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Lee, H. G,P. Y. Lee,Y. K. Lee,S. J. Kim,H. K. Chung,M. K. Seo,J. K. Park,K. S. Min,W. K. Chang 한국동물생명공학회(구 한국동물번식학회) 2003 Reproductive & developmental biology Vol.27 No.4
The effects of additions/deletions in glycosylated residues of recombinant human EPO (rhEPO) produced in CHO-K1 on their secretion were examined. hEPO cDNA was amplified from human liver mRNA and cloned into the pCR2.1 TOPO. Using overlapping-extension site-directed mutagenesis method, glycosylation sites at 24th, 38th, 83rd, and 126th were respectively or accumulatively removed by substituting its asparagine (or serine) with glutamine. To add novel glycosylation sites, 69 and 105th leucine was mutated to asparagine. Mutant and wild type rhEPO constructs were cloned into the pcDNA3 expression vector with CMV promoter and transfected into CHO cell line, CHO-K1, to produce mutant rhEPO mutant rhEPO proteins. Enzyme-linked immunosorbant assay (ELISA) and Western analysis with monoclonal anti-EPO antibody were performed using supernatants of the cultures showing transient and stable expressions respectively. Addition of novel glycosylation reduced rhEPO secretion dramatically while deletion mutants had little effect except some double deletion mutants (△24/83 and △38/83) and triple mutant (△24/38/83). This fact suggests that not single but combination of changes in glycosyl groups affect secretion of rhEPO in cell culture, possibly via changes in their conformations.
Synthesis and Thermoelectric Properties of Ce1−z Pr z Fe4−x Co x Sb12 Skutterudites
Song, K. M.,Shin, D. K.,Jang, K. W.,Choi, S. M.,Lee, S.,Seo, W. S.,Kim, I. H. Springer Science + Business Media 2017 Journal of electronic materials Vol.46 No.5
<P>p-Type Ce1-z Pr (z) Fe4-x Co (x) Sb-12 skutterudites were prepared by encapsulated melting, quenching, annealing, and hot pressing. While the skutterudite phase was successfully synthesized, a small amount of the secondary phase (FeSb2) was observed. According to the scanning electron microscope analysis, (Ce,Pr)Sb-2 phases were also observed for Co-substituted specimens (x = 0.5). The electrical conductivity decreased with increasing temperature, implying a degenerate semiconductor behavior, and also decreased with increasing Co contents. All specimens showed p-type characteristics having positive signs of the Hall coefficient and the Seebeck coefficient. The Seebeck coefficient increased with increasing temperature and reached a maximum value at 823 K. The power factor (PF) increased with decreasing Co content and Ce0.75Pr0.25 Fe4Sb12 showed a peak value of PF = 3.2 mW m(-1) K-2 at 823 K. The electronic thermal conductivity decreased with increasing Co contents and the lattice thermal conductivity decreased with decreasing Ce and Co contents at high temperature. The thermal conductivity increased at temperatures above 623 K due to bipolar conduction. The dimensionless figurea of pound merit (ZT) showed a maximum value of ZT = 0.84 at 823 K for Ce0.25Pr0.75Fe4Sb12.</P>
남기우(K.W. Nam),문희준(H.J. Moon),박상현(S.H. Park),김은선(E.S. Kim),서현수(H.S. Seo),김종순(J.S. Kim) 대한기계학회 2009 대한기계학회 춘추학술대회 Vol.2009 No.11
Zinc coatings are widely used in practice for preventing the corrosion of steel. But the zinc coating can be involved by corrosion phenomena with the consequence that some steel surface is unprotected. The reduction of the corrosion rate of zinc is an important topic. In the past a very popular way to reduce the corrosion rate of zinc was the use of chemical conversion layers based on Cr??. However, there is the important problem that the use of chromium salts is now restricted because of environmental protection legislation. Therefore very important to develop new zinc surface treatments environmentally friendly to improve the corrosion resistance of zinc and the adhesion with the final organic protective layer. In this study, Urethane solution (only Urethane 20 % ; S-700) and organic/inorganic solution with Si (Si polysilicate 10 % + Urethane 6 % + hardner 4 % ; LRO317-1)are used. Based on the salt spray test of 72h, S-700 and LRO317-1 had a superior effect on EGI and HGI, respectively.