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      • SCIESCOPUSKCI등재

        충적과정중 미국인삼 종자의 생리 및 생화하적 지표에 관한 동태학적 연구 III

        Huang, Yao-Ge,Li, Xiang-Gao,Kuang, Ya-Lan,Yan, Jie-Kun,Cui, Shu-Yu,Yu, Wen-Bo,Yang, Ji-Xiang,Liu, Ren-Song,Kim, Hack-Seang 고려인삼학회 1997 Journal of Ginseng Research Vol.21 No.1

        Dynamic parameters of biochemistry including the POD (peroxidase) activity, contents of DNA and RNA, isozymes of POD and ES (esterase) in American send (Panax quinquefolium L.) seed are reported in the present paper. The dynamic changes of POD activity proved that the PAS (physiological afterripening stage) is a stage in which some substances are prepared for seed germination. The POD activity correlated with ER (embryo ratio) significantly DNA content changed little only within 0.0036∼0.013 mg/ml, which did not correlate with ER. RNA content changed from 0.1539 to 1.0313 mg/ml and correlated significantly with RE during all of the embryo afterripening. None of the POD isozyme band was obtained in ESGS (embryo slowly growth stage), but five bands in ERGS (embryo rapidly growth stage) and six bands in PAS. Four bands of ES isozymes were obtained in ESGS, but six bands in ERGS, particularly, the content of ES isozymes increased in PAS. All of these may provide some information for understanding the dormancy mechanisms of American song seed.

      • KCI등재

        Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites

        Lan-Qing Ma,Yan-Wu Guo,Hui-Li Guo,Xing Li,Li-Li Huang,Bo-Ning Zhang,Xiao-Bin Pang,Ben-Ye Liu,Hong Wang 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3

        In our recent work (Ma et al., in Planta229(3):457–469, 2009a and 229(4):1077–1086, 2009b),two three-intron type III PKS genes, PcPKS1 and PcPKS2,were isolated from Polygonum cuspidatum Sieb. et Zucc. Phylogenetic and functional analyses revealed PcPKS1 is athree-intron chalcone synthase (CHS) gene, and PcPKS2 isfound to be a three-intron benzalacetone synthase (BAS)gene. The regular CHS encoded by a single intron genehave not been isolated and characterized from P. cuspidatum. In this work a further CHS with one intron (PcPKS3)and a stilbene synthase (STS) gene with three-intron(PcPKS5) were isolated and characterized by functionaland phylogenetic analyses. In comparison with PcPKS1, abifunctional enzyme with both CHS and BAS activity, theenzymatic product of recombinant PcPKS3 was naringenin,bis-noryangonin (BNY) and 4-coumaroyltriacetic acidlactone (CTAL) occurred as side products. The PcPKS5synthesized resveratrol and a trace amount of naringeninfrom p-coumaroyl-CoA. To our knowledge, PcPKS5 is thefirst reported three-intron STS gene in flowering plants. Inthis work, we speculated that this involved a possibleevolutionary route of plant-specific type III PKS superfamilyin P. cuspidatum.

      • SCIESCOPUSKCI등재

        REVERSIBLE AND PSEUDO-REVERSIBLE RINGS

        Huang, Juan,Jin, Hai-lan,Lee, Yang,Piao, Zhelin Korean Mathematical Society 2019 대한수학회보 Vol.56 No.5

        This article concerns the structure of idempotents in reversible and pseudo-reversible rings in relation with various sorts of ring extensions. It is known that a ring R is reversible if and only if $ab{\in}I(R)$ for $a,b{\in}R$ implies ab = ba; and a ring R shall be said to be pseudoreversible if $0{\neq}ab{\in}I(R)$ for $a,b{\in}R$ implies ab = ba, where I(R) is the set of all idempotents in R. Pseudo-reversible is seated between reversible and quasi-reversible. It is proved that the reversibility, pseudoreversibility, and quasi-reversibility are equivalent in Dorroh extensions and direct products. Dorroh extensions are also used to construct several sorts of rings which are necessary in the process.

