Weed Occurrence at Several Arboreta of Korea and their Vegetation Analysis

Kyung Ku Shim,Yong Woong Kwon,Ho Kyung Song,Seok Bo Song,Jae Bok Hwang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Transglutaminase 2 inhibition found to induce p53 mediated apoptosis in renal cell carcinoma

Ku, Bo Mi,Kim, Dae-Seok,Kim, Kyung-Hee,Yoo, Byong Chul,Kim, Seok-Hyun,Gong, Young-Dae,Kim, Soo-Youl The Federation of American Societies for Experimen 2013 The FASEB Journal Vol.27 No.9

<P>Renal cell carcinoma (RCC), the predominant form of kidney cancer, is characterized by high resistance to radiation and chemotherapy. This study shows that expression of protein cross-linking enzyme transglutaminase 2 (TGase 2) is markedly increased in 7 renal cell carcinoma (RCC) cell lines in comparison to HEK293 and other cancer cell lines, such as NCI 60. However, the key role of TGase 2 in RCC was not clear. The down-regulation of TGase 2 was found to stabilize p53 expression, thereby inducing a 3- to 10-fold increase in apoptosis for 786-O, A498, CAKI-1, and ACHN cell lines by DAPI staining. MEF cells from TGase 2<SUP>−/−</SUP> mice showed stabilized p53 under apoptotic stress to compare to MEFs from wild-type mice. TGase 2 directly cross links the DNA binding domain of p53, leading to p53 depletion <I>via</I> autophagy in RCC. TGase 2 and p53 expression showed an inverse relationship in RCC cells. This finding implies that induced expression of TGase 2 promotes tumor cell survival through p53 depletion in RCC.—Ku, B.M., Kim, D.-S. Kim, K.-H., Yoo, B.C., Kim, S.-H., Gong, Y.-D., Kim, S.-Y. Transglutaminase 2 inhibition found to induce p53 mediated apoptosis in renal cell carcinoma.</P>

레이저 빔 응용 기술

윤경구(Kyung Ku Yoon),이성국(Sung Kuk Lee),김재구(Jae Gu Kim),신보성(Bo Sung Shin),최두선(Doo Sun Choi),황경현(Kyung Hyun Whang),박진용(Jin Yong Park) Korean Society for Precision Engineering 2000 한국정밀공학회지 Vol.17 No.7

제2형 당뇨병환자에서 유산소 운동 강도에 따른 인슐린저항성의 변화

구윤희 ( Yun Hyi Ku ),구보경 ( Bo Kyung Koo ),안희정 ( Hee Jung Ahn ),정지연 ( Ji Yun Jeong ),석희금 ( Hee Geum Seok ),김호철 ( Ho Chul Kim ),한경아 ( Kyung Ah Han ),민경완 ( Kyung Wan Min ) 대한당뇨병학회 2009 Diabetes and Metabolism Journal Vol.33 No.5

