Detection of Antibokies to Sendai Virus Using protein G-based ELISA

임윤규,우희종,이영순 濟州大學校 農科大學 動物科學硏究所 1993 動物科學論叢 Vol.8 No.1

We have established an enzyme-linked immunosorbent assay(EL1SA) using enzyme labeled protein G(Protein G-ELISA) to detect Sendai virus-specific antibodies in laboratory animals. Best results were obtained at fl 6.0 of diluent and at 1 to 10 dilution of sera. Antigen concen-tration for solid phase matrix was 5μg /㎖ and the conjugate of horseradish peroxidase (HRP) and prqtein G was used at 1 to 100 dilution. We, also, investigated the sensitivity and speci-ficity of protein G and protein A. Protein C showed relatively high O. D. values than protein A in general. Furthermore, only Protein G-ELISA could detect the Sendai virus specific anti-bodies in rat. though both methods were effective in other species, mouse and guinea pig. No siginificant nonspecific reation was observed at our experimental conditions in both methods.

시판 요구르트 Starter의 생리적 특성

임용숙,김순희,이신호 대구효성가톨릭대학교 응용과학연구소 1994 응용과학연구논문집 Vol.3 No.-

시판 호상 요구르트의 품질 검사와 분리된 유산균의 starter특성을 조사한 결과 시판 요구르트의 유산균 수는 9개 회사 시료중 1개 회사가 1.66×10^6CFU/g 을 나타내었고 그외 8개 회사제품은 10^8 ~ 10^9CFU/ml 범위를 나타내었다. 제품의 pH 범위는 4.00 ~ 4.33 으로 비교적 산미가 강하였으며, 적정산도는 1.063 ~ 1.321% 였다. 순수 분리한 요구르트 starter의 산 생성 속도는 배양 시간이 경과함에 따라 적정산도가 모두 증가하는 것으로 나타났다. 특히 배양 12시간째 starter B가 산 생성이 가장 좋았으며, starter E의 산 생성 속도가 가장 늦었다. 9개 회사 starter는 공히 위액과 담즙산에 강한 내성이 관찰되었으며 특히 D, E, G 균주의 경우 위액과 담즙산에 거의 생존하여 가장 내성이 강한 경향을 나타내었다. Viable cells, pH and titrable acidity of nine kinds of marketing gel-type yoghurts and acid production and survival in digestive fluids and bile salts of the isolated starters were investigated. Viable cells of one sample was 1.66×10^6(CFU/g) and other eight samples show-ed about 10^8 to 10^9 (CFU/g). All samples tested had a strong acidic taste with the pH of 4.0 to 4.3 and titrable acidity with 1.063 ` 1.321% lactic acid/ml. Acid production of isolated yoghurt starter increased during incubation. After 12 hr incubation, starter B showed the highest acid pro-duction ability but starter E was the lowest. All of starters were survived in artificial gastric juice and bile salts, especially starter B, E and I showed the strong resistance in artificial gastric juice and bile salts.

