Adenosine triphosphate-based chemotherapy response assay-guided chemotherapy in unresectable colorectal liver metastasis

Hur, H,Kim, N K,Kim, H G,Min, B S,Lee, K Y,Shin, S J,Cheon, J H,Choi, S H Nature Publishing Group 2012 The British journal of cancer Vol.106 No.1

<P><B>Background:</B></P><P>This study aims to evaluate the effectiveness of adenosine triphosphate-based chemotherapy response assay (ATP-CRA)-guided neoadjuvant chemotherapy for increasing resectability in patients with unresectable colorectal liver metastasis.</P><P><B>Patients and methods:</B></P><P>Patients were randomised into two groups: Group A was treated by conventional chemotherapy regimen and Group B was treated by chemotherapy regimen according to the ATP-CRA. Three chemotherapeutic agents (5-fluorouracil, oxaliplatin and irinotecan) were tested by ATP-CRA and more sensitive agents were selected. Either FOLFOX or FOLFIRI was administered. Between Group A and B, treatment response and resectability were compared.</P><P><B>Results:</B></P><P>Between November 2008 and October 2010, a total 63 patients were randomised to Group A (<I>N</I>=32) or Group B (<I>N</I>=31). FOLFOX was more preferred in Group A than in Group B (26 out of 32 (81.3%) <I>vs</I> 20 out of 31 (64.5%)). Group B showed better treatment response than Group A (48.4% <I>vs</I> 21.9%, <I>P</I>=0.027). The resectability of hepatic lesion was higher in Group B (35.5% <I>vs</I> 12.5%, <I>P</I>=0.032). Mean duration from chemotherapy onset to the time of liver resection was 11 cycles (range 4–12) in Group A and 8 cycles (range 8–16) in Group B.</P><P><B>Conclusion:</B></P><P>This study showed that tailored-chemotherapy based on ATP-CRA could improve the treatment response and resectability in initially unresectable colorectal liver metastasis.</P>

1998년 분리된 이질균의 역학적 특성

이복권,강연호,김성한,유재연,전정훈 국립보건연구원 1998 국립보건원보 Vol.35 No.-

한국의 HIV-1 감염의 분자역학

이주실,김은영,남정구,기미경,최병선,김성순,강춘,구본기,신영오 국립보건연구원 1998 국립보건원보 Vol.35 No.-

국내 감염성질환 병원체의 성상분석을 통한 표준실험실 체계 구축(Ⅱ) : 장내바이러스와 B형간염바이러스 국내 분리주의 성상분석(1)

윤재득,지영미,김기순,천두성,안정배,정윤석,신수연,이선화,이지원,조해월 국립보건연구원 2000 국립보건원보 Vol.37 No.-

Aggravation of post-ischemic liver injury by overexpression of A20, an NF-κB suppressor

Yu, J.,Lee, H.S.,Lee, S.M.,Yu, H.C.,Moon, W.S.,Chung, M.J.,Park, J.W.,Park, B.H. Elsevier Science Publishers 2011 Journal of hepatology Vol.55 No.2

Backgroud & Aims: A20 is an intracellular ubiquitin-editing enzyme that plays an important role in the negative feedback regulation of NF-κB activation in response to a diverse range of stimuli. Liver ischemia/reperfusion injury is associated with rapid activation of NF-κB signaling, but the role of NF-κB in hepatic ischemia/reperfusion injury remains controversial. The NF-κB signaling pathway mediates both protective and deleterious effects in the liver. Here, we examined whether A20 inhibited or aggravated hepatic ischemia/reperfusion injury. Methods: We used IκBα super-repressor as a positive control and overexpressed A20 and IκBα super-repressor in the liver of C57BL/6 mice. Mice underwent 45min of partial hepatic ischemia and were then reperfused. Results: Protein level of A20 was increased after reperfusion. Mice subjected to ischemia/reperfusion injury showed increased NF-κB activation, as evidenced by phosphorylation of IκBα and nuclear translocation of NF-κB. Prior transfection with Ad-A20 or Ad-IκBα super-repressor attenuated NF-κB activation and aggravated liver injury. Serum aminotransferases and proinflammatory cytokines, hepatocellular necrosis, and hepatic neutrophil infiltration were markedly increased compared to those of uninfected or control virus infected mice. In addition, A20 abolished the beneficial effect of ischemic preconditioning. Conclusions: Our results suggest that inhibition of NF-κB activation by A20 aggravated partial hepatic ischemia/reperfusion injury. Understanding how the NF-κB pathway plays a role in directing a clinical outcome may lead to better prospects of more rational approaches to reduce post-ischemic liver injury.

