흰쥐의 좌골신경 절단손상후 기능회복에 대한 Acetyl-L carnitine의 효과

조주원,이정재,손유리,유영천,유석근 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

A possible therapeutic effect of acetyl-L carnitine (ALCAR) on peripheral nerve injuries and the expression of Jun, the protein products of immediate-early genes(IEGs), in the spinal cord were investigated after sciatic nerve injury in Sprague-Dawley rats. Experimental animals were divided into 3 groups: intact sciatic nerve as a control group, surgical repair alone, and surgical repair with ALCAR treatment. Toe-spreading response, pinprick response, and compound action potential were measured to evaluate the recovery of sciatic nerve injury. Recovery of behavioral function and electrophysiological function both improved in the surgical repair with ALCAR treatment group. Weak basal expression of Jun was shown in the ventral horn the of spinal cord in the control group. In the surgical repair alone group or the surgical repair with ALCAR treatment group, Jun expression persisted until 28 days after injury in the ventral horn of the spinal cord. Moreover, the surgical repair with ALCAR treatment group showed more increased expression of Jun than the surgical repair alone group. These results suggest that ALCAR facilitates the functional recovery after peripheral nerve injury.

최고 국내외 무용치료 현황 및 제도적 발전에 관한 연구

이유리 한국무용연구회 2007 한국무용연구 Vol.25 No.2

오늘날 현대를 살아가는 사람들은 알 수 없는 난치병에 걸려 현대의학의 보조 치료수단 으로 혹은 바쁜 지친 생활에서의 활력소를 찾기 위하여 대체의학에 의존하는 사례가 늘고 있다. 현대인들의 심신장애의 치유, 질병치료의 한 방법으로 예술세라피는 정신과 신체의 통 합적인 치료적 가치로 활발히 이루어지고 있다. 최근 예술치료의 한 분야인 무용치료에 관한 연구는 무용전공자들에 의해 점진적으로 관심이 배가되고 있을 뿐만 아니라 대체 의학적 측면에서도 연구가 집중되고 있다. 무용치료는 표현과 의사소통의 무용 움직임 고유의 기능을 가지고 정신과 신체의 조화 를 이루고 현대물질 사회의 병폐 속에서 심신의 안정과 정화에 도움을 준다. 또한 자기 자신을 자유롭게 창조 할 수 있고 그룹 내에 타인과의 관계 속에서 상호작용을 통해 융합 될 수 있는 기회를 제공한다. 이러한 가치들의 중요성을 인정하면서 무용치료 분야의 다양한 치료법들을 고찰하고 최근 무용치료 현황연구를 하고자 한다. 이를 위해 본 연구는 무용치료의 이론적 배경으로 무용치료의 원리적 이해와 서구 고 대 • 중세, 한국무속에서의 무용치료의 역사적 배경을 살펴보고, 최근 국내외 무용치료 현황과 제도적 발전 현황을 고찰하였다. 최근 국내외 무용치료 현황 연구를 위해 먼저 무용치료의 효용성 및 대상, 무용치료사 의 역할, 최근 한국과 외국의 무용치료 현황을 살펴봄으로서 최근 국내외 무용치료의 제도적 발전 현황을 연구하였다. 최근 무용치료의 제도적인 발전현황을 대학교 교육기관과 사회단체 연구기관으로 분리 하여 살펴보았다. 국내의 경우는 서울여자대학교 특수대학원, 세종대학교 사회교육원, 한국무용치료학회 에서 지도자양성이 활발히 이루어지고 있었다. 반면, 외국의 경우 무용치료는 미국을 중심으로 유럽 • 아시아로 발전 되어 왔다. 미국 은 대학교 • 대학원에서 무용치료 교육이 적극적이고 활발히 이루어지고 있음을 볼 수 있었다. 대학원에서 학위를 받은 후 연수과정을 통해 연구소. 사회복지 시설에서 치료사의 역할 이 활발히 이루어지고 있었다. 이와 같이, 무용치료는 현대를 살아가는 일반 성인, 청소년은 물론 장애를 가진 이들에 게 대체의학의 한 분야로 자리 매김하여 가고 있다. 그러므로 무용치료는 이론과 시술의 변형된 토대위에서 체계적으로 학습되어져야 한다. 나아가 적극적 전문 치료사 양성과 교육기관의 제도적 양성이 시급히 이루어져야 한다. Today more and more people are depending on alternative medicine to treat diseases incurable with modern medicine or vitalize themselves who are busy and tired in daily life. As an alternative medicine, art therapy is a method of treating modern people’s mental and physical disorders and diseases. It is valuable as a therapy that seeks to treat both mind body. Recently dance students are paying more attention to dance therapy as an art therapy. Dance therapy is also being intensively studied from the view of alternative medicine. Based on characteristic functions of dance movement, expression and communication, dance therapy helps accomplish harmony between mind and body and keep mental and physical stabilization and purification in modern material society. Dance therapy allows client to create themselves freely and get along with others through interactions within therapy groups. Thus the purpose of this study is to recognize these effects of dance therapy and review various technique and current conditions of the therapy. For the purpose, this researcher attempted to theoretically examine principles of dance therapy and developments of the therapy in the antique and medieval times of the West and in relation to Korean shamanism. Then the researcher investigated the current state and direction of dance therapy in this nation. In detail, the study considered the effectiveness and clients of dance therapy, roles of dance therapists and local and foreign cases of dance therapy. Recent institutional developments of dance therapy were investigated centering around colleges, universities, graduate schools, social groups and research institutes. In this nation, currently, dance therapists are being trained and cultivated by Special Graduate School of Seoul Women’s University, Social Education Institute of Sejong University and Korea Movement Therapy Association. While in regard to foreign nations, dance therapy actively began in U.S. and then extended to European and Asian countries. In U.S., dance therapy education is positively provided at colleges, universities and graduate schools. After receiving relevant degrees at graduate schools and then training, U.S. dance students role as dance therapists of research institutes and social welfare facilities. Today dance therapy is established as an alternatives medicine for adults, adolescents and even disabled persons. Thus dance therapy should be educated in a systematic way with theories and applications based. Further, agencies that actively train qualified, skilled dance therapists should be institutionalized.