연구배경: 운동은 주로 동맥경화증 및 인슐린저항성으로부터 보호하는 작용을 통해 사망률을 감소시키는 데에 도움이 되는 것으로 알려져 있다. 이번 연구에서는 제2형 당뇨병환자에서 어떤 강도의 운동이 염증을 완화하고, 내피 세포 기능을 개선시키며 인슐린저항성을 호전시키는 데에 더욱 도움이 되는지 알아보고자 하였다. 방법: 총 59명의 비만한 여성 2형 당뇨병환자가 대조군(CG, N=18), 중강도 운동군(MEG, N=17), 고강도 운동군(VEG, N=14)으로 randomly 배정되어, 가속계로 모니터하며 12주간 운동 요법을 시행하였다. 연구시작시점, 12주 운동 종료 시점, 연구시작으로부터 1년 시점에 체중, 전산화 단층 촬영을 이용하여 복부지방, 피하지방, 내장지방을 측정하였으며, 혈장 hs-CRP, IL-6, 인슐린감수성을 측정하였다. 결과: 평균 연령은 54±7세, 체질량지수는 26.9±2.5 kg/m2이었다. 운동 요법 동안 두 운동군은 운동 칼로리로 각각 488.6±111.9 kcal/day, 518.8±104.1 kcal/day를 사용하였다. 체질량지수, 복부지방, 피하지방은 두 운동군에서(ΔBMI: -1.1±0.7, -0.8±0.5, ΔTF: -4,647±3,613 mm2, -2,577±2,872 mm2, ΔSF: -2,057±2,021 mm2, -1,141±1,825 mm2, respectively) 대조군에 비해 유의하게 감소하였으나(P<0.01), hs-CRP, IL-6 및 FMD는 변화 없었다. 인슐린감수성은 고강도 운동군에서만 감소하였으며, 내장지방은 중강도 운동군에서만 감소하였다. 1년 추적관찰 결과, 이러한 차이는 모두 소실되는 경향을 보였다. 결론: 제2형 당뇨병환자에서 체중 및 체지방 감소를 동반한 고강도 운동은 3개월간의 intervention을 통해 전신 염증수치나 내피 세포 기능의 변화 없이 인슐린감수성 개선 효과를 나타냈다. Background: Exercise offers protection against atherosclerosis and insulin resistance. We evaluated the benefits of exercise at different levels of intensity for ameliorating inflammation, endothelial dysfunction, and insulin resistance in a sample of type 2 diabetic subjects. Methods: Fifty-nine overweight women with type 2 diabetes were randomly assigned to control (CG, N=18), moderate-intensity exercise (MEG, N=17), and vigorous-intensity exercise (VEG, N=14) groups. Patients in the two experimental groups completed a 12-week exercise program, with their exercise activities monitored by accelerometers. We assessed the patients` body weights, total abdominal fat (TF), subcutaneous fat (SF) and visceral fat (VF) via computed tomography, measurements of plasma levels of hs-C-reactive protein (hs-CRP) and interleukin-6 (IL-6), assessment of endothelial function by brachial artery flow-mediated dilation (FMD), and evaluation of insulin sensitivity by insulin tolerance tests, at baseline, at the end of the 12-week interventions, and one year after initiation of the study. Results: At baseline, the average age of all subjects was 54±7 years, and average body mass index (BMI) was 26.9±2.5 kg/m2. During the intervention, patients in the MEG and VEG groups expended comparable amounts of activity-related calories (488.6±111.9 kcal/day, 518.8±104.1 kcal/day, respectively). Although BMI, TF, and SF decreased similarly in the MEG and VEG groups (ΔBMI: -1.1±0.7, -0.8±0.5, ΔTF: -4,647±3,613 mm2, -2,577±2,872 mm2, ΔSF: -2,057±2,021 mm2, -1,141±1,825 mm2, respectively), compared to control (P<0.01), hs-CRP, IL-6, and FMD remained constant in both exercise groups even after completion of the 12-week exercise intervention. Insulin sensitivity improved only in patients subjected to vigorous exercise (VEG). Visceral fat loss was observed only in patients subjected to moderate exercise (MEG). At one-year follow up, these values had all returned to baseline. Conclusion: Exercise vigorous enough to result in significant weight and fat reduction did not ameliorate inflammation and endothelial dysfunction as measured at the end of a 12-week exercise intervention, nor did it result in sustained improvements in insulin sensitivity in type 2 diabetic subjects. (Korean Diabetes J 33:401-411, 2009)

The Effects of Transplanting Time and Meteorological Change to Variation of Phyllochron of Rice

Ku, Bon-Il,Choi, Min-Kyu,Kang, Shin-Ku,Lee, Kyung-Bo,Park, Hong-Kyu,Park, Tae-Seon,Ko, Jae-Kwon,Lee, Byun-Woo The Korean Society of Crop Science 2010 Korean journal of crop science Vol.55 No.3