Bacteroides fragilis의 독소 유전자 분석 및 발병기전에 관한 연구

오희복,이근영,정경태,성원근 국립보건연구원 2000 국립보건원보 Vol.37 No.-

棚素施肥水準과 刈取管理가 Alfalfa(Medicago sativa L.)의 生育과 收量및 飼料價値에 미치는 영향

金雲植,文相鎬,全炳台,李相武 建國大學校 附設 自然科學硏究所 1991 建國自然科學硏究誌 Vol.2 No.-

本 實驗은 硼砂施肥水準과 刈取管理가 알팔파의 生育과 收量 및 飼料價値에 미치는 影響을 糾明하여 실질적인 栽培技術과 利用性提高를 目的으로 硼砂施肥水準(0,15,60Kg/ha)을 主區로 하고 生育段階를 細區로(Bud, 10% bloom, 100% bloom)하여 1989년 3월∼1990년 9월까지 建國大學校 自然科學大學 附屬 實習農場內 試料圃場에서 실시한 바, 그 結果를 要約하면 다음과 같다. 1. 草長에 있어서 硼砂施肥의 效果는 나타나지 않았으나, 初年度의 1, 2次 刈取와 2次 年度의 1次 刈取에서 生育이 진행됨에 따라 높게 나타났다. 2. 硼砂施肥에 따른 B??·B₁·B₂區의 收量에는 有意差가 없었으며, 生育이 진행됨에 따라 乾物收量은 높게 나타나 有意性이 (p<0.01, 0.05) 인정되었다. 3. 粗蛋白質 및 NDF, ADF 成分에 대한 硼砂 影響은 없었으며 生育이 진행됨에 따라 粗蛋白質含量은 떨어지고 NDF, ADF含量은 높아졌다. 4. 硼砂施肥에 의한 粗蛋白質收量은 有意差가 없었으며, 生育이 진행됨에 따라 粗蛋白質收量은 높게 나타나 그 有意性이 (p<0.05) 인정되었다. 5. 硼砂施肥에 따른 嗜好性은 B??·B₁·B₂區가 같은 傾向을 보였으며, 生育段階에 따른 嗜好性은 많은 차이를 보여 初年度의 1次 刈取時 Bud >10% bloom >100% bloom期 순으로 높게 나타났다. The purpose of the experiment was carried out to determine the effects of boron application and cutting management on the growth, yield and feeding value for the purpose of practical culture and utilization techniques. The trial was performed at different borax application level(main treatments ; 0, 15, 30kg/ha) and growth stage(sub treatments : Bud, 10% bloom and 100% bloom stage) from 1989 to 1990 in experimental field college of Natural Science, Kon Kuk University. The results obtained are summarized as follows : 1. The effect of borax application in the plant length was shown, but plant length in the first and second cutting time of the first year and first cutting time of the second year after sown was high by the progression of growth. 2. There was not significant among main treatments(B??·B₁·B₂)in dry matter yield, but was shown significantly high (p<0.01, 0.05) by the progression of growth in first cutting time. 3. The content of chemical composition, NDF and ADF were not affected by boron application, and content of crude protein was decreased and those of curde fiber, NDF and ADF were increased by the progression of growht in first cutting time. 4. There was not significant among main treatments(B??·B₁·B₂) in crude protein yield but was significant (p<0.05) by the progression of growth in first cutting time. 5. There was not significant among main treatments(B??·B₁·B₂) in palatability, but palatability by growth stage was the highest at bud stage in first cutting time of first year after sown.

<i>CYP2A6</i> and <i>ERCC1</i> polymorphisms correlate with efficacy of S-1 plus cisplatin in metastatic gastric cancer patients

Park, S R,Kong, S-Y,Nam, B-H,Choi, I J,Kim, C G,Lee, J Y,Cho, S J,Kim, Y W,Ryu, K W,Lee, J H,Rhee, J,Park, Y-I,Kim, N K Nature Publishing Group 2011 The British journal of cancer Vol.104 No.7