Genetically engineered and self-assembled oncolytic protein nanoparticles for targeted cancer therapy

Lee, Joong-jae,Kang, Jung Ae,Ryu, Yiseul,Han, Sang-Soo,Nam, You Ree,Rho, Jong Kook,Choi, Dae Seong,Kang, Sun-Woong,Lee, Dong-Eun,Kim, Hak-Sung IPC Science and Technology Press 2017 Biomaterials Vol.120 No.-

<P><B>Abstract</B></P> <P>The integration of a targeted delivery with a tumour-selective agent has been considered an ideal platform for achieving high therapeutic efficacy and negligible side effects in cancer therapy. Here, we present engineered protein nanoparticles comprising a tumour-selective oncolytic protein and a targeting moiety as a new format for the targeted cancer therapy. Apoptin from chicken anaemia virus (CAV) was used as a tumour-selective apoptotic protein. An EGFR-specific repebody, which is composed of LRR (Leucine-rich repeat) modules, was employed to play a dual role as a tumour-targeting moiety and a fusion partner for producing apoptin nanoparticles in <I>E. coli</I>, respectively. The repebody was genetically fused to apoptin, and the resulting fusion protein was shown to self-assemble into supramolecular repebody-apoptin nanoparticles with high homogeneity and stability as a soluble form when expressed in <I>E. coli</I>. The repebody-apoptin nanoparticles showed a remarkable anti-tumour activity with negligible side effects in xenograft mice through a cooperative action of the two protein components with distinct functional roles. The repebody-apoptin nanoparticles can be developed as a systemic injectable and tumour-selective therapeutic protein for targeted cancer treatment.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Enhancement of 1,3-propanediol production by expression of pyruvate decarboxylase and aldehyde dehydrogenase from Zymomonas mobilis in the acetolactate-synthase-deficient mutant of Klebsiella pneumoniae

Lee, Sung-Mok,Hong, Won-Kyung,Heo, Sun-Yeon,Park, Jang Min,Jung, You Ree,Oh, Baek-Rock,Joe, Min-Ho,Seo, Jeong-Woo,Kim, Chul Ho Springer-Verlag 2014 Journal of industrial microbiology & biotechnology Vol.41 No.8

