Dual-channel separation system based on platanus fruit-like Ni@Ni(OH)2 hierarchical architecture for fast, efficient and continuous light/heavy oil–water separation

Atian Xie,Jiuyun Cui,Yangyang Chen,Jihui Lang,Chunxiang Li,Yongsheng Yan,Jiangdong Dai 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.74 No.-

Realization of fast, efficient and continuous oil–water separation is of great significance for solving theproblem of oil pollution. We present here a facile hydrothermal strategy to synthesize a superhydrophilicplatanus fruit-like Ni@Ni(OH)2 hierarchical architecture (SI-PFHA). Additionally, superhydrophobicplatanus fruit-like Ni@Ni(OH)2 hierarchical architecture (SO-PFHA) is obtained through stearic acidmodification of SI-PFHA. Dual-channel separation system is integrated based on two superwettingmembranes. Such separation system is competent for continuous light/heavy oil–water separation withhighflux and high separation efficiency. Importantly, outstanding durability and reusability make thisseparation system a promising strategy for practical application in remediation of oily wastewater.

Comments on the utilization of Mann Whitney U test and Kaplan-Meier method

Jian Xie,Lang Li 대한부인종양학회 2021 Journal of Gynecologic Oncology Vol.32 No.3

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MiR-1224-5p modulates osteogenesis by coordinating osteoblast/osteoclast differentiation via the Rap1 signaling target ADCY2

Hu Liangcong,Xie Xudong,Xue Hang,Wang Tiantian,Panayi Adriana C.,Lin Ze,Xiong Yuan,Cao Faqi,Yan Chengcheng,Chen Lang,Cheng Peng,Zha Kangkang,Sun Yun,Liu Guodong,Yu Chenyan,Hu Yiqiang,Tao Ranyang,Zhou 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

MicroRNAs (miRNAs) broadly regulate normal biological functions of bone and the progression of fracture healing and osteoporosis. Recently, it has been reported that miR-1224-5p in fracture plasma is a potential therapy for osteogenesis. To investigate the roles of miR-1224-5p and the Rap1 signaling pathway in fracture healing and osteoporosis development and progression, we used BMMs, BMSCs, and skull osteoblast precursor cells for in vitro osteogenesis and osteoclastogenesis studies. Osteoblastogenesis and osteoclastogenesis were detected by ALP, ARS, and TRAP staining and bone slice resorption pit assays. The miR-1224-5p target gene was assessed by siRNA-mediated target gene knockdown and luciferase reporter assays. To explore the Rap1 pathway, we performed high-throughput sequencing, western blotting, RT-PCR, chromatin immunoprecipitation assays and immunohistochemical staining. In vivo, bone healing was judged by the cortical femoral defect, cranial bone defect and femoral fracture models. Progression of osteoporosis was evaluated by an ovariectomy model and an aged osteoporosis model. We discovered that the expression of miR-1224-5p was positively correlated with fracture healing progression. Moreover, in vitro, overexpression of miR-1224-5p slowed Rankl-induced osteoclast differentiation and promoted osteoblast differentiation via the Rap1-signaling pathway by targeting ADCY2. In addition, in vivo overexpression of miR-1224-5p significantly promoted fracture healing and ameliorated the progression of osteoporosis caused by estrogen deficiency or aging. Furthermore, knockdown of miRNA-1224-5p inhibited bone regeneration in mice and accelerated the progression of osteoporosis in elderly mice. Taken together, these results identify miR-1224-5p as a key bone osteogenic regulator, which may be a potential therapeutic target for osteoporosis and fracture nonunion.

Systematic Modulation of Gelation Dynamics of Snakehead (Channa argus) Skin Collagen by Environmental Parameters

Juntao Zhang,Benmei Wei,Lang He,Chengzhi Xu,Dong Xie,Kyung-Wook Paik,Haibo Wang 한국고분자학회 2017 Macromolecular Research Vol.25 No.11

Gel matrices of mammalian collagen are routinely used as bioengineering materials and food thickening agents, for which the modulation of their mechanical properties is a key issue. However, little information is available regarding gel matrices based on fish-sourced collagen, which offer unique advantages for some applications. Here, a rheology assay, which is the most commonly used method for monitoring developing gels, was used to systematically reveal the influence of environmental parameters on the gelation dynamics and rheological properties of pepsin-soluble collagen (PSC) extracted from snakehead (Channa argus) skins. The gelation dynamics and equilibrium elastic moduli of PSC were affected by concentration, temperature, pH, buffer, and ion strength and type. SEM and TEM images of gels at different concentrations and incubation temperatures confirmed that the mechanical properties of PSC gels are directly related to the density, rather than size, of fibrils. Additionally, the relationship between the biological and mechanical properties of these collagen gels was also evaluated. The present study would facilitate a better understanding of the gelation of fish-sourced collagen and enable more precise control of the mechanical properties of these gel matrices.

