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      • KCI등재

        Adaptive State-of-Charge Estimation Method for an Aeronautical Lithium-ion Battery Pack Based on a Reduced Particle-unscented Kalman Filter

        Shun-Li Wang,Chun-Mei Yu,Carlos Fernandez,Ming-Jie Chen,Gui-Lin Li,Xiao-Han Liu 전력전자학회 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.4

        A reduced particle-unscented Kalman filter estimation method, along with a splice-equivalent circuit model, is proposed for the state-of-charge estimation of an aeronautical lithium-ion battery pack. The linearization treatment is not required in this method and only a few sigma data points are used, which reduce the computational requirement of state-of-charge estimation. This method also improves the estimation covariance properties by introducing the equilibrium parameter state of balance for the aeronautical lithium-ion battery pack. In addition, the estimation performance is validated by the experimental results. The proposed state-of-charge estimation method exhibits a root-mean-square error value of 1.42% and a mean error value of 4.96%. This method is insensitive to the parameter variation of the splice-equivalent circuit model, and thus, it plays an important role in the popularization and application of the aeronautical lithium-ion battery pack.

      • Cloning and Molecular Characterization of a Novel Rolling-Circle Replicating Plasmid, pK1S-1, from Bacillus thuringiensis subsp. kurstaki K1

        Ming Shun Li,Jong Yul Roh,Xueying Tao,Zi Niu Yu,Zi Duo Liu,Qin Liu,Hong Guang Xu,Hee Jin Shim,Yang-Su Kim,Yong Wang,Jae Young Choi,Yeon Ho Je 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

        Bacillus thuringiensis, an entomopathogenic bacterium belonging to the B. cereus group, harbors numerous extra-chromosomal DNA molecules whose sizes range from 2 to 250 kb. In this study, we used a plasmid capture system (PCS) to clone three small plasmids from B. thuringiensis subsp. kurstaki K1 using PCS which were not found in B. thuringiensis subsp. kurstaki HD-1, and determined the complete nucleotide sequence of plasmid pK1S-1 (5.5 kb). Of the six putative open reading frames (ORF2-ORF7) in pK1S-1, ORF2 (MobK1) showed approximately 90% aa identity with the Mob-proteins of pGI2 and pTX14-2, which are rolling circle replicating group VII (RCR group VII) plasmids from B. thuringiensis. In addition, a putative origin of transfer (oriT) showed 95.8% identity with those of pGI2 and pTX14-2. ORF3 (RepK1) showed relatively low aa identity (17.8-25.2%) with the Rep protein coded by RCR plasmids, however. The putative double-strand origin of replication (dso) and single-strand origin of replication (sso) of pK1S-1 exhibited approximately 70% and 64% identities with those of pGI2 and pTX14-2. ORF6 and 7 showed greater than 50% similarities with alkaline serine protease, which belongs to the subtilase family. The other 2 ORFs were identified as hypothetical proteins. To determine the replicon of pK1S-1, seven subclones were contructed in the B. t huringiensis ori-negative pHT1K vector and were electroporated into a plasmid cured B. thuringiensis strain. The 1.6 kb region that included the putative ORF3 (Rep1K), dso and ORF4, exhibited replication ability. These findings identified pK1S-1 as a new RCR group VII plasmid, and determined its replication region.

      • SCIESCOPUSKCI등재

        Adaptive State-of-Charge Estimation Method for an Aeronautical Lithium-ion Battery Pack Based on a Reduced Particle-unscented Kalman Filter

        Wang, Shun-Li,Yu, Chun-Mei,Fernandez, Carlos,Chen, Ming-Jie,Li, Gui-Lin,Liu, Xiao-Han The Korean Institute of Power Electronics 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.4

        A reduced particle-unscented Kalman filter estimation method, along with a splice-equivalent circuit model, is proposed for the state-of-charge estimation of an aeronautical lithium-ion battery pack. The linearization treatment is not required in this method and only a few sigma data points are used, which reduce the computational requirement of state-of-charge estimation. This method also improves the estimation covariance properties by introducing the equilibrium parameter state of balance for the aeronautical lithium-ion battery pack. In addition, the estimation performance is validated by the experimental results. The proposed state-of-charge estimation method exhibits a root-mean-square error value of 1.42% and a mean error value of 4.96%. This method is insensitive to the parameter variation of the splice-equivalent circuit model, and thus, it plays an important role in the popularization and application of the aeronautical lithium-ion battery pack.

