Combined transcriptomic and proteomic analysis of flubendiamide resistance in Plutella xylostella

Li Jing‐Jing,Jin Ming‐Hui,Wang Nian‐Meng,Yu Qi‐Tong,Shang Ze‐Yu,Xue Chao‐Bin 한국곤충학회 2020 Entomological Research Vol.50 No.10

Diamondback moth (DBM), Plutella xylostella, is an important pest of crucifers worldwide. The extensive use of diamide insecticides has led to DBM resistance in the world, and this presents a serious threat to vegetable production. In the present study, transcriptomic and proteomic analyses were combined to investigate the potential flubendiamide‐resistance mechanism in DBM. The lab‐selected (Rh) and field‐collected (Rb) flubendiamide‐resistant lines of P. xylostella with resistance ratios of 1889.92‐fold and 1250.97‐fold, respectively, were used, as well as a lab‐reared flubendiamide‐susceptible line (S). Compared with the S group, the transcriptomic analysis revealed 151 upregulated and 287 downregulated gene messengers in the Rh group and 432 upregulated and 565 downregulated gene messengers in the Rb group. The most frequently enriched pathways of differentially expressed genes (DEGs) were mainly involved in metabolic pathways. Metabolism related genes, including two P450, two ABC transporters, and three trypsins, were upregulated in the Rh line. Additionally, some P450 genes, trypsin, juvenile hormone (JH), and mucin genes were also upregulated in the Rb line. In proteomic analysis comparisons with the S group, there were 78 upregulated and 90 downregulated proteins in the Rh group and 221 upregulated and 155 downregulated proteins in the Rb group. Further analyses found that three CYP and 11 CYP proteins were over‐expressed in Rh and Rb lines, respectively. Four glutathione S‐transferase (GST) and four UGTs were over‐expressed in Rb line. So, we deduced that the detoxification metabolism may be the main mechanism of flubendiamide resistance in P. xylostella.

朝鮮時期『虞註杜律』的傳入和翻印版本硏究

劉婧 ( Liu Jing ) 중국어문학회 2022 中國語文學誌 Vol.- No.81

WuZhuDuLv(虞註杜律) is a compilation of Du Fu(杜甫)’s Poems which were edited by Yu Ji(虞集) during the Yuan(元) Dynasty of China. WuZhuDuLv(虞註杜律) was first printed by Zhu Xiong(朱熊) of Xuande(宣德, 1434) in the Ming Dynasty, and re-printed by Lin Jing(林靖) during the period of Zhengtong(正統, 1443). Lin Jing’s printed edition was first introduced to Choseon during the period of Chenghua(成化). Over the next four hundred years, WuZhuDuLv(虞註杜律) was reprinted and published several times as a teaching material for the study of Du Fu(杜甫)’s poetry and other Chinese poetry, which had a great influence in Choseon, The book, WuZhuDuLv(虞註杜律) introduced into Choseon, first reprinted by QingzhouMu(淸州牧). It was a book printed from a wooden block. Later reproduced the metal types of Jiayin(甲寅) and Yunge(芸阁). There are many types of reprinted editions and there are also differences between the first and later editions. In the late Choseon, woodblock printed books of the WuZhuDuLv(虞註杜律) were printed the most. This study summarized and analyzed various editions in which the WuZhuDuLv(虞註杜律) was introduced into Choseon from the literature’s perspective. Through this fact, it is possible to understand the various situations in which Du Fu’s poems related literature was printed and distributed in Choseon. And it will be great help to study of publication and dissemination of Du Fu’s poems in the Choseon Dynasty.

Up‑regulation of miR‑27 extenuates lipopolysaccharide‑induced injury in H9c2 cells via modulating ICAM1 expression

Jing‑Fang Xiang,Jian‑Chun Yu,Jian‑You Zhu 한국유전학회 2019 Genes & Genomics Vol.41 No.12

Background MiR-27 has been found to present an overt myocardial expression during cardiogenesis. However, whether miR-27 involves in myocarditis development and the possible molecular mechanism remain unknown. The purpose of this study was to investigate the biological characteristic of miR-27 in LPS-damaged H9c2 cells. Methods H9c2 cells were treated with lipopolysaccharide (LPS, 10 μg/ml) for 12 h to form cell injury. MiR-27 mimic and inhibitor were used to up-regulate or down-regulate miR-27 expression. MTT assay and flow cytometry analysis were conducted to test cell viability and apoptosis. The relative RNA expression level of miR-27 and intercellular adhesion molecule 1 (ICAM1) was determined by qRT-PCR. Luciferase reporter gene assay was utilized to confirm the interaction between miR-27 and ICAM1. Western blot was used to determine the protein expression levels. Results We observed that LPS treatment significantly decreased the level of miR-27 in H9c2 cells. Moreover, LPS exposure suppressed cell viability, promoted cell apoptosis and increased the relative expression of p-NF-κB p65/NF-κB p65 and p-IκBα/IκBα. Up-regulation of miR-27 increased cell proliferation and reduced cell apoptosis, while down-regulation of miR-27 suppressed cell growth and promoted cell apoptosis. ICAM1 was predicted and verified as a target of miR-27, and the expression of ICAM1 is negatively regulated by miR-27. The relative expression of p-NF-κB p65/NF-κB p65 and p-IκBα/ IκBα was dramatically decreased by miR-27 mimic and increased by miR-27 inhibitor. Conclusion Our study illustrated that up-regulation of miR-27 exhibits a protective effect on LPS-damaged H9c2 cells, which may be achieved by regulating ICAM1 and NF-κB signaling.

