Effect of growth hormone releasing hormone on chondrocytes of osteoarthritis

( Zhuoran Li ),( Yao Li ) 대한내과학회 2022 The Korean Journal of Internal Medicine Vol.37 No.1

Background/Aims: To evaluate the effect and possible mechanism of growth hormone releasing hormone (GHRH) on chondrocytes of osteoarthritis (OA). Methods: Articular chondrocytes were cultured and the expression of GHRH receptor in chondrocytes was detected. Then recombinant adenovirus GHRH (Ad-GHRH) was transfected to one group of chondrocytes. The expression of collagen type II, matrix metalloproteinase 13 (MMP-13) and signal transducer and activator of transcription 3 (STAT3) in each experimental group was determined by Western blot. Results: The GHRH receptor was expressed in chondrocytes, and this provided a basis for further study of the role of GHRH in chondrocytes. Cell proliferation of the Ad-GHRH group was significantly higher than that of the OA group by CCK-8 assay. Compared with the OA-group, the protein expression of MMP13 was decreased in the Ad-GHRH group. Compared with the OA-group, the protein expression of collagen type II, phosphorylated STAT3 (P-STAT3) were increased in the Ad-GHRH group. Conclusions: Our results show that the GHRH receptor is expressed in chondrocytes. GHRH can promote the proliferation of chondrocytes and the synthesis of type II collagen, and increase the extracellular matrix, which is achieved by phosphorylated STAT3 protein.

Experimental study on the combined effects of obstacles and local water mist on gas explosion for maritime safety

Zhang Bin,Li Zhuoran,Xia Yuanchen,Shi Jihao,Zhang Jinnan,Wang Boqiao,Yu Jiahang,Qu Yanxu,Chen Li,Lin Yejin,Wu Wanqing 대한조선학회 2022 International Journal of Naval Architecture and Oc Vol.14 No.1

A variety of experimental configurations including different water mist obstacles, are used to investigate the combined effects of obstacles and water mist upon the gas explosion. The results demonstrate the 8 mm water mist can significantly inhibit the deflagration, while both 45 mm and 80 mm water mists exhibit the opposite effect under all the locally distributed positions of water spray nozzle without obstacles inside the vessel. When considering the obstacles, the 45 mm water mist starts to mitigate the deflagration and its mitigation effect is more significant than that induced by the 8 mm water mist. What's more, the 80 mm water mist can slow down the flame propagation speed while it would still lead to the gas explosion. Additionally, there remains unchanged about the effect of locally spraying 8 mm water mist upon deflagration at the initial stage of flame development as varying the position of obstacles, while the effects of mitigating deflagration by 45 mm and 80 mm water mist are decreased. The results will make contributions to design the arrangement of equipment and water mist configuration on the offshore platform or NG-fueled ship's engine room so as to mitigate the gas explosion accident.

Analysis of Global Sagittal Postural Patterns in Asymptomatic Chinese Adults

Panpan Hu,Miao Yu,Zhuoran Sun,Weishi Li,Liang Jiang,Feng Wei,Xiaoguang Liu,Zhongqiang Chen,Zhongjun Liu 대한척추외과학회 2016 Asian Spine Journal Vol.10 No.2

Study Design: A prospective imaging study. Purpose: To characterize the distribution of the global sagittal postural patterns in asymptomatic Chinese adults using Roussouly classification. Overview of Literature: The norms of sagittal parameters in asymptomatic Chinese population have been previously described, but no report described their global sagittal postural patterns as characterized by Roussouly classification. Methods: A cohort of 272 asymptomatic Chinese adults was recruited. Data was assimilated by reviewing the films for each subject. Sagittal parameters were measured and sagittal postural patterns were then determined according to Roussouly classification. The pattern distributions were compared across genders within the study cohort. We also compared the data across different ethnicities from our study and a previous study to further characterize Chinese sagittal postures. Results: The cohort included 161 males and 111 females, with mean age of 23.2±4.4 years. The average descriptive results were as below: pelvic incidence (PI) 46.4°±9.6°, thoracic kyphosis (TK) 24.2°±9.0°, lumbar lordosis (LL) 50.6°±10.6°, sacral slope (SS) 37.2°±7.6°, pelvic tilt (PT) 9.4°±6.8°, spinosacral angle (SSA) 131.1°±7.5° and sagittal vertical axis (SVA) 17.24±32.36 mm. Despite a significant difference between two genders in LL, PI, SSA, and SVA, no difference was found in the distribution of Roussouly types among them. 47.8% of our cohort belonged to Roussouly type 3, while type 1, 2 and 4 comprised 23.2%, 14.0% and 15.1% of the subjects, respectively. Roussouly classification was capable of categorizing sagittal parameters except for the PT. This study also found that 4.4% of the recruited subjects belonged to the C7-anterior subgroup. Conclusions: From a characterization of the sagittal postural patterns of asymptomatic Chinese adults using Roussouly classification, the distribution was similar between Chinese males and females; however, from a cross-study comparison, it was different between asymptomatic Chinese and Caucasian adults, with a higher proportion of Roussouly type 3 in Chinese adults.

