Investigation on the Effect of Asymmetric Cyclic Loading on Ratcheting Deformation and Bulk Texture Development in HSLA Steel

Pushpendra Kumar Dwivedi,R. Vinjamuri,S. K. Sahoo,Krishna Dutta 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.8

Ratcheting deformation in association with bulk texture evolution in HSLA steel is studied in this investigation. The cyclicdeformation behaviors and damage modes of the specimens are obtained at room temperature, employing asymmetric stresscontrolledtests under various combinations of mean stress and stress amplitudes. Bulk texture measurements of the specimensare done adopting an X-ray diffraction analysis. The results exhibit an increase in strain accumulation from 0.23 to 30.61%associated with a sharp reduction in ratcheting life from 3320 to 880 cycles by the increment in mean stress/stress amplitude. A continuous cyclic softening until the final failure is observed for all the ratcheting tests. It is noticed that variation of meanstress is more detrimental than stress amplitude in controlling strain accumulation while the effect of stress amplitude is morepronounced than mean stress for the reduction of ratcheting life. The fracture surface morphology indicates typical striationformation in the crack propagation region along with overload failure. The bulk texture studies indicate that there is a transitionbetween ‘ϒ’ fiber and ‘ζ’ fiber during all loading conditions. The texture intensity is increased from 5.71 to 11.68 withprogressive ratcheting strain accumulation. Here also, influence of stress amplitude is more significant than the mean stress.

Molecular Characterization of Rathi and Tharparkar Indigenous Cattle (Bos indicus) Breeds by RAPD-PCR

Sharma, Amit Kumar,Bhushan, Bharat,Kumar, Sanjeev,Kumar, Pushpendra,Sharma, Arjava,Kumar, Satish Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.9

Random amplification of polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) analysis was carried out using DNA samples of 30 animals of Rathi cattle and 42 animals of Tharparkar cattle. Genomic DNA was isolated as per standard protocol and evaluated for its quality, purity and concentration. Twenty three random primers were screened out of which 15 primers yielded satisfactory amplifications and were used for further analysis. Average numbers of polymorphic fragments per primer were 7.07${\pm}$0.86 in Rathi and 6.80${\pm}$0.61 in Tharparkar cattle. The percentage of polymorphic bands in these two cattle breeds were 86 and 87%, respectively. Within breed genetic similarities for pooled over primers in the animals of Rathi and Tharparkar breeds were .577${\pm}$0.30 and 0.531${\pm}$0.02, respectively on the basis of band frequency (BF) and 0.645${\pm}$0.04 and 0.534${\pm}$0.04, respectively on the basis of band sharing (BS). Averages of between breed genetic similarities for pooled over primers were 0.97 and 0.92 according to BF and BS, respectively, which reflect higher degree of genetic similarity between Rathi and Tharparkar cattle breeds. Index of genetic distance based on BF and BS for pooled over primers was 0.030${\pm}$0.011 and 0.088${\pm}$0.031, respectively. Percentage of polymorphic bands and within-breed genetic similarities on the basis of band frequency (BF) and band sharing (BS) for pooled over primers revealed higher genetic similarity in Rathi than Tharparkar cattle population. High estimates of between breed genetic similarities for pooled over primers indicated that either Rathi is having decent from Tharparkar or both the cattle breeds are having common descent. Low value of Index of genetic distances between these two cattle breeds may be due to the fact that Rathi and Tharparkar cattle breeds are the native of Thar Desert in Northwest India. The results of between breed genetic distances also confirm the existence of high degree of genetic similarity between these two breeds of cattle.

Comparative analysis of VMT genes/proteins in selected plant species with emphasis on bread wheat (Triticum aestivum L.)

