Study on thin copper-wire distribution for heat transfer enhancement from wall to interior of an isothermal chamber

Yang Lihong,Shen Hangming 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.4

This paper studied the distribution of thin copper wire in an isothermal chamber cross section through topological methods to enhanceheat transfer from the wall to the interior and to improve the isothermal characteristics of the isothermal chamber. First, the heat transfermodel was built and discretized by finite volume method. Then, the model was verified through the result of temperature field calculation,which was consistent with the ANSYS analysis under the condition that the copper wire was stuffed uniformly. Under the circumstancewhere the temperature of the container wall, the average stuffed density of thin copper wire, and the initial temperature in the center wereall the same as in the uniformly filled case, and taking the maximum of the center temperature as the optimization objective after heattransfer for 15 s, the linear variable density filling method and the adaptive growth method were used to optimize the copper wire distribution. Compared with the uniformly filled case, the results of the center temperature increased by 4.94% and 16.50%. Therefore, heatconduction from the wall to interior of the chamber can be enhanced by using topological methods to change the distribution of thin copperwires. Linear variable density filling method and adaptive growth method can be used to optimize the structure of thermal conductivity. Moreover, the result of adaptive growth method is better than the linear variable density filling method. During the isothermal chamberdischarge, the chamber wall is the most important heat resource. Thus, this method can be used to improve the characteristics of isothermalchambers.

miRNA-183 Suppresses Apoptosis and Promotes Proliferation in Esophageal Cancer by Targeting PDCD4

Yang, Miao,Liu, Ran,Li, Xiajun,Liao, Juan,Pu, Yuepu,Pan, Enchun,Yin, Lihong,Wang, Yi Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.12

In our previous study, miRNA-183, a miRNA in the miR-96-182-183 cluster, was significantly over-expressed in esophageal squamous cell carcinoma (ESCC). In the present study, we explored the oncogenic roles of miR-183 in ESCC by gain and loss of function analysis in an esophageal cancer cell line (EC9706). Genome-wide mRNA micro-array was applied to determine the genes that were regulated directly or indirectly by miR-183. 3'UTR luciferase reporter assay, RT-PCR, and Western blot were conducted to verify the target gene of miR-183. Cell culture results showed that miR-183 inhibited apoptosis (p < 0.05), enhanced cell proliferation (p < 0.05), and accelerated G1/S transition (p < 0.05). Moreover, the inhibitory effect of miR-183 on apoptosis was rescued when miR-183 was suppressed via miR-183 inhibitor (p < 0.05). Western blot analysis showed that the expression of programmed cell death 4 (PDCD4), which was predicted as the target gene of miR-183 by microarray profiling and bioinformatics predictions, decreased when miR-183 was over-expressed. The 3'UTR luciferase reporter assay confirmed that miR-183 directly regulated PDCD4 by binding to sequences in the 3'UTR of PDCD4. Pearson correlation analysis further confirmed the significant negative correlation between miR-183 and PDCD4 in both cell lines and in ESCC patients. Our data suggest that miR-183 might play an oncogenic role in ESCC by regulating PDCD4 expression.

Seasonal, Spatial and Inter-annual Variations in Composition of PM<SUB>2.5</SUB> in Four Typical Sites of Northern China

Lihong Ren,Gang Li,Xiaoyang Yang,Jingnan Hu 한국대기환경학회 2023 한국대기환경학회 학술대회논문집 Vol.2023 No.10

Clinical Characteristics of Patients with Persistent Apogeotropic and Persistent Geotropic Direction-Changing Positional Nystagmus

Si Lihong,Shen Bo,Li Yuanzhe,Ling Xia,Li Kangzhi,Yang Xu 대한신경과학회 2021 Journal of Clinical Neurology Vol.17 No.3

