Two new neolignans from Patrinia scabra with potent cytotoxic activity against HeLa and MNK-45 cells

Lei Di,Guo-Qing Yan,Ling-Yu Wang,Wei Ma,Kai-Jin Wang,Ning Li 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.10

Two new neolignans, patrineolignan A (1) andpatrineolignan B (2), together with seven known lignans,were isolated from the 90 % aqueous EtOH extract of theroots of Patrinia scabra. Their structures were elucidatedon the basis of spectroscopic data (HRESIMS, IR, 1D and2D NMR) and comparison with literature data. The twonew neolignans were evaluated in vitro for cytotoxicproperties against human cervical carcinoma HeLa cell lineand gastric carcinoma MNK-45 cell line using the microculturetetrazolium assay, and both 1 and 2 exhibitedstrongly cytotoxic activity against the two tumor cell lines.

Three New Iridoid Glucosides from the Roots of Patrinia scabra

Lei Di,You-Xing Zhao,Kai-Jin Wang,Ling-Bo Zu,Ning Li,Zhi Wang 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.9

To probe the chemical constituents of Patrinia scabra, we undertook the phytochemical investigation on its roots, which led to the isolation and elucidation of three new iridoid glucosides, scabroside A-C (1-3), along with three known iridoids, jatamanin J (4), isopatriscabroside I (5) and loganic acid (6) from the aqueous fraction of the ethanolic extract of the roots. The structures and relative configurations of the three new compounds were elucidated by spectroscopic methods including IR, UV, MS, 1D and 2D NMR experiments. Compound 3 was an unusual iridoid with an oxygen bridge connecting C-3 and C-8.

Chemical Constituents from Rhizomes of Curculigo capitulata

Lei Di,Kai-Jin Wang,Cui-Cui Zhu,Ning Li 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.10

EXAMINING THE USE OF WEATHER FORECASTS IN DIFFERENTIAL PRICING FOR SKI RESORTS

Cindy Yoonjoung Heo,Mara Leidi,Seob Gyu Song 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07

Climate change is expected to become a considerable threat to life on earth and it already affects certain tourism destinations like mountain regions and costal destinations. For instance, Switzerland is regarded as one of the most popular ski destination worldwide, but today’s ski resorts in Switzerland is facing significant difficulties as weather condition has been particularly harsh for the ski industry in the last years. While ski resorts start to use various rate fences to implement differential pricing, there is relatively little research how their customers perceive these new pricing approaches. Therefore, this study tried to identify how various rate fences adopted in ski resorts, including the use of weather forecasts in differential pricing, are perceived by customers and consequently which of them could be successfully implemented to provide the industry with long-term benefits. In particular, this study focused on the effect of framing and gender on price perception. The study found that female are generally more sensitive to differential pricing and have the tendency to perceive it as less fair.

Chemical composition of banana meal and rice bran from Australia or South-East Asia

Natalia S. Fanelli,Leidy J. Torres-Mendoza,Jerubella J. Abelilla,Hans H. Stein Asian Australasian Association of Animal Productio 2023 Animal Bioscience Vol.36 No.10

Objective: A study was conducted to determine the chemical composition of banana meal and rice bran from Australia or South-East Asia and test the hypothesis that there are no differences in rice bran produced in different countries, but there are differences between full-fat and defatted rice bran. Methods: Two sources of banana meal and 22 sources of rice bran (full-fat or defatted) from Australia or South-East Asia were used. All samples were analyzed for dry matter, gross energy, nitrogen, amino acids (AA), acid hydrolyzed ether extract (AEE), ash, minerals, total starch, insoluble dietary fiber, and soluble dietary fiber. Banana meal was also analyzed for sugars including glucose, fructose, maltose, sucrose, stachyose, and raffinose. Results: Chemical analysis demonstrated that banana meal from the Philippines is primarily composed of starch. Full-fat rice bran from Australia had greater (p<0.05) concentrations of AEE, lysine, and glycine than samples from the Philippines and Vietnam. Full-fat rice bran from Australia and Thailand had greater (p<0.05) concentrations of gross energy and most AA than rice bran from Vietnam. Full-fat rice bran from Australia had greater (p<0.05) concentrations of tryptophan and manganese than all other sources, but full-fat rice bran from the Philippines contained less (p<0.05) zinc than all other sources of rice bran. Gross energy, AEE, and copper were greater (p<0.05) in full-fat rice bran compared with defatted rice bran, but defatted rice bran contained more (p<0.05) crude protein, ash, insoluble dietary fiber, total dietary fiber, AA, and some minerals than full-fat rice bran. Conclusion: Banana meal is a high-energy source that can be used as an alternative ingredient in livestock diets. Full-fat rice bran from Australia and Thailand contained more concentrations of AEE and AA than samples from the Philippines or Vietnam. Full-fat rice bran had more gross energy and AEE than defatted rice bran, whereas defatted rice bran contained more crude protein, ash, and total dietary fiber.