      • KCI등재

        Association Study of Polymorphisms in Neuronal Nicotinic Acetylcholine Receptor Subunit Genes With Schizophrenia in the Han Chinese Population

        Yuan-yuan Li,Rui-jie Geng,Shun-ying Yu,Guan-jun Li,Zhou-ye Wang,Hua-fang Li 대한신경정신의학회 2021 PSYCHIATRY INVESTIGATION Vol.18 No.10

        Objective To investigate the relation between nicotinic acetylcholine receptor subunit (nAChR) genes and schizophrenia, and the relation between tag single nucleotide polymorphism (rs1317286, rs1044396, rs6494212, rs16969968, and rs684513) and schizophrenia in Han Chinese people. Methods The protein-protein interaction (PPI) network among nAChR protein and 350 proteins encoded by schizophrenia-related susceptibility genes was constructed through the String database to explore whether nAChR genes were associated with schizophrenia in these known databases. Then, five single nucleotide polymorphisms (SNPs) of CHRNA3 (rs1317286), CHRNA4 (rs1044396), CHRNA7 (rs6494212), and CHRNA5 (rs16969968, rs684513) were analyzed in a sample of 1,035 schizophrenic patients and 816 healthy controls. The interaction between the markers was analyzed using multifactor dimensionality reduction (MDR) software. Power analysis was performed using the Quanto program. Results There are no significant differences in genotype or allele distribution were identified between the patients and controls (p>0.05). The haplotypes constructed by four markers rs1317286, rs6494212, rs16969968, and rs684513 were not associated with schizophrenia either. However, a significant association between models made of rs1317286, rs1044396, rs6494212, and rs684513 and schizophrenia was revealed in interaction analysis (p<0.05). Conclusion The nAChR protein may have effects on the development of schizophrenia through the interaction with proteins encoded by schizophrenia-related susceptibility genes, but no relation was found between selected polymorphisms and schizophrenia in the collected Han Chinese people. However, interaction analysis suggested four-SNP model has an important effect on schizophrenia.

      • Identifying Differentially Expressed Genes and Small Molecule Drugs for Prostate Cancer by a Bioinformatics Strategy

        Li, Jian,Xu, Ya-Hong,Lu, Yi,Ma, Xiao-Ping,Chen, Ping,Luo, Shun-Wen,Jia, Zhi-Gang,Liu, Yang,Guo, Yu Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

        Purpose: Prostate cancer caused by the abnormal disorderly growth of prostatic acinar cells is the most prevalent cancer of men in western countries. We aimed to screen out differentially expressed genes (DEGs) and explore small molecule drugs for prostate cancer. Materials and Methods: The GSE3824 gene expression profile of prostate cancer was downloaded from Gene Expression Omnibus database which including 21 normal samples and 18 prostate cancer cells. The DEGs were identified by Limma package in R language and gene ontology and pathway enrichment analyses were performed. In addition, potential regulatory microRNAs and the target sites of the transcription factors were screened out based on the molecular signature database. In addition, the DEGs were mapped to the connectivity map database to identify potential small molecule drugs. Results: A total of 6,588 genes were filtered as DEGs between normal and prostate cancer samples. Examples such as ITGB6, ITGB3, ITGAV and ITGA2 may induce prostate cancer through actions on the focal adhesion pathway. Furthermore, the transcription factor, SP1, and its target genes ARHGAP26 and USF1 were identified. The most significant microRNA, MIR-506, was screened and found to regulate genes including ITGB1 and ITGB3. Additionally, small molecules MS-275, 8-azaguanine and pyrvinium were discovered to have the potential to repair the disordered metabolic pathways, abd furthermore to remedy prostate cancer. Conclusions: The results of our analysis bear on the mechanism of prostate cancer and allow screening for small molecular drugs for this cancer. The findings have the potential for future use in the clinic for treatment of prostate cancer.

