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( Seon-jin Lee ),( Tae Woo Kim ),( Gyeong Lim Park ),( Yo Sep Hwang ),( Hee Jun Cho ),( Jong-tae Kim ),( Hee Gu Lee ) 생화학분자생물학회 2019 BMB Reports Vol.52 No.11
G protein-coupled estrogen receptor (GPER) is known to play an important role in hormone-associated cancers. G-1, a novel synthetic GPER agonist, has been reported to exhibit anti-carcinogenic properties. However, the chemotherapeutic mechanism of GPER is yet unclear. Here, we evaluated GPER expression in human gastric cancer tissues and cells. We found that G-1 treatment attenuates GPER expression in gastric cancer. GPER expression increased G-1-induced antitumor effects in mouse xenograft model. We analyzed the effects of knockdown/overexpression of GPER on G-1-induced cell death in cancer cells. Increased GPER expression in human gastric cancer cells increased G-1-induced cell death via increased levels of cleaved caspase-3, -9, and cleaved poly ADP-ribose polymerase. Interestingly, during G-1-induced cell death, GPER mRNA and protein expression was attenuated and associated with ER stress-induced expression of PERK, ATF-4, GRP-78, and CHOP. Furthermore, PERK-dependent induction of ER stress activation increased G-1-induced cell death, whereas PERK silencing decreased cell death and increased drug sensitivity. Taken together, the data suggest that the induction of ER stress via GPER expression may increase G-1-induced cell death in gastric cancer cells. These results may contribute to a new paradigm shift in gastric cancer therapy. [BMB Reports 2019; 52(11): 647-652]
Lee, Eun-Gyeong,Yoon, Sang-Hwal,Das, Amitabha,Lee, Sook-Hee,Li, Cui,Kim, Jae-Yean,Choi, Myung-Suk,Oh, Deok-Kun,Kim, Seon-Won Wiley Subscription Services, Inc., A Wiley Company 2009 Biotechnology and bioengineering Vol.102 No.1
<P>The amplification of gltA gene encoding citrate synthase of TCA cycle was required for the efficient conversion of acetyl-CoA, generated during vanillin production from ferulic acid, to CoA, which is essential for vanillin production. Vanillin of 1.98 g/L was produced from the E. coli DH5α (pTAHEF-gltA) with gltA amplification in 48 h of culture at 3.0 g/L of ferulic acid, which was about twofold higher than the vanillin production of 0.91 g/L obtained by the E. coli DH5α (pTAHEF) without gltA amplification. The icdA gene encoding isocitrate dehydrogenase of TCA cycle was deleted to make the vanillin producing E. coli utilize glyoxylate bypass which enables more efficient conversion of acetyl-CoA to CoA in comparison with TCA cycle. The production of vanillin by the icdA null mutant of E. coli BW25113 harboring pTAHEF was enhanced by 2.6 times. The gltA amplification of the glyoxylate bypass in the icdA null mutant remarkably increased the production rate of vanillin with a little increase in the amount of vanillin production. The real synergistic effect of gltA amplification and icdA deletion was observed with use of XAD-2 resin reducing the toxicity of vanillin produced during culture. Vanillin of 5.14 g/L was produced in 24 h of the culture with molar conversion yield of 86.6%, which is the highest so far in vanillin production from ferulic acid using recombinant E. coli. Biotechnol. Bioeng. 2009;102: 200–208. © 2008 Wiley Periodicals, Inc.</P>
Tissue-specific DNA damage response in Mouse Whole-body irradiation
Lee Seon-Gyeong,Kim Namwoo,박인배,Park Jun Hong,Myung Kyungjae 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.1
Background Genomic instability is a hallmark of various cancers, and DNA repair is an essential process for maintaining genomic integrity. Mammalian cells have developed various DNA repair mechanisms in response to DNA damage. Compared to the cellular response to DNA damage, the in vivo DNA damage response (DDR) of specific tissues has not been studied extensively. Objective In this study, mice were exposed to whole-body gamma (γ)-irradiation to evaluate the specific DDR of various tissues. We treated male C57BL6/J mice with γ-irradiation at different doses, and the DDR protein levels in different tissues were analyzed. Results The level of gamma-H2A histone family member X (γH2AX) increased in most organs after exposure to γ-irradiation. In particular, the liver, lung, and kidney tissues showed higher γH2AX induction upon DNA damage, compared to that in the brain, muscle, and testis tissues. RAD51 was highly expressed in the testis, irrespective of irradiation. The levels of proliferating cell nuclear antigen (PCNA) and ubiquitinated PCNA increased in lung tissues upon irradiation, suggesting that the post-replication repair may mainly operate in the lungs in response to γ-irradiation. Conclusion These results suggest that each tissue has a preferable repair mechanism in response to γ-irradiation. Therefore, the understanding and application of tissue-specific DNA damage responses could improve the clinical approach of radiotherapy for treating specific cancers.
( Gyeong Been Lee ),( Jienny Lee ),( Yong Woo Sohn ),( Na Yeon Gu ),( Hee Ryang Kim ),( Jeong Su Byeon ),( Hyung Seon Jeon ),( Jong Duck Jang ),( Young Jin Yang ),( In Soo Cho ),( Sang Ho Cha ) 한국예방수의학회(구 한국수의공중보건학회) 2015 예방수의학회지 Vol.39 No.3
Bone fractures are most often seen in racetrack horses because of the high level of intensity in racing. These issues are the main cause of decreased performance in racehorses. Mesenchymal stem cells (MSCs) have been explored to improve intra-articular therapy in racehorses. MSCs are essential for the repair and regeneration of damaged tissues. In this study, the effect of intra-articular injection of MSCs in racehorses was investigated. Before accessing the MSC therapy, synovial fluids were obtained from the fracture site of racehorses, and adipose tissue was collected for MSC isolation. Using the MSC specific marker, adipose tissue-derived MSCs were identified. The racehorses received intra-articular injection of autologous MSCs (or allogeneic) (3 × 107 cells/3 mL). After 1 or 2 weeks, synovial fluids were collected from racehorses. To test the effect of MSC injection using ELISA, we analyzed inflammatory factors from the untreated samples compared to MSC-treated samples of racehorses. The level of pro-inflammatory factors (interleukin-1β and prostaglandin E2) was significantly decreased in synovial fluids of MSC-injected racehorses, compared to before accessing the MSC therapy, whereas, the level of anti-inflammatory factor (interleukin-10) was higher than prior to accessing the MSC therapy. Further studies are needed to investigate the anti-inflammatory mechanism of MSC in racehorses.
이경선 ( Gyeong Seon Lee ) 미래유아교육학회 2011 미래유아교육학회지 Vol.18 No.3
The purposes of this study were to explore variables related to parental style of infant`s mother based on an ecological approach. Specifically, infants variables, mother`s variables, family variables, community variables were utilized for the analysis. Data analysis was conducted using responses of 1,033 families whose infant`s age was below 1-year-old from a nationally collected data set, 1st Panel Study of Korean Children of KICCE(Korea Institute of Child Care & Education). For analyzing the collected data, frequencies, percentages, stepwise multiple regressions were computed using SPSS package. The results of analysis were as follows. First, in terms of birth order and breast-feeding by mother, mothers who have lower birth order and breast-feeding had a positive parental style. Second, among the mother`s variables, parenting stress, self-esteem, economic activities, emotional children`s value, and academic background were found statistically significant in explaining parental style of infant`s mother. Third, the significant factors among family variables were father`s participating in raising their infants, marital satisfaction. Fourth, it was found that social support were signigicant among local community variables.