시판 요구르트 Starter의 생리적 특성

임용숙,김순희,이신호 대구효성가톨릭대학교 식품과학연구소 1994 식품과학지 Vol.6 No.-

시판 호상 요구르트의 품질 검사와 분리된 유산균의 starter 특성을 조사한 결과 시판 요구르트의 유산균 수는 9개 회사 시료중 1개 회사가 1.66×10^6CFU/g을 나타내었고 그외 8개 회사제품은 10^8~10^9CFU/㎖ 범위를 나타내었다. 제품의 pH 범위는 4.00~4.33으로 비교적 산미가 강하였으며, 적정산도는 1.063~1.321%였다. 순수 분리한 요구르트 starter의 산 생성 속도는 배양 시간이 경과함에 따라 적정산도가 모두 증가하는 것으로 나타났다. 특히, 배양 12시간째 starter B가 산 생성이 가장 좋았으며, starter E의 산 생성 속도가 가장 늦었다. 9개 회사 starter는 공히 위액과 담즙산에 대한 내성이 관찰되었으며 특히 D, E, G 균주의 경우 위액과 담즙산에 거의 생존하여 가장 내성이 강한 경향을 나타내었다. Viable cells, pH and titrable acidity of nine kinds of marketing gel-type yoghurts and acid production and survival in digestive fluids and bile salts of the isolated starters were investigated. Viable cells of one sample was 1.66×10^6(CFU/g) and other eight samples showed about 10^8 to 10^9(CFU/g). All samples tested had a strong acidic taste with the pH of 4.0 to 4.3 and titrable acidity with 1.063-1.321% lactic acid/㎖. Acid production of isolated yoghurt starter increased during incubation. After 12 hr incubation, starter B showed the highest acid production ability but starter E was the lowest. All of starters were survived in artificial gastric juice and bile salts, especially starter B, E and I showed the strong resistance in artificial gastric juice and bile salts.

한국인 파킨슨병에서 Monoamine Oxidase B 유전자와 Cytochrome P450IID6 유전자의 다형성

이용희,임정근,이상도,이영재 대한신경과학회 1999 대한신경과학회지 Vol.17 No.1

경사도 변화에 따른 트레드밀 역보행의 운동학적 분석

김석희,우병훈,임용규 漢陽大學校 體育科學硏究所 2003 體育科學 Vol.23 No.23

The purpose of this study is to understand special features of back ward walking through 3-dimensional kinematic analysis of backward walking inconsideration of treadmill gradient in 10 male university students in the in twenties and to provide the information required for deciding workload or for preventing injuries. In this stuidy, the walking speed of the treadmill was set at 3.Okm/h and the gradient of the treadmill was set at 0°, 5° and 10°. the APAS were used for the collection of image data and analysis. and one-way ANOVA was used to analyze the differences according to gradients. As there analysis and result show, The first, cadence at 0° was higher than at 5° and 10° The second, support time at 5° was longer than that of 0° and 10°The third, ankle joint displacement became generally low as gradient increased. The fourth, in the knee joint, displacement became higher as gradient increased in the early stage of backward walking. ; however, there was no difference at the later stage. Lastly, no angle displacement of hip joints showed in back ward walking. The study suggests that it will be more desirable to analyze kinetic variables on the backward walking. Therefore it needs the following study for the factor like that continuously.

Cu-SiO₂촉매상에서 메탄올의 메틸포메이트로의 탈수소 반응에 관한 연구

김경림,박해경,설용건,이태희,전민기 연세대학교 산업기술연구소 1990 논문집 Vol.22 No.2

Cu-SiO₂촉매상에서 구리의 함량을 달리하며 메탄올을 반응물질로 하여 탈수소 반응을 고정층 연속흐름 반응기에서 행하였다. 조작조건의 범위는 온도 175∼250℃, 압력 ?? 접촉시간 0.013∼0.043g.cat.hr/mL이었다. 구리의 함량에 따라 전화율과 선택도가 달라지고 구리의 함량이 1.5wt.%일 때 메틸포메이트로의 수율이 가장 좋았다. 최적 조작 조건은 온도와 접촉시간이 각각 225℃, 0.033g.cat.hr/mL 부근이었다. 이온 교환법으로 제조된 1.5wt.% Cu-SiO₂촉매상에서 메탄올의 탈수소 반응의 활성화 에너지는 3.73kcal/mol이었다. The dehydrogenation of methanol to methylformate was studied over Cu-SiO₂catalyst prepared by ion-exchange method with the change of copper content in a fixed bed, continuous flow reactor. The ranges of experimental conditions were : at the temperature between 175℃ and 250℃, pressure at ??, and contact time between 0.013 and 0.043g.cat.hr/mL. Conversion and selectivity were changed with Cu content and yield of methylformate showed the highest value with 1.5wt.% Cu. The optimum conditions were 225℃, 0.033g.cat.hr/mL for reaction temperature and contact time, respectively. The activation energy of dehydrogenation of methanol to methylformate was 3.73kcal/mol over 1.5wt.% Cu-SiO₂.