      • KCI등재

        Granular Effects on Depositional Processes of Debris Avalanches

        Yu-xiang Hu,Hai-bo Li,Shun-chao Qi,Gang Fan,Jia-wen Zhou 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.4

        Debris avalanches generally have massive mobility and extensive deposited area, which have strong relation to the granular effect. The granular effect is an outcome from the interaction (based on frictional mechanism) among particles. Discrepancy of grain composition, for instance the difference of particle size and their proportion of particles, is a key factor to influence granular effect. A series of laboratory tests have been conducted to research the granular effects on depositional processes of debris avalanches containing fine- and coarse-grained particles. The velocity of debris front, the morphology characteristics and runout distance of the debris avalanche are considered in detail, and the mass parameter and the size parameter are introduced in analysis for the research of granular effect. The experimental results indicate that the granular effects can decrease the dissipation of momentum and increase the accumulation region, leading to further runout distance and more extensive deposited areas. Further, the velocity, the range of depositions of debris avalanches are larger than that in other conditions when the proportion of fine-grained particles is 30%, especially the materials consist of heterogeneous granular. It means there have a critical mass parameter in experiments which lead to the high efficiency of granular effects, and the granular effect is weakening in homogeneous granular composition.

      • SCISCIESCOPUS

        Transcription profile during maternal to zygotic transition in the mouse embryo

        Li, Xing-Yu,Cui, Xiang-Shun,Kim, Nam-Hyung CSIRO Publishing 2006 Reproduction, fertility, and development Vol.18 No.6

        <P> To gain insight into early embryo development, we used microarray technology to compare gene expression profiles in metaphase II oocytes and one- (1C), two- (2C) and four-cell (4C) embryos. Differences in spot intensities were normalised and grouped using Avadis Prophetic software platform (version 3.3; Strand Genomics, Bangalore, India) and categories were based on the PANTHER and Gene Ontology classification systems. We examined 6927 genes and identified those preferentially expressed in 1C or 2C embryos. We found 1261 genes that were more highly expressed (P < 0.05) in 1C compared with 2C embryos and 1480 genes showing enhanced expression in 2C embryos. Similarly, we investigated 6581 genes present in 2C and 4C embryos and identified 841 that are expressed to a greater extent at the 2C stage and 905 that are more highly expressed at the 4C stage. Using PANTHER classification, genes that were upregulated and downregulated in 2C embryos compared with 1C and 4C embryos were grouped according to their protein functions, which included developmental processes, electron transport, lipid, fatty acid and steroid metabolism, nucleoside, nucleotide and nucleic acid metabolism, protein metabolism and modification, signal transduction and transport, among others. Real time reverse transcription-polymerase chain reaction was performed to confirm differential expression of 14 selected genes. The identification of the genes being expressed will provide insight into the complex gene regulatory networks affecting zygotic genome activation and further development. </P>

      • SCOPUSKCI등재

        OSA-감자 덱스트린의 구조 및 유화 특성 연구

        한유진(Yu-Jin Han),이순희(Shun Ji Li),한정아(Jung-Ah Han) 한국식품과학회 2017 한국식품과학회지 Vol.49 No.1