Background and Purpose This study aimed to determine the clinical features, diagnosis, and treatment of patients with persistent geotropic (pG) and persistent apogeotropic (pAG) direction-changing positional nystagmus (DCPN). Methods This retrospective study included 30 patients with pG-DCPN and 44 patients with pAG-DCPN. All patients underwent neurological and neurotological examinations, including an evaluation of gaze-evoked nystagmus, eye-movement tests, and assessments of limb ataxia and balance, as well as magnetic resonance imaging to exclude central causes. The characteristics of positional nystagmus were detected using the supine roll test (SRT) and bow-andlean test (BLT). The null point (NP) at which the nystagmus disappeared was determined. All patients were treated with the barbecue maneuver, and treatment efficacy was evaluated immediately, 1 week, and 1 month after treatment. Results The history of diseases associated with atherosclerosis, peripheral vestibular disorders, otological disease, and migraine differed significantly between patients with pG-DCPN and pAG-DCPN. The affected sides of persistent horizontal DCPN can be determined using the SRT and the BLT, while determining the second NP and vestibular function as well as performing an audiological evaluation can be used to assist in identifying the affected side. The efficacy rates immediately and 1 week after treatment with the barbecue maneuver were higher in patients with pAG-DCPN than in patients with pG-DCPN. Conclusions pAG-DCPN was more compatible with the characteristics of cupulolithiasis, and pG-DCPN was more likely to be associated with a light cupula rather than canalolithiasis. pAG-DCPN was more likely to be accompanied by a disease associated with atherosclerosis, while pG-DCPN was often accompanied by autoimmune-related diseases and a history of migraine. The associations between pAG-DCPN, pG-DCPN, and the above-mentioned diseases need to be clarified further. The canalith-repositioning maneuver was effective in patients with pAG-DCPN and ineffective in patients with pG-DCPN, but most cases of pGDCPN are self-limiting.

Process optimization for selective hydrogenation of α-pinene over Ni/AlPO4

Chen Yang,Lihong Jiang,Huaibo Wang,Yane Zheng,Yaming Wang 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.2

A new supported Ni/AlPO4 catalyst was synthesized and studied for the selective hydrogenation of α- pinene to prepare cis-pinane. The support was flaky morphology with orthorhombic phase and Ni was well dispersed. The surface area of the catalyst was 37.62m2·g−1 with a pore size of 2.83 nm. For the hydrogenation reaction, the performance of the catalyst was positively correlated with the surface area of support and loading content of Ni. Effects of hydrogenation condition were determined and the process was optimized by response surface methodology. The result suggested that the conversion was positively correlated to hydrogenation temperature, duration and catalyst dosage, while the selectivity showed a negative correlation to temperature and catalyst dosage. After optimization, 95.1% of selectivity was obtained under 94.8% of conversion at 405 K, 81 min and 2.28 wt% of catalyst.

miRNA-183 Suppresses Apoptosis and Promotes Proliferation in Esophageal Cancer by Targeting PDCD4

Miao Yang,Ran Liu,Xiajun Li,Juan Liao,Yuepu Pu,Enchun Pan,Lihong Yin,Yi Wang 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.12

In our previous study, miRNA-183, a miRNA in the miR-96-182-183 cluster, was significantly over-expressed in esophageal squamous cell carcinoma (ESCC). In the present study, we explored the oncogenic roles of miR-183 in ESCC by gain and loss of function analysis in an esophageal cancer cell line (EC9706). Genome-wide mRNA microarray was applied to determine the genes that were regulated directly or indirectly by miR-183. 3UTR luciferase reporter assay, RT-PCR, and Western blot were conducted to verify the target gene of miR-183. Cell culture results showed that miR-183 inhibited apoptosis (p < 0.05), enhanced cell proliferation (p < 0.05), and accelerated G1/S transition (p < 0.05). Moreo-ver, the inhibitory effect of miR-183 on apoptosis was rescued when miR-183 was suppressed via miR-183 inhibitor (p < 0.05). Western blot analysis showed that the expression of programmed cell death 4 (PDCD4), which was predicted as the target gene of miR-183 by microarray profiling and bioinformatics predictions, decreased when miR-183 was over-expressed. The 3'UTR luciferase reporter assay confirmed that miR-183 directly regulated PDCD4 by binding to sequences in the 3'UTR of PDCD4. Pearson correlation analysis fur-ther confirmed the significant negative correlation between miR-183 and PDCD4 in both cell lines and in ESCC patients. Our data suggest that miR-183 might play an oncogenic role in ESCC by regulating PDCD4 expression.