This study was performed at Rice and Winter Cereal Crops Department of NICS during 2007 and 2008 to investigate the characteristics of rice leaf emergence and to obtain basic data which can be used for rice growth simulation model by which we can forecast rice growth stage and heading date accurately under different cultivars, transplanting date, and climatic conditions. To confirm leaf emergence rate according to rice maturing ecotype, we surveyed the leaf emergence rate and heading date of Unkwangbyeo, Hwayoungbyeo and Nampyeongbyeo which are early maturing, medium maturing and medium-late maturing cultivars, respectively, according to seedling raising duration and transplanting time. When seedling duration was 15 days, the growth duration between transplanting time and completion of flag leaf emergence on main culm were 51.5~78.3 days in Unkwangbyeo, 55.3~87.9 days in Hwayoungbyeo and 58.4~98.4 days in Nampyeongbyeo, respectively. When seedling duration was 30 days, they were 50.1~75.5 days in Unkwangbyeo, 52.4~84.7 days in Hwayoungbyeo and 56.4~93.8 days in Nampyeongbyeo, respectively. As transplanting time delayed, the emerged leaf number after transplanting decreased in all rice cultivars. The cumulative temperature between transplanting time to completion of flag leaf elongation on main culm were $1,281^{\circ}C{\sim}1,650^{\circ}C$ in Unkwangbyeo, $1,344^{\circ}C{\sim}1,891^{\circ}C$ in Hwayoungbyeo and $1,454^{\circ}C{\sim}2,173^{\circ}C$ in Nampyeongbyeo, respectively. Leaf emergence rate on main culm were precisely represented by equation, y = $y_0$ + a / [1 + exp( - (x - $x_0$) / b)]^c, when we used daily mean temperature as variable.

P269 : Nevus comedonicus combined with trichilemmal cyst

( Bo Kyung Kim ),( Dong In Keum ),( Myung Soo Jun ),( Sung Ku Ahn ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.1

Caffeine inhibits cell proliferation and regulates PKA/GSK3β pathways in U87MG human glioma cells

Ku, Bo Mi,Lee, Yeon Kyung,Jeong, Joo Yeon,Ryu, Jinhyun,Choi, Jungil,Kim, Joon Soo,Cho, Yong Woon,Roh, Gu Seob,Kim, Hyun Joon,Cho, Gyeong Jae,Choi, Wan Sung,Kang, Sang Soo Springer-Verlag 2011 Molecules and cells Vol.31 No.3

Decrease of ceramides with ultra long-chain fatty acids in psoriasis model

( Bo-kyung Kim ),( Hee Seok Seo ),( Jong Cheol Shon ),( Kwang-hyeon Liu ),( Jong-won Lee ),( Sung Ku Ahn ),( Seung Phil Hong ) 한국피부장벽학회 2020 한국피부장벽학회지 Vol.22 No.2

Perturbed skin barrier function is the key features of psoriasis. As ceramides(CERs) with long-chain fatty acids(FAs) in the stratum corneum(SC) is important for the skin barrier functions, robust skin barrier can be expected when the process of fatty acid elongation is properly carried out. Here, we aimed to identify the alteration of FA chain length of CERs and lipid synthetic enzymes including elongases(ELOVLs) on the imiquimod(IMQ)-induced psoriasis murine model, human psoriasis skin and in-vitro cell culture. And we also investigated whether IFN-γ may induce the alteration of various enzymes and transcription factors related in biosynthesis of long-chain CERs. Psoriasis-like murine model were induced in BALB/c mice with topical applications of IMQ for 5 days. Human stratum corneum(SC) were collected from 12 subjects with psoriasis. To examine the alteration of FA lengths of CERs, each species of CERs were quantified in of the control and IMQ-treated mice by using LC-MS. We found that portion of free CERs with C26 FA moieties was significantly decreased in IMQ-treated group. And this was also obvious in human SC with psoriasis. We also found that free CERs with C26 or longer FAs and bound CERs C35 or longer FA were significantly decreased suggesting that profiles of FA lengths of CERs shifted toward shorter chain in IMQ-treated murine model. When, we investigate various enzymes affecting the alteration of FA lengths by using quantitative RT-PCR, the expression of ELOVL1, ELOVL4, CerS3 were downregulated whereas ELOVL3 were upregulated in IMQ-treated group. These enzymatic changes may cause impairment in synthesis of CERs with ultra long-chain FAs. We further investigated the role of IFN-γ, a prominent cytokine in psoriasis on various factors involved in FA elongation of the CERs. After normal human epidermal keratinocytes(NHEKs) were stimulated with IFN-γ, expression of ELOVL1, ELOVL4 and CerS3 were significantly reduced. Also, the expression of PPARs, LXR and SREBPs, important transcriptional regulators of ELOVLs and CerSs, were significantly reduced in IFN-treated NHEKs. Thus, we suggest that the expression of factors critical to epidermal differentiation and lipid synthesis is downregulated by IFN-γ in psoriasis, which may affect ceramide chain length and then barrier function.