        본 연구에서는 감자의 소비증진 및 식품산업에서의 활용가치를 높이기 위해 OSA기를 도입한 감자덱스트린을 제조하고, 덱스트린의 구조 및 물리적 특성과 실제 마요네즈 적용가능성을 살펴보았다. 호화개시온도는 초음파 처리한 시료와 산 가수분해 시료에서 유의적으로 감소하였으며, 호화 엔탈피 값은 70℃에서 초음파 처리한 시료에서만 유의적으로 크게 감소하였다. 페이스팅 점도특성은 초음파 처리 온도가 높아질수록 최고 점도는 감소하였으나, 산 처리 녹말에서는 오히려 최고점도가 증가하는 결과를 보였다. 감자녹말과 OSA-덱스트린 모두 B-타입의 결정도를 보였으나, 초음파 처리 온도가 높아질수록 상대적 결정도는 크게 감소하였고, 산 가수분해로 제조한 OSA-덱스트린의 경우 산 처리시간에 따른 유의적 변화는 없었으나 50℃에서 초음파 처리한 시료와 유사한 결정도를 보였다. OSA-녹말 또는 OSA-덱스트린의 유화안정성은 70℃에서 1시간 초음파 처리하여 제조한 시료가 가장 높았고, 산 가수분해 덱스트린>50oC에서 초음파 처리한 덱스트린= OSA녹말>25℃에서 초음파 처리한 덱스트린의 순이었다. OSA-덱스트린을 노른자위의 75%대체하여 제조한 마요네즈의 관능적 특성결과 색이나 맛에서는 모든 시료간 유의적 차이가 없었다. 전체적인 선호도에서는 노른자위첨가 마요네즈와 50 또는 70℃에서 1시간 초음파 처리한 OSA-덱스트린 첨가 시료 간에 유의적 차이가 없었으며, OSA-녹말첨가 마요네즈가 가장 낮은 점수를 받았다. 마요네즈의 유화안정성을 4주간 실험한 결과 1주 후에는 70℃에서 초음파 처리한 덱스트린 첨가 마요네즈가, 2주후부터는 1 시간 산 가수분해하여 제조한 덱스트린 첨가 마요네즈가 가장 높은 유화안정성을 보였으며, 2-4주까지는 노른자위첨가 마요네즈와 70℃에서 초음파 처리한 OSA-덱스트린 첨가 마요네즈가 유사한 안정성을 보였다. 반면, 50℃에서 초음파 처리한 OSA-덱스트린 첨가 마요네즈의 안정성은 저장기간 내내 가장 낮았다. 본 실험결과 OSA-감자녹말을 초음파 및 산 처리에 의해 제조한 덱스트린은 OSA녹말보다 유화력이 우수하였으며, 특히 70℃에서 1시간 초음파 처리한 덱스트린의 경우 노른자위를 대체하여 마요네즈나 저지방, 저콜레스테롤 식품제조에 다양하게 사용될 수 있을 것으로 생각된다. Octenyl succinylated (OSA) potato starch was dextrinized by two methods: ultrasound (at 25, 50, or 70oC for 1 h; OSA-25UT, OSA-50UT, and OSA-70UT, respectively) and acid hydrolysis (for 1 or 4 h; OSA-AD1H or OSA-AD4H, respectively), and the properties of the resulting starch were analyzed. The melting enthalpy of OSA-70UT decreased the most (from 14.0 to 10.0 mJ/mg), indicating chain degradation. For pasting properties, as ultrasound treatment temperature increased, peak viscosity decreased (2884, 2550, and 1888 cP, respectively), whereas acid hydrolysis increased peak viscosity and decreased pasting temperature. The relative crystallinity of OSA-dextrin produced by ultrasound or acid hydrolysis significantly decreased (from 33.61 to 14.90-26.03 and 19.28-20.05, respectively) as temperature or time increased, yet a B-type crystal pattern was maintained. Regarding emulsifying stability and sensory tests of mayonnaise prepared with OSA potato dextrin, mayonnaise with OSA-70UT was stable for short storage period (1 week), however mayonnaise with OSA-AD1H was the most suitable for long storage periods (from 2 to 4 weeks). In addition, the OSA- 70UT was the most acceptable for mayonnaise in the sensory test.