The role of acupuncture and moxibustion in the treatment, prevention, and rehabilitation of patients with COVID-19: A scoping review

Mengjuan Ren,Yunlan Liu,Xiaojia Ni,Zhuoran Kuang,Xufei Luo,Yikai Zhang,Huishan Li,Yaolong Chen 한국한의학연구원 2022 Integrative Medicine Research Vol.11 No.4

Introduction This study aims to summarize the available evidence and guideline/consensus recommendations for acupuncture and moxibustion in the treatment, prevention and rehabilitation of patients with coronavirus disease 2019 (COVID-19). Methods A scoping review was performed. Eight electronic databases and other related websites were searched. All studies related to acupuncture and moxibustion for COVID-19 were considered. Descriptive analysis was applied to analyze the all included studies and guideline recommendations. Results We ultimately included 131 eligible studies. The main topics of the included studies were the treatment (82.4%) and prevention (38.9%) of COVID-19. The most included studies were literature reviews (65, 49.6%), protocols of systematic reviews (20, 15.3%), and guidelines and consensuses (18, 13.7%). The 18 (13.7%) COVID-19 guidelines and consensuses included 47 recommendations on acupuncture and moxibustion, which focused on the treatment (21/47, 44.7%), rehabilitation (17/47, 36.2%) and prevention (6, 12.8%) of COVID-19 patients. Zusanli (ST36), Feishu (BL13), Guanyuan (RN4) were recommended mostly for the treatment, rehabilitation and prevention respectively. Conclusion Acupuncture and moxibustion are effective in the treatment of COVID-19 patients to some extent. However, more high-quality of clinical trials still needed to determine the feasibility of acupuncture and moxibustion in COVID-19 patients to better guide clinical practice. Study registration Open Science Framework Registries (Registration DOI: 10.17605/OSF.IO/Z35WN; https://osf.io/z35wn). Introduction This study aims to summarize the available evidence and guideline/consensus recommendations for acupuncture and moxibustion in the treatment, prevention and rehabilitation of patients with coronavirus disease 2019 (COVID-19). Methods A scoping review was performed. Eight electronic databases and other related websites were searched. All studies related to acupuncture and moxibustion for COVID-19 were considered. Descriptive analysis was applied to analyze the all included studies and guideline recommendations. Results We ultimately included 131 eligible studies. The main topics of the included studies were the treatment (82.4%) and prevention (38.9%) of COVID-19. The most included studies were literature reviews (65, 49.6%), protocols of systematic reviews (20, 15.3%), and guidelines and consensuses (18, 13.7%). The 18 (13.7%) COVID-19 guidelines and consensuses included 47 recommendations on acupuncture and moxibustion, which focused on the treatment (21/47, 44.7%), rehabilitation (17/47, 36.2%) and prevention (6, 12.8%) of COVID-19 patients. Zusanli (ST36), Feishu (BL13), Guanyuan (RN4) were recommended mostly for the treatment, rehabilitation and prevention respectively. Conclusion Acupuncture and moxibustion are effective in the treatment of COVID-19 patients to some extent. However, more high-quality of clinical trials still needed to determine the feasibility of acupuncture and moxibustion in COVID-19 patients to better guide clinical practice. Study registration Open Science Framework Registries (Registration DOI: 10.17605/OSF.IO/Z35WN; https://osf.io/z35wn).

