http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Lee, Jeung-Hoon,Kye, Kyung-Chae,Seo, Eun-Young,Lee, Kyungmoon,Lee, Sang-Keun,Lim, Jong-Soon,Seo, Young-Joon,Kim, Chang Deok,Park, Jang-Kyu The Korean Academy of Medical Sciences 2008 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.23 No.2
<P>In a previous search for the differentially expressed genes in keratinocyte differentiation, we identified neutrophil gelatinase-associated lipocalin (NGAL) as a calcium-induced gene. In this study, we further verified the expression of NGAL in cultured keratinocytes as well as in several skin diseases. Reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and ELISA clearly showed that NGAL expression was markedly increased in calcium-induced keratinocyte differentiation in vitro. However, in our previous report, NGAL expression was not detected in normal skin tissue except for hair follicle by in situ hybridization and immunohistochemistry, indicating the difference of cell status between in vitro and in vitro conditions. Interestingly, NGAL expression was highly increased in psoriasis-like inflammatory disorders (lichen planus and pityriasis rubura pilaris) and skin cancers (keratoacanthoma and squamous cell carcinoma), implying that NGAL may be related with the epidermal hyperplasia. Collectively, these results reveal the potential importance of NGAL in the maintenance of skin homeostasis.</P>
Role of protein kinase C delta in X-ray-induced apoptosis of keratinocyte
Lee, Young-Sook,Sohn, Kyung-Cheol,Kim, Ki-Hwan,Cho, Moon-June,Hur, Gang Min,Yoon, Tae-Jin,Kim, Sung Kyu,Lee, Kyungmoon,Lee, Jeung-Hoon,Kim, Chang Deok Blackwell Publishing Ltd 2009 Experimental dermatology Vol.18 No.1
<P>Abstract: </P><P>In this study, we investigated the process of X-ray-induced apoptosis of skin keratinocyte, and the functional role of protein kinase C delta (PKC&dgr;) and downstream signalling cascade. High-dose X-ray irradiation (10 Gy) led to the apoptosis of HaCaT keratinocyte, accompanied by PKC&dgr; cleavage. Treatment with PKC&dgr; inhibitor and adenoviral transduction of dominant-negative PKC&dgr; clearly inhibited the X-ray-induced apoptosis of keratinocyte. In addition, X-ray induced the phosphorylation of extracellular signal-regulated kinases 1/2 (ERK1/2) and inhibition by ERK1/2 inhibitor abrogated the X-ray-induced apoptosis. Interestingly, overexpression of dominant-negative PKC&dgr; markedly blocked the X-ray-induced phosphorylation of ERK1/2, suggesting that ERK1/2 is the functional downstream effector of PKC&dgr;. Next, we investigated the difference between UVB and X-ray response. UVB induced the apoptosis of keratinocyte in a PKC&dgr;-dependent manner, similar to X-ray response. However, UVB irradiation induced the phosphorylation of c-jun N-terminal kinases (JNK) and inhibition of JNK significantly protected the UVB-induced apoptosis. These results demonstrate that PKC&dgr; is a key regulator in X-ray-induced apoptosis of keratinocyte and suggest that there is subtle difference in downstream signalling cascade between UVB and X-ray response of keratinocyte.</P>
Lee, Soojin,Kim, Borim,Park, Kyungmoon,Um, Youngsoon,Lee, Jinwon Humana Press 2012 Applied biochemistry and biotechnology Vol.166 No.7
<P>meso-2,3-Butanediol (meso-2,3-BDO) is essential for the synthesis of various economically valuable biosynthetic products; however, the production of meso-2,3-BDO from expensive carbon sources is an obstacle for industrial applications. In this study, genes involved in the synthesis of 2,3-BDO in Klebsiella pneumoniae were identified and used to genetically modify Escherichia coli for meso-2,3-BDO production. Two 2,3-BDO biosynthesis genes-budA, encoding acetolactate, and meso-budC, encoding meso-SADH-from K. pneumoniae were cloned into the pUC18 plasmid and introduced into E. coli. In 2??l batch culture, the SGSB03 E. coli strain yielded meso-2,3-BDO at 0.31??g/g(glucose) (with a maximum of 15.7??g/l(culture) after 48??h) and 0.21??g/g(crude glycerol) (with a maximum of 6.9??g/l(culture) after 48??h). Batch cultures were grown under optimized conditions (aerobic, 6% carbon source, 37??°C, and initial pH 7). To find the optimal culture conditions for meso-2,3-BDO production, we evaluated the enzyme activity of meso-SADH and the whole cell conversion yield (meso-2,3-BDO/acetoin) of the E. coli SGSB02, which contains pSB02. meso-SADH showed high enzyme activity at 30-37??°C and pH 7 (30.5-41.5 U/mg of protein), and the conversion yield of SGSB02 E. coli was highest at 37-42??°C and a pH of 7 (0.25-0.28 g( meso-2,3-BDO)/g(acetoin)).</P>
( Sol Lee Park ),( Jang Yeon Cho ),( Su Hyun Kim ),( Hong-ju Lee ),( Sang Hyun Kim ),( Min Ju Suh ),( Sion Ham ),( Shashi Kant Bhatia ),( Ranjit Gurav ),( See-hyoung Park ),( Kyungmoon Park ),( Yun-go 한국미생물 · 생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.1
Ever since bioplastics were globally introduced to a wide range of industries, the disposal of used products made with bioplastics has become an issue inseparable from their application. Unlike petroleum-based plastics, bioplastics can be completely decomposed into water and carbon dioxide by microorganisms in a relatively short time, which is an advantage. However, there is little information on the specific degraders and accelerating factors for biodegradation. To elucidate a new strain for biodegrading poly-3-hydroxybutyrate (PHB), we screened out one PHB-degrading bacterium, Microbulbifer sp. SOL03, which is the first reported strain from the Microbulbifer genus to show PHB degradation activity, although Microbulbifer species are known to be complex carbohydrate degraders found in high-salt environments. In this study, we evaluated its biodegradability using solid- and liquid-based methods in addition to examining the changes in physical properties throughout the biodegradation process. Furthermore, we established the optimal conditions for biodegradation with respect to temperature, salt concentration, and additional carbon and nitrogen sources; accordingly, a temperature of 37℃ with the addition of 3% NaCl without additional carbon sources, was determined to be optimal. In summary, we found that Microbulbifer sp. SOL03 showed a PHB degradation yield of almost 97% after 10 days. To the best of our knowledge, this is the first study to investigate the potent bioplastic degradation activity of Microbulbifer sp., and we believe that it can contribute to the development of bioplastics from application to disposal.
