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Nadia Qureshi,Cynthia S. LeVesque,Dennis K. Bideshi,Brian A. Federici 한국응용곤충학회 2013 Journal of Asia-Pacific Entomology Vol.16 No.3
Four mRNA variants in pooled cDNA samples of the glassy-winged sharpshooter, Homalodisca coagulata,encoding a putative regulator of cation transporters were identified. RT-PCR showed that the hc-chaC variants were expressed during different stages of insect development and in different tissues and sexes. Structural analysis of the hc-chaC gene indicated that intron and exon sequences of the mRNA variants were identical, and similar to chaC-like genes of other insects. The Hc-ChaC protein (22.5 kDa) contained the conserved FGYGSL K+-binding motif near its amino-terminus, and the carboxy-terminal region contained two coiled-coil motifs,which had similarity to the PDZ domain present in well-characterized Na+/H+ exchanger regulatory factors. Our analyses suggest that Hc-ChaC is a regulator of K+ transporters such as K+/H+ antiporters.
Hydraulic Runner Design Method for Lifetime
Sabourin, Michel,Thibault, Denis,Bouffard, David-Alexandre,Levesque, Martin Korean Society for Fluid machinery 2010 International journal of fluid machinery and syste Vol.3 No.4
Quest for reliability of hydraulic runners is a concern for all mature electricity producers. The fatigue damage caused by dynamics loads is frequently the root cause of runner failure. This paper presents the damage tolerance approach based on fracture mechanics as the method chosen by Alstom and Hydro-Qu$\acute{e}$bec to predict effects of damage on runner lifetime and consequently to be use as a design method. This is sustained by a research on fracture mechanics properties of runner materials and by recommendations on the strategy to define a safety margin for design. The acquired knowledge permits to identify potential improvement of the runner lifetime without significant cost increase, like being more specific on some chemical composition or heat treatment.
Liu, Shaoshuai,Yan, Wenjuan,Ma, Chang,Liu, Yajing,Gong, Limin,Levesque, Crystal,Dong, Bing Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.4
Objective: The objective of this study was to investigate effects of supplementation of culture media from solid-state fermented Isaria cicadae (I. cicadae) on performance, serum biochemical parameters, serum immune indexes, antioxidant capacity and meat quality of broiler chickens. Methods: A total of 648 Arbor Acres male broiler chickens(1 d; average body weight, 42.93±0.47 g) were randomly assigned to 6 treatments, each with six replicates and 18 broiler chickens per replicate. Broiler chickens were fed phase I (d 1 to 21) and phase II (d 22 to 42) diets. The phase I diets were corn and soybean-meal based diets supplemented with 0%, 2%, 4%, 6%, 8%, or 10% culture media from solid-state fermented I. cicadae respectively. The phase II diets were corn and soybean-meal based diets supplemented with 0%, 1.33%, 2.67%, 4.00%, 5.32%, or 6.67% culture media from solid-state fermented I. cicadae respectively. Results: In phase I, the broiler chickens with the supplementation of culture media had increased body weight gain and feed intake (linear and quadratic, p<0.05) with increasing inclusion of culture media. The levels of serum total antioxidant capacity (T-AOC), glutathione (GSH) and superoxide dismutase (SOD) increased linearly (p<0.05). In phase II, levels of serum T-AOC and interleukin-1β increased linearly (p<0.05), and GSH increased (p<0.05). In the kidney, GSH and glutathione peroxidase (GSH-Px) concentrations increased (linear and quadratic, p<0.05) and SOD concentration increased linearly (p<0.05). Compared to the control, shear force and drip loss of breast muscle decreased (linear and quadratic, p<0.05). Drip loss of leg muscle decreased linearly and quadratically (p<0.05). Conclusion: Dietary supplementation of culture media from solid-state fermented I.cicadae which was enriched in both wheat and residual bioactive components of I. cicadae enhanced the growth performance of broiler chickens. It also improved body anti-oxidative status and contributed to improve broiler meat quality.
The Effects of Low Volume Versus High Volume Sled-Push Training on Muscular Adaptation
( Jeffrey R. Bernard ),( Yi-hung Liao ),( Christian O. Madrigal ),( Joshua D. Levesque ),( Matthew B. Fraze ),( Isaac Del Toro ),( Sukho Lee ) 한국운동생리학회(구 한국운동과학회) 2021 운동과학 Vol.30 No.2
PURPOSE: This study aimed to compare the effects of low-volume and high-volume sled-push resistance training on muscle strength, power, and body composition. METHODS: Twenty-four college students were recruited and matched based on baseline one-repetition maximum (1-RM) into one of the three groups: 1) low volume (LV) resistance training, 2) high volume (HV) resistance training, or 3) control (CON) (n=8 per group). The LV training consisted of five single repetitions of pushing a weighted sled for 9.1 m. The HV training consisted of three sets of five repetitions of pushing a weighted sled for 9.1 m. Training consisted of three weekly workouts performed on nonconsecutive days for 6 weeks. This study utilized a pre-test and post-test design consisting of 1-RM, Wingate power test, standing long jump, vertical jump, and body composition. RESULTS: After 6 weeks of training, there was a similar but significant increase in 1-RM in both training groups (pre-test: LV=226.8±14.8 kg vs. HV=217.7±19.5 kg; post-test: LV=298.5±15 kg vs. HV=286.9±16 kg, p< .001). However, no improvements were observed in the Wingate power test, standing long jump, vertical jump, or body composition in both training and CON groups (p >.05). CONCLUSIONS: The results suggested that low-volume resistance training was as effective as a high-volume protocol for improving muscle strength. However, the present study was unable to determine the effects on muscle power and body composition.
Schoch, Conrad L,Seifert, Keith A,Huhndorf, Sabine,Robert, Vincent,Spouge, John L,Levesque, C Andr?,Chen, Wen National Academy of Sciences 2012 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.109 No.16
<P>Six DNA regions were evaluated as potential DNA barcodes for Fungi, the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. The region of the mitochondrial cytochrome c oxidase subunit 1 used as the animal barcode was excluded as a potential marker, because it is difficult to amplify in fungi, often includes large introns, and can be insufficiently variable. Three subunits from the nuclear ribosomal RNA cistron were compared together with regions of three representative protein-coding genes (largest subunit of RNA polymerase II, second largest subunit of RNA polymerase II, and minichromosome maintenance protein). Although the protein-coding gene regions often had a higher percent of correct identification compared with ribosomal markers, low PCR amplification and sequencing success eliminated them as candidates for a universal fungal barcode. Among the regions of the ribosomal cistron, the internal transcribed spacer (ITS) region has the highest probability of successful identification for the broadest range of fungi, with the most clearly defined barcode gap between inter- and intraspecific variation. The nuclear ribosomal large subunit, a popular phylogenetic marker in certain groups, had superior species resolution in some taxonomic groups, such as the early diverging lineages and the ascomycete yeasts, but was otherwise slightly inferior to the ITS. The nuclear ribosomal small subunit has poor species-level resolution in fungi. ITS will be formally proposed for adoption as the primary fungal barcode marker to the Consortium for the Barcode of Life, with the possibility that supplementary barcodes may be developed for particular narrowly circumscribed taxonomic groups.</P>