      • KCI등재

        ‘피동사-능동사’ 의미 비대칭 양상 연구

        리순녀(Li Shun-nv),박덕유(Park Deok-yu) 어문연구학회 2018 어문연구 Vol.96 No.-

        The purpose of this paper is to examine the meaning of passive verbs which has characteristic of Korean vernacular and the meaning difference of the corresponding active verb. In recent years, although studies focusing on passive education of Korean are still being carried out, but most of the studies so far have been conducted in the sentence structure and sentence pattern. However, there is few research on the passive verb meaning of the core part of the passive form. Korean learners should be made clear about the relationship between passive verbs and active verbs so that they can understand and use more passive expressions. In order to analyze the asymmetric aspects of the meaning of passive and active, this paper first compares the dictionary meanings of ‘hang’ and ‘be hung’, and based on this, their circular and extended meanings are examined with an image scheme. As a result, it is confirmed that it is asymmetric in the extended meaning although it shows the symmetrical pattern of ‘active – passive’ in the circular sense of ‘hang’ and ‘be hung’. It was found that the extended meaning of ‘be hung’ is more than the extended meaning of ‘hang’. ‘be hung’ and ‘hang’ have both the meaning of ‘real connection’ in the basic sense and ‘hangul’ in partial meaning symmetry with ‘be hung’ only in the meaning of virtual connection, request/trust relationship and affiliation relationship. In addition, there is a meaning of ‘be hung’ in terms of conditional relations, change relations, and causal relations, but it is confirmed that there is no such meaning in ‘hang’, and passive and active actors are not just 1: 1 relations of ‘passive - active’. Therefore, this study will be a basic theoretical study to review the passive education. Based on the cognitive semantics, this paper presents the semantic relation between passive and active. In particular, it aims to emphasi ze that it is more reasonable to understand and apply the meaning of vocabulary in terms of cognition. 본고의 목적은 한국어의 다의어 특징을 갖고 있는 피동사의 의미와 이에 대응되는 능동사의 의미 차이를 고찰하는 데 있다. 최근에도 한국어 피동교육을 중심으로 한 연구가 지속적으로 나오고 있지만 대부분 문장구조나 문형 차원에서 논의를 진행한 것으로 피동문의 핵심인 피동사의 의미에 대한 연구는 거의 찾아볼 수 없다. 한국어 학습자들에게 피동사와 능동사의 관계를 명확하게 밝히고 피동사가 다의어임을 인식하게 해야 더 많은 피동 표현을 이해하고 사용할 수 있다. 이에 본고는 다의어를 가장 잘 해석할 수 있는 인지의미론을 바탕으로 ‘걸리다’와 ‘걸다’를 중심으로 피동사와 능동사의 의미 차이를 제시하고 의미적 비대칭 양상을 기술하였다. 피동사와 능동사의 의미의 비대칭 양상을 분석하기 위해 우선 ‘걸리다’와 ‘걸다’의 사전적 의미를 비교하고 이를 바탕으로 그들의 원형적 의미와 확장 의미를 고찰하였다. 그 결과 ‘걸리다’와 ‘걸다’의 원형적 의미에서 ‘능동-피동’의 대칭 양상을 보이고 있지만 확장 의미에서는 비대칭임을 확인하였고, ‘걸리다’의 확장 의미가 ‘걸다’의 확장 의미보다 더 많음을 알 수 있었다. 그리고 ‘걸리다’와 ‘걸다’는 그 기본적 의미에서 모두 ‘실제적 연결’의 의미를 갖고 있고, 확장 의미에서 ‘걸리다’는 가상연결, 의뢰/의탁 관계, 소속 관계 의미에서만 ‘걸다’와 부분적 대칭을 보였다. 이외에 ‘걸리다’는 조건 관계, 변화 관계, 인과 관계 등에서 의미가 있지만 ‘걸다’는 이러한 의미가 전혀 없다는 것을 확인 하였다. 아울러 피동사와 능동사는 단지 ‘피동-능동’의 1:1 관계가 아님을 제시하였다. 본고는 인지적 의미론을 바탕으로 피동사와 능동사의 의미관계 양상을 제시하면서, 특히 인지적 측면에서 어휘의 의미를 이해하고 적용하는 것이 더 합리적임을 강조함으로써 한국어 피동사 교육에 대해 재검토하는 기초적 이론 연구가 되는 데 의의가 있다고 본다.

      • KCI등재

        Cloning and Molecular Characterization of a Novel Rolling-Circle Replicating Plasmid, pK1S-1, from Bacillus thuringiensis subsp. kurstaki K1

        Ming Shun Li,노종열,Xueying Tao,Zi Niu Yu,Zi Duo Liu,Qin Liu,Hong Guang Xu,심희진,김양수,왕용,최재영,제연호 한국미생물학회 2009 The journal of microbiology Vol.47 No.4

        Bacillus thuringiensis, an entomopathogenic bacterium belonging to the B. cereus group, harbors numerous extra-chromosomal DNA molecules whose sizes range from 2 to 250 kb. In this study, we used a plasmid capture system (PCS) to clone three small plasmids from B. thuringiensis subsp. kurstaki K1 which were not found in B. thuringiensis subsp. kurstaki HD-1, and determined the complete nucleotide sequence of plasmid pK1S-1 (5.5 kb). Of the six putative open reading frames (ORF2-ORF7) in pK1S-1, ORF2 (MobK1) showed approximately 90% aa identity with the Mob-proteins of pGI2 and pTX14-2, which are rolling circle replicating group VII (RCR group VII) plasmids from B. thuringiensis. In addition, a putative origin of transfer (oriT) showed 95.8% identity with those of pGI2 and pTX14-2. ORF3 (RepK1) showed relatively low aa identity (17.8~25.2%) with the Rep protein coded by RCR plasmids, however. The putative double- strand origin of replication (dso) and single-strand origin of replication (sso) of pK1S-1 exhibited approximately 70% and 64% identities with those of pGI2 and pTX14-2. ORF6 and 7 showed greater than 50% similarities with alkaline serine protease, which belongs to the subtilase family. The other 2 ORFs were identified as hypothetical proteins. To determine the replicon of pK1S-1, seven subclones were contructed in the B. thuringiensis ori-negative pHT1K vector and were electroporated into a plasmid cured B. thuringiensis strain. The 1.6 kb region that included the putative ORF3 (Rep1K), dso and ORF4, exhibited replication ability. These findings identified pK1S-1 as a new RCR group VII plasmid, and determined its replication region.

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