《诗经》英译情况研究

韩菁(Han,Jing) 조선대학교 국제문화연구원 2017 국제문화연구 Vol.10 No.1

As the first collection of Chinese poems, The Book of Songs collected poems written in about 500 years from the turn of the Shang dynasty and the Zhou dynasty to the middle of the Spring and Autumn. As the top of the Five Classics, it is not only a treasure of the traditional Chinese culture, but also the culture heritage of the world. There are 311 poems in it, which can be divided into three part Feng(Ballads), Ya(Festival Odes) and Song(Sacrificial Songs). It also adopts Fu, Bi, Xing as the technique of expression, sometimes the songs come straight to the point, and sometimes they use symbolism, all of which endow the songs sincere feelings and make them honest and unsophisticated. Being regarded as a quintessential part of our Chinese people’s culture, it has been the focus of attention from the very beginning of the Sino-Western cultural communication, and later, western missionaries and sinologists translated it into various languages. The English translation of it started in the 18 century, and it was translated into various version during over 200 years. As an important part of international cultural exchange, the Book of Songs has forcefully pushed Chinese literature known to the world.

A Study on Body Narrative in New Media Art - Focusing on “Relative Space”

Hou Jing,Surng Gahb Jahng,Hyung-gi Kim 중앙대학교 영상콘텐츠융합연구소 2020 TechArt :Journal of Arts and Imaging Science Vol.7 No.4

Since ancient times, the body as a form of narrative has existed in a wide range of art forms. From ancient Greece to the second half of the 20th century, with the development of society, peoples understanding of the body has undergone a great change from the ternary theory of body and mind to the return of the body to its own nature. This paper researches body narration in new media art, and takes HOU JING’s work “Relative Space” as the main line. Furthermore, it uses the structure of thematic research to explore the basic artistic connotation conveyed by the body as a media tool.

정식 시기와 적심 횟수가 화단국화의 생장과 개화에 미치는 영향

姚婧超, 정해준, 김동찬, 이진희, 권민훈, 서병기 배재대학교 자연과학연구소 2013 自然科學論文集 Vol.24 No.1

This study was conducted to investigate optimum planting date and pinching times on the growth and flowering of garden chrysanthemum(Dendranthema grandiflorum Ramat.). 3 lines of garden mums were planted on May 16, June 13 and July 11 respectively. As planting date was delayed, all the 3 lines' plant height and plant width was reduced. 09-19-49 line got the biggest plant height and width, followed by 09-11-37 line. And 08-166-02 line was the smallest. In different planting times, the experiment group of May 16 and June 13 got the biggest plant height, plant width and stem diameter. However, in the experiment group of May 16, most of the plants got flattened. The experiment group of June 13 got the best ornamental value, and the ornamental value was reduced in the experiment group of July 11 by the short vegetative growth. 09-19-49 line got the most flowers, while in different planting time, the experiment group of May 16 got the most flowers. 09-11-37 line got the biggest flower diameter. 09-11-37 line and 09-09-51 line were planted in the containers on the rooftop. Each groups were pinched by 1, 2 and 3 times on July 2, July 26 and Aug 16 respectively. There was no effect on plant height, plant width, and stem diameter of 09-09-51 line and 09-11-37 line between 1-time-pinching group and 2-time-pinching group. But 3-time-pinching group was reduced. There was no significance in flower diameter of these two lines but the number of flowers was reduced by more pinching times. Therefore, in this experiment, the groups which were planted on June 13 got the best ornamental value and the recommended planting time is in the middle of June. Furthermore, 2 times of pinching was recommended.

Removal of Hg(II) from aquatic environments using activated carbon impregnated with humic acid

Jin, G.,Eom, Y.,Lee, T.G. Korean Society of Industrial and Engineering Chemi 2016 Journal of industrial and engineering chemistry Vol.42 No.-

The removal of Hg from aquatic environments using activated carbon (AC) impregnated with humic acid was investigated. The Hg adsorption capacity of the AC was controlled by the specific surface area and by surface functional groups, and the Hg adsorption process was exothermic and more effective at low pH. The Hg adsorption process in this study corresponds to a Lagergren pseudo-2nd-order model, indicating that it is controlled by the chemical rather than the physical properties of the AC. In addition, the equilibrium isotherm data fit the Langmuir isotherm better than the Freundlich model, indicating that the AC surfaces were uniform and that a Hg adsorbate monolayer formed at equilibrium.

대구시 전통축제 놀이의 활성화를 통한 노인건강 증진방안

이징연 대구보건대학 2002 대구보건대학 論文集 Vol.22 No.-

The purpose of this paper was to develop the activity of traditional festival for the senior citizens' health in Daegu city. Recently, the positive factors for a local festival has not been revealed because of the cultural extinction, and administrational expedient. These phases distorted the succession of traditional festival and their life. Furthermore, These phases also caused that these senior citizens lost their traditional origin. Daegu city has been regarded one of culturally important city in South Korea. Therefore, this study attempted to practice a political task that people respect and care old people for their health, entertainment and life in Daegu city. Finally, developing their health through providing traditional festival could be able to establish a relationship between senior people and society.

Harbart의 敎授本質論

崔定雄 대구효성가톨릭대학교 1985 연구논문집 Vol.30 No.1

The purpose of this study is to find governing principles of Herbart's methodology of instruction, that is, firstly