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- 저자 : Lee Hei Yung (검색결과 52 건)
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복압성요실금(Stress urinary incontinence)과 절박성요실금(Urge incontinence)환자에서 TVT 수술 결과의 비교
이경구,이준호,권용욱,정태융,심희영,이상익 관동대학교 의과대학 의과학연구소 2005 關東醫大學術誌 Vol.9 No.1
Tension-free vaginal tape(TVT) has been widely used in the management of stress urinary incontinence(SUI), and has been applied to urge incontinence (UI). We evaluated and compared the outcomes between SUI and UI for patients who had undergone TVT. Patients were categorized into SUI(33 patients) and UI(26 patients). Before treatment, we asked about LUTSs using female bladder questionnaire. And we asked about sexual function. After treatment, the same questionnaires were repeated. During the postperative period, the objective and subjective success rate were evaluated. For operation outcome results, in SUI group there were 29 cases of cure (87.9%)and 2 of improvement(6.1%), and in UI group there were 20 cases of cure(76.9%)and 3 of improvement(11.5%) (p>0.05). The operation satisfaction rate of patients was 90.9% in SUI group and 84.6% in UI group(p>0.05). Improvement of sexual satisfaction occurred in 6(20.0%) of SUI group, and 3 (18.8%) of UI group, respectively(p>0.05). The objective success rate, satisfaction rate and sexual satisfaction of SUI patients did not significantly differ from the women with UI. Also, these results shows that TVT procedure improves some LUTSs in both groups. We consider the TVT procedure to be an effective treatment not for stress urinary incontinence but also urge incontinenece.
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Lee, Jung-Eun,Yang, Suh-Yung,Lee, Hei-Yung The Korean Society for Integrative Biology 2000 Korean journal of biological sciences Vol.4 No.2
Genetic relationships among six species of the genus Rana from Korea were investigated by complete nucleotide sequence analyses of mitochondrial cytochrome b gene (1143 bp). Based on Kimura-2-parameter distance, the interspecific sequence differences of cytochrome b gene within the genus Rana were ranged from 7.83% to 25.00%. The genetic distances were 7.83% between R. nigromaculata and R. plancyi, 8.47% between two types of R. rugosa (type A and B), 10.42% between the brown frogs (R. amurensis and R. dybowskii), 16.11% between R. dybowskii types 1 and 2 and 12.36% between pond frogs (R. nigromaculata and R. plancyi) and R. catesbeiana. In the neighbor-joining and parsimony trees, R. catesbeiana was more closely related to pond frogs than brown frogs. R. dybowskii types 1 and 2 were considered to be at a distinct and specific level of differentiation (16.11%), while two types of R. rugosa were suspected to be at a subspecific level (8.47%).
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Intraspecific Variation of Cytochrome b Gene in Korean Frog , Rana amurensis
Lee, Hei Yung,Oh, Se Jo,Park, Ok Yi,Jin, Joung Hwa 한국유전학회 1996 Genes & Genomics Vol.18 No.4
A fragment(=350 base pair) of mtDNA at the cytochrome b gene site was amplified and sequenced to examine intraspecific variation of R. amurensis. The specific oligonucleotide primer set for polymerase chain reaction (PCR) was designed from a comparable published sequence of Xenopus leveis. Level of the partial cytochrome bgene sequence divergence was about 3% within species. The values were higher than those of another taxa, because certain regions of the sequence were identified as being particulaly differed. The results showed the those regions occur in 31-32 and 75, 78 (same numbering for the frog Xenopus laevis protein sequence). An interesting finding is that the regions showed variation within population. Therefore, these results suggest that the level of cytochrome b gene sequence divergence can be differed on the specific site. We have observed an example for the higher sequence divergence within species in partial cytochrome b gene.
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Lee, Hei Yung,Song, Jong Ho,Yang, Suh Yung 한국유전학회 1999 Genes & Genomics Vol.21 No.2
The partial sequences, 243 base pairs, of the mitochondrial cytochrome b gene were analyzed to infer genetic relationships among the Rana nigromaculata populations from Korea and Japan. Six different haplotypes were detected from 5 populations. The pairwise sequence differences were ranged from 2 - 4 bp among 5 haplotypes of Korean populations, and 15 - 18 bp between Korean and Japanese (Kyoto) haplotypes. Based on Tamura-Nei distance, average percent sequence divergence among the Korean haplotypes and between Korean and Japanese (Kyoto) haplotypes were 1.1% (0.8% - 1.7%) and 7.4% (6.6% - 8.0%) respectively. Contrary to the results of isozyme analysis which revealed the low level of genetic divergence (Nei's genetic distance D<0.1) between Korean and Japanese populations, the genetic variation of mtDNA haplotypes appeared to be highly differentiated between these two populations. The incompatibility of the genetic divergence between isozyme and mtDNA variations was discussed.
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노랑 초파리의 염색체에 존재하는 수컷 재조합 요소의 위치
Lee, Hei Yung,Lee, Kowan Ja 한국유전학회 1989 Genes & Genomics Vol.11 No.1
The frequencies of male recombination and site of the male recombination factor (MRF) were analysed with Song-nei strain of Drosophila melanogaster. The total frequency of male recombination was 0.076%. The frequency of male recombination in 2nd chromosome and 3rd chromosome were O.106% and 0.073% respectively. The sites of MRF were existed between b-c loci in 2nd chromosome and th-cu loci in 3rd chromosome. According to this result, MRF region was linked with heterochromatic region.
