Peroxiredoxin II promotes hepatic tumorigenesis through cooperation with Ras/Forkhead box M1 signaling pathway

Park, Y-H,Kim, S-U,Kwon, T-H,Kim, J-M,Song, I-S,Shin, H-J,Lee, B-K,Bang, D-H,Lee, S-J,Lee, D-S,Chang, K-T,Kim, B-Y,Yu, D-Y Macmillan Publishers Limited 2016 Oncogene Vol.35 No.27

<P>The current study was carried out to define the involvement of Peroxiredoxin (Prx) II in progression of hepatocellular carcinoma (HCC) and the underlying molecular mechanism(s). Expression and function of Prx II in HCC was determined using H-ras(G12V)-transformed HCC cells (H-ras(G12V)-HCC cells) and the tumor livers from H-ras(G12V)-transgenic (Tg) mice and HCC patients. Prx II was upregulated in H-ras(G12V)-HCC cells and H-ras(G12V)-Tg mouse tumor livers, the expression pattern of which highly similar to that of forkhead Box M1 (FoxM1). Moreover, either knockdown of FoxM1 or site-directed mutagenesis of FoxM1-binding site of Prx II promoter significantly reduced Prx II levels in H-ras(G12V)-HCC cells, indicating FoxM1 as a direct transcription factor of Prx II in HCC. Interestingly, the null mutation of Prx II markedly decreased the number and size of tumors in H-ras(G12V)-Tg livers. Consistent with this, knockdown of Prx II in H-ras(G12V)-HCC cells reduced the expression of cyclin D1, cell proliferation, anchorage-independent growth and tumor formation in athymic nude mice, whereas overexpression of Prx II increased or aggravated the tumor phenotypes. Importantly, the expression of Prx II was correlated with that of FoxM1 in HCC patients. The activation of extracellular signal-related kinase (ERK) pathway and the expression of FoxM1 and cyclin D1 were highly dependent on Prx II in H-ras(G12V)-HCC cells and H-ras(G12V)-Tg livers. Prx II is FoxM1-dependently- expressed antioxidant in HCC and function as an enhancer of Ras(G12V) oncogenic potential in hepatic tumorigenesis through activation of ERK/FoxM1/cyclin D1 cascade.</P>

Calculations of AC magnetic losses from the experimental field profiles in various types of coated conductors under applied fields

Yoo, J,Lee, S,Jung, Y,Lee, J,Youm, D,Ha, H,Kim, H,Ko, R-K,Oh, S Institute of Physics 2008 Journal of physics. Conference series Vol.97 No.1

<P>We measured the field profiles, <I>H</I>(<I>x,H</I><SUB>a</SUB>) s, near the surface of coated conductors (CCs) by using the scanning Hall probe method. The samples were SmBCO-CC tape fabricated by co-evaporation method and YBCO-CC tape fabricated by PLD method. The applied fields, <I>H</I><SUB>a</SUB>s, were decreased from <I>H</I><SUB>peak</SUB>to -<I>H</I><SUB>peak</SUB> stepwise. From the values of <I>H</I>(<I>x,H</I><SUB>a</SUB>), we calculated the current profiles, <I>J</I>(<I>x,H</I><SUB>a</SUB>) s, by the inversion method. From the values of <I>J</I>(<I>x,H</I><SUB>a</SUB>) and the corresponding flux densities, we calculated the hysteretic energy losses per cycle, <I>Q</I><SUB>M</SUB>s, for various <I>H</I><SUB>peak</SUB>s. From the values of <I>Q</I><SUB>M</SUB>, we calculated the characteristic functions, <I>g</I>s, by using the relation, <I>g</I>= π<I>Q</I><SUB>M</SUB>/μ<SUB>0</SUB><I>I</I><SUP>2</SUP><SUB>c</SUB>. Here, <I>I</I><SUB>c</SUB> is the critical current. For the range of <I>H</I><SUB>peak</SUB>/<I>H</I><SUB>c</SUB>≤ 3, the <I>g</I>-values of SmBCO CC tape were larger than those of YBCO CC tape. However, for the range of <I>H</I><SUB>peak</SUB>/<I>H</I><SUB>c</SUB> ≥ 3, the <I>g</I>-values of SmBCO CC tape were smaller than those of YBCO CC tape. When <I>H</I><SUB>peak</SUB>/<I>H</I><SUB>c</SUB> = 3, both sample show almost same value of <I>g.</I>However we found qualitatively different <I>J–B</I> hysteretic curves for both samples. We also compared our <I>g</I>-values with other <I>g</I>-values, which were directly measured by energy loss experiments. Our <I>g</I>-values of YBCO CC tapes were basically similar to the Brandt's theoretical values of <I>g</I> in the most range of <I>I</I><SUB>peak</SUB> in our measurements.</P>

