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Li Deng,Wenpeng Jia,Wen Zheng,Hao Liu,Degang Jiang,Zimeng Li,Yu Tian,Wenling Zhang,Jingquan Liu 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.58 No.-
Ni–Al layered double hydroxides (Ni–Al LDHs) are adopted as an electrorheological (ER) materialascribed to their special layered structure, ample polarizable groups and good dispersibility. Themagnetite nanoparticles (Fe3O4 NPs) are utilized as classical magnetorheological (MR) materials onaccount of their excellent paramagnetic property. In this work, we report the rational design of highperformanceER/MR materials by integrating the structural merits of Fe3O4 NPs onto a binary layeredstructure system. The obtained Fe3O4/Ni–Al LDH NCs dispersed in silicone oil displayed appealing dualER/MR performances. The work opens a new path toward novel stimuli-responsive materials forindustrial applications.
Juan Liu,Jingquan Li,Li Feng,Hui Cao,Zhongli Cui 한국미생물학회 2010 The journal of microbiology Vol.48 No.6
Separation of bacterial cells from soil is a key step in the construction of metagenomic BAC libraries with large DNA inserts. Our results showed that when combined with sodium pyro-phosphate and homogenization for soil dispersion, sucrose density gradient centrifugation (SDGC) was more effective at separating bacteria from soil than was low speed centrifugation (LSC). More than 70% of the cells, along with some soil colloids,were recovered with one round of centrifugation. A solution of 0.8% NaCl was used to resuspend these cell and soil pellets for purification with nycodenz density gradient centrifugation (NDGC). After purification,more than 30% of the bacterial cells in the primary soil were extracted. This procedure effectively removed soil contamination and yielded sufficient cells for high molecular weight (HMW) DNA isolation. Ribosomal intergenic spacer analysis (RISA) showed that the microbial community structure of the extracted cells was similar to that of the primary soil, suggesting that this extraction procedure did not significantly change the the soil bacteria community structure. HMW DNA was isolated from bacterial cells extracted from red soil for metagenomic BAC library construction. This library contained DNA inserts of more than 200 Mb with an average size of 75 kb.
Dynamic characteristics analysis of partial-interaction composite continuous beams
Genshen Fang,Jingquan Wang,Shuai Li,Shubin Zhang 국제구조공학회 2016 Steel and Composite Structures, An International J Vol.21 No.1
The dynamic characteristics of continuous steel-concrete composite beams considering the effect of interlayer slip were investigated based on Euler Bernoulli's beam theory. A simplified calculation model was presented, in which the Mode Stiffness Matrix (MSM) was developed. The natural frequencies and modes of partialinteraction composite continuous beams can be calculated accurately and easily by the use of MSM. Proceeding from the present method, the natural frequencies of two-span steel-concrete composite continuous beams with different span-ratios (0.53, 0.73, 0.85, 1) and different shear connection stiffnesses on the interface are calculated. The influence pattern of interfacial stiffness on bending vibration frequency was found. With the decrease of shear connection stiffness on the interface, the flexural vibration frequencies decrease obviously. And the influence on low order modes is more obvious while the reduction degree of high order is more sizeable. The real natural frequencies of partial-interaction continuous beams commonly used could have a 20% to 40% reduction compared with the fully-interaction ones. Furthermore, the reduction-ratios of natural frequencies for different span-ratios two-span composite beams with uniform shear connection stiffnesses are totally the same. The span-ratio mainly impacts on the mode shape. Four kinds of shear connection stiffnesses of steel-concrete composite continuous beams are calculated and compared with the experimental data and the FEM results. The calculated results using the proposed method agree well with the experimental and FEM ones on the low order modes which mainly determine the vibration properties.
Jianan Qi,Zhao Cheng,Jingquan Wang,Yutong Zhu,Wenchao Li 국제구조공학회 2020 Structural Engineering and Mechanics, An Int'l Jou Vol.75 No.1
This paper presents a full-scale experimental test to investigate the flexural behavior of an innovative dovetail ultra-high performance concrete (UHPC) joint designed for the 5th Nanjing Yangtze River Bridge. The test specimen had a dimension of 3600 × 1600 × 170 mm, in accordance with the real bridge. The failure mode, crack pattern and structural response were presented. The ductility and stiffness degradation of the tested specimens were explicitly discussed. Test results indicated that different from conventional reinforced concrete slabs, well-distributed cracks with small spacing were observed for UHPC joint slabs at failure. The average nominal flexural cracking strength of the test specimens was 7.7 MPa, signifying good crack resistance of the proposed dovetail UHPC joint. It is recommended that high grade reinforcement be cooperatively used to take full advantage of the superior mechanical property of UHPC. A new ductility index, expressed by dividing the ultimate deflection by flexural cracking deflection, was introduced to evaluate the post-cracking ductility capacity. Finally, a strut-and-tie (STM) model was developed to predict the ultimate strength of the proposed UHPC joint.
