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정종헌,원휘준,최왕규,김계남,이성호,오원진 한국공업화학회 2003 응용화학 Vol.7 No.1
Liquid wastes containing uranium ions in high concentration of chemical salts havebeen treated by electrosorption on a activated carbon fibers(ACFs) electrode. Effectiveuranium (VI) removal is accomplished when the potential is -0.5V(vs. Ag/AgCl) ormore negative. For a feed concentration of 100mg/1, the concentration of U(VI) in thecell effluent is reduced to less than 1mg/1, and electrosorption capacity over 500mg/uranium/gACF is reached. The adsorbed uranium can be desorbed up to 99%for 20h by passing a 1.9N NaCl solution at pH 3 through the cell and applying a potential of +1.2V. The amount of desorbed uranium increased as the solution pHwas lowered and the applied potential increased.
Choi, Jun-Hui,Kim, Seung,Sapkota, Kumar,Park, Se-Eun,Kim, Sung-Jun Humana Press 2011 Applied biochemistry and biotechnology Vol.165 No.2
<P>Recombinant human platelet-derived growth factor-BB (rhPDGF-BB) is widely used in many therapeutic applications. Until now, there has been no report on rhPDGF-BB expressed in fungi. In this study, we tested whether Pleurotus eryngii could support the expression of human therapeutic rhPDGF-BB protein. A binary vector pCAMBIA1304 containing the hPDGF-BB gene was constructed and introduced into P. eryngii via Agrobacterium tumefaciens-mediated transformation. The transformation of hPDGF-BB gene was confirmed by Southern blot and PCR, whereas the expression was confirmed by Western blot analysis. The recombinant hPDGF-BB reached a maximum expression level of 1.98% of total soluble protein in transgenic mycelia and was in dimeric form. A bioassay revealed that hPDGF-BB expressed in P. eryngii increased proliferation of NIH-3T3 cells similarly to standard material. These results suggest that P. eryngii can be a robust system for the production of human therapeutic proteins including the hPDGF-BB.</P>
Antioxidant and Improvement Effect of Blood Circulation of Wild Vegetables Extracts
Jun-Hui Choi,Se-Eun Park,Hyo-Jeong Lee,Seung Kim 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
The study was investigated on in vitro antioxidant, antithrombotic, and anticoagulation activity of wild vegetables extracts from Ligularia fischeri, Aster scaber Thunb., Ligularia stenocephala, and Petasites japonicus. We isolated the ethanolic extracts from leave of L. fischeri, A. scaber Thunb., L. stenocephala, and P. japonicus. The antioxidant effects of the extracts were assayed by total polyphenol analysis, flavonoid analysis, and radical scavenging assay. The antithrombotic activities of different extracts were examined by fibrin clot assay, APTT, and PT, compared with the properties of the related commercial products. The polyphenolic and flavonoids levels were confirmed in the wild vegetables extracts and the extracts inhibited the human fibrin clot and the inhibitory effect of fibrin clot by the extract was concentration-dependent. The extracts caused the prolongation of coagulation on human blood in APTT and PT assay. In conclusion, these results suggest that these wild vegetables may be good candidates as a natural improvement source for blood circulation.
Choi, Mansoo,Lee, Sang-Hun,Jung, Yang-Il,Jung, Jun-Young,Park, Jung-Soon,Choi, Wang-Kyu,Park, Sang-Yoon,Won, Hui-Jun,Moon, Jei-Kwon,Choi, Jihoon,Kim, Seon-Byeong ELSEVIER SCIENCE 2017 JOURNAL OF ALLOYS AND COMPOUNDS Vol.729 No.-
<P><B>Abstract</B></P> <P>NiFe<SUB>2</SUB>O<SUB>4</SUB> thin film has been prepared by electron beam evaporation method and has been investigated as anode materials providing large reversible Li<SUP>+</SUP> capacity with high cycling performance for lithium ion batteries. The NiFe<SUB>2</SUB>O<SUB>4</SUB> thin film crystallized with annealing process after NiFe<SUB>2</SUB>O<SUB>4</SUB> thin film deposition. In electrochemical measurements, the first discharge and charge capacity of NiFe<SUB>2</SUB>O<SUB>4</SUB> thin film were found to be 1693 mAh g<SUP>−1</SUP> and 1108 mAh g<SUP>−1</SUP>, which is above its theoretical capacity (915 mAh g<SUP>−1</SUP>). In addition, the cycle performance test of NiFe<SUB>2</SUB>O<SUB>4</SUB> thin film also showed steady charge-discharge capacity at 0.1 C during 100 cycles compared to other NiFe<SUB>2</SUB>O<SUB>4</SUB> materials, indicating that the empty space or porous surface of electrode was beneficial for accessibility of electrolyte and served as buffer to alleviate stress.</P> <P><B>Highlights</B></P> <P> <UL> <LI> NiFe<SUB>2</SUB>O<SUB>4</SUB> thin film has been prepared by electron beam evaporation method. </LI> <LI> The first discharge and charge capacity of NiFe<SUB>2</SUB>O<SUB>4</SUB> thin film were found to be 1693 mAh g<SUP>−1</SUP> and 1108 mAh g<SUP>−1</SUP>. </LI> <LI> The cycle performance test of NiFe<SUB>2</SUB>O<SUB>4</SUB> thin film also showed steady charge-discharge capacity at 0.1 C during 100 cycles. </LI> </UL> </P>
Choi, Jun-Hui,Sapkota, Kumar,Kim, Myung-Kon,Kim, Seung,Kim, Sung-Jun Humana Press 2014 Applied biochemistry and biotechnology Vol.173 No.8
<P>A direct-acting fibrinolytic serine protease named undariase possessing anticoagulant and antiplatelet properties was purified from Undaria pinnatifida. Undariase showed a molecular weight of 50?kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry. It displayed a strong fibrin zymogram lysis band corresponding to the same molecular mass. The N-terminal sequence of undariase, LTATTCEELAAAPTD, does not match with any known fibrinolytic enzyme. The enzyme was stable and active at high temperatures (35-70?C). The fibrinolytic activity of undariase was strongly inhibited by phenylmethylsulfonyl fluoride (PMSF) and 4-(amidinophenyl) methanesulfonyl fluoride (APMSF). The K m and V max values for substrate S-2251 were determined as 6.15?mM and 90.91?mM/min/ml, respectively. Undariase resulted in clot lysis by directly cleaving α and β chains of fibrin. Similarly, it preferentially acted on the Aα chain of fibrinogen followed by cleavage of the Bβ chain. It significantly prolonged the PFA-100 closure times of citrated whole human blood. In addition, undariase delayed the coagulation time and increased activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). Undariase exerted a significant protective effect against collagen plus epinephrine-induced pulmonary thromboembolism in mice. It prevented carrageenan-induced thrombus formation in the tail of mice. It also resulted in prolongation of APTT ex vivo. In conclusion, these results suggested a therapeutic potential of undariase for thrombosis.</P>