Sharma Hemant,Shayaba,Kumar Rahul,Kumar Jitendra,Bhadana Deepa,Batra Ritu,Singh Rakhi,Kumar Sachin,Roy Joy K,Balyan Harindra S.,Gupta Pushpendra K. 한국유전학회 2023 Genes & Genomics Vol.45 No.11

Background In recent years, the study of molecular basis of uptake, transport and utilization of grain Fe/Zn (GFe/GZn) in wheat has been an active area of research. As a result, it has been shown that a number of transporters are involved in uptake and transport of Fe. In a recent study, knockout of a transporter gene OsVMT (VACUOLAR MUGINEIC ACID TRANSPORTER) in rice was shown to be involved in Fe homoeostasis. Objective In this study, we analysed VMT genes among six monocots and three dicots with major emphasis on wheat VMT genes (TaVMTs), taking OsVMT gene as a reference. Methods and results Using OsVMT gene as a reference, VMT genes were identified and sequence similarities were examined among six monocots and three dicots. Each VMT protein carried one functional domain and 7 to 10 distinct motifs (including 9 novel motifs). The qRT-PCR analysis showed differential expression by all the six TaVMT genes in pairs of contrasting wheat genotypes with high (FAR4 and WB02) and low (K8027 and HD3226) GFe/GZn at two different grain filling stages (14 DAA and 28 DAA). TaVMT1 genes showed up-regulation in high GFe/Zn genotypes relative to low GFe/Zn genotypes, whereas the TaVMT2 genes showed down-regulation or nonsignificant up-regulation in a few cases. Conclusions At 14 DAA, each of the six TaVMT genes exhibited higher expression in wheat genotypes with high GFe and GZn relative to those with low GFe and GZn, suggesting major role of VMT genes in improvement of grain Fe/Zn homoeostasis, thus making TaVMT genes useful for improvement in Fe/Zn in wheat grains.

Effects of carbamate pesticides intermediates on Escherichia coli membrane architecture: An in vitro and in silico approach

Pushpendra Singh,Manish Kumar Tripathi,Mohammad Yasir,Ashish Ranjan,Rahul Shrivastava 환경독성보건학회 2021 환경독성보건학회지 Vol.36 No.3

Methyl isocyanate (MIC), a low molecular weight synthetic aliphatic compound, having an isocyanate group (−NCO), has industrial application. In this study, the effects of methyl isocyanate and its mechanism on outer membrane protein of Escherichia coli were observed using experimental and computational methods. In vitro exposure of N-succinimidyl N-methylcarbamate (NSNM) a synthetic analogue of MIC on E. coli to a final concentration of 2 mM was found to affect the growth curve pattern and changes in cell morphology. Molecular docking studies of MIC and NSNM with E. coli outer membrane protein (OmpW, OmpX, OmpF OmpA), and periplasmic domain (PAL) were performed. The in-silico results revealed that outer membrane protein OmpF showed the highest negative binding energy, i.e. ΔG -4.11 kcal/mole and ΔG -3.19 kcal/mole by NSNM and MIC as compared to other proteins. Our study concludes that methyl isocyanate retains lethal toxicity which leads to cell death due to the membrane protein damage of E. coli membrane.

Interplay of organizational identification, regulatory focused job crafting and job satisfaction in management of emerging job demands: evidence from public sector enterprises

Sanjeet Kumar Sameer,Pushpendra Priyadarshi 한국행정학회 2021 International Review of Public Administration Vol.26 No.1

Public sector enterprises (PSEs) face market complexities like any other commercial organization. To ensure sustenance of such enterprises, effective management of emerging job demands by their employees is crucial. Acknowledging relevance of this issue, the present study examines the role of employees’ organizational identification, as a job resource, in promotion- and preventionfocused job crafting and its effect on job satisfaction, by applying job demands-resources and regulatory focus theories. Data collected through survey questionnaire from 433 executives of public sector energy companies of India were analyzed to test hypotheses. The study found a positive effect of organizational identification on promotion- and prevention-focused job crafting and job satisfaction. Promotion-focused job crafting partially mediated the relationship of organizational identification and job satisfaction unlike prevention-focused job crafting. These unique findings may be useful from the perspective of effective job demands-resources management in a dynamic and complex operating environment of PSEs. Theoretical contributions and practical implications have been discussed.

