A Genetic Analysis of Taoyuan Pig and Its Phylogenetic Relationship to Eurasian Pig Breeds

Kuan-Yi Li,Kuang-Ti Li,Chun-Chun Cheng,Chia-Hsuan Chen,Chien-Yi Hung,Yu-Ten Ju 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.4

Taoyuan pig is a native Taiwan breed. According to the historical record, the breed was first introduced to Taiwan from Guangdong province, Southern China, around 1877. The breed played an important role in Taiwan’s early swine industry. It was classified as an indigenous breed in 1986. After 1987, a conserved population of Taoyuan pig was collected and reared in isolation. In this study, mitochondrial DNA sequences and 18 microsatellite markers were used to investigate maternal lineage and genetic diversity within the Taoyuan pig population. Population differentiation among Taoyuan, Asian type, and European type pig breeds was also evaluated using differentiation indices. Only one D-loop haplotype of the Taoyuan pig was found. It clustered with Lower Changjiang River Basin and Central China Type pig breeds. Based on the polymorphism of microsatellite markers, a positive fixation index value (FIS) indicates that the conserved Taoyuan population suffers from inbreeding. In addition, high FST values (>0.2105) were obtained, revealing high differentiation among these breeds. Non-metric multi-dimensional scaling showed a clear geometric structure among 7 breeds. Together these results indicate that maternally Taoyuan pig originated in the Lower Changjiang River Basin and Central China; however, since being introduced to Taiwan differentiation has occurred. In addition, Taoyuan pig has lost genetic diversity in both its mitochondrial and nuclear genomes.

Ginseng essence, a medicinal and edible herbal formulation, ameliorates carbon tetrachloride-induced oxidative stress and liver injury in rats

Kuan-Hung Lu,Ching-Yi Weng,Wei-Cheng Chen,Lee-Yan Sheen 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.3

Background: Ginseng essence (GE) is a formulation comprising four medicinal and edible herbs including ginseng (Panax ginseng), American ginseng (Panax quinquefolius), lotus seed (Nelumbo nucifera), and lily bulb (Lilium longiflorum). This study was aimed at investigating the hepatoprotective effect of GE against carbon tetrachloride (CCl4)-induced liver injury in rats. Methods: We treated Wistar rats daily with low, medium, and high [0.625 g/kg body weight (bw), 1.25 g/ kg bw, and 3.125 g/kg bw, respectively] doses of GE for 9 wk. After the 1st wk of treatment, rats were administered 20% CCl4 (1.5 mL/kg bw) two times a week to induce liver damage until the treatment ended. Results: Serum biochemical analysis indicated that GE ameliorated the elevation of aspartate aminotransferase and alanine aminotransferase and albumin decline in CCl4-treated rats. Moreover, CCl4- induced accumulation of hepatic total cholesterol and triglyceride was inhibited. The hepatoprotective effects of GE involved enhancing the hepatic antioxidant defense system including glutathione, glutathione peroxidase, glutathione reductase, glutathione S-transferase, superoxide dismutase, and catalase. In addition, histological analysis using hematoxylin and eosin and Masson’s trichrome staining showed that GE inhibited CCl4-induced hepatic inflammation and fibrosis. Furthermore, immunohistochemical staining of alpha-smooth muscle actin indicated that CCl4-triggered activation of hepatic stellate cells was reduced. Conclusion: These findings demonstrate that GE improves CCl4-induced liver inflammation and fibrosis by attenuating oxidative stress. Therefore, GE could be a promising hepatoprotective herbal formulation for future development of phytotherapy.

Validation of the Chinese Version of the Schizophrenia Cognition Rating Scale

Kuan-Wei Huang,Pao-Yen Lin,Yu Lee,Yu-Chi Huang,Chi-Fa Hung,Sheng-Yu Lee,Chih-Ken Chen,Liang-Jen Wang 대한신경정신의학회 2022 PSYCHIATRY INVESTIGATION Vol.19 No.7