Caffeine Inhibits Cell Proliferation and Regulates PKA/GSK3β Pathways in U87MG Human Glioma Cells

Bo Mi Ku,Yeon Kyung Lee,Joo Yeon Jeong,Jinhyun Ryu,Jungil Choi,Joon Soo Kim,Yong Woon Cho,Gu Seob Roh,Hyun Joon Kim,Gyeong Jae Cho,Wan Sung Choi,Sang Soo Kang 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.3

Caffeine is the most commonly ingested methylxanthine and has anti-cancer effects in several types of cancer. In this study, we examined the anti-cancer effects of caffeine on gliomas, both in vitro and in vivo. In vitro, caffeine treatment reduced glioma cell proliferation through G_0/G_1-phase cell cycle arrest by suppressing Rb phosphorylation. In addition, caffeine induced apoptosis through caspase-3activation and poly(ADP-ribose) polymerase (PARP) cleavage. Caffeine also phosphorylated serine 9 of glycogen synthase kinase 3 beta (GSK3β). Pretreatment with H89, a pharmacological inhibitor of protein kinase A (PKA), was able to antagonize caffeine-induced GSK3β^(ser9) phosphorylation,suggesting that the mechanism might involve a cAMP-dependent PKA-dependent pathway. In vivo, caffeine-treated tumors exhibited reduced proliferation and increased apoptosis compared with vehicle-treated tumors. These results suggest that caffeine induces cell cycle arrest and caspase-dependent cell death in glioma cells,supporting its potential use in chemotherapeutic options for malignant gliomas.

P110 : Analysis of Baumann skin type in Korean women

( Bo Kyung Kim ),( Myung Soo Jun ),( Sung Jay Choe ),( Seong Jin Kim ),( Sang Hoon Lee ),( Seung Phil Hong ),( Pok Kee Min ),( Hyung Joo Kim ),( Bak Hana ),( Sung Ku Ahn ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: Baumann skin type which was developed by Dr. Baumann could divide skin type into Oily/Dry(O/D), Sensitive/Resistant(S/R), Pigmented/Non-pigmented(P/N), Tight/ Wrinkled(T/W). There were total of 16 types. It could easily provide the comprehensive information about skin type to both physician and patients. Objectives: We aimed to analyze the Baumann skin type in Korean women and evaluate various factors that affect the skin type. Methods: We investigated 560 Korean women aged between 20 and 59. The subjects completed the questionnaire on four categories(O/D, S/R, P/N, T/W). Results: The most prevalent type was ‘OSNT’ and this was followed by DSNT, DRNT and ORNT. The number of non-pigmented, tight and sensitive type is 2.8 fold, 2.3 fold and 1.8 fold larger than pigmented, wrinkle and resistant type. The oily type was more prevalent in group of young age, whereas dry type is dominant in old age group. The portion of pigmented and wrinkled type increased as the subjects became older. Conclusion: This is the first study on investigation of Baumann skin type in Korean women. Most Korean women have sensitive, non-pigmented and tight skin. The oily type is dominant in young women. By using Baumann skin type, we could recommend the appropriate skin care method to patients. In addition, patients could choose the proper skin care products on their own.