Effect of Leptin and IGFBP-3 Gene Polymorphisms on Serum IgG Level of Cattle Calves

Choudhary, Vivek,Kumar, Pushpendra,Saxena, V.K.,Bhattacharya, T.K.,Bhushan, Bharat,Sharma, Arjava,Ahmed, K.A. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.8

Leptin and IGFBP-3 are two proteins that play an important role in growth and metabolism of the animals. They are also involved in the immune function of animals and, thus, are candidate genes for the study of association with immune functions. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) of these two genes was done to screen 64 crossbred (Holstein Friesian${\times}$Hariana) female calves of one year of age. From each RFLPs (fragments) three genotypes were observed. In all the RFLPs the mutant homozygotes were very less in numbers and, hence, were excluded from the least squares analysis. The serum IgG level was estimated using SRID assay. The mean level of serum IgG was $28.83{\pm}2.73mg/ml$. The effect of these identified genotypes on serum IgG level of calves at one year of age was analysed using least squares analysis. The HaeIII RFLP-AB genotype had significantly (p<0.05) higher serum IgG level ($31.86{\pm}3.05$) than the HaeIII RFLP-AA ($25.62{\pm}2.96$) genotype. There was no significant effect of leptin genotypes on the IgG level. The present results indicated a role of the IGFBP-3 gene on serum IgG level of cattle calves.

Nucleotide Sequencing and PCR-RFLP of Insulin-like Growth Factor Binding Protein-3 Gene in Riverine Buffalo (Bubalus bubalis)

Padma, B.,Kumar, Pushpendra,Choudhary, V.,Dhara, S.K.,Mishra, A.,Bhattacharya, T.K.,Bhushan, B.,Sharma, Arjava Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.7

Insulin-like growth factor binding protein-3 (IGFBP-3) gene is a structural gene associated with the growth and development of the animals. The present investigation was carried out to unravel nucleotide sequence and polymerase chain reactionrestriction fragment polymorphism (PCR-RFLP) of IGFBP-3 gene in buffalo. Genomic DNA was isolated from a total of 157 animals belonging to Murrah, Surti, Jaffarabadi and Nagpuri breeds of Indian riverine buffalo. A 655 bp of IGFBP-3 gene was amplified in all the breeds and amplicons were digested with Hae III, Taq I and Msp I restriction enzymes. On digestion with Hae III yielded single restriction pattern of 8 fragments of sizes 201, 165, 154, 56, 36, 19, 16 and 8 bp in all the animals studied. Similarly Taq I and Msp I also revealed single restriction pattern yielding fragments of sizes 240 and 415 bp and 145 and 510 bp, respectively. This shows nonpolymorphic nature of restriction sites in buffalo. Nucleotide sequencing of 587 bp of IGFBP-3 gene in Murrah buffalo was done and submitted to the GenBank (Accession No. AY304829). Nucleotide sequencing revealed an addition of 4 bases in the intronic region as compared to cattle.

How emotionally intelligent employees manage their internal employability through role-based job crafting? – evidence from public sector enterprises

Sameer Sanjeet Kumar,Priyadarshi Pushpendra 한국행정학회 2023 International Review of Public Administration Vol.28 No.3

Acknowledging the relevance of work environment in influencing job-related choices, this paper examines the relationship between emotional intelligence, role-based job crafting i.e. task, relational and cognitive crafting, job autonomy and internal employability. The role of job autonmy as a moderator and role-based job crafting as mediator between emotional intelligence and internal employability has also been examined. The study was conducted on 296 managers working in Indian public sector enterprises and found interesting findings including a positive and direct effect of emotional intelligence on all three forms of role-based job crafting and internal employability. The results for mediation effect of job crafting and moderation effect of job autonomy varied depending upon the form of role-based job crafting. The paper discusses theoretical implications and practical relevance of these findings and may provide a direction for interlinking job demands-resources and conservation of resource theories.

