http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Lee, Hyung-Min,Park, Rokjin J.,Henze, Daven K.,Lee, Seungun,Shim, Changsub,Shin, Hye-Jung,Moon, Kwang-Joo,Woo, Jung-Hun Elsevier Applied Science Publishers 2017 Environmental pollution Vol.221 No.-
<P><B>Abstract</B></P> <P>Enforcement of an air quality standard for <SUB> PM 2 . 5 </SUB> in the Seoul metropolitan area (SMA) was enacted in 2015. From May to June of 2016, an international airborne and surface measurement campaign took place to investigate air pollution mechanisms in the SMA. The total and speciated <SUB> PM 2 . 5 </SUB> concentrations since 2008 have been measured at an intensive monitoring site for the SMA operated by the National Institute of Environmental Research (NIER). To gain insight on the trends and sources of <SUB> PM 2 . 5 </SUB> in the SMA in May, we analyze <SUB> PM 2 . 5 </SUB> concentrations from 2009 to 2013 using the measurements and simulations from a 3-dimensional global chemical transport model, GEOS-Chem and its adjoint. The model is updated here with the latest regional emission inventory and diurnally varying <SUB> NH 3 </SUB> emissions. Monthly average <SUB> PM 2 . 5 </SUB> concentration measured by <I>β</I>-ray attenuation ranges from 28 (2010) to 45 (2013) <I>μ</I>g/m<SUP>3</SUP>, decreased from 2009 to 2010, and then continuously increased until 2013. The model shows good agreement with the measurements for the daily average <SUB> PM 2 . 5 </SUB> concentrations (R ≥ 0.5), and reproduces 10 out of 17 measured episodes exceeding the daily air quality standard (50 <I>μ</I>g/m<SUP>3</SUP>). Using the GEOS-Chem adjoint model, we find that anthropogenic emissions from the Shandong region have the largest modeled influence on <SUB> PM 2 . 5 </SUB> in Seoul in May. Average contributions to the high <SUB> PM 2 . 5 </SUB> episodes simulated by the model are 39% from the Shandong region, 16% from the Shanghai region, 14% from the Beijing region, and 15% from South Korea. Anthropogenic <SUB> SO 2 </SUB> emissions from South Korea are negligible with 90% of the total contribution originating from China. Findings from this study may guide interpretation of observations obtained in the KORUS-AQ measurement campaign.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Sources of PM2.5 of Seoul in May from 2009 to 2013 are investigated. </LI> <LI> Model emissions are updated with the new inventory for the best estimation. </LI> <LI> Contributions of Eastern China (69%) and South Korea (15%) to Seoul are identified. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Lee, Sang Wook,Ryu, Chae-Min,Shin, Jung-Hyun,Choi, Daeheon,Kim, Aram,Yu, Hwan Yeul,Han, Ju-Young,Lee, Hye-Yeon,Lim, Jisun,Kim, Yong Hwan,Heo, Jinbeom,Lee, Seungun,Ju, Hyein,Kim, Sujin,Hong, Ki-Sung,Ha Korean Continence Society 2018 International Neurourology Journal Vol.22 No.S1
<P><B>Purpose</B></P><P>To evaluate the therapeutic effect of human embryonic stem cell (hESC)-derived multipotent mesenchymal stem cells (M-MSCs) on ketamine-induced cystitis (KC) in rats.</P><P><B>Methods</B></P><P>To induce KC, 10-week-old female rats were injected with 25-mg/kg ketamine hydrochloride twice weekly for 12 weeks. In the sham group, phosphate buffered saline (PBS) was injected instead of ketamine. One week after the final injection of ketamine, the indicated doses (0.25, 0.5, and 1×10<SUP>6</SUP> cells) of M-MSCs (KC+M-MSC group) or PBS vehicle (KC group) were directly injected into the bladder wall. One week after M-MSC injection, the therapeutic outcomes were evaluated via cystometry, histological analyses, and measurement of gene expression. Next, we compared the efficacy of M-MSCs at a low dose (1×10<SUP>5</SUP> cells) to that of an identical dose of adult bone marrow (BM)-derived MSCs.</P><P><B>Results</B></P><P>Rats in the KC group exhibited increased voiding frequency and reduced bladder capacity compared to rats of the sham group. However, these parameters recovered after transplantation of M-MSCs at all doses tested. KC bladders exhibited markedly increased mast cell infiltration, apoptosis, and tissue fibrosis. Administration of M-MSCs significantly reversed these characteristic histological alterations. Gene expression analyses indicated that several genes associated with tissue fibrosis were markedly upregulated in KC bladders. However the expression of these genes was significantly suppressed by the administration of M-MSCs. Importantly, M-MSCs ameliorated bladder deterioration in KC rats after injection of a low dose (1×10<SUP>5</SUP>) of cells, at which point BM-derived MSCs did not substantially improve bladder function.</P><P><B>Conclusions</B></P><P>This study demonstrates for the first time the therapeutic efficacy of hESC-derived M-MSCs on KC in rats. M-MSCs restored bladder function more effectively than did BM-derived MSCs, protecting against abnormal changes including mast cell infiltration, apoptosis and fibrotic damage.</P>
Heo Jinbeom,Lee Jinyoung,Nam Yun Ji,Kim YongHwan,Yun HongDuck,Lee Seungun,Ju Hyein,Ryu Chae-Min,Jeong Seon Min,Lee Jinwon,Lim Jisun,Cho Yong Mee,Jeong Eui Man,Hong Bumsik,Son Jaekyoung,Shin Dong-Myung 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Aberrant activation of embryogenesis-related molecular programs in urothelial bladder cancer (BC) is associated with stemness features related to oncogenic dedifferentiation and tumor metastasis. Recently, we reported that overexpression of transcription factor CP2-like protein-1 (TFCP2L1) and its phosphorylation at Thr177 by cyclin-dependent kinase-1 (CDK1) play key roles in regulating bladder carcinogenesis. However, the clinical relevance and therapeutic potential of this novel CDK1-TFCP2L1 molecular network remain elusive. Here, we demonstrated that inhibitor of DNA binding-2 (ID2) functions as a crucial mediator by acting as a direct repressive target of TFCP2L1 to modulate the stemness features and survival of BC cells. Low ID2 and high CDK1 expression were significantly associated with unfavorable clinical characteristics. TFCP2L1 downregulated ID2 by directly binding to its promoter region. Consistent with these findings, ectopic expression of ID2 or treatment with apigenin, a chemical activator of ID2, triggered apoptosis and impaired the proliferation, suppressed the stemness features, and reduced the invasive capacity of BC cells. Combination treatment with the specific CDK1 inhibitor RO-3306 and apigenin significantly suppressed tumor growth in an orthotopic BC xenograft animal model. This study demonstrates the biological role and clinical utility of ID2 as a direct target of the CDK1-TFCP2L1 pathway for modulating the stemness features of BC cells.