Jeung Hoon Lee,Kyung Chae Kye,서은영,Kyungmoon Lee,Sang Keun Lee,Jong Soon Lim,Young Joon Seo,김창덕,Jang Kyu Park 대한의학회 2008 Journal of Korean medical science Vol.23 No.2
In a previous search for the differentially expressed genes in keratinocyte differentiation, we identified neutrophil gelatinase-associated lipocalin (NGAL) as a calcium- induced gene. In this study, we further verified the expression of NGAL in cultured keratinocytes as well as in several skin diseases. Reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and ELISA clearly showed that NGAL expression was markedly increased in calcium-induced keratinocyte differentiation in vitro. However, in our previous report, NGAL expression was not detected in normal skin tissue except for hair follicle by in situ hybridization and immunohistochemistry, indicating the difference of cell status between in vitro and in vitro conditions. Interestingly, NGAL expression was highly increased in psoriasis-like inflammatory disorders (lichen planus and pityriasis rubura pilaris) and skin cancers (keratoacanthoma and squamous cell carcinoma), implying that NGAL may be related with the epidermal hyperplasia. Collectively, these results reveal the potential importance of NGAL in the maintenance of skin homeostasis.
Kim, JaeHwang,Nam, DongHoon,Lee, HooDam,Lee, KyungMoon,Lee, TaeGyu,Park, HoonMo,Lee, Jongkook JAPAN INSTITUTE OF METALS 2016 MATERIALS TRANSACTIONS Vol.57 No.2
<P>Al-Si based alloys are used to automobile parts for weight reduction. The engine block and chain cover were fabricated using the ADC12 and newly developed ADC12-M1 alloys through the high pressure die-casting. The ADC12-M1 alloy was fabricated by the addition of titanium and boron into the ADC12 alloy. Computational science such as JMatPro and Thermo-Calc was utilized in order to estimate the solidification behavior since the mechanical property of as-cast material is affected by the solidification behavior. The ADC12 and newly developed ADC12-M1 alloys belong to eutectic and hypo-eutectic alloys calculated by Thermo-Calc software, respectively. The cooling curves with the different cooling rates, fraction liquid and latent heat generation are estimated using JMatPro based on the Al-Si binary alloys in order to understand the solidification behavior between the eutectic and hypo-eutectic chemical composition. The cooling curves, fraction liquid and latent heat generation are more sensitive in the hypo-eutectic chemical composition than that in the eutectic one. The eutectic chemical composition is shifted into the higher concentration of silicon by the titanium and boron additions, resulting in that the higher difference of the yield strength is obtained in the hypo-eutectic ADC12-M1 alloy than that in the eutectic ADC12 alloy.</P>
Photochromic Mechanism in a-WO3 Thin Films Based on Raman Spectroscopic Studies
Kyungmoon Kim,Chungwon Seo,정현식,Se-Hee Lee 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.48 No.6
The photochromic mechanism in a-WO3 thin films prepared by thermal evaporation has been investigated using Raman scattering measurements. The Raman spectra of uv-irradiated thin films show a weak peak at 450 cm.1. This peak is known to be related to the gasochromic and electochromic coloration and is attributed to the formation of W5+ = O bonds. The I-V characteristic of uv-irradiated a-WO3 thin films was measured to study the change in the electrical properties due to photochromic coloration. The uv-irradiated films show enhanced conductivity due to excited carriers.
A Parametric Study on Ethanol Production from Xylose by Pichia stipitis
Lee Tae-Young,Kim Myoung-Dong,Kim Kyu-Yong,Park Kyungmoon,Ryu Yeon-Woo,Seo Jin-Ho The Korean Society for Biotechnology and Bioengine 2000 Biotechnology and Bioprocess Engineering Vol.5 No.1
Characteristics of ethanol production by a xylose-fermenting yeast, Pichia stipitis Y-7124, were studied. The sugar consumption rate and specific growth rate were higher in the glucose-containing medium than in the xylose-containing medium. Specific activities of xylose reductase and xylitol dehydrogenase were higher in the medium with xylose than glucose, suggesting their induction by xylose. Maximum specific growth rate and ethanol yield were achieved at 30 g xylose/L concentration without formation of by-products such as xylitol and acetic acid whereas a maximum ethanol concentration was obtained at 130 g/L xylose. Adding a respiratory inhibitor, rotenone, increased a maximum ethanol concentration by $10\%$ compared with the control experiment. In order to evaluate the pattern of ethanol inhibition on specific growth rate, a kinetic model based on Luong's equations was applied. The relationship between ethanol concentration and specific growth rate was hyperbolic for glucose and parabolic for xylose. A maximum ethanol concentration at which cells did not grow was 33.6 g/L for glucose and 44.7 g/L for xylose.