      • KCI등재

        Restorative Justice in Taiwan : An Overview

        Huang, Lan Ying 梨花女子大學校 法學硏究所 2010 法學論集 Vol.15 No.1

        대만의 법무부가 이제 막 처음으로 “회복적 사법 프로그램”의 도입에 착수하고 있음에도 불구하고, 2000년 이후 이 계획은 공적 담론과 학계 연구의 중심에 있어 왔다. 이러한 회복적 사법 촉구의 배경에는 다음과 같은 것들이 있다. 전통적으로, 식민지배 이후 대만 사회는 공식적이고 투명한 사법기관들을 비공식적인 기관들이 대체하는 ‘안으로는 유교적, 밖으로는 법률 존중주의적’ 사회로 묘사되어 왔다. 대만 사람들에게는 대체적 분쟁해결수단을 널리 활용해 온 오랜 역사가 있으며, 얼마 전부터 정부는 사법적 인권을 고취시키자는 압력도 계속 받고 있다. ‘당사자주의’의 도입은 피해자의 권리 보장에 효과적이지 않은 것처럼 여겨진다. 사형제도 폐지라는 논란이 많은 이슈도 피해자 운동의 또 다른 흐름이다. 복수의 목적을 달성하기 위한 형사사법제도의 개혁은 정부의 우선적인 과제 중 하나다. 이 논문에서는 회복적 사법의 세 가지 실용적인 유형에 대하여 언급하고자 한다: 소년사법제도, 참여식 사법, 그리고 형사사건에 있어서의 중재이다. 끝으로 필자는 2000년 법무부가 추진하였던 회복적 사법 프로그램의 배경에 대하여 살펴보기 위하여, 현재의 법적 구조에 대하여 비판적으로 검토해 보고자 한다. Although the ministry of justice in Taiwan is just beginning to initiate the first “restorative justice programme”, the idea has been the focus of both official discourse and academic investigation since 2000. There are a few forces behind this newly emerged restorative justice urge. Traditionally, post colonization Taiwan society is described as an ‘inwardly Confucian, outwardly legalistic’ (內儒外法) society, in which informal institutions preempted the transplanted formal legal institutions. The Taiwan people have a long history of using alternative dispute resolution mechanism. More recently, the government is under increasing pressure of promoting judicial human right. The introduction of adversary system can not be deemed beneficial of promoting victim’s right. The controversial issue of abolishing death penalty also rises another wave of victim movement. The reform of criminal justice system to serve multiple purposes has become one of the prioritized government agenda. In this article, I describe three practical forms of restorative justice: juvenile justice system (少年司法制度), participatory justice (參與式司法), and mediation service for criminal cases (刑事案件調解). In the end, I critically review the current legal structure to address the background of the 2010 restorative justice programme under Ministry of Justice (法務部修復式司法試行方案).

      • SCIESCOPUSKCI등재

        충적과정중 미국인삼 종자의 생리 및 생화학적 지표에 관한 동태학적 연구 I

        Huang, Yao-Ge,Li, Xiang-Gao,Cui, Shu-Yu,Yu, Wen-Bo,Kuang, Ya-Lan,Yan, Jie-kun,Yang, Ji-Xiang,Liu, Ren-Song,Kim, Hack-Seang The Korean Society of Ginseng 1997 Journal of Ginseng Research Vol.21 No.1

        Dynamic parameters of biochemistry including the contents of soluble carbohydrate (SC) , crude fat (CF) , fatty arid (FA) and soluble protein (SP) in American send (Panax qufnquelolium L.) seed are reported in the present paper. When embryo ratio (ER) increased from 7.31 to 20.48%, the SC content decreased from 4.08 to 1.16%. After that, SC content changed little. The CF content decreased successively from 34.50 to 14.40% from the beginning to the end during the embryo afterripening. The changes of FA content did not correlate with that of ER and the changing range of SP content was not large during the seed stratification. All of these may provide some information for understanding the dormancy mechanisms of American seng seed.

      • SCIESCOPUSKCI등재

        Dynamic Studies on Physiology and Biochemistry in American Seng Seed During Stratification - Part I. Embryo Ratio, Dry Weight Ratio and respiration Rate

        Huang, Yao-Ge,Li, Xiang-Gao,Yang, Ji-Xiang,Kuang, Ya-Lan,Yan, Jie-Kun,Cui, Shu-Yu,Liu, Ren-Song,Kim, Hack-Seang The Korean Society of Ginseng 1996 Journal of Ginseng Research Vol.20 No.3

        Dynamic parameters of physiology including embryo ratio (ER), embryo length to endosperm length, dry weight ratio between embryo and endosperm (DWR) and respiration rate (RR) in American send (Panax quinquefolium L.) seed were investigated. According to the changes of ER during seed stratification, the duration of embryo afterripening could be divided into three stages as \circled1 embryo slow growth stage (ESGS), ER increased from 7.31% to 20.48% (0.16% day-1): \circled2 embryo rapid growth stage (ERGS), ER increased to 80.98% (0.61% day-1) (75G5+ ERGS=morphological afterripening stage (MP,5)) and \circled3pysiological afterripening stage (PAS), ER Increased to 88.50% (0.094 day-1) only. DWR Increased slowly from 0.20% to 2.76clp (0.016% day-1) in MAS and rapidly to 8.81% (0.061% day-1) in PAS. The RR correlated significantly with ER as well (r=0.8934 > rot, 0.6610). The steep increment of both DWR and RR in PAS indicated that the PAS was not a static stage although the ER was not changed too much. All of these may provide some information for understanding the dormancy mechanisms of American sting seed.

