$-)semigroup and $\bar{\Gamma}$-adequate transversal of a ($\Gamma$-)abundant semigroup are defined in this note. Then we develop a matrix type theory for abundant semigroups. We give some equivalent conditions of a Rees matrix semigroup being abundant and some equivalent conditions of an abundant Rees matrix semigroup having an adequate transversal. Then we obtain some Rees matrix representations for abundant semigroups with adequate transversals by the above theories.