Exosome-mediated lnc-ABCA12-3 promotes proliferation and glycolysis but inhibits apoptosis by regulating the toll-like receptor 4/nuclear factor kappa-B signaling pathway in esophageal squamous cell carcinoma

Junliang Ma,Yijun Luo,Yingjie Liu,Cheng Chen,Anping Chen,Lubiao Liang,Wenxiang Wang,Yongxiang Song 대한약리학회 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.1

Esophageal squamous cell carcinoma (ESCC) is a kind of malignant tumor with high incidence and mortality in the digestive system. The aim of this study is to explore the function of lnc-ABCA12-3 in the development of ESCC and its unique mechanisms. RT-PCR was applied to detect gene transcription levels in tissues or cell lines like TE-1, EC9706, and HEEC cells. Western blot was conducted to identify protein expression levels of mitochondrial apoptosis and toll-like receptor 4 (TLR4)/nuclear factor kappa-B (NF-κB) signaling pathway. CCK-8 and EdU assays were carried out to measure cell proliferation, and cell apoptosis was examined by flow cytometry. ELISA was used for checking the changes in glycolysis-related indicators. Lnc-ABCA12-3 was highly expressed in ESCC tissues and cells, which preferred it to be a candidate target. The TE-1 and EC9706 cells proliferation and glycolysis were obviously inhibited with the downregulation of lnc-ABCA12-3, while apoptosis was promoted. TLR4 activator could largely reverse the apoptosis acceleration and relieved the proliferation and glycolysis suppression caused by lnc-ABCA12-3 downregulation. Moreover, the effect of lnc-ABCA12-3 on ESCC cells was actualized by activating the TLR4/NF-κB signaling pathway under the mediation of exosome. Taken together, the lnc-ABCA12-3 could promote the proliferation and glycolysis of ESCC, while repressing its apoptosis probably by regulating the TLR4/NF-κB signaling pathway under the mediation of exosome.

The enumeration of rooted cubic c-nets$^{\dag}$

Junliang Cai,Rongxia Hao,Yanpei Liu 한국전산응용수학회 2005 Journal of applied mathematics & informatics Vol.18 No.1-2

This paper is to establish a functional equation satisfied by the generating function for counting rooted cubic c-nets and then to determine the parametric expressions of the equation directly. Meanwhile, the explicit formulae for counting rooted cubic c-nets are derived immediately by employing Lagrangian inversion with one or two parameters. Both of them are summation-free and in which one is just an answer to the open problem (8.6.5) in [1].

Optimized Iterative Algorithm for Energy-Efficient Power Allocation in Two-tier Heterogeneous Networks

Wang Junliang,Li Wenjia,Liu Haitao 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.12

In heterogeneous networks, the issue of interference between femtocells and macrocells should be carefully considered. Resource allocation schemes with cognitive technologies have been a key challenge to manage interference. In this paper, we investigate price-based power allocation strategies with the energy efficiency criterion for a spectrum-sharing heterogeneous cognitive network from the aspect of energy efficiency, and provide the utility function of macrocell and femtocells based on a non-cooperative Stackleberg game model. We build a combination of price vector and power allocation values by standard Lagrangian method and propose an improved iteration algorithm based on price updating to obtain the Stackleberg equilibrium solution. The simulation results verify the proposed method can improve energy efficiency and achieve better utility.

The role of exosomal miR-181b in the crosstalk between NSCLC cells and tumor-associated macrophages

Ma Junliang,Chen Shaolin,Liu Yingjie,Han Hao,Gong Ming,Song Yongxiang 한국유전학회 2022 Genes & Genomics Vol.44 No.10

