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아데노이드 및 구개편도 절제술이 비저항과 비강 내 Geometry에 미치는 영향
나기상,박찬희,구본석,김영덕,김관호,박용호 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2
수술 전 최소단면적이나 비강 부피는 아데노이드나 구개편도의 비대 정도와 상관관계를 보이지 않았으나 비저항은 아데노이드 비대 정도와 유의한 상관관계를 보였다. 구개편도 및 아데노이드 절제술 후 코막힘과 구호흡 증상의 유의한 호전을 보였다. 수술 후 비강의 최소단면적과 부피 등 nasal geometry는 통계적으로 유의한 변화가 없었으나 비저항은 수술 후 유의한 감소를 보였으며 수술 후 비저항의 감소 정도는 아데노이드 비대 정도와 유의한 상관 관계를 보였다 . 이상의 결과로 아데노이드와 구개편도의 비대를 보이는 소아에서 코막힘 증상을 일으키는 데는 아데노이드 비대가 주요 원인이 되며 주로 비강의 후방에 위치한 아데노이드의 mass effect에 의할 것으로 생각한다. The aim of this study was to determine whether adenoid or tonsillar hypertrophy and subsequent adenoidectomy and tonsillectomy affect pediatric nasal airway resistance and nasal geometry and to evaluate the relationships between the degree of hypertrophy and nasal airway resistance or nasal geometry. Thirty-two children, aged 3 to 15 years, selected for adenoidectomy and tonsillectomy due to chronic nasal obstruction and mouth breathing were enrolled. The size of adenoid was evaluated by cephalometric radiograph. The degree of tonsillar hypertrophy was graded clinically. Nasal airway resistance and nasal geometry was evaluated by active anterior rhinomanometry and acoustic rhinometry respectively. These measurements were repeated one month and three months post-operatively. The size of adenoid was found well correlated to preoperative nasal airway resistance. However, there were no correlations between the degree of tonsillar hypertrophy and preoperative nasal airway resistance or nasal geometry. Nasal geometry was not changed after operation. However, nasal airway resistance was reduced significantly at 3 months after operation and the size of adenoid was found well correlated to postoperative changes of nasal airway resistance.
김민지 ․ 구덕본 대구대학교 산업기술연구소 2018 産業技術硏究 Vol.29 No.2
Production of transgenic animals are being used in various fields such as commercial application, human diseases model and drug discovery. One of these animals production method of transgenic animal is direct transfer by pronuclear microinjection. However, the production efficiency of transgenic animals using the pronuclear microinjection is remarkably low. In addition, appropriate conditions such as improving the embryo developmental competence and high quality of blastocysts for the production of transgenic embryos are being required, but studies for these process have not been reported. Therefore, this study investigated the embryonic developmental competence and quality of blastocysts after pronuclear microinjection by using cytochalasin B (CB) or/and melatonin. Cytochalasin B (CB) is important to play role in microinjection by making smoother of the zygote cytoplasm, as well as melatonin is known to improve the embryonic developmental competence and embryos quality. First, we performed the microinjection using injection medium with 10 µg/ml CB and cultured the microinjected zygotes in 0.1 µM melatonin treated within culture medium. Next, we confirmed the DNA damage of microinjected blastocysts using immunofluorescence staining with RAD51 (DNA repairs detection protein) and H2AX139ph (DNA damage detection protein). CB treated group significantly increased blastocyst development rate compared with CB non-treated group (P < 0.05; 25.0 ± 7.2% vs. 17.0 ± 1.3%). After microinjection under the CB treatment, blastocyst development rate and formation of expanded blastocysts were higher in melatonin treated group than those of melatonin non-treated group (29.7 ± 8.3% vs. 24.4 ± 7.7%). In addition, melatonin treated group increased the numbers of RAD51 positive cells compared with non-treated group (P < 0.05). Taken together, these results suggest that CB and melatonin treatment have positive effects for improving the production efficiency of transgenic embryos.