Modulation of the Self-Assembly of Collagen by Phytic Acid: An In Vitro Study

Xiao Tu,Xincheng Chen,Ying Peng,Jie Nan,Benmei Wei,Lang He,Chengzhi Xu,Yuling Xu,Dong Xie,Juntao Zhang,Haibo Wang 한국고분자학회 2018 Macromolecular Research Vol.26 No.13

Phytic acid, containing a myoinositol ring coupled with six phosphate groups, can react with the amino groups of collagen to regulate their self-assembly behavior. The aim of this research is to evaluate the effects of phytic acid on the selfassembly behavior of collagen, the structures and properties of the resulting fibrils and hydrogels. Turbidity and chloramine T assay suggested that phytic acid could improve the self-assembly kinetics and degree of collagen, and the optimal ratio of phytic acid/collagen was 1/1 (w/w). Scanning electron microscopy (SEM) analysis indicated that co-fibrils of collagen with phytic acid are more slender than that of pure collagen, and transmission electron microscopy (TEM) reveals that the characteristic D-periodicity of collagen fibrils is not affected by phytic acid. Besides, differential scanning calorimetry (DSC) and rheology revealed that the thermal stability of collagen fibrils and the viscoelasticity of collagen hydrogels could be improved by phytic acid and the optimal ratio of phytic acid/collagen is 1/1 (w/w).

Long Noncoding RNA HEIH Promotes Colorectal Cancer Tumorigenesis via Counteracting miR-939Mediated Transcriptional Repression of Bcl-xL

Chunhui Cui,Duanyang Zhai,Lianxu Cai,Qiaobin Duan,Lang Xie,Jinlong Yu 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3

Purpose Studies have found that long noncoding RNA HEIH (lncRNA-HEIH) is upregulated and facilitates hepatocellular carcinoma tumor growth. However, its clinical significances, roles, and action mechanism in colorectal cancer (CRC) remains unidentified. Materials and Methods lncRNA-HEIH expression in CRC tissues and cell lines was measured by quantitative realtime polymerase chain reaction. Cell Counting Kit-8, ethynyl deoxyuridine incorporation assay, terminal deoxynucleotidyl transferase dUTP nick end labeling staining, and nude mice xenografts assays were performed to investigate the roles of lncRNA-HEIH. RNA pull-down, RNA immunoprecipitation, chromatin immunoprecipitation, and luciferase reporter assays were performed to investigate the action mechanisms of lncRNA-HEIH. Results In this study, we found that lncRNA-HEIH is significantly increased in CRC tissues and cell lines. lncRNA-HEIH expression is positively associated with tumor size, invasion depth, and poor prognosis of CRC patients. Enhanced expression of lncRNA-HEIH promotes CRC cell proliferation and decreases apoptosis in vitro, and promotes CRC tumor growth in vivo. Whereas knockdown of lncRNA-HEIH inhibits CRC cell proliferation and induces apoptosis in vitro, and suppresses CRC tumor growth in vivo. Mechanistically, lncRNA-HEIH physically binds to miR-939. The interaction between lncRNA-HEIH and miR-939 damages the binding between miR-939 and nuclear factor B (NF-B), increases the binding of NF-B to Bcl-xL promoter, and promotes the transcription and expression of Bcl-xL. Moreover, Bcl-xL expression is positively associated with lncRNA-HEIH in CRC tissues. Blocking the interaction between lncRNA-HEIH and miR-939 abolishes the effects of lncRNA-HEIH on CRC tumorigenesis. Conclusion This study demonstrated that lncRNA-HEIH promotes CRC tumorigenesis through counteracting miR-939mediated transcriptional repression of Bcl-xL, and suggested that lncRNAHEIH may serve as a prognostic biomarker and therapeutic target for CRC.

Improving the interfacial stability, conductivity, and electrochemical performance of Li2MoO3@g-C3N4 composite as a promising cathode for lithium-ion battery

Zhi-Xin He,Hai-Tao Yu,Fei He,Ying Xie,Lang Yuan,Ting-Feng Yi 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.119 No.-

Li-rich layered Li2MoO3 (LMO) materials are one promising cathode materials for Li-ion batteries due totheir high theoretical capacity and without oxygen evolution. However, the poor electrical conductivityand air instability have limited its application as a cathode material for lithium-ion battery. To solve theseproblems, Li2MoO3/g-C3N4 composites were successfully constructed by combining the molten salt andball milling methods. Carbon nitride (g-C3N4) with an abundant nitrogen-containing carbon frameworkcontains a large number of ‘‘hole” defects and double-bonded nitrogen vacancy edges, which are favorablefor the adsorption and diffusion of Li ions. In addition, density functional theory (DFT) calculationsrevealed that a stable interface can be formed between g-C3N4 and LMO, which also leads to the improvementof the electronic conductivity and the reduction of interfacial impedance of the composite. Therefore, the electrochemical performance of the composite material is significantly improved. The dischargecapacity of GLMO-5 at a current density of 1700 mA g1 is 64.6 mAh/g, which is much greater thanthe value (29.9 mAh/g) of the original LMO sample under the same conditions. EIS further shows thatGLMO-5 has the highest discharge capacity with a DLi+ value of 1.94 1014 cm2 s1. These results indicatethat constructing LMO-based composites with a highly stable layered material containing unsaturatedfunctional groups should be an effective strategy to enhance the interfacial stability, electronicconductivity, and thus the electrochemical performances of the cathode materials.