PCR-SSCP of Serum Lysozyme Gene (Exon-III) in Riverine Buffalo and Its Association with Lysozyme Activity and Somatic Cell Count

Sahoo, Nihar Ranjan,Kumar, Pushpendra,Bhushan, Bharat,Bhattacharya, T.K.,Sharma, Arjava,Dayal, Sanker,Pankaj, Prabhat Kumar,Sahoo, Monalisa Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.8

Serum lysozyme gene is one of the important genes influencing the immune system as its product can cause lysis of bacterial cell wall by cleaving the peptidoglycan layer. The present investigation on the serum lysozyme gene of Indian riverine buffalo was undertaken with the objectives to identify and characterize single nucleotide polymorphic patterns by PCR-SSCP method as well as to study the effect of different genotypes on serum lysozyme activity and somatic cell count. A total of 280 animals comprising four different famous bubaline breeds (Murrah, Mehsana, Surti and Bhadawari), spread over six different farms across the country were used for this study. A 276 bp (partial intron 2, complete exon 3 and partial intron 3) fragment of lysozyme gene was screened for polymorphism using the SSCP technique. Four genotypes namely AA, AB, BC and AC were observed, out of which BC genotype was found to be the most frequent. Among these three alleles, C allele (0.38) was most prevalent in these populations. Various SSCP allelic variants were cloned for sequencing and sequences were submitted to NCBI Genbank. From the alignment of the nucleotide sequences of various allelic variants, it was found that there were differences in 12 positions among the alleles, out of which maximum variation (at 8 places) was found in the intronic region. The allele A was closer to allele-C than allele-B. Allele B was phylogenetically equidistant from both of the other alleles. Mean lysozyme activity determined in serum samples of different animals of Murrah buffalo was $27.35{\pm}2.42\;{\mu}g$ per ml of serum, whereas the mean somatic cell count was $1.25{\pm}0.13{\times}10^5$ cells per ml of milk. The SSCP pattern-wise effects of various genotypes on lysozyme activity and SCC were analyzed. Although the mean values were apparently different in various genotypes, these differences were statistically non-significant. It can be concluded that the riverine buffaloes are sufficiently polymorphic with respect to serum lysozyme gene. The absence of AA genotype in Bhadawari breed of buffalo can be considered as a marker for breed characterization. The difference of four nucleotides in exon-3 indicates high selection pressure on the gene.

Characterization of MHC DRB3.2 Alleles of Crossbred Cattle by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism

Paswan, Chandan,Bhushan, Bharat,Patra, B.N.,Kumar, Pushpendra,Sharma, Arjava,Dandapat, S.,Tomar, A.K.S.,Dutt, Triveni Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.9

The present investigation was undertaken to study the genetic polymorphism of the DRB3 exon 2 in 75 crossbred cattle by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Five genotypes i.e. HaeIII-a, HaeIII-b, HaeIII-e, HaeIII-ab and HaeIII-ae were observed when the 284 bp PCR products were digested with HaeIII restriction enzyme. The corresponding frequencies of these patterns were 0.53, 0.04, 0.01, 0.38 and 0.04, respectively. Digestion with RsaI restriction enzyme resolved 24 different restriction patterns. The frequencies of these patterns ranged from 0.013 (RsaI-f, RsaI-k and RsaI-c/n) to 0.120 (RsaI-n). The results revealed that the crossbred cows belonged to the RsaI patterns namely b, k, l, a/l, d/s, l/n, l/o and m/n, whose corresponding frequencies were 0.027, 0.013, 0.040, 0.027, 0.040, 0.067, 0.027 and 0.067, respectively. Digestion of the 284 bp PCR product of DRB3.2 gene with PstI in the crossbred cattle did not reveal any restriction site. These results suggested the absence of the recognition site in some of the animals. These results also revealed that the crossbred cows studied were in homozygous as well as heterozygous condition. On the basis of the above results it can be concluded that the DRB3.2 gene was found to be highly polymorphic in the crossbred cattle population.