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이은경,김교한,강인규 대한치과기재학회 2001 대한치과재료학회지 Vol.28 No.1
The purpose of this study was to compare the shear bonding strength and the interfacial micromorpholoy of the 5th and 6th generation dentin bonding system. The evaluation of durability was performed by 1,000 thermocycling with 4℃ and 60℃ water. As the results, mean shear bond strengths of 5th generation dentin bonding systems ranged from 12.48 MPa to 16.71 MPa for One Step and OptiBond Solo, respectively. Mean shear bond strengths of 6th generation dentin bonding systems ranged from 19.03 for Prompt L-Pop to 20.12 MPa for One-Up Bond F. After the thermocycling, the shear bond strengths of 5th generation dentin bonding systems reduced more than that of the 6th generation dentin bonding system. In the thermocycle 0 and 1,000 groups, a significantly different bond strength was observed. The thickness of Hybrid layers were 2∼3㎛ and 1∼2㎛ for 5th and 6th generation dentin bonding systems, respectively. In the 5th generation dentin bonding systems, resin tags and resin penetration through lateral branches of tubules were prominent. In 6th generation, however, the same effect was not observed in the tubules.
유화 Chlorosulfonated Polyethylene Rubber (CSM)의 제조 및 특성 연구
이은경,최교창,최세영 한국고무학회 2005 엘라스토머 및 콤포지트 Vol.40 No.1
본 연구에서는 CSM 에멀젼의 물성을 향상시키고자 금속성 가교제인 magnesium carbonate 및 calcium hydroxide를 첨가하여 가교밀도, 열적특성, 표면자유에너지 그리고 인장강도, 파단신율 및 인열강도 특성을 고찰하였다, 금속성 가교제인 magnesium carbonate 및 calcium hydroxide 양이 증가함에 따라 CSM 에멀젼 필름은 가교밀도는 증가하였고, 이에 내수성과 T_(g) 값도 증가하였다. 금속성 가교제로 magnesium carbonate를 첨가하였을 때 calcium hydroxide에 비해서 다소 높은 가교밀도와 T_(g) 값을 보였다. 하지만 CSM 에멀젼 필름의 표면에너지 및 기계적 특성들은 다소 다른거동을 보였다 Magnesium carbonate 0.75% 그리고 calcium hydroxide 1.0% 첨가한 경우가 가장 높은 표면 자유에너지 값과 인장강도 및 인열강도를 보였으나, 그 이상의 양을 첨가하였을 경우에는 오히려 감소함을 확인하였다. 그러므로 본 연구에서 CSM 에멀젼의 물성을 향상시키는데 적용되는 금속성 가교제로서 calcium hydroxide 보다 magnesium carbonate가 더 적당하며, 0.75% 첨가하였을 때 보다 향상된 물성을 얻을 수 있었다. In this work. magnesium carbonate and calcium hydroxide as metallic crosslinking agent were added to chlorosulfonated polyethylene rubber (CSM) emulsion to enhance the mechanical properties of emulsion film such as tensile strength. elongation at break, and tear strength and crosslinking density. thermal features, and surface energy were also investigated. Crosslinking density of the CSM emulsion film with increasing the amount of magnesium carbonate and calcium hydroxide increased. leading to the enhancement of water resistance. It was shown that compared with calcium hydroxide, magnesium carbonate had a little higher crosslinking density and To value. The surface energy and mechanical characteristics of the CSM emulsion film, however, showed somewhat different behaviors. The highest surface energy. tensile strength. and tear strength were observed when 0.75° o for magnesium carbonate and 1.0° o for calcium hydroxide were added respectively. Therefore. it can be concluded that as metallic crosslinking agent to improve water resistance and mechanical properties of the CSM emulsion, magnesium carbonate is more preferable to calcium hydroxide
이은영,김교한 대한치과기재학회 1998 대한치과재료학회지 Vol.25 No.3
To evaluate the residual stress in porcelain surfaces by three different pressures, fracture toughness by indentation microfracture method, and shear bond strength of bonded porcelain-resin specimens were measured. The values of residual stress calculated from fracture toughness determined by the indentation microfracture method were increased with the increasing of sandblasting pressure of 7 ㎏f/㎠ showed the lowest shear bond strength, but there were no significant difference among the three groups at the 95% confidence level. It was observed that the surface condition was irregular and sharp. The experimental group sandblasted by the pressure of 3 ㎏f/㎠ showed mostly a separation between porcelain-resin interface with crack propagation inside the porcelain, while the experimental group sandblasted by the pressure of 7 ㎏f/㎠ showed porcelain cohesive fractures. As a result, the residual stress was generated by sandblasting in the porcelain surface, and this residual stress seemed to determine the failure types of bonded porcelain-resin specimens.
농작물 해충 및 진균류 방제를 위한 방선균의 분리 및 동정
이은정,강경돈,황교열,김두호,김신덕,성수일 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.1
Twenty seven out of ca. 5,000 actinomycete strains, which were isolated from soil collected throughout the country, showed antimicrobial effects against fungi, Rhizopus stronifer (ATCC6227a), Rhizoctonia solani (KCCM 11271) and yeast, Candida albicans(ATCC10231). From these antifungal microorganisms, we further selected seven strains which seemed to produce insecticidal substances with in vivo test, using silkworm, Bombyx mori and beet armyworm, Spodoptera exigua. Morphological and biochemical experiments revealed that three strains out of seven were streptomyces. Further investigations on the physical and chemical properties of these antifungal and insecticidal substances are now in progress.