Efficacy of acupuncture for cardiopulmonary cerebral resuscitation: A systematic review and meta-analysis

Liu Yunlan,Ren Mengjuan,Kuang Zhuoran,Luo Xufei,Li Huishan,Zhang Yikai,Wen Wanxin,Cai Yefeng,Ni Xiaojia,Chen Yaolong 한국한의학연구원 2023 Integrative Medicine Research Vol.12 No.1

Background: Cerebral resuscitation is one of the main therapeutic aims in the treatment of cardiac arrest (CA) patients who experience a return of spontaneous circulation (ROSC). However, the therapeutic effects of current treatments are not ideal. The purpose of this study was to evaluate the efficacy of neurological function of acupuncture combined with conventional cardiopulmonary cerebral resuscitationthe (CPCR) for patients after ROSC. Methods: Seven electronic databases and other related websites were searched to identify studies on acupuncture combined with conventional CPCR for patients after ROSC. R software was used to conduct a meta-analysis, and the outcomes that could not be pooled were analyzed using a descriptive analysis. Results: Seven RCTs involving 411 participants who had experienced ROSC were eligible for inclusion. The main acupoints were Neiguan (PC6), Shuigou (DU26), Baihui (DU20), Yongquan (KI1), and Sanyinjiao (SP6). Compared to conventional CPCR, acupuncture combined with conventional CPCR led to signifi- cantly higher Glasgow Coma Scale (GCS) scores on day 3 (mean difference (MD) = 0.89, 95% CI: 0.43, 1.35, I 2 = 0%), day 5 (MD = 1.21, 95% CI: 0.27, 2.15; I 2 = 0%), and day 7 (MD = 1.92, 95% CI: 1.35, 2.50; I 2 = 0%). Conclusion: Acupuncture-assisted conventional CPCR may have a potential role in improving neurologi- cal function in CA patients after ROSC, but the certainty of evidence is very low and more high-quality studies are required.

An integrated method of flammable cloud size prediction for offshore platforms

Zhang Bin,Zhang Jinnan,Yu Jiahang,Wang Boqiao,Li Zhuoran,Xia Yuanchen,Chen Li 대한조선학회 2021 International Journal of Naval Architecture and Oc Vol.13 No.1

Response Surface Method (RSM) has been widely used for flammable cloud size prediction as it can reduce computational intensity for further Explosion Risk Analysis (ERA) especially during the early design phase of offshore platforms. However, RSM encounters the overfitting problem under very limited simulations. In order to overcome the disadvantage of RSM, Bayesian Regularization Artificial Neural (BRANN)-based model has been recently developed and its robustness and efficiency have been widely verified. However, for ERA during the early design phase, there seems to be room to further reduce the computational intensity while ensuring the model's acceptable accuracy. This study aims to develop an integrated method, namely the combination of Center Composite Design (CCD) method with Bayesian Regularization Artificial Neural Network (BRANN), for flammable cloud size prediction. A case study with constant and transient leakages is conducted to illustrate the feasibility and advantage of this hybrid method. Additionally, the performance of CCD-BRANN is compared with that of RSM. It is concluded that the newly developed hybrid method is more robust and computational efficient for ERAs during early design phase.

Involvement of DJ-1 in the pathogenesis of intervertebral disc degeneration via hexokinase 2-mediated mitophagy

Lin Jialiang,Wang Longjie,Wu Yuhao,Xiang Qian,Zhao Yongzhao,Zheng Xuanqi,Jiang Shuai,Sun Zhuoran,Fan Dongwei,Li Weishi 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-

Intervertebral disc degeneration (IDD) is an important pathological basis for degenerative spinal diseases and is involved in mitophagy dysfunction. However, the molecular mechanisms underlying mitophagy regulation in IDD remain unclear. This study aimed to clarify the role of DJ-1 in regulating mitophagy during IDD pathogenesis. Here, we showed that the mitochondrial localization of DJ-1 in nucleus pulposus cells (NPCs) first increased and then decreased in response to oxidative stress. Subsequently, loss- and gain-of-function experiments revealed that overexpression of DJ-1 in NPCs inhibited oxidative stress-induced mitochondrial dysfunction and mitochondria-dependent apoptosis, whereas knockdown of DJ-1 had the opposite effect. Mechanistically, mitochondrial translocation of DJ-1 promoted the recruitment of hexokinase 2 (HK2) to damaged mitochondria by activating Akt and subsequently Parkin-dependent mitophagy to inhibit oxidative stress-induced apoptosis in NPCs. However, silencing Parkin, reducing mitochondrial recruitment of HK2, or inhibiting Akt activation suppressed DJ-1-mediated mitophagy. Furthermore, overexpression of DJ-1 ameliorated IDD in rats through HK2-mediated mitophagy. Taken together, these findings indicate that DJ-1 promotes HK2-mediated mitophagy under oxidative stress conditions to inhibit mitochondria-dependent apoptosis in NPCs and could be a therapeutic target for IDD.