Chemical composition of barley and co-products from barley, corn, and wheat produced in South-East Asia or Australia

Natalia S. Fanelli,Leidy J. Torres-Mendoza,Jerubella J. Abelilla,Hans H. Stein Asian Australasian Association of Animal Productio 2024 Animal Bioscience Vol.37 No.1

Objective: A study was conducted to determine the chemical composition of barley and co-products from barley, corn, and wheat produced in South-East Asia or Australia, and to test the hypothesis that production area or production methods can impact the chemical composition of wheat co-products. Methods: Samples included seven barley grains, two malt barley rootlets, one corn gluten feed, one corn gluten meal, one corn bran, eight wheat brans, one wheat mill mix, and four wheat pollards. All samples were analyzed for dry matter, gross energy, nitrogen, amino acids (AA), acid hydrolyzed ether extract, ash, minerals, starch, and insoluble dietary fiber and soluble dietary fiber. Malt barley rootlets and wheat co-products were also analyzed for sugars. Results: Chemical composition of barley, malt barley rootlets, and corn co-products were in general similar across countries. Wheat pollard had greater (p<0.05) concentrations of tryptophan, magnesium, and potassium compared with wheat bran, whereas wheat bran had greater (p<0.05) concentration of copper than wheat pollard. There were no differences in chemical composition between wheat bran produced in Australia and wheat bran produced in Thailand. Conclusion: Intact barley contains more starch, but fewer AA, than grain co-products. There were only few differences in the composition of wheat bran and wheat pollard, indicating that the two ingredients are similar, but with different names. However, corn gluten meal contains more protein and less fiber than corn bran.

Loss of halophytism by interference with SOS1 expression.

Oh, Dong-Ha,Leidi, Eduardo,Zhang, Quan,Hwang, Sung-Min,Li, Youzhi,Quintero, Francisco J,Jiang, Xingyu,D'Urzo, Matilde Paino,Lee, Sang Yeol,Zhao, Yanxiu,Bahk, Jeong Dong,Bressan, Ray A,Yun, Dae-Jin,Par American Society of Plant Physiologists 2009 Plant Physiology Vol.151 No.1

<P>The contribution of SOS1 (for Salt Overly Sensitive 1), encoding a sodium/proton antiporter, to plant salinity tolerance was analyzed in wild-type and RNA interference (RNAi) lines of the halophytic Arabidopsis (Arabidopsis thaliana)-relative Thellungiella salsuginea. Under all conditions, SOS1 mRNA abundance was higher in Thellungiella than in Arabidopsis. Ectopic expression of the Thellungiella homolog ThSOS1 suppressed the salt-sensitive phenotype of a Saccharomyces cerevisiae strain lacking sodium ion (Na(+)) efflux transporters and increased salt tolerance of wild-type Arabidopsis. thsos1-RNAi lines of Thellungiella were highly salt sensitive. A representative line, thsos1-4, showed faster Na(+) accumulation, more severe water loss in shoots under salt stress, and slower removal of Na(+) from the root after removal of stress compared with the wild type. thsos1-4 showed drastically higher sodium-specific fluorescence visualized by CoroNa-Green, a sodium-specific fluorophore, than the wild type, inhibition of endocytosis in root tip cells, and cell death in the adjacent elongation zone. After prolonged stress, Na(+) accumulated inside the pericycle in thsos1-4, while sodium was confined in vacuoles of epidermis and cortex cells in the wild type. RNAi-based interference of SOS1 caused cell death in the root elongation zone, accompanied by fragmentation of vacuoles, inhibition of endocytosis, and apoplastic sodium influx into the stele and hence the shoot. Reduction in SOS1 expression changed Thellungiella that normally can grow in seawater-strength sodium chloride solutions into a plant as sensitive to Na(+) as Arabidopsis.</P>

Chemical composition of copra, palm kernel, and cashew co-products from South-East Asia and almond hulls from Australia

Fanelli Natalia S,Torres-Mendoza Leidy J,Abelilla Jerubella J,Stein Hans H. 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.5