지구과학의 개념형성학습에 관하여 : 변화에 대한 개념을 중심으로 Especially Concerning to Concepts about Changing Phenomena

姜用熙,林成圭 경북대학교 과학교육연구소 1989 科學敎育硏究誌 Vol.13 No.-

By analyzing Earth Science textbooks currently used in high schools. we tried to establish a basic conceptual system concerning to changing phenomena based on the ESCP's structuralizing scheme. Investigating facts about time and change, we have found 21 basic concepts which should be understood through concept-forming teaching and learning. By classifying the basic concepts to fit the four principles suggested by NSTA as a scientific basic-system. we also tried to generalize the selected concepts. We made test problems in order to test high school students' understanding about earth science concepts especially concerning the changing phenomena in the field of astronomy. Using questionnaire method, we applied the test problems to 160 high school students in Taegu and Kyungpook areas. The results were as follows : the mean score of concept-learned group was 56.0 and that of unlearned group was 48.5. This means that the learned-group had been improved by concept-forming teaching.

이중구조 촉매전극을 이용한 알칼리 연료전지에 관한 연구

노용우,이태희,설용건,장의종,김경림 연세대학교 산업기술연구소 1990 논문집 Vol.22 No.1

An experimental low-temperature low-pressure hydrogen-oxygen alkali fuel cell was constructed and its performance was tested for various operating conditions. Hydrogen was used for fuel and oxygen was used for oxidant and KOH solution was used for electrolyte. Both electrodes for hydrogen and oxygen were prepared by the same method and the materials used were carbonyl nickel and ammonium bicarbonate. Experimental results show that maximum current density of 8.91 mA/㎠ was obtained at 40℃ wt% KOH solution and optimum power was 1.74 mW/㎠ at 6.43 mA/㎠ current density.

光照射에 의한 콩나물의 質的 變化

金明姬,元惠心,李正和,林容順,張美花 효성여자대학교 가정대학 학도호국단 1984 家政大論集 Vol.3 No.-

光의 照射에 의한 콩나물의 質的變化를 調査할 目的으로 光度 및 光質別 處理를 하여 成長狀態, Vitamin C, chlorphnyll, cellulose등의 含量과 質感에 對한 官能檢査를 行하였으며 chlorophyll과 vitamin C의 關係를 chloramphenicol 處理에 의하여 調査해 보았다. 그 結果 白色光의 경우 50 lux와 100 lux 處理로 vitamin C의 含量 增加와 cellulose등의 生成이 促進되었으며 質感이 暗所보다 良好하였다. 그러나 200 lux 하에서는 매우 不良하였다. blue光은 他光보다 cellulose와 vitamin C生成에 效果的 이었다. chlorophyll과 vitamin C의 含量과는 直接的인 關係가 없고 다만 光照射와의 關聯性이 있는 것으로 推定되었다. This study was carried out to evaluate the quality of green soybean sprouts grown under light. The soybean was germinated under light through blue, red and white polyethylene films at 25℃ for 10 hours a day, and light intensity was controlled 50 to 200 lux. The contents of Vitamin C. cellulose and chlorophyll were determined and checked up texture by sensory evaluation of the soybean sprouts soup. In addition, the relationship of chlorophyll and Vitamin C was measured by treatment of chloramphenicol which has inhibitory effect of chloroplast, chlorophyll accumulating site. Vitamin C and cellulose contents were all increased by irradiation of white light at 50 and 100 lux and also texture of the sprouts was better than it of the sprouts grown under darkness while, it was very tough under 200 lux. Blue light was more effective for biosynthesis of Vitamin C and cellulose than white and reg light. Biosynthesis of chlorophyll was not relative to Vitamin C content directly. but the relationship may be concerned with light irradiation.

産母의 血液所見이 新生兒 臍帶血中 血色素値에 미치는 影響

孫基勇,林英子,姜熙雄 金原出版 1969 小兒科 Vol.10 No.9