Barbituric acid 개시계 레진의 치질 접착성에 미치는 가교제의 영향
나환욱,이은경,김교한,강인규 대한치과기재학회 2001 대한치과재료학회지 Vol.28 No.2
Degree of polymerization, surface hardness and tensile bond strength of the bonding resin were carried out to examine the effect of crosslinking agent, triethylene glycol dimethacrylate(TEGDMA), on the adhesion of barbituric acid-initiated resin to dentin. As the results, degree of conversion and surface hardness increased with increasing the concentration of TEGDMA. Tensile bond strength of the resin increased with increasing the concentration of TEGDMA up to 6 wt% and, thereafter, decreased.
Mo 화학기상증착에 있어서 불순물 농도에 미치는 증착조건의 영향
배상석,서성교,홍은식,김승모,조미정,한혜정,이두성,김세훈,민석홍 국립7개대학공동논문집간행위원회 2001 공업기술연구 Vol.1 No.-
The thermal decomposition process of Mo(CO)_6 on chemical vapor deposition was investigated by analyzing the effects of deposition temperature, pressure, and reaction gas on the phase change of deposited films. Mo_2C was deposited at or below 350℃ due to the incompleteness of thermal decomposition of Mo(CO)_6, but Mo films was successfully deposited at 400℃ or higher temperatures. The variation of deposition pressure did not change the dependence of decomposition process on deposition temperature. The activation energy of surface reaction was 5.8 ㎉/mole.
Lee, Eun-Ju,Park, Kwan-Sik,Jeon, In-Sook,Cho, Jae-Woon,Lee, Sang-Jeon,Choy, Hyun E.,Song, Ki-Duk,Lee, Hak-Kyo,Choi, Joong-Kook Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.7
Lysosomes are cellular organelles containing diverse classes of catabolic enzymes that are implicated in diverse cellular processes including phagocytosis, autophagy, lipid transport, and aging. Lysosome-associated membrane proteins (LAMP-1 and LAMP-2) are major glycoproteins important for maintaining lysosomal integrity, pH, and catabolism. LAMP-1 and LAMP-2 are constitutively expressed in Salmonella-infected cells and are recruited to Salmonella-containing vacuoles (SCVs) as well as Salmonella- induced filaments (Sifs) that promote the survival and proliferation of the Salmonella. LAMP-3, also known as DC-LAMP/CD208, is a member of the LAMP family of proteins, but its role during Salmonella infection remains unclear. DNA microarray analysis identified LAMP-3 as one of the genes responding to LPS stimulation in THP-1 macrophage cells. Subsequent analyses reveal that LPS and Salmonella induced the expression of LAMP-3 at both the transcriptional and translational levels. Confocal Super resolution N-SIM imaging revealed that LAMP-3, like LAMP-2, shifts its localization from the cell surface to alongside Salmonella. Knockdown of LAMP-3 by specific siRNAs decreased the number of Salmonella recovered from the infected cells. Therefore, we conclude that LAMP-3 is induced by Salmonella infection and recruited to the Salmonella pathogen for intracellular proliferation.
Lee, Dong-Eun,Na, Jin Hee,Lee, Sangmin,Kang, Choong Mo,Kim, Hun Nyun,Han, Seung Jin,Kim, Hyunjoon,Choe, Yearn Seong,Jung, Kyung-Ho,Lee, Kyo Chul,Choi, Kuiwon,Kwon, Ick Chan,Jeong, Seo Young,Lee, Kyung American Chemical Society 2013 MOLECULAR PHARMACEUTICS Vol.10 No.6
<P>An efficient and straightforward method for radiolabeling nanoparticles is urgently needed to understand the <I>in vivo</I> biodistribution of nanoparticles. Herein, we investigated a facile and highly efficient strategy to prepare radiolabeled glycol chitosan nanoparticles with <SUP>64</SUP>Cu via a strain-promoted azide–alkyne cycloaddition strategy, which is often referred to as click chemistry. First, the azide (N<SUB>3</SUB>) group, which allows for the preparation of radiolabeled nanoparticles by copper-free click chemistry, was incorporated to glycol chitosan nanoparticles (CNPs). Second, the strained cyclooctyne derivative, dibenzyl cyclooctyne (DBCO) conjugated with a 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) chelator, was synthesized for preparing the preradiolabeled alkyne complex with <SUP>64</SUP>Cu radionuclide. Following incubation with the <SUP>64</SUP>Cu-radiolabeled DBCO complex (DBCO-PEG<SUB>4</SUB>-Lys-DOTA-<SUP>64</SUP>Cu with high specific activity, 18.5 GBq/μmol), the azide-functionalized CNPs were radiolabeled successfully with <SUP>64</SUP>Cu, with a high radiolabeling efficiency and a high radiolabeling yield (>98%). Importantly, the radiolabeling of CNPs by copper-free click chemistry was accomplished within 30 min, with great efficiency in aqueous conditions. In addition, we found that the <SUP>64</SUP>Cu-radiolabeled CNPs (<SUP>64</SUP>Cu-CNPs) did not show any significant effect on the physicochemical properties, such as size, zeta potential, or spherical morphology. After <SUP>64</SUP>Cu-CNPs were intravenously administered to tumor-bearing mice, the real-time, <I>in vivo</I> biodistribution and tumor-targeting ability of <SUP>64</SUP>Cu-CNPs were quantitatively evaluated by microPET images of tumor-bearing mice. These results demonstrate the benefit of copper-free click chemistry as a facile, preradiolabeling approach to conveniently radiolabel nanoparticles for evaluating the real-time <I>in vivo</I> biodistribution of nanoparticles.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/mpohbp/2013/mpohbp.2013.10.issue-6/mp300601r/production/images/medium/mp-2012-00601r_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/mp300601r'>ACS Electronic Supporting Info</A></P>