OPTIMAL PORTFOLIO STRATEGIES WITH A LIABILITY AND RANDOM RISK: THE CASE OF DIFFERENT LENDING AND BORROWING RATES

Zhaojun Yang,Lihong Huang 한국전산응용수학회 2004 Journal of applied mathematics & informatics Vol.15 No.-

This paper deals with two problems of optimal portfolio strate-gies in continuous time. The rst one studies the optimal behavior of a rmwho is forced to withdraw funds continuously at a xed rate per unit time.The second one considers a rm that is faced with an uncontrollable sto-chastic cash ow, or random risk process. We assume the rm’s incomecan be obtained only from the investment in two assets: a risky asset (e.g.,stock) and a riskless asset (e.g., bond). Therefore, the rm’s wealth followsa stochastic process. When the wealth is lower than certain legal level, therm goes bankrupt. Thus how to invest is the fundamental problem of therm in order to avoid bankruptcy. Under the case of dierent lending andborrowing rates, we obtain the optimal portfolio strategies for some reason-able objective functions that are the piecewise linear functions of the rm’scurrent wealth and present some interesting proofs for the conclusions. Theoptimal policies are easy to be operated for any relevant investor.

Diversity and Functions of Endophytic Fungi Associated with Roots and Leaves of Stipa purpurea in an Alpine Steppe at Qinghai-Tibet Plateau

( Xiaoyan Yang ),( Hui Jin ),( Lihong Xu ),( Haiyan Cui ),( Aiyi Xin ),( Haoyue Liu ),( Bo Qin ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.7

Stipa purpurea is a unique and dominant herbaceous plant species in the alpine steppe and meadows on the Qinghai-Tibet Plateau (QTP). In this work, we analyzed the composition and diversity of the culturable endophytic fungi in S. purpurea according to morphological and molecular identification. Then, we investigated the bioactivities of these fungi against plant pathogenic fungi and 1- aminocyclopropane-1-carboxylate deaminase (ACCD) deaminase activities. A total of 323 fungal isolates were first isolated from S. purpurea, and 33 fungal taxa were identified by internal transcribed spacer primers and grouped into Ascomycota. The diversity of endophytic fungi in S. purpurea was significantly higher in roots as compared to leaves. In addition, more than 40% of the endophytic fungi carried the gene encoding for the ACCD gene. The antibiosis assay demonstrated that 29, 35, 28, 37 and 34 isolates (43.9, 53.1, 42.4, 56.1, and 51.5%) were antagonistic to five plant pathogenic fungi, respectively. Our study provided the first assessment of the diversity of culturedepending endophytic fungi of S. purpurea, demonstrated the potential application of ACCD activity and antifungal activities with potential benefits to the host plant, and contributed to high biomass production and adaptation of S. purpurea to an adverse environment.

Synthesis of Carbon Dots from PEG6000 and Papain for Fluorescent and Doxycycline Sensing

Yuanjiao Feng,Lihong Tan,Qian Tang,Wenwu Zhong,Xiaoming Yang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.09

Herein, a simple-green road for preparing carbon dots (CDs) from the mixture of PEG6000 and papain with approving fluorescence has been successfully developed for the first time. Meanwhile, the current CDs were characterized by analytical means such as fluorescence, UV visible spectrophotometer, HR-TEM, XPS and FTIR and so on. Afterwards, this kind of CDs were employed for analyzing doxycycline (DC) relying on the mechanism that the functional group of DC had interactions with CDs, hence, leading to the fluorescence quenching. Also, this DC analytical method mentioned here permitted in a linear range of 5.0 x 10 -8 mol/L - 5.0 x 10 -4 mol/L as well as a detection limit of 18nM at a signal-to-noise ratio (S/N) of 3. More importantly, the CDs prepared here were still explored for milk real samples, indicating their potential for broadening avenues towards other various applications.

Characterization and functional analysis of the PtEXLA1 gene from poplar

Liu Jiafu,Wang Yan,Yang Lihong,Wang Xue,Zhang Junkang,Xu Jichen 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.1

Expansin plays a crucial role in plant growth and stress resistance as a cell wall relaxation protein. The expansin family consists of four subfamilies: EXPA, EXPB, EXLA, and EXLB. However, a few reports have been previously published investigating EXLA genes. The research here aimed to characterize the PtEXLA1 gene from a popular species (P. alba × P. glandulosa CV.84K) and evaluate its role through genetic transformation to understand its contribution to plant growth and stress resistance. The results showed that the PtEXLA1 gene was 780 bp in length, encoded 259 amino acids, and had typical characteristics of EXLA. The PtEXLA1 transgenic tobacco plants had a larger corolla in comparison to wild-type plants, and exhibited higher resistance to drought, high temperature, and salt stress based on the evaluation of chlorophyll content, relative conductivity, and malondialdehyde content. PtEXLA1 can be an efficient gene resource for stress resistance breeding of plants.