자외선의 조사간격이 브로일러 병아리의 볏 피부중 비타민 D₃함량에 미치는 영향

조인호,장윤환,이은택,여영수,배은경,김중달 한국축산학회 1994 한국축산학회지 Vol.36 No.1

This study was conducted to determine the content, of previtamin D₃(PreD₃), lumisterol₃(L₃), vitamin D₃(VD₃) and provitamin D₃(ProD₃) in comb skski of broiler chicks exposed to medium ware ultraviolet(UVB) lights in different interval. The broiler Hubbard line day old chicks(199 = 10 control + 3 irradiation interval × 9 elapsed time × 7 replica) were fed vitamin D deficient diet for 3 weeks in a windowless subdued light room and exposed to 297 ㎚ UVB light by 0.068 mJ/㎝-(10 min) three times in 0, 12 or 24 h interval. The comb skin were taken at 0, 6, 12, 18, 24, 48, 96, 144 or 240 h after last irradiation, and epidermis and dermis were separated. The lipid in sample was extracted by 9% ethyl acetate/hexane and purified by Sep-Pak silica catridge. The stright phase HPI-C was applied to analyze the concentration of Prop; and its photoproducts. When chicks were exposed once to UVB light for 30 min without interval, the mole % of ProD₃ in comb epidermis were 100% at control and 52.65% at 0 h after irradiation, thereafter it increased gradually to 88.17% at 240 h. PreD₃ and L₃ presented the maximum mole % at 0 h. VD₃ showed the peak value at 12 h. then decreased slowly. As UVB light was utilized to irradiate the chicks for 10 thin three times in 12 h interval, the ProD₃ mole portion in epidermis at 0 h was 76.4%, the lowest value among tested. PreD₃ and 1-3 preserved the highest level at 24 and 0 h, respectively, thereafter decreased gradully. VD₃ showed a peak at 6 h after exposure. When 24 h interval system was treated, the lowest value of ProD₃ 83.52% was appeared at 0 h. PreD₃ and L3 showed the highest level at 6 and 0 h, respectively. Mole ale of VD₃ had a peak value at 6 h and thin decreased. The mole % of ProD₃ and its photoproduets in comb dermis presented similar trends of time course variation as in those in epidermis. In respecting the method of UVB irradiation the PreD₃, L, and VDT were produced more quickly and largely in no intend system as compared to the time and amount produced in 12 or 24 h interval system.

급성 D. D. S. 중독증의 임상적 고찰

정시전 ( S. J. Chung ),최등영 ( D. Y. Choi ),이천각 ( C. K. Lee ),김형순 ( H. S. Kim ),태철현 ( T. H. Jeen ) 대한내과학회 1968 대한내과학회지 Vol.11 No.4

D.D.S. is well known as the drug of choice for Leprosy. Chronic D.D.S. intoxication has been reported in the many countries. It includes hematological changes, skin manifestations, abnormal liver function test and psychosis, There are few acute intoxicatio

Relationships of Plasma Insulin-like Growth Factor (IGF)-Ⅰ and IGF-ⅡConcentrations to Litter Size and Lactation Performance in Landrace and Yorkshire Pigs

Lee, C. Y.,Baik, K. H.,Lee, D. H.,Park, H. C. 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.1