Objective The Schizophrenia Cognition Rating Scale (SCoRS) is an interview-based assessment tool for evaluating the cognitive deficit and daily functioning of patients with schizophrenia.Methods Sixty-eight patients with schizophrenia and 68 age- and sex-matched healthy individuals were recruited to validate the Chinese version of SCoRS in this study. All participants underwent cognitive assessment using the SCoRS, which was verified by the Brief Assessment of Cognition in Schizophrenia (BACS), and the UCSD Performance-based Skills Assessment, Brief Version (UPSA-B). Patients with schizophrenia were additionally assessed using the Positive and Negative Syndrome Scale (PANSS).Results SCoRS ratings reported by patients (SCoRS-S), those reported by the interviewer (SCoRS-I), and SCoRS global scores (SCoRS-G) showed significant correlation with all subscales of the BACS and the UPSA-B. On receiver operating characteristic curve analysis, SCoRS-S, SCoRS-I, and SCoRS-G significantly differentiated patients with schizophrenia from healthy controls. Moreover, SCoRS-S and SCoRS-I ratings showed positive correlation with the negative symptoms and general symptoms of PANSS.Conclusion The Chinese version of SCoRS showed good discriminant, concurrent, and external validity, suggesting that it is a useful and convenient tool for assessment of cognitive function among Mandarin-speaking patients with schizophrenia in clinical practice.

Design of Three Phase Wye-Delta LLC Converter with Time Domain Analysis

Kuan-Hung Chen,Jing-Yuan Lin,Yi-Feng Lin,Yu-Chen Chang,Huang-Jen Chiu 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

In the paper, the performance of a 5 kW DC/DC Wye-Delta-based series resonant converter designed for 400V input voltage and 48V output voltage server power supply is evaluated. The paper also performs operation modes analysis, circuit characteristics analysis, and use time domain analysis on Wye-Delta-based series resonant converter for circuit design to replace FHA analysis due to waveform distortion. Finally, a full-load efficiency up to 96.07% and power-density up to 2.84W/cm3 server power supply is implemented.

A Genetic Analysis of Taoyuan Pig and Its Phylogenetic Relationship to Eurasian Pig Breeds

Li, Kuan-Yi,Li, Kuang-Ti,Cheng, Chun-Chun,Chen, Chia-Hsuan,Hung, Chien-Yi,Ju, Yu-Ten Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.4

Taoyuan pig is a native Taiwan breed. According to the historical record, the breed was first introduced to Taiwan from Guangdong province, Southern China, around 1877. The breed played an important role in Taiwan's early swine industry. It was classified as an indigenous breed in 1986. After 1987, a conserved population of Taoyuan pig was collected and reared in isolation. In this study, mitochondrial DNA sequences and 18 microsatellite markers were used to investigate maternal lineage and genetic diversity within the Taoyuan pig population. Population differentiation among Taoyuan, Asian type, and European type pig breeds was also evaluated using differentiation indices. Only one D-loop haplotype of the Taoyuan pig was found. It clustered with Lower Changjiang River Basin and Central China Type pig breeds. Based on the polymorphism of microsatellite markers, a positive fixation index value ($F_{IS}$) indicates that the conserved Taoyuan population suffers from inbreeding. In addition, high $F_{ST}$ values (>0.2105) were obtained, revealing high differentiation among these breeds. Non-metric multi-dimensional scaling showed a clear geometric structure among 7 breeds. Together these results indicate that maternally Taoyuan pig originated in the Lower Changjiang River Basin and Central China; however, since being introduced to Taiwan differentiation has occurred. In addition, Taoyuan pig has lost genetic diversity in both its mitochondrial and nuclear genomes.

Exploring The Importance of Knowledge of User manager in Hazardous Chemicals

Yun-Chen Liu,Chien-Jou Tsou,Ting-Wei Chang,Kuan-Hung Lin,Mei-Shih Kao 한국간호과학회 2019 한국간호과학회 학술대회 Vol.2019 No.10

Waterlogging influences the physiology index and antioxidant enzyme activity in Cucurbita maxima and Cucurbita moschata

Lin Kuan-Hung,Chen Shi-Peng,Su Yi-Ru,Tsai Yi-Heng,Lin Hsin-Hung 한국원예학회 2024 Horticulture, Environment, and Biotechnology Vol.65 No.1