      • KCI등재후보
      • KCI등재

        Molecular Cloning and Characterization of clyA Genes in Various Serotypes of Salmonella enterica

        Lan Ji Huang,Jinghua Cui,Hong Hua Piao,홍영진,최현일,류필열 한국미생물학회 2010 The journal of microbiology Vol.48 No.5

        Cytolysin A (ClyA) is a pore-forming hemolytic protein encoded by the clyA gene. It has been identified in Salmonella enterica serovars Typhi and Paratyphi A. To identify and characterize the clyA genes in various Salmonella enterica strains, 21 different serotypes of strains isolated from clinical specimens were presently examined. Full-length clyA genes were found in S. enterica serovar Brandenburg, Indiana, Panama, and Schwarzengrund strains by polymerase chain reaction amplification. The ClyA proteins from these four strains showed >97% amino acid identity to that of S. enterica serovar Typhi. Although all four serovars expressed detectable levels of ClyA as determined by Western blot analysis, they did not show a strong hemolytic effect on blood agar, indicating that ClyA may not be efficiently expressed or secreted. Escherichia coli transformed with clyA genes from the four serovars enhanced production of ClyA proteins and hemolytic activities to a level similar to S. enterica serovar Typhi ClyA. The present results suggest that ClyA may play a role in the pathogenesis of S. enterica serovar Brandenburg, Indiana, Panama and Schwarzengrund.

      • Effects of post anneal for the INZO films prepared by ultrasonic spray pyrolysis

        Lan, Wen-How,Li, Yue-Lin,Chung, Yu-Chieh,Yu, Cheng-Chang,Chou, Yi-Chun,Wu, Yi-Da,Huang, Kai-Feng,Chen, Lung-Chien Techno-Press 2014 Advances in nano research Vol.2 No.4

        Indium-nitrogen co-doped zinc oxide thin films (INZO) were prepared on glass substrates in the atmosphere by ultrasonic spray pyrolysis. The aqueous solution of zinc acetate, ammonium acetate and different indium sources: indium (III) chloride and indium (III) nitrate were used as the precursors. After film deposition, different anneal temperature treatment as 350, 450, $550^{\circ}C$ were applied. Electrical properties as concentration and mobility were characterized by Hall measurement. The surface morphology and crystalline quality were characterized by SEM and XRD. With the activation energy analysis for both films, the concentration variation of the films at different heat treatment temperature was realized. Donors correspond to zinc related states dominate the conduction mechanism for these INZO films after $550^{\circ}C$ high temperature heat treatment process.

      • KCI등재

        Effect of Sildenafil on Neuropathic Pain and Hemodynamics in Rats

        Lan Ji Huang,최정일,김웅모,이형곤,김여옥,윤명하 연세대학교의과대학 2010 Yonsei medical journal Vol.51 No.1

        Purpose: The inhibition of phosphodiesterase 5 produces an antinociception through the increase of cyclic guanosine monophosphate (cGMP), and increasing cGMP levels enhance the release of γ-aminobutyric acid (GABA). Furthermore, this phosphodiesterase 5 plays a pivotal role in the regulation of the vasodilatation associated to cGMP. In this work, we examined the contribution of GABA receptors to the effect of sildenafil, a phosphodiesterase 5 inhibitor, in a neuropathic pain rat, and assessed the hemodynamic effect of sildenafil in normal rats. Materials and Methods: Neuropathic pain was induced by ligation of L5/6 spinal nerves in Sprague-Dawley male rats. After observing the effect of intravenous sildenafil on neuropathic pain, GABAA receptor antagonist (bicuculline) and GABAB receptor antagonist (saclofen) were administered prior to delivery of sildenafil to determine the role of GABA receptors in the activity of sildenafil. For hemodynamic measurements, catheters were inserted into the tail artery. Mean arterial pressure (MAP) and heart rate (HR) were measured over 60 min following administration of sildenafil. Results: Intravenous sildenafil dose-dependently increased the withdrawal threshold to the von Frey filament application in the ligated paw. Intravenous bicuculline and saclofen reversed the antinociception of sildenafil. Intravenous sildenafil increased the magnitude of MAP reduction at the maximal dosage, but it did not affect HR response. Conclusion: These results suggest that sildenafil is active in causing neuropathic pain. Both GABAA and GABAB receptors are involved in the antinociceptive effect of sildenafil. Additionally, intravenous sildenafil reduces MAP without affecting HR.

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