Selection of Trichodema reesei Mutant through Proton Beam Irradiation and Cellulase Production

You Ree JUNG,Seong Woo KANG,Hyun Yong SHIN,Jong Ho LEE,Seung Wook KIM 한국생물공학회 2009 한국생물공학회 학술대회 Vol.2009 No.10

두경부 편평상피세포암 세포주의 염색체 이상 분석:비교유전체보합법과 Array 비교유전체보합법

신유리(You Ree Shin),박수연(Soo-Yeun Park),이동욱(Dong Wook Lee),김한수(Han Su Kim),고영민(Young Min Go),박현주(Hyun Joo Park),정성민(Sung Min Choung) 대한두경부종양학회 2008 대한두경부 종양학회지 Vol.24 No.1

Head and neck squamous cell carcinoma(HNSCC) is notorious for its poor outcome and increasing incidence. But, the studies of cytogenetic analysis in HNSCC are relatively rare, because of difficulties in culturing solid tumor cells and complexity in chromosomal DNA abberations associated with the lesions. The purpose of this study is to evaluate the location of chromosomal aberrations in Korean HNSCC cell lines (SNU-1041, 1066, and 1076) with comparative genomic hybridization(CGH) and array based CGH(array-CGH). Chromosomal gains of 3q23-q27, 5p13-p15.3, 7p21-pter, 8q11.2-q12, 8q21.1-qter, 9q22-q34, 16q22-q24, and 20q11.2-qter, as well as chromosomal losses on 3p10-p14 were found in all 3 SNU cell lines. Losses on 3p15-p23, 4q22-q27, 4q31.3-qter, 6q14-q15, 7q31-q34, 8p12-pter, 18q21-q23, and 21q11.2-q12 were observed in 2 of 3 cell lines. In array-CGH, many genes were altered including gains of PIK3CA, MYC, EVI1, MAD1L1 genes and losses of SERPIN genes. These aberrations of gene and chromosome coincide with other results of study, generally. These data about the patterns of chromosomal aberrations could be a basic step for understanding more de-tailed genetic events in the carcinogenesis and also provide information for diagosis and treatment in HNSCC.

Facile radiolabeling of antibody-mimetic protein with In-111 via an inverse-electron-demand Diels-Alder reaction

Nam, You Ree,Shim, Ha Eun,Lee, Dong-Eun 대한방사성의약품학회 2019 Journal of radiopharmaceuticals and molecular prob Vol.5 No.2

In order to understand the in vivo biodistribution of repebody protein (RB), an efficient and simple radiolabeling method for the protein is needed. We demonstrate a detailed protocol for the radiosynthesis of an ¹¹¹In radiolabeled tetrazine prosthetic group and its application to the efficient radiolabeling of trans-cyclooctene-group conjugated repebody protein using inverse-electron-demand Diels-Alder reaction. First, 1,2,4,5-tetrazine (Tz) conjugated with a DOTA chelator, was used for preparing the radiolabeled DOTA complex with ¹¹¹In. Second, the trans-cyclooctene (TCO) functionalized repebody protein was synthesized which allows for the preparation of radiolabeled proteins by copper-free click chemistry. Following incubation with the ¹¹¹In-radiolabeled DOTA complex (¹¹¹In-Tz), the TCO-functionalized RB (TCO-RB) was radiolabeled successfully with ¹¹¹In, with a high radiochemical yield (69.5%) and radiochemical purity (>99%). The radiolabeling of repebody protein by copper-free click chemistry was accomplished within 20 min, with great efficiency in aqueous conditions. These results clearly indicate that the present radiolabeling method will be useful for the efficient and convenient radiolabeling of trans-cyclooctene-group containing biomolecules.

흉배동맥 관통분지 피판술을 이용한 연부조직 결손의 재건

유석근,이정재,손유리,유영천,조주원 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.5

In the case of a soft tissue defect which requires thin & pliable tissues, the choice of donor site is limited due to flap bulkiness and donor site morbidity. To overcome these problems, a variety of perforator-based flaps such as paraspinous and parasacral perforator flap, deep inferior epigastric perforator flap, gluteal perforator flap, and thoracodorsal artery perforator flap have recently been introduced. We experienced 8 cases of soft tissue defects from December 1996 to March 1999 using the thoracodorsal artery perforator flap for reconstruction. We could elevate the cutaneous flap with preservation of the latissimus dorsi muscle flap only when it was based on one cutaneous perforator. Defatting procedure was possible for further thinning of skin flap. In one case, axillary defect after release of postburn scar contracture was repaired with island perforator flap and the other seven cases were repaired with free flap. The results were satisfactory. We believe the thoracodorsal artery perforator flap is useful for reconstruction of soft tissue defects which are large or under conditions requiring thin flap.