본 연구는 혈장의 insulin-like growth factor(IGF)-Ⅰ과 IGF-Ⅱ 농도와 산자수 및 비유성적과의 관계를 구명하고자 착수되었다. 분만주가 비슷한 미경산 및 경산 임신돈 중에서 선발한 총 60두의 순종 Landrace와 Yorkshire 공시돈을 저산자수 (low; <μ - 0.5 SD)와 고산자수 (high; >μ + 0.5 SD)-lines로 구분하여 2 (품종) × 2(line) 요인분석 실험설계 하에 본 실험을 진행 하였다. 공시돈은 분만시 모돈당 포유자돈수를 9두로 고정시키고, 3주령 이유시 비유량의 척도로서 총 이유자돈 체중을 측정하였다. 혈액시료는 임신 90일과 분만 후 15일에 경정맥으로부터 채취하였다. 본 실험기간 중 산자수 및 본 실험기간까지의 평균 산자수는 high-line이 low-line보다 각각 3.7두와 2.4두 많았고 (P<0.01), 두 품종간 산자수 차이는 없었다. 임신 90일 혈장의 IGF-Ⅱ 농도는 low-line보다 high-line에서 높았다. 산자수는 임신 90일 IGF-Ⅰ 농도와 Landrace에서는 부의 상관관계를 나타냈고 (r=-0.46; P<0.05), Yorkshire에서는 부의 상관관계 경향을 나타내어 (r=-0.31; P=0.09) 전체 공시돈에서는 이들 두 변수간 유의적인 부의 상관관계를 보였다 (r=-0.35; P< 0.01). 이유시 모돈당 총 이유자돈 체중은 두 품종간 혹은 두 lines간 차이가 없었다. 이유자돈 체중과 IGF 농도와의 관계는 품종 × 생리적인 생태 조합들간 일정한 경향을 나타내지 않았다. 이상의 결과는 임신 90일 IGF 농도가 임박한 분만시 산자수의 지표가 될 수 있는 가능성을 시사한다. The present study was undertaken to find relationships of plasma insulin-like growth factor (IGF)-Ⅰand IGF-Ⅱ concentrations to litter size and lactation performance. Sixty pure-bred Landrace and Yorkshire pigs having similar farrowing weeks which had been selected from a large number of pregnant gilts and sows were divided into low- (<?? - 0.5SD) and high-litter size (>?? + 0.5 SD) lines under a 2 (breed) × 2 (line) factorial arrange of treatments. After adjusting the litter size to nine piglets per sow at farrowing, total litter weight was measured at three weeks postpartum at weaning as an index of milk yield. Blood samples were obtained from the jugular vein at day (d)-90 pregnancy (Px) and at d-15 postpartum. The litter size or the number of piglets born during the present experiment and the average litter size during the entire parities up to the present one were greater in the high-line than in the low-line by 3.7 and 2.4 piglets. respectively (P<0.01); effect of the breed on litter size was not significant. Plasma IGF-Ⅱ concentration at d-90 Px was greater in the high-line than in the low-line. Litter size and d-90 Px IGF-Ⅰconcentration were negatively correlated in Landrace (r=-0.46; P<0.05) and tended to be negatively correlated in Yokshire (r=-0.31; P=0.09), which resulted in a significant negative correlation between these two variables in total animals (r=-0.35; P<0.01). Litter weight at weaning was not different between the two breeds or lines. Relationships between the litter weight and IGF concentration were not consistent across the breed × physiological stage combinations. Results suggest that d-90 Px IGF concentrations may be indicative of the litter size at impending farrowing.

Cyclobuxine D의 토끼 적출 장관에 대한 작용

이종화,박영현,조병헌,김종배,김정목,김유재,김천숙,차영덕,김영석 최신의학사 1988 最新醫學 Vol.31 No.1

유착에 의한 AGS 및 Hep-G2 세포 표면 구조의 변화

박동규 ( D. K. Park ),전훈재 ( H. J. Chun ),박재홍 ( J. H. Park ),박철희 ( C. H. Park ),진윤태 ( Y. T. Jeen ),이홍식 ( H. S. Lee ),이상우 ( S. W. Lee ),엄순호 ( S. H. Um ),최재현 ( J. H. Choi ),김창덕 ( C. D. Kim ),류호상 ( H. S. Ryu 대한소화기학회 2002 대한소화기학회 춘계학술대회 Vol.2002 No.-

<목적> 최근 H. pylori 유착에 의한 세포 표면 구조의 변화에 관한 연구가 시도되어지고 있으나 actin 의 변화여부 및 그 특성에 관해서는 아직 명확히 정립되지 못한 실정이다. Rho GTPase는 세포 표면의 미세돌기인 microvilli, filopodia 및 membrane ruffle의 형성과 관련이 있으며, 최근 AGS 세포에서 H. pylori가 Rac activation에 의하여 membrane ruffle을 형성한다는 것과 Rac