Background: It has been reported that tumor-associated macrophages (TAMs) participate in modulating the progression of cancer in the tumor microenvironment. However, the crosstalk between TAMs and non-small cell lung cancer (NSCLC) is still unclear. Objective: We investigated whether NSCLC-derived exosomes could affect TAMs, which feedback modulated progression of NSCLC. Methods: MiR-181b expression was measured by RT-PCR. Human THP-1 monocyte was differentiated into macrophages with phorbol myristate acetate, which were further identified by transmission electron microscopy and western blot. Macrophage M1 and M2 polarizations were detected by flow cytometry, RT-PCR and western blot. Proliferation, migration, and invasion of NSCLC cells treated with conditioned mediums were detected by EdU and Transwell assays. Results: We demonstrated that miR-181b was up-regulated in exosomes derived from NSCLC patients' serum and NSCLC cells. MiR-181b could be transferred to macrophages via exosomes in the co-culture system of macrophages and NSCLC cells, which promoted macrophage M2 polarization. Further examinations revealed that exosomes derived from NSCLC cells could enhanced macrophage M2 polarizations by regulating miR-181b/JAK2/STAT3 axis, and silencing miR-181b in NSCLC cells and JAK2 inhibitor used in macrophages could reverse the effects. Importantly, the conditioned medium of macrophages treated with NSCLC cell-derived exosomes could promote NSCLC cell proliferation, migration, and invasion. Silencing miR-181b in NSCLC cells and JAK2 inhibitor used in macrophages could block the effects. Conclusions: All of these results indicated that exosomal miR-181b participated in the crosstalk between NSCLC cells and TAMs, providing potential therapeutic targets for NSCLC.

A fuzzy controller based on incomplete differential ahead PID algorithm for a remotely operated vehicle

Cao, Junliang,Yin, Hanjun,Liu, Chunhu,Lian, Lian Techno-Press 2013 Ocean systems engineering Vol.3 No.3

In many applications, Remotely Operated Vehicles (ROVs) are required to be capable of course keeping, depth keeping, and height keeping. The ROV must be able to resist time-variant external forces and moments or frequent manipulate changes in some specified circumstances, which require the control system meets high precision, fast response, and good robustness. This study introduces a Fuzzy-Incomplete Derivative Ahead-PID (FIDA-PID) control system for a 500-meter ROV with four degrees of freedom (DOFs) to achieve course, depth, and height keeping. In the FIDA-PID control system, a Fuzzy Gain Scheduling Controller (FGSC) is designed on the basis of the incomplete derivative ahead PID control system to make the controller suitable for various situations. The parameters in the fuzzy scheme are optimized via many cycles of trial-and-error in a 10-meter-deep water tank. Significant improvements have been observed through simulation and experimental results within 4-DOFs.

COUNING g-ESSENTIAL MAPS ON SURFACES WITH SMALL GENERA

Rongxia Hao,Junliang Cai,Yanpei Liu 한국전산응용수학회 2002 Journal of applied mathematics & informatics Vol.9 No.2

This paper provides some functional equations and parametric expressions of {\t$g$}-essential maps on the projective plane, on the torus and on the Klein bottle with the size as a parameter and gives their explicit formulae for exact enumeration further. This paper provides some functional equations and parametric expressions of {\t$g$}-essential maps on the projective plane, on the torus and on the Klein bottle with the size as a parameter and gives their explicit formulae for exact enumeration further.

COUNING g-ESSENTIAL MAPS ON SURFACES WITH SMALL GENERA

Hao, Rongxia,Cai, Junliang,Liu, Yanpel 한국전산응용수학회 2002 The Korean journal of computational & applied math Vol.9 No.2

This paper provides some functional equations and parametric expressions of f-essential maps on the projective plane, on the torus and on the Klein bottle with the size as a parameter and gives their explicit formulae for exact enumeration further.