기관절개술 반흔의 교정

유석근,손유리,유영천,김창욱,이정재 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.5

The tracheostomy is increasingly being performed. In most cases, the tracheostomy sites are left to heal by secondary intention, so it leaves a depressed and wide scar that is cosmetically disfigured. Another problem os that the scar is also attached directly to the trachea itself and will move vertically with the trachea during the act of swallowing. Even though the tracheostomy scar is cosmetically acceptable, the mobility and retraction of the scar is a continual nuisance to the patient. We performed a retrospective study on 9 patients who had undergone revision of the depressed thracheostomy scar by the Renner Method from June, 1997 to February, 1999. The method includes transverse fusiform incision of the original scar and excision of the depressed portion of the scar to the level of the trachea itself. To prevent attachment of the skin and trachea, a bilateral subcutaneous flap and muscle flap were simply advanced to the midline and overlapped. Then the rest of scar that was not depressed was deepithelized and flipped to augment the soft tissue volume in the central depressed area. Satisfactory results were achieved in all patients without hematoma, infection, hypertrophic scar, and keloid formation. We believe this simple Renner method is one of the best ways of performing posttracheostomy scar revision.

이차성 정맥성 허혈 후 피부판 생존에 미치는 PG E₁의 효과

정철훈,이종욱,범진식,오석준,신형식,손유리 大韓成形外科學會 1997 Archives of Plastic Surgery Vol.24 No.4

PG E₁ has been proven to be useful in the treatment of vascular disease of the lower leg because it induces vasodilatation of the peripheral vessel and platelet diaggregation and enhances red blood cell deformability. The effect of PG E₁ on tissue injury caused by secondary venous ischemia was evaluated in skin island flaps of 40 Sprague-Dawley rats. Primary ischemia was produced by arterio-venous occlusion with vascular clamps for 2 hours after the 3×5㎝ sized skin island flap based on the right superficial inferior epigastric vessels was elevated. Twenty four hours later, secondary ischemia was inflicted by venous occlusion for 6 hours. The rats were divided into 4 groups according to the timing of administration of PG E₁ or saline and each group had 10 rats. Normal saline(1.5 cc/kg) was injected at the beginning of secondary ischemia in group Ⅰ and PG E₁ 3 ㎍/kg(500 ng/kg/min) was injected intravenously at the end of primary ischemia in group Ⅱ. The same dose of PG E₁ was given at the beginning of secondary ischemia in group Ⅲ and at the end of secondary ischemia in Group Ⅳ. Normal saline or PG E₁ was injected through the left femoral vein. The full thickness skin biosies(0.5×0.5cm sized) were obtained from five rats each in group Ⅰ,Ⅱ and Ⅲ at the end of secondary ischemia and were examined under the light microscope with the H&E stain. The survival of skin flap that was assessed at 7 days after secondary ischemia showed all or none phenomenon. Group Ⅱ,Ⅲ,Ⅳ had a higher survival rats than the control group Ⅰ, but only group Ⅲ had statistical significance(P<0.005). The histologic findings of ischemic injury of the tissue such as vasodilatation, congestion, extravasation, thrombosis, endothelial cell disruption were decreased significantly in group Ⅲ compared to group Ⅰ. But neutrophil infiltration, one of the histologic findings of ischemic injury of the tissue, was increased in group Ⅲ. In conclusion, the survival rate of skin island flap based on the superficial inferior epigastric vessels increased significantly when PG E₁ was injected through contralateral femoral vein at the beginning of secondary venous ischemia. In case with venous thrombosis after free flap or digital replantation, it's fundamental treatment is revision of occluded vein as soon as possible. But if we detect venous occlusion early and administer PG E₁,it is able to decrease tissue damage.