이종건(Lee, Jong Keon),홍승근(Hong, Seungun) 한국인사관리학회 2014 조직과 인사관리연구 Vol.38 No.2
본 연구는 외식업에 있어서 리더-구성원 교환관계(LMX), 종업원 교육훈련, 업무몰입, 직무만족간의 관계를 분석하였다. 특히, 본 연구는 LMX와 업무몰입 간의 관계에 있어서의 종업원 교육훈련의 조절효과와 LMX와 직무만족 간의 관계에 있어서의 업무몰입의 매개효과를 분석하였다. 연구자료는 80개의 외식업 가맹점에 종사하는 218명의 종업원들을 대상으로 수집되었다. 본 연구결과는 다음과 같다. 첫째, LMX는 업무몰입 및 직무만족에 대하여 정(+)의 영향이 있으며, 종업원 교육훈련은 업무몰입에 대하여 정(+)의 영향이 있으나, 직무만족에 대한 유의적인 영향은 없는 것으로 나타났다. 둘째, 업무몰입은 직무만족에 대하여 정(+)의 영향이 있는 것으로 나타났다. 셋째, 업무몰입은 LMX와 직무만족 간의 관계를 부분적으로 매개하는 것으로 나타났다. 넷째, 종업원 교육훈련은 LMX와 업무몰입 간의 관계를 조절하는 것으로 나타났다. 마지막으로, 본 연구결과의 이론적 및 실무적 시사점에 대하여 논의하였다. We developed and tested a research model that examined the moderating role of employee training in the relationship between leader-member exchange (LMX) and work engagement and the mediating role of work engagement in the relationship between LMX and job satisfaction. Data were collected from 212 employees in 80 franchisees in Korea. The hypothesized relationships were tested using hierarchical multiple regression analysis. The results revealed that all hypotheses receive support from the data. The results indicated that LMX was positively related to work engagement and job satisfaction and that employee training was positively related to work engagement. Specifically, the results indicated that employee training moderated the impact of LMX on work engagement and that work engagement partially mediated the relationship between LMX and job satisfaction. Implications of the results are discussed and future research directions are offered.
Valproic acid enforces the priming effect of sphingosine-1 phosphate on human mesenchymal stem cells
Lim, Jisun,Lee, Seungun,Ju, Hyein,Kim, Yonghwan,Heo, Jinbeom,Lee, Hye-Yeon,Choi, Kyung-Chul,Son, Jaekyoung,Oh, Yeon-Mok,Kim, In-Gyu,Shin, Dong-Myung UNKNOWN 2017 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.40 No.3
<P>Engraftment and homing of mesenchymal stem cells (MSCs) are modulated by priming factors including the bioactive lipid sphingosine-1-phosphate (S1P), by stimulating CXCR4 receptor signaling cascades. However, limited <I>in vivo</I> efficacy and the remaining priming molecules prior to administration of MSCs can provoke concerns regarding the efficiency and safety of MSC priming. Here, we showed that valproic acid (VPA), a histone deacetylase inhibitor, enforced the priming effect of S1P at a low dosage for human umbilical cord-derived MSCs (UC-MSCs). A DNA-methylation inhibitor, 5-azacytidine (5-Aza), and VPA increased the expression of <I>CXCR4</I> in UC-MSCs. In particular, UC-MSCs primed with a suboptimal dose (50 nM) of S1P in combination with 0.5 mM VPA (VPA+S1P priming), but not 1 <I>µ</I>M 5-Aza, significantly improved the migration activity in response to stromal cell-derived factor 1 (SDF-1) concomitant with the activation of both MAPK<SUP>p42/44</SUP> and AKT signaling cascades. Both epigenetic regulatory compounds had little influence on cell surface marker phenotypes and the multi-potency of UC-MSCs. In contrast, VPA+S1P priming of UC-MSCs potentiated the proliferation, colony forming unit-fibroblast, and anti-inflammatory activities, which were severely inhibited in the case of 5-Aza treatment. Accordingly, the VPA+S1P-primed UC-MSCs exhibited upregulation of a subset of genes related to stem cell migration and anti-inflammation response. Thus, the present study demonstrated that VPA enables MSC priming with S1P at a low dosage by enhancing their migration and other therapeutic beneficial activities. This priming strategy for MSCs may provide a more efficient and safe application of MSCs for treating a variety of intractable disorders.</P>