The earth mover’s distance and Bayesian linear discriminant analysis for epileptic seizure detection in scalp EEG

Shasha Yuan,Jinxing Liu,Junliang Shang,Xiangzhen Kong,Qi Yuan,Zhen Ma 대한의용생체공학회 2018 Biomedical Engineering Letters (BMEL) Vol.8 No.4

Since epileptic seizure is unpredictable and paroxysmal, an automatic system for seizure detecting could be of greatsignificance and assistance to patients and medical staff. In this paper, a novel method is proposed for multichannel patientspecificseizure detection applying the earth mover’s distance (EMD) in scalp EEG. Firstly, the wavelet decomposition isexecuted to the original EEGs with five scales, the scale 3, 4 and 5 are selected and transformed into histograms andafterwards the distances between histograms in pairs are computed applying the earth mover’s distance as effectivefeatures. Then, the EMD features are sent to the classifier based on the Bayesian linear discriminant analysis (BLDA) forclassification, and an efficient postprocessing procedure is applied to improve the detection system precision, finally. Toevaluate the performance of the proposed method, the CHB-MIT scalp EEG database with 958 h EEG recordings from 23epileptic patients is used and a relatively satisfactory detection rate is achieved with the average sensitivity of 95.65% andfalse detection rate of 0.68/h. The good performance of this algorithm indicates the potential application for seizuremonitoring in clinical practice.

Visible-light-driven photocatalyst of La–N-codoped TiO2 nano-photocatalyst: Fabrication and its enhanced photocatalytic performance and mechanism

Junjing Li,Bo Li,Jiejing Li,Junliang Liu,Liang Wang,Hongwei Zhang,Zhaohui Zhang,Bin Zhao 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.25 No.-

In this study, La, N codoped TiO2 (La–N–TiO2) nano-photocatalyst was fabricated through simple sol–gel method. The resulting samples were characterized through X-ray diffraction (XRD), scanning electrons microscope (SEM), transmission electrons microscope (TEM), X-ray photoelectron spectroscopy (XPS) and UV–visible light diffuse reflection spectroscopy (UV–vis DRS). Results revealed that La elements were not implanted into the structure of TiO2 but existed as the form of Ti–O–La bonds in the interstitial site, while N dopants were incorporated into the lattice of TiO2 and coexisted in the substitutional N (N– Ti–O) and interstitial N (Ti–O–N) in the La–N–TiO2 catalyst, thereby resulting in the formation of new impurity energy level between the forbidden band and greatly enhancement of light absorption ability in visible light region. The enhanced visible light driven (VLD) photocatalytic (PC) performance for the degradation of phenol aqueous solution could be attributed to the intense light absorbance in visible light region and high separation efficiency of photogenerated charge carriers.

Anaerobic membrane bioreactors for antibiotic wastewater treatment: Performance and membrane fouling issues

Cheng, Dongle,Ngo, Huu Hao,Guo, Wenshan,Liu, Yiwen,Chang, Soon Woong,Nguyen, Dinh Duc,Nghiem, Long Duc,Zhou, Junliang,Ni, Bingjie Elsevier 2018 Bioresource technology Vol.267 No.-

<P><B>Abstract</B></P> <P>Antibiotic wastewater has become a major concern due to the toxicity and recalcitrance of antibiotics. Anaerobic membrane bioreactors (AnMBRs) are considered alternative technology for treating antibiotic wastewater because of their advantages over the conventional anaerobic processes and aerobic MBRs. However, membrane fouling remains the most challenging issue in the AnMBRs’ operation and this limits their application. This review critically discusses: (i) antibiotics removal and antibiotic resistance genes (ARGs) in different types of AnMBRs and the impact of antibiotics on membrane fouling and (ii) the integrated AnMBRs systems for fouling control and removal of antibiotics. The presence of antibiotics in AnMBRs could aggravate membrane fouling by influencing fouling-related factors (i.e., sludge particle size, extracellular polymeric substances (EPS), soluble microbial products (SMP), and fouling-related microbial communities). Conclusively, integrated AnMBR systems can be a practical technology for antibiotic wastewater treatment.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Removal of antibiotics and their effects on membrane fouling in AnMBRs are reviewed. </LI> <LI> AnMBRs are efficient for treating antibiotic wastewater mainly by biodegradation. </LI> <LI> The presence of antibiotics in AnMBRs will aggravate membrane fouling. </LI> <LI> Integrating AnMBRs with biofilm carriers and BES is efficient in fouling control. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>