<P><B>Background:</B></P><P>We evaluated the association between polymorphisms of cytochrome P450 2A6 (<I>CYP2A6</I>)/excision repair cross-complementation group 1 (<I>ERCC1</I>)/X-ray repair cross-complementing group 1(<I>XRCC1</I>) and treatment outcomes of metastatic gastric cancer (MGC) patients treated with S-1/cisplatin.</P><P><B>Methods:</B></P><P>Among MGC patients (<I>n</I>=108), who received S-1 (40 mg m<SUP>−2</SUP> b.i.d., days 1–14) and cisplatin (60 mg m<SUP>−2</SUP>, day 1) every 3 weeks, we analysed the wild-type allele (<I>W</I>) and variants (<I>V</I>) of <I>CYP2A6</I> (<I>*4</I>, <I>*7, *9, *10</I>), and the polymorphisms of <I>ERCC1</I> (rs11615, rs3212986) and <I>XRCC1</I> (rs25487).</P><P><B>Results:</B></P><P>Patients having fewer <I>CYP2A6</I> variants had better response rates (<I>W</I>/<I>W vs W</I>/<I>V</I> other than <I>*1/*4 vs V</I>/<I>V</I> or <I>*1/*4</I>=66.7 <I>vs</I> 58.3 <I>vs</I> 32.3% <I>P</I>=0.008), time to progression (TTP) (7.2 <I>vs</I> 6.1 <I>vs</I> 3.5 months, <I>P</I>=0.021), and overall survival (23.2 <I>vs</I> 15.4 <I>vs</I> 12.0 months, <I>P</I>=0.004). <I>ERCC1 19442C</I>><I>A</I> (rs3212986) was also associated with response rate (<I>C/C</I>, 46.7% <I>vs C/A</I>, 55.3% <I>vs A/A</I>, 87.5%) (<I>P</I>=0.048) and TTP (4.4 <I>vs</I> 7.6 <I>vs</I> 7.9 months) (<I>P</I>=0.012). Patients carrying both risk genotypes of <I>CYP2A6</I> (<I>V</I>/<I>V</I> or <I>1/*4</I>) and <I>ERCC1 19442C</I>><I>A</I> (<I>C/C</I>) <I>vs</I> those carrying none showed an adjusted odds ratio of 0.113 (<I>P</I>=0.004) for response, and adjusted hazard ratios of 3.748 (<I>P</I>=0.0001) for TTP and 2.961 (<I>P</I>=0.006) for death.</P><P><B>Conclusion:</B></P><P>Polymorphisms of <I>CYP2A6</I> and <I>ERCC1 19442C</I>><I>A</I> correlated with the efficacy of S-1/cisplatin.</P>

Multiple novel H5N6 highly pathogenic avian influenza viruses, South Korea, 2016

Lee, E.K.,Song, B.M.,Lee, Y.N.,Heo, G.B.,Bae, Y.C.,Joh, S.J.,Park, S.C.,Choi, K.S.,Lee, H.J.,Jang, I.,Kang, M.S.,Jeong, O.M.,Choi, B.K.,Lee, S.M.,Jeong, S.C.,Park, B.K.,Lee, H.S.,Lee, Y.J. Elsevier Science 2017 INFECTION GENETICS AND EVOLUTION Vol.51 No.-

<P>We report the identification of novel highly pathogenic avian influenza viruses of subtype H5N6, Glade 23.4.4, that presumably originated from China. In addition, reassortant strains with Eurasian lineage low pathogenic avian influenza viruses were isolated in wild birds and poultry in South Korea. The emergence of these novel H5N6 viruses and their circulation among bird populations are of great concern because of the potential for virus dissemination with intercontinental wild bird migration. (C) 2017 Elsevier B.V. All rights reserved.</P>

Induction of interleukin-8 production via nuclear factor-&kgr;B activation in human intestinal epithelial cells infected with <i>Vibrio vulnificus</i>

Lee, B. C.,Kim, S. H.,Choi, S. H.,Kim, T. S. Blackwell Science Ltd 2005 Immunology Vol.115 No.4

<P>Summary</P><P><I>Vibrio vulnificus</I>, a Gram-negative estuarine bacterium, is a causative agent of food-borne diseases, such as life-threatening septicaemia and wound infection disease. <I>V. vulnificus</I> penetrating into the epithelial barrier stimulates an inflammatory response in the adjacent mucosa. Therefore, interaction between <I>V. vulnificus</I> and epithelial cells is important for understanding of both the immunology of mucosal surfaces and <I>V. vulnificus</I>. In this study, we investigated the effect and action mechanism of <I>V. vulnificus</I> infection on production of interleukin (IL)-8, a proinflammatory cytokine, in human intestinal epithelial INT-407 cells. <I>V. vulnificus</I> infection significantly induced IL-8 production in a time- and multiplicity of infection (MOI)-dependent manner, as determined by human IL-8 enzyme-linked immunosorbent assay (ELISA). In addition, <I>V. vulnificus</I> infection significantly increased IL-8 mRNA levels in INT-407 cells, indicating that the increased IL-8 production by <I>V. vulnificus</I> occurred at the transcriptional level. <I>V. vulnificus</I> infection also enhanced IL-8 gene promoter activity in INT-407 cells transiently transfected with IL-8 promoter constructs, but this effect was impaired in INT-407 cells transfected with IL-8 promoter constructs deleted or mutated of a &kgr;B site. <I>V. vulnificus</I> infection increased the nuclear factor-kappaB (NF-&kgr;B) binding activity to a &kgr;B site and the degradation of I&kgr;B-&agr; protein in a time- and a MOI-dependent manner. Furthermore, BAY11-7082, an inhibitor of NF-&kgr;B activation, significantly reduced the IL-8 production, NF-&kgr;B binding activity and I&kgr;B-&agr; degradation induced by <I>V. vulnificus</I> infection. Taken together, these results indicate clearly that <I>V. vulnificus</I> infection significantly induces IL-8 production in human intestinal epithelial cells via NF-&kgr;B activation.</P>