Two Unrecorded Apiospora Species Isolated from Marine Substrates in Korea with Eight New Combinations (A. piptatheri and A. rasikravindrae)

Kwon Sun Lul,Cho Minseo,Lee Young Min,Kim Changmu,Lee Soo Min,Ahn Byoung Jun,Lee Hanbyul,Kim Jae-Jin 한국균학회 2022 Mycobiology Vol.50 No.1

Although Apiospora Sacc. has previously been considered a sexual morph of Arthrinium spe- cies on the basis of phylogenetic, morphological, and ecological diagnoses, a recent study delimited these as different species. Recently, 14 species, including eight new species, of marine Arthrinium have been reported from Korea. Six known species have previously been renamed as species in the genus Apiospora (A. arundinis, A. marii, A. piptatheri, A. rasikravin- drae, A. sacchari, and A. saccharicola). However, the eight new species of marine Arthrinium (Ar. agari, Ar. arctoscopi, Ar. fermenti, Ar. koreanum, Ar. marinum, Ar. pusillispermum, Ar. sar- gassi, and Ar. taeanense) are yet to be studied, and thus the taxonomic status of these spe- cies remains to be clarified. In this study, we conducted phylogenetic analyses using the internal transcribed spacer, 28S large subunit ribosomal RNA gene, translation elongation factor 1-alpha, and beta-tubulin regions to confirm the phylogenetic position of these eight species. Based on these analyses, we re-identified the eight Arthrinium species as new com- binations in Apiospora. Additionally, among the six known Apiospora species, two (A. pipta- theri and A. rasikravindrae) have not previously been recorded in Korea. On the basis of morphological and molecular analyses, we report these as new species in Korea. Herein, we present scanning electron micrographs detailing the morphologies of these species, along with phylogenetic trees and detailed descriptions.

Identification of three wood decay fungi in Yeoninsan Provincial Park, Korea

Kwon, Sun Lul,Jang, Seokyoon,Kim, Min-Ji,Kim, Kyeongwon,Kim, Chul-Whan,Jang, Yeongseon,Lim, Young Woon,Kim, Changmu,Kim, Jae-Jin The National Institute of Biological Resources 2018 Journal of species research Vol.7 No.3

Though several wood decay fungi have been reported in the world-wide, only about 600 wood decay fungi have been reported in Korea to date. Thus, the objective of this study was to secure resources for the wood decay fungi in Korea. We investigated wood decay fungi in Yeoninsan Provincial Park, Korea, and the collected specimens were identified based on ITS sequence analysis. Two species were unrecorded species in Korea: Postia hirsuta (Polyporales, Basidiomycota) and Hyphodontia reticulata (Hymenochaetales, Basidiomycota). Another species was previously reported without detailed description: Ceriporia alachuana (Polyporales, Basidiomycota). Here, we provided additional detailed microscopic features and phylogenetic analysis of these species.

Note of Five Unrecorded Mushrooms Including Three Rare Species on Mount Juwang in Korea

( Sun Lul Kwon ),( Seokyoon Jang ),( Changmu Kim ),( Young Woon Lim ),( Jae-jin Kim ) 한국균학회 2020 Mycobiology Vol.48 No.3

The Mount Juwang was designated as a national park in 1976 because of its unique bedrock geology. Although Juwang National Park has maintained its natural ecosystem well, few macrofungal surveys have been carried out. As a part of a project supported by the National Institute of Biological Resources (NIBR) for the discovery of indigenous fungal species, we surveyed the mushrooms in Juwang National Park from 2018 to 2019. The macrofungi were identified based on morphological and molecular analyses. Among these fungi, five specimens were identified as species previously unrecorded in Korea: Calocybe decolorata, Crepidotus brunnescens, Mycena pearsoniana, Psathyrella phegophila, and P. sulcatotuberculosa. Three of these species are known to be rare in the world: Crepidotus brunnescens, P. phegophila, and P. sulcatotuberculosa. In this study, we provide detailed morphological descriptions of the five unrecorded species from Mt. Juwang in Korea.

Diversity of the Bambusicolous Fungus Apiospora in Korea: Discovery of New Apiospora Species

Sun Lul Kwon,Minseo Cho,Young Min Lee,Hanbyul Lee,Changmu Kim,Gyu-Hyeok Kim,Jae-Jin Kim 한국균학회 2022 Mycobiology Vol.50 No.5

