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      • Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps Sinensis in the Bronchoalveolar Lavage Fluids Cells

        Kuo, Yuh-Chi,Wu, Chia-Lin,Tasi, Wei-Jern,Wang, Jir-Yenn,Chang, Shi-Chung,Lin, Ching-Yuang,Shiao, Ming-Shi 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

        Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang₃,Ching-Yung Lin⁴,and Ming-Shi Shiao⁴¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang²,Ching-Yung Lin³,and Ming-Shi Shiao⁴.¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan. Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps sinensis in the Bronchoalveolar Lavage Fluids Cells. Proceedings of international Symposium on East-West Medicine, Seoul. 44-64, 1999.-Cordyceps sinensis is a major parasitic fungus on larva of Lepidoptera. It is one of the well know fungi used in traditional Chinese medicine for treatment asthma, lung and kideney diseases. Many fungi belonging to the genus Cordyceps have been demonstrated to produce natural products with various biological activities. Reported biological activities of genus cordyceps include:(a)inhibition of DNA and RNA synthesis;(b)enhancement of cell differentiation;(c)restruction of cytoskeleton;(d)inhibition of protein kinase activity;(e)antitumor activity on bladder, colon, lung carcinoma as well as fibroblastoma;(f)inhibition of the infection and revers transciptase activity of human immunodeficiency virus type 1; and(g) inhibition of methylation of nucleic acid. Thus, it is suggested that the extracts of Cordyceps sp. Contain antitumor, antiviral, and many other biological activities. In our previous studies, we proved that C. sinensis contains the antitumor and immunomodulatory agents. In the present studies, effects of C. sinensis on bronchoalveolar lavage fluids(BAL)cells were demonstrated. The crude methanolic extracts of C. sinensis were fractionated into 15 fractions by silica gel column chromatography. Effects of various fraction on BAL cells proliferation and lipopolysaccharide(LPS) stimulated interleukin-1β(IL-8),platelet activating factor(PAF), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α),and interleukin-8(IL-8) production on BAL cells were determined. The result indicated that the CS-19-22 fraction suppressed BAL cells proliferation activated by LPS. The median inhibitory concentration(IC50) was 6㎍/ml. The CS-19-22 fraction also decreased IL-1β,PAF,IL-6, TNF-αand IL-8 production.The results of revers transcription-polymerase chain reaction(RT-PCR) demonstrated that the CS-19-22 fraction did not affect IL-1β,PAF,IL-6, TNF-α,and IL-8 mRNAs expression in BAL cells activated by LPS. By contrast, it inhibited the IL-10 mRNA expression but enhanced IFN-γand IL-12 mRNAs expression in activated BAL cells. Moreover, the CS-19-22 fraction blocked PAF-induced platelet aggregation. It is unlikely that cytotoxicity was involved. Because no cell deths were observable. We hypothesize that inhibitory mechanisms of CS-19-22 on BAL cells proliferation may be related to the impairments of gene expression and production of cytokines in BAL cells. The explanation of C. sinensis antiasthma function may involved the following(1)C.sinenesis contained immunomodulatory agents that stimulated IFN-γand IL-12 expression in TH1cells.(2)Enhanced secretion of IFN-γand IL-12 will inhibit the Tγcells immune responses Ex. Decreasing of IL-6 and IL-10 production.(3) The impairments of cytokines production in TH2 cells suppressed th BAL cells proliferation then more cytokines production Ex.IL-1β,PAF,IL-6,TNF-αand IL-8 in activated BAL cells were decreased. (4)The suppression of TH2cells immune responses will inhibit the growth and differentiation of B cells then IgE production will be decreased. (5)Reduced production of IgE will decreased asthma attack occurring in individuals. However, the results of the present study suggested that C. sinensis may also have acted to treat asthma in part by inhibiting BAL cells proliferation and cytokine gene expression and production. Future, experiments with treatment of animals with asthma with C. sinensis will be necessary to defined whether C. sinensis can reduce asthma injury. Plans are underway for the isolation of pure principal growth inhibitors and their mechanisms of action are subjected for further study.

      • 6면 프리즘 렌즈가 장착된 Lensmeter에서 시험 렌즈의 편심 위치 계산

        Maria Lin,박종대,,조창호,김현규 배재대학교 자연과학연구소 2005 自然科學論文集 Vol.16 No.1

        6면 프리즘 렌즈가 장착된 렌즈메터에서 상점의 위치로부터 시험렌즈의 편심의 위치를 알아 낼 수 있는 알고리즘을 개발하였다. 6개의 프리즘에 의해 형성되는 6개의 상점은 시험렌즈의 편심의 극좌표에 의해 변하게 되며, 비틀린 광선 추적으로 계산할 수 있다. 광학 설계 프로그램인 Code V를 사용하여 렌즈메터 광학계를 분석하고, 시험렌즈의 위치에 따른 상점의 위치 변화를 함수로 나타내서 역으로 상점의 위치로부터 시험렌즈의 편심의 위치를 알아낼 수 있는 방법을 개발하였다. We have developed an algorithm to determine the decenter position from the image points in a lensmeter with 6-sided prism lens. The positions of the image points which are formed by six prisms depends on the decenter position of a test lens and can be calculated by skew ray tracing. The optical characteristics of the lens meter was analyzed using the Code V program and the positions of the image points was expressed as a function of the decenter position of the test lens. By minimizing the expectation error, we can determine the decenter position of a test lens from the image positions.