( Xin Liu ),( Ying Dong ),( Jingquan Wang ),( Long Li ),( Zhenmin-zhong ),( Yun-pan Li ),( Shao-jun Chen ),( Yu-cai Fu ),( Wen-can Xu ),( Chi-ju Wei ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.6
Vesicular stomatitis virus G glycoprotein (VSV-G) has been widely used for pseudotyping retroviral, lentiviral, and artificial viral vectors. The objective of this study was to establish a potential approach for large-scale production of VSV-G. To this end, VSV-G was cloned with an N-terminal His-tag into Pichia pastoris expression vector pPIC3.5K. Three clones (Mut<sup>s</sup>) containing the VSV-G expression cassette were identified by PCR. All clones proliferated normally in expansion medium, whereas the proliferation was reduced significantly under induction conditions. VSV-G protein was detected in cell lysates by western blot analysis, and the highest expression level was observed at 96 h post induction. VSV-G could also be obtained from the condition medium of yeast protoplasts. Furthermore, VSV-G could be incorporated into Ad293 cells and was able to induce cell fusion, leading to the transfer of cytoplasmic protein. Finally, VSV-G-mediated DNA transfection was assayed by flow cytometry and luciferase measurement. Incubation of VSV-G lysate with the pGL3-control DNA complex increased the luciferase activity in Ad293 and HeLa cells by about 3-fold. Likewise, incubation of VSV-G lysate with the pCMV-DsRed DNA complex improved the transfection efficiency into Ad293 by 10% and into HeLa cells by about 1-fold. In conclusion, these results demonstrate that VSV-G could be produced from P. pastoris with biofunctionalities, demonstrating that large-scale production of the viral glycoprotein is feasible.
A novel elsinochrome A derivative: a study of drug delivery and photodynamic activity
Zhang, Yang,Xie, Jie,Zhang, Luyong,Li, Cong,Chen, Hongxia,Gu, Ying,Zhao, Jingquan Korean Society of Photoscience 2009 Photochemical & photobiological sciences Vol.8 No.12
Elsinochrome A (EA) possesses the highest singlet-oxygen quantum yield (0.98) amongst the perilenoquinoid pigments and may be suitable as a phototherapeutic drug. However, there have been virtually no studies into its medicinal applications. Based on the analysis of chemical derivatives of hypocrellins (the same family as EA), 5-(3-mercapto-1-propanesulfonic acid)-substituted elsinochrome A (MPEA) with an amphiphilicity was designed and synthesized by considering drug delivery and biological activity requirements. MPEA possesses a water solubility of 5.1 mg $mL^{-1}$, which is just sufficient to enable dissolution at a clinically acceptable concentration, while its partition coefficient (n-octanol/phosphate buffered saline) of 7 guarantees affinity to biological targets. MPEA could photogenerate semiquinone anion radicals and reactive oxygen species, especially singlet oxygen, at a yield of 0.73, which approaches that for hypocrellin B. Biological tests confirmed that the photodynamic activity of MPEA was as high as 60% of that of its parent EA, which is significantly higher than that of most other photosensitizers.
Xuqiang Ji,Wenling Zhang,Wenpeng Ji,Xiaoxia Wan,Yutian Wang,Li Deng,Jingquan Liu 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.56 No.-
The cactus-like double-shell structured SiO2@TiO2 microspheres (CDS SiO2@TiO2 microspheres) withhigh dielectric properties were successfully fabricated and utilized as high-performance electrorheological(ER)fluids and microwave absorbing materials. The SiO2 nanospheres with average diametersof 190 nm were synthesized by the hydrolysis of tetraethyl orthosilicate (TEOS), and used as the core forthefirst coating layer of TiO2 achieved by sol-gel method. Thefinal cactus-like TiO2 layer was prepared viaa hydrothermal method. The morphology of the resultant samples was characterized via transmissionelectron microscopy (TEM), scanning electron microscopy (SEM) and scanning transmission electronmicroscopy (STEM). This novel CDS SiO2@TiO2 microspheres based suspension exhibited improved ERproperties compared to conventional bare SiO2 spheres.