Many Apiospora species have been isolated from bamboo plants – to date, 34 bambusico- lous Apiospora species have been recorded. They are known as saprophytes, endophytes, and plant pathogens. In this study, 242 bambusicolous Apiospora were isolated from various bamboo materials (branches, culms, leaves, roots, and shoots) and examined using DNA sequence similarity based on the internal transcribed spacer, 28S large subunit ribosomal RNA gene, translation elongation factor 1-alpha, and beta-tubulin regions. Nine Apiospora species (Ap. arundinis, Ap. camelliae-sinensis, Ap. hysterina, Ap. lageniformis sp. nov., Ap. para- phaeosperma, Ap. pseudohyphopodii sp. nov., Ap. rasikravindrae, Ap. saccharicola, and Ap. sar- gassi) were identified via molecular analysis. Moreover, the highest diversity of Apiospora was found in culms, and the most abundant species was Ap. arundinis. Among the nine Apiospora species, two (Ap. hysterina and Ap. paraphaeosperma) were unrecorded in Korea, and the other two species (Ap. lageniformis sp. nov. and Ap. pseudohyphopodii sp. nov.) were potentially novel species. Here, we describe the diversity of bambusicolous Apiospora species in bamboo organs, construct a multi-locus phylogenetic tree, and delineate morphological features of new bambusicolous Apiospora in Korea.

국내 대나무 곰팡이 신종 및 미기록종 연구

권선률 ( Sun Lul Kwon ),조민서 ( Minseo Cho ),유연재 ( Yeonjae Yoo ),이상현 ( Sanghyun Lee ),권대영 ( Dae Young Kwon ),김재진 ( Jae-jin Kim ) 한국목재공학회 2024 한국목재공학회 학술발표논문집 Vol.2024 No.1

Notes of 15 unrecorded macrofungi in Korea

Minseo Cho,Sun Lul Kwon,장석윤,유연재,Sang-Hyun Lee,Dae Young Kwon,Changmu Kim,임영운,김재진 국립생물자원관 2024 Journal of species research Vol.13 No.1

Macrofungi are essential decomposers in the forest environment. Although more than 70% of the land is mountainous, there has been a lack of research on mushroom diversity in Korea compared to the global species estimation. For this reason, the need for further research became apparent. The surveys were conducted from 2014 to 2022 nationwide. As a result, 15 unrecorded macrofungal species were discovered: Agaricus thiersii, Baorangia alexandri, Boletellus putuoensis, Entoloma bulakhae, Entoloma pygmaeopapillatum, Entoloma subtenuicystidiatum, Gerronema kuruvense, Hyphoderma nudicephalum, Hyphoderma tenue, Macrolepiota subcitrophylla, Mycena jingyinga, Mycena yuezhuoi, Ophiocordyceps vespulae, Scytinostroma acystidiatum, and Steccherinum straminellum. These species are identified based on morphological, molecular, and phylogenetic analyses using the internal transcribed spacer (ITS) region and the nuclear large subunit rRNA (LSU) region. Here, we provided macro- and micro-morphological figures with phylogenetic trees to support 15 species as unrecorded to Korea.

New Report of Three Unrecorded Species in Trichoderma harzianum Species Complex in Korea

( Seokyoon Jang ),( Sun Lul Kwon ),( Hanbyul Lee ),( Yeongseon Jang ),( Myung Soo Park ),( Young Woon Lim ),( Changmu Kim ),( Jae-jin Kim ) 한국균학회 2018 Mycobiology Vol.46 No.3

The genus Trichoderma (Hypocreaceae, Ascomycota) consists of globally distributed fungi. Among them, T. harzianum, one of the most commonly collected Trichoderma species, had been known as a polyphyletic or aggregate species. However, a total of 19 species were determined from the polyphyletic groups of T. harzianum. Thus, we explored Korean “T. harzianum” specimens that were collected in 2013-2014. These specimens were re-examined based on a recent study with translate elongation factor 1-alpha (EF1a) sequences to reveal cryptic Trichoderma species in Korea. As a result, four different species, T. afroharzianum, T. atrobruneum, T. pyramidale, and T. harzianum, were identified. Except T. harzianum, the other three species have not been reported in Korea. In this work, we describe these species and provide figures.