Ahnak functions as a tumor suppressor via modulation of TGFβ/Smad signaling pathway

Lee, I H,Sohn, M,Lim, H J,Yoon, S,Oh, H,Shin, S,Shin, J H,Oh, S-H,Kim, J,Lee, D K,Noh, D Y,Bae, D S,Seong, J K,Bae, Y S Macmillan Publishers Limited 2014 Oncogene Vol.33 No.38

We provide detailed mechanisms of Ahnak-mediated potentiation of transforming growth factor β (TGFβ) signaling, which leads to a negative regulation of cell growth. We show that Smad3 interacts with Ahnak through MH2 domain and that Ahnak stimulates Smad3 localization into nucleus leading to potentiating TGFβ-induced transcriptional activity of R-Smad. Moreover, overexpression of Ahnak resulted in growth retardation and cell cycle arrest through downregulation of c-Myc and cyclin D1/D2. We describe results from analyses of Ahnak<SUP>−/−</SUP> mouse model expressing middle T antigen in a mammary gland-specific manner (MMTV<SUP>Tg/+</SUP>Ahnak<SUP>−/−</SUP>), which showed significantly progressed hyperplasia of mammary glands compared with MMTV<SUP>Tg/+</SUP>Ahnak<SUP>+/+</SUP>. Finally, we screened multiple human breast cancer tissues and showed that the expression of Ahnak in cancer tissues is lower than that in control tissues by 50%. Taken together, these data indicate that Ahnak mediates a negative regulation of cell growth and acts as novel tumor suppressor through potentiation of TGFβ signaling.

Generation of reassortant influenza viruses within the non-industrial poultry system

Lee, H.J.,Lee, D.H.,Lee, Y.N.,Kwon, J.S.,Lee, Y.J.,Lee, J.B.,Park, S.Y.,Choi, I.S.,Song, C.S. Elsevier Science 2012 INFECTION GENETICS AND EVOLUTION Vol.12 No.5

We compared the genetic and biologic characteristics of 35 influenza viruses of different epidemiological backgrounds in Korea, including H3N2 canine influenza virus (CIV). Phylogenetic analysis revealed that chicken adapted H9N2 viruses (A/chicken/Korea/96006/96 [CK/Kor/96006-like]) have acquired aquatic avian gene segments through reassortment, and these reassorted H9N2 viruses were more frequently detected from minor poultry species than from industrial poultry. Conversely, gene segments from CK/Kor/96006-like viruses were also detected in most of the viruses from domestic ducks. Interestingly, domestic ducks, rather than wild aquatic birds, harbored close relatives of all eight gene segments of H3N2 CIV, which preferred binding to avian receptors. Therefore, bidirectional virus transmission events are assumed to have occurred between land-based poultry and aquatic poultry, in particular within the non-industrial poultry system. These events have contributed to the generation of a novel reassortant, H3N2 CIV. To prevent generating other reassortants capable of interspecies transmission, gene movements in the non-industrial poultry systems should be clarified and managed.

Effect of Anodic Gas Compositions on the Overpotential in a Molten Carbonate Fuel Cell

Lee C.G.,Kim D.H.,Hong S.W.,Park S.H.,Lim H.C. The Korean Electrochemical Society 2006 한국전기화학회지 Vol.9 No.2

Anodic overpotential has been investigated with gas composition changes in a $100cm^2$ class molten carbonate fuel cell. The overpotential was measured with steady state polarization, reactant gas addition (RA), inert gas step addition (ISA), and electrochemical impedance spectroscopy (EIS) methods at different anodic inlet gas compositions, i.e., $H_2:CO_2:H_2O=0.69:0.17:0.14\;atm\;and\;H_2:CO_2:H_2O=0.33:0.33:0.33\;atm$, at a fixed $H_2$ flow rate. The results demonstrate that the anodic overpotential decreases with increasing $CO_2\;and\;H_2O$ flow rates, indicating the anode reaction is a gas-phase mass-transfer control process of the reactant species, $H_2,\;CO_2,\;and\;H_2O$. It was also found that the mass-transfer resistance due to the $H_2$ species slightly increases at higher $CO_2\;and\;H_2O$ flow rates. EIS showed reduction of the lower frequency semi-circle with increasing $H_2O\;and\;CO_2$ flow rate without affecting the high frequency semi-circle.