Objective: Oilseeds and nut co-products can be used as alternative feed ingredients in animal diets because they may have a lower cost than traditional ingredients. A study was, therefore, conducted to determine the chemical composition of copra, palm kernel, and nut coproducts from South-East Asia or Australia. The hypothesis that country of production influences nutritional composition was tested. Methods: Oilseed meals included 2 copra expellers, 3 copra meals, and 12 palm kernel expellers. One source of almond hulls and cashew nut meal were also used. Samples were obtained from suppliers located in South-East Asia or Australia. All samples were analyzed for dry matter, gross energy, nitrogen, amino acids (AA), acid-hydrolyzed ether extract (AEE), ash, minerals, insoluble dietary fiber, and soluble dietary fiber. Copra and nut coproducts were also analyzed for total starch and sugars. Results: Copra expellers had greater (p<0.05) concentrations of dry matter and AEE compared with copra meal. However, copra meal had greater (p<0.05) concentrations of total dietary fiber (soluble and insoluble) and copper than copra expellers. Palm kernel expellers from Indonesia had greater (p<0.05) concentration of histidine and tyrosine compared with palm kernel expellers from Vietnam. Almond hulls was high in dietary fiber, but also contained free glucose and fructose, whereas cashew nut meal was high in AEE, but low in all free sugars. Conclusion: Copra expellers have greater concentration of AEE, but less concentration of total dietary fiber when compared with copra meal, and except for a few AA, no differences in nutrient composition of palm kernel expellers produced in Indonesia or Vietnam were detected. According to the chemical composition of nut co-products, cashew nut meal may be more suitable for non-ruminant diets than almond hulls.

Pleiotropic effects of enhancing vacuolar K/H exchange in tomato

De Luca, Anna,Pardo, José,M.,Leidi, Eduardo O. Blackwell Publishing Ltd 2018 Physiologia Plantarum Vol.163 No.1

<P>Cation antiporters of the NHX family are widely regarded as determinants of salt tolerance due to their capacity to drive sodium (Na) and sequester it into vacuoles. Recent work shows, however, that NHX transporters are primarily involved in vacuolar potassium (K) storage. Over‐expression of the K/H antiporter AtNHX1 in tomato increases K accumulation into vacuoles and plant sensitivity to K deprivation. Here we show that the appearance of early leaf symptoms of K deficiency was associated with higher concentration of polyamines. Transgenic roots exhibited a greater sensitivity than shoots to K deprivation with changes in the composition of the free amino acids pool, total sugars and organic acids. Concentrations of amides (glutamine), amino acids (arginine) and sugars significantly increased in root, together with a reduction in malate and succinate concentrations. The concentration of pyruvate and the activity of pyruvate kinase were greater in the transgenic roots before K withdrawal although both parameters were depressed by K deprivation and approached wild‐type levels. In the longer term, the over‐expression of the NHX1 antiporter affected root growth and biomass partitioning (shoot/root ratio). Greater ethylene release produced longer stem internodes and leaf curling in the transgenic line. Our data show that enhanced sequestration of K by the NHX antiporter in the vacuoles altered cellular K homeostasis and had deeper physiological consequences than expected. Early metabolic changes lead later on to profound morphological and physiological adjustments resulting eventually in the loss of nutrient use efficiency.</P>

Is the variability in my crop due to genetic or environmental factors? Using heritability to solve this question

Peñuela Mauricio,Viáfara-Vega Ronald,Arias Leidy Laura,Cifuentes-Silva Héctor,López José,Cárdenas Heiber 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.2

Usually is common to see great diferences among plants in our crops, this can allow us to think about selection of some plants with the best traits in order to show that character in the next generation and thus improve our crops. Nevertheless, we forget that the observed phenotypic variance of our crop is due to several factors as genetic and environmental components. Knowing how these factors afect traits is an important question because it can tell us whether a selection program is viable or not, which can save efort, time, and money. Here, we present a case of study in a Tabasco chili pepper crop from southwestern Colombia where farmers generally practice selection ignoring the genetic and environmental components of the selected traits. To reveal these components, we proposed to use the estimation of heritability in a narrow sense, for which we developed measurements in two generations of plants and we applied a simple regression model in concordance with the expected inbreeding value for an autogamous species. Subsequently, we amplifed microsatellite molecular markers to support heritability values based on genetic diversity and confrmed whether the assumed inbreeding value for their estimation based on the life history of the species, was correct.