계면활성제 생성능과 세포 표면 소수성을 가진 세균 균주들에 의한 석유탄화수소의 생분해

권선률,송홍규,Kwon, Sun-Lul,Song, Hong-Gyu 한국미생물학회 2017 미생물학회지 Vol.53 No.4

다른 탄화수소 분해 방법을 가진 여러 세균을 유류-오염 토양에서 분리하였다. Acinetobacter calcoaceticus SL1은 기름-분산 시험에서 계면활성제-생성능을 보였으며 경유와 n-hexane에 대해 각각 43.6과 54.5%의 유화활성을 나타내었다. 또한 높은 세포 표면 소수성을 가졌는데 이는 탄화수소에 쉽게 부착하여 그것을 분해할 수 있게 한다. 이 균주는 1,000 mg/L의 n-octadecane과 naphthalene을 3일 내에 100% 분해하였으며, 1,000 mg/L의 경유는 7일 동안 72.3%, 그리고 10,000 mg/L로 오염시킨 토양에서는 4주 간 78%를 제거하였다. 다른 분리균주 Bacillus amyloliquefaciens S10과 B. subtilis GO9는 생물계면활성제를 생산할 수 있으며 기름-분산 시험에서 각각 6.34와 2.5 cm직경의 투명대를 형성하였다. 배양 중 그들의 배양 상등액은 표면장력이 74.6 mN/m으로부터 각각 34.4와 33.3 mN/m으로 감소하였으며, 배양 상등액의 critical micelle concentration은 각각 2.0과 5.9%이었다. A. calcoaceticus SL1과 B. amyloliquefaciens S10의 컨소시엄은 10,000 mg/L의 경유를 3일만에 77.8% 분해하였는데 이는 A. calcoaceticus SL1 단독에 의한 것보다 높은 분해능이었다. 만일 이 세균들을 유류-오염 부지에 컨소시엄으로 같이 처리한다면 높은 석유탄화수소 제거율을 나타낼 수 있을 것이다. Some bacteria with different mechanisms for hydrocarbon degradation were isolated from oil-contaminated soils in Korea. Isolate Acinetobacter calcoaceticus SL1 showed biosurfactant- producing activity in oil-spreading test, and it exhibited a good emulsifying activity of 43.6 and 54.5% for diesel oil and n-hexane, respectively. It also has high cell surface hydrophobicity which can make it easily attaches to hydrocarbons and degrade them. It degraded 100% of 1,000 mg/L of n-octadecane and naphthalene, respectively in 3 days, 72.3% of 1,000 mg/L diesel oil in 7 days and 78.0% of 10,000 mg/L diesel oil in oil-contaminated soil during 28 days. Isolated strains Bacillus amyloliquefaciens S10 and B. subtilis GO9 can produce biosurfactant and formed 6.34 and 2.5 cm diameter of clear zones, respectively in oil-spreading test. Surface tension of their culture supernatant reduced from 74.6 to 34.4 and 33.3 mN/m, respectively during incubation, and critical micelle concentrations of culture supernatants were 2.0 and 5.9%, respectively. Consortium of A. calcoaceticus SL1 and B. amyloliquefaciens S10 degraded 77.8% of 10,000 mg/L diesel oil in 3 days, which indicated more efficient oil degradation than that by A. calcoaceticus SL1 alone. If these bacteria were applied together as a consortium to oil-contaminated sites, they may show a high removal rate of petroleum hydrocarbons.

Seven Unrecorded Indigenous Fungi from Mudeungsan National Park in Korea

Cho Minseo,Kwon Sun Lul,Heo Young Mok,Lee Young Min,Lee Hanbyul,Kim Changmu,Ahn Byoung Jun,Kim Jae-Jin 한국균학회 2022 Mycobiology Vol.50 No.4

Fungi act as important decomposers in the forest environment. They recycle essential nutrients, promote plant growth through mycorrhizal relationships, and act as food for small animals. Samples of 265 indigenous fungal species were collected from Mudeungsan National Park in 2020. These species were identified based on morphological, molecular, and phylogenetic analyses using the internal transcribed spacer (ITS), nuclear large subunit rRNA (LSU), and RNA polymerase II second largest subunit (rpb2) regions. Subsequently, seven species were identified as unrecorded species in Korea: Cordyceps cicadae, Dentocorticium bicolor, Hymenochaete nanospora, Physisporinus crataegi, Rigidoporus piceicola, Russula raoultii, and Scutellinia crinita. This study reveals their detailed macro- and microscopic morphological characteristics with phylogenetic trees to report them as unrecorded species in Korea.

Investigation of Filamentous Fungi Producing Safe, Functional Water-Soluble Pigments

( Young Mok Heo ),( Kyeongwon Kim ),( Sun Lul Kwon ),( Joorim Na ),( Hanbyul Lee ),( Seokyoon Jang ),( Chul Hwan Kim ),( Jinho Jung ),( Jae-jin Kim ) 한국균학회 2018 Mycobiology Vol.46 No.3

The production of water-soluble pigments by fungal strains indigenous to South Korea was investigated to find those that are highly productive in submerged culture. Among 113 candidates, 34 strains that colored the inoculated potato dextrose agar medium were selected. They were cultured in potato dextrose broth and extracted with ethanol. The productivity, functionality (radical-scavenging activities), and color information (CIELAB values) of the pigment extracts were measured. Five species produced intense yellowish pigments, and two produced intense reddish pigments that ranked the highest in terms of absorbance units produced per day. The pigment extracts of Penicillium miczynskii, Sanghuangporus baumii, Trichoderma sp. 1, and Trichoderma afroharzianum exhibited high radical-scavenging activity. However, the S. baumii extract showed moderate toxicity in the acute toxicity test, which limits the industrial application of this pigment. In conclusion, P. miczynskii KUC1721, Trichoderma sp. 1 KUC1716, and T. afroharzianum KUC21213 were the best fungal candidates to be industrial producers of safe, functional water-soluble pigments.