Waterlogging is one of the major environmental challenges that can adversely impact squash growth, development, and productivity. Nevertheless, studies on the physiological changes of squash to waterlogging stress are scarce. Previously, we reported that the Cucurbita moschata (Cmo) Early Price (Ep) cultivar showed higher chlorophyll content (CC) and spectral refl ectance compared to the Cucurbita maxima (Cma) OK-101 (Ok) cultivar after waterlogging. In the present work, we further examined more physiological parameters in eight cultivars of Cmo and Cma, and studied whether these non-destruc tive indices could effi ciently identify various waterlogging-tolerant genotypes and were consistent with visual evaluations. Moreover, we investigated the eff ects of waterlogging on the antioxidant system of cucurbit species, and evaluated dynamic changes occurring in these plants during varying waterlog periods by determining physiological characteristics. The activ ity of ascorbate peroxidase (APX) and superoxide dismutase (SOD) showed a signifi cant increase in Ep plant leaves and roots after 72 h and 6 h of waterlogging, respectively, compared to Ok plants. In addition, Ep exhibited a higher tolerance to waterlogging than Ok in terms of enhanced root length, values in CC, spectral refl ectance, and APX and SOD activity, plus decreased levels of electrolyte leakage, 2,2-diphenyl-1-picrylhydrazyl free radical scavenging, and hydrogen peroxide levels. This study provides a comprehensive understanding of the physiological variations in response to waterlogging stress and can potentially be used to breed cucurbit species with waterlogging tolerance for a wetter future climate.

Ginseng essence, a medicinal and edible herbal formulation, ameliorates carbon tetrachloride-induced oxidative stress and liver injury in rats

Lu, Kuan-Hung,Weng, Ching-Yi,Chen, Wei-Cheng,Sheen, Lee-Yan The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.3

Background: Ginseng essence (GE) is a formulation comprising four medicinal and edible herbs including ginseng (Panax ginseng), American ginseng (Panax quinquefolius), lotus seed (Nelumbo nucifera), and lily bulb (Lilium longiflorum). This study was aimed at investigating the hepatoprotective effect of GE against carbon tetrachloride ($CCl_4$)-induced liver injury in rats. Methods: We treated Wistar rats daily with low, medium, and high [0.625 g/kg body weight (bw), 1.25 g/kg bw, and 3.125 g/kg bw, respectively] doses of GE for 9 wk. After the 1st wk of treatment, rats were administered 20% $CCl_4$ (1.5 mL/kg bw) two times a week to induce liver damage until the treatment ended. Results: Serum biochemical analysis indicated that GE ameliorated the elevation of aspartate aminotransferase and alanine aminotransferase and albumin decline in $CCl_4$-treated rats. Moreover, $CCl_4$-induced accumulation of hepatic total cholesterol and triglyceride was inhibited. The hepatoprotective effects of GE involved enhancing the hepatic antioxidant defense system including glutathione, glutathione peroxidase, glutathione reductase, glutathione S-transferase, superoxide dismutase, and catalase. In addition, histological analysis using hematoxylin and eosin and Masson's trichrome staining showed that GE inhibited $CCl_4$-induced hepatic inflammation and fibrosis. Furthermore, immunohistochemical staining of alpha-smooth muscle actin indicated that $CCl_4$-triggered activation of hepatic stellate cells was reduced. Conclusion: These findings demonstrate that GE improves $CCl_4$-induced liver inflammation and fibrosis by attenuating oxidative stress. Therefore, GE could be a promising hepatoprotective herbal formulation for future development of phytotherapy.

Application of Multiobjective Genetic Algorithms for Optimizing Aircraft Crew Pairing Problems

Tung-Kuan Liu,Chiu-Hung Chen,Jyh-Horng Chou,Shinn-Horng Chen,Ta-Yuan Chou 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8

This paper develops a method of inequalities-based multiobjective genetic algorithm (MMGA) to solve the crew pairing problems which mainly assign the crew members to each flight segment of a given time period, while complying with a variety of work regulations and collective agreements.There are various objectives to be addressed here and formulated into mathematical equations including legal turn-around time, dead-head, layover, flying time and flying duty period. Through the verification of the real-world study case, the proposed method can solve the crew pairing problems, and obtain feasible solutions which are more useful for practical use.

Auto-Scaling Model for Cloud Computing System

Che-Lun Hung,Yu-Chen Hu,Kuan-Ching Li 보안공학연구지원센터 2012 International Journal of Hybrid Information Techno Vol.5 No.2