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Genetic Relationships of Rana amurensis Based on Mitochondrial Cytochrome b Gene Sequences
Lee, Jung-Eun,Yang, Dong-Eun,Kim, Yu-Ri,Lee, Hyuk,Lee, Hyun-Ick,Yang, Suh-Yung,Lee, Hei-Yung The Korean Society for Integrative Biology 1999 Korean journal of biological sciences Vol.3 No.3
Inter- and intraspecific genetic relationships between Rana amurensis from Korea and Russia and other brown frogs were investigated by nucleotide sequence of a 504 base pair (bp) fragment of the mitochondrial cytochrome b gene. Nucleotide sequence similarities among Korean populations of R. amurensis ranged from 99.6% to 97.6% and 98.8% within Russian populations. The nucleotide sequence similarity between Korean and Russian R. amurensis ranged from 86.9% to 85.5%. Based on Kimura-2-parameter distance, the sequence divergence between R. amurensis from Korea and Russia was 16.18% and 18.04% among other related brown frogs. interspecific sequence divergences among R. amurensis and other related brown frogs diverged by 20.3%. Using an estimate of 2-4% mitochondrial DNA sequence divergence per million years, Korean and Russian R. amurensis diverged about 8 to 4 million years ago (Mya) and other brown frogs diverged about 9 to 5 Mya from ancestral frogs and distributed from North Asia to Sakhalin in a short time. In the neighbor-joining and UPGMA tree R. amurensis was clustered into two groups with Korean and Russian populations and the other brown frogs were grouped separately with diverged trichotomous clusters (R. dybowskii and R. pirica, R. okinavana and R. tsushimensis, and R. japonica and R. longicrus).
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Lee, Hyuk,Yang, Suh-Yung,Lee, Hei-Yung The Korean Society for Integrative Biology 2000 Korean journal of biological sciences Vol.4 No.1
The genetic variations among six species of Rana from Korea (R. nigro-maculata, R. piancyi, R. dybowskii, R. sp, R. rugosa type A, B and R. amurensis) were investigated using 499 bases of mitochondrial DNA sequences for ND2 (NADH dehydrogenase subunit 2) gene and $tRNA^{Trp}$ gene. Partial sequences of ND2 gene (427 bp) and full sequences of $tRNA^{Trp}$ gene (73 bp) were identified. The level of sequence divergences ranged from 0.2 to 5.2% within species and 4.9-28.0% among 6 species of the genus Rana. The $tRNA^{Trp}$ gene of the genus Rana was composed of 77 nucleotides which showed a two dimensional "cloverleaf" structure. The secondary structure of $tRNA^{Trp}$ was not found compensatory changes which could potentially confound phylogenetic inference. In the neighborjoining tree, brown frogs were clustered first with the level of sequence divergence of 13.20% between R. amurensis and R. dybowskii, and 9% between R. dybowskii and R. sp. supported by 99% bootstrap iterations, respectively. R. nigromaculata and R. plancyi were clustered into another group with 5.1% divergence supported by 100% bootstrap iteration. R. rugosa A 8nd B types were grouped by 4.9% divergence and clustered into the last group with other two groups with 100% bootstrap iterations.
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한국산 맹꽁이의 미토콘드리아 Cytochrombe b 유전자의 유전적 변이
김유리,이정은,이혁,이혜영,양서영,양동은,이현익 한국유전학회 2000 Genes & Genomics Vol.22 No.2
The nucleotide sequences of 420 bp segment of cytochrome (cyt) b gene were analyzed using 14 specimens from Korea and 2 specimens from China. The level of mitochondrial DNA sequence differences ranged from 0% to 0.48% among seven popul-ations of Korean K. borealis, and 0.72% to 0.97% among Korean and Chinese K. borealis. In Korean K. borealis, the nucleotide substitution rates were ranged from 0% to 0.71% and all the nucleotide substitutions occurred by transition. Using Tamura-Nei distance, the level of sequence divergence ranged from 0.71% to 1.43% between Korean and Chinese K. borealis. According to the sequence divergence, the genetic variation among Korean and Chinise populations of K. borealis is very low. In the neighbor-joining tree, all the K, borealis samples were grouped in one.
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Kim, Yu-Ri,Yang, Dong-Eun,Lee, Hyuk,Lee, Jung-Eun,Lee, Hyun-Ick,Yang, Suh-Yung,Lee, Hei-Yung The Korean Society for Integrative Biology 1999 Korean journal of biological sciences Vol.3 No.2
The nucleotide sequences of a 504 bp segment of the mitochondrial cytochrome b gene were analyzed to survey the intraspecific variation of the brown frog, Rana dybowskii, collected from nine populations in South Korea. Comparisons of sequence divergence of the cytochrome b gene suggest that the populations examined are clearly classified into two types (type 1 and type 2), diverged from each other by a high value of 14.3-15.9% sequence divergence. The two types are distributed allopatrically in most populations, but only one population occurs sympatrically. In the Tonghae population, their spawning grounds differ in that type 1 spawns in the puddle and type 2 spawns in the mountain creek. Based on the genetic divergences of the cytochrome b gene sequences, the phylogenetic status of Korean R. dybowskii is elucidated by comparing it with related brown frogs distributed in an area adjacent to the Korean Peninsula. Interspecific sequence divergences among type 1, type 2 and other related brown frog species (Russian R. dybowskii, R. pirica, R. ornativentris, R. chensinensis: 2n=24 chromosomes) used in this study ranged from 11.7 to 16.3%. R. dybowskii in Tsushima is very similar to our type 1 (sequence divergence=0-1.6%) and R. chensinensis in western China is closest to our type 2 (sequence divergence=6.8-7.5%).
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