Koo, Deog-Bon,Chae, Jung-Il,Kim, Ji-Su,Wee, Gabbine,Song, Bong-Seok,Lee, Kyung-Kwang,Han, Yong-Mahn Wiley Subscription Services, Inc., A Wiley Company 2005 Molecular reproduction and development Vol.72 No.4
<P>This study was conducted to examine the activities of maturation-promoting factor (MPF) and mitogen-activated protein (MAP) kinase in the porcine oocytes after artificial activation. To determine optimal electrical activation condition, oocytes were exposed to single DC pulse in a variety of electric field strengths (120, 150, 180, and 210 V/mm) and pulse durations (15, 30, 45, and 60 µsec). After the artificial activation, 40–50 oocytes were cultured in a 50 µl drop of NCSU23 medium supplemented with 0.4% BSA at 39°C, 5% CO<SUB>2</SUB> in air for 6 days. No difference was detected in the preimplantation development of pocine oocytes and the mean nuclei number of blastocysts between electric field strengths. Under the 180 V/mm electric field strength, short pulse durations (15 and 30 µsec) showed a higher preimplantation developmental rate of the oocytes and mean nuclei number of blastocysts than an extended electric pulse (60 µsec) (P < 0.05). Single electrical stimulus (180 V/mm, 15 µsec) resulted in higher preimplantation development of porcine oocytes as compared to other chemical stimulators (P < 0.01). Western blot analyses showed the decrease of MPF and MAP kinase in the electrically-activated oocytes. After single electrical stimulus, the amounts of both cdc2 and ERK in porcine oocytes were remarkably reduced by 4 hr and then further decreased by 8 hr. However, the chemically-stimulated oocytes did not show any significant change at the levels of MPF and MAP kinase. Our results indicate that the optimal single electrical pulse is effective on the inactivation of MPF and MAP kinase, eventually leading to the parthenogenetic development of porcine oocytes. Mol. Reprod. Dev. © 2005 Wiley-Liss, Inc.</P>
Effect of Ovarian Extract on Oocyte Maturation and Early Embryonic Development in Pigs
Seul-Gi Yang(Seul-Gi Yang),Jae-Hun Choi(Jae-Hun Choi),Young-Seo Jo(Young-Seo Jo),Ye-Won Kim(Ye-Won Kim),Dong-Mok Lee(Dong-Mok Lee),Hyo-Jin Park(Hyo-Jin Park),Deog-Bon Koo(Deog-Bon Koo) 한국동물보건학회 2022 한국동물보건학회지 Vol.1 No.2
Various factors in the ovary are known to regulate oocyte maturation and hormone secretory functions; however, the effect of ovarian extract (OE) on oocyte maturation and embryonic development in pigs remains unknown. In this study, we first evaluated whether OE supplementation in the in vitro maturation (IVM) medium alters the oocyte maturation capacity by affecting glucose/amino acid metabolites, meiotic maturation, cumulus cell (CC) expansion, and antioxidants. Various OE concentrations (50, 100, 200, 500, and 5000 μg/mL) were included in the IVM medium. Only the oocytes treated with 100 μg/mL OE exhibited an improved meiotic maturation rate when compared with that of the other groups (non-treated group, 78.6 ± 3.0% vs. 100 μg/mL OE-treated group, 81.6 ± 4.3%); however, the difference was not significant. To observe the changes in glucose and amino acid metabolism in the OE-treated oocytes, we measured the amounts of diverse constituents (glucose, lactate, glutamine, and ammonia) in the IVM medium containing OE. Lactate and ammonia levels in the OE-treated group after 44 h of IVM were higher (p < 0.01) than those in the non-treated group. In addition, the expression of the CC expansion factors (Has2 and Tnfaip6) significantly increased (p < 0.05), whereas the mRNA expression levels of antioxidative enzymes (Sod1, Cat, and Gpx1) significantly diminished (p < 0.05) in the OE-treated group. Moreover, mature oocytes treated with 100 μg/mL OE demonstrated increased subsequent embryonic development rates after 144 h of IVM. Thus, the addition of OE in IVM mediums may improve oocyte maturation capacity which could enhance antioxidative enzyme activation, energy metabolism, and expression of the CC expansion factors in porcine oocytes.
Inheritable Histone H4 Acetylation of Somatic Chromatins in Cloned Embryos
Wee, Gabbine,Koo, Deog-Bon,Song, Bong-Seok,Kim, Ji-Su,Kang, Man-Jong,Moon, Seung-Ju,Kang, Yong-Kook,Lee, Kyung-Kwang,Han, Yong-Mahn American Society for Biochemistry and Molecular Bi 2006 The Journal of biological chemistry Vol.281 No.9