      • SCIESCOPUSKCI등재

        Reversible Data Hiding Scheme for VQ Indices Based on Absolute Difference Trees

        Chang, Chin-Chen,Nguyen, Thai-Son,Lin, Chia-Chen Korean Society for Internet Information 2014 KSII Transactions on Internet and Information Syst Vol.8 No.7

        Reversible data hiding is a technique for recovering original images without any distortion after secret data are extracted from the image. The technique continues to attract attention from many researchers. In this paper, we introduce a new reversible data hiding scheme based on the adjacent index differences of vector quantization (VQ) indices. The proposed scheme exploits the differences between two adjacent indices to embed secret data. Experimental results show that our scheme can achieve a lower compression rate than an earlier scheme by Yang and Lin. Our scheme's average compression rate, 0.44 bpp, outperforms that of Yang and Lin's scheme, which averages 0.53 bpp. Moreover, the embedding capacity of our scheme can rise to 1.45 bpi, which also is superior to that of Chang et al.'s scheme [35] (1.00 bpi)Yang and Lin's scheme [27] (0.91 bpi) as well as Chang et al.'s scheme [26] (0.74 bpi).

      • KCI등재

        Reversible Data Hiding Scheme for VQ Indices Based on Absolute Difference Trees

        ( Chin-chen Chang ),( Thai-son Nguyen ),( Chia-chen Lin ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.7

        Reversible data hiding is a technique for recovering original images without any distortion after secret data are extracted from the image. The technique continues to attract attention from many researchers. In this paper, we introduce a new reversible data hiding scheme based on the adjacent index differences of vector quantization (VQ) indices. The proposed scheme exploits the differences between two adjacent indices to embed secret data. Experimental results show that our scheme can achieve a lower compression rate than an earlier scheme by Yang and Lin. Our scheme`s average compression rate, 0.44 bpp, outperforms that of Yang and Lin`s scheme, which averages 0.53 bpp. Moreover, the embedding capacity of our scheme can rise to 1.45 bpi, which also is superior to that of Chang et al.`s scheme [35] (1.00 bpi)Yang and Lin`s scheme [27] (0.91 bpi) as well as Chang et al.`s scheme [26] (0.74 bpi).

      • KCI등재후보

        The Study about Diversification of the Visual Modeling Identification Method in the Digital Times

        Lin,Chen-hao 한국조형디자인학회 2013 조형디자인연구 Vol.16 No.4

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

        In this paper, a mobile application called 'Easy Logo' is proposed. It is able to detect the logo part from a picture captured by user using their mobile phone (iPhone) camera, recognize logo, send back results and display relevant logo name, information and link to websites on iPhone screen. This article compares different algorithm like SIFT, Gist and Hog to extract features and build classification model. It demonstrates the system flow, the technical algorithm and detailed experiment comparison and results.

      • Expression and Clinical Significance of Myeloid Derived Suppressor Cells in Chronic Hepatitis B Patients

        Lu, Li-Rong,Liu, Jing,Xu, Zhen,Zhang, Geng-Lin,Li, De-Chang,Lin, Chao-Shuang Asian Pacific Organization for Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10

        We here document discovery of expression profile of myeloid derived suppressor cells (MDSCs) in chronic hepatitis B (CHB) patients and changes in the course of disease. The study population was composed of 75 outpatient HBV cases and 15 healthy control cases. Peripheral blood samples were collected for separation of mononuclear cells. Levels of MDSCs labeled with Lin-DR-CD11b+CD33+ obtained from peripheral blood mononuclear cells (PBMC), were revealed to have significant differences between the CHB and other groups. They were 0.414% for health control cases and 0.226% for CHB cases (Z=-2.356, p=0.0189). It also observed that the group of HBeAg positive cases had significant difference in MDSCs/PBMC median ($X^2=11.877$, p=0.003), compared with group of HBeAg negative cases and the healthy control group. It suggested considerable MDSCs might be involved in HBeAg immune tolerance. In addition, negative correlations between MDSCs/PBMC and parameters of ALT, AST and TBil, while positive correlation between MDSCs/PBMC and ALB parameter were found. Multiple comparisons between the four phases and health control phase again, there was a statistically sifnificant difference ($X^2=17.198$, p=0.002). Taken together, these findings may provide a new immunotherapy strategy for reduced the expression levels of MDSCs in CHB patients, through induction of an autoimmune response to virus removal.

      • SCISCIESCOPUS
      • SCISCIESCOPUS

        Effect of influential factors on microbial growth and the correlation between current generation and biomass in an air cathode microbial fuel cell

        Li, N.,Kakarla, R.,Min, B. Pergamon Press ; Elsevier Science Ltd 2016 International journal of hydrogen energy Vol.41 No.45

        <P>Microbial biomass in a microbial fuel cell (MFC) was determined at varied external resistances and temperatures, and the current generations were correlated with biomass concentration. MFC operation with wastewater generated the highest current of 0.23 mA at 300 Omega compared with 100 Omega (0.22 mA) and 1000 Omega (0.18 mA). The microbial biomass (0.091 mg-protein/cm(2)) on the anode with 100 Omega was higher than the other resistances. With growth media, current and biomass density were increased due to high strength electrolyte and nutrient. The MFCs with higher current generation contained a more amount of biomass on an anode electrode. The highest biomass growth rate with growth media was 1.307 x 10(-4) mg-protein/hr.cm(2) at 30 degrees C (with 300 Omega), which was about 2 times higher than with wastewater. In linear regression, current generation is expected to increase by about 3 times as the biomass is doubled on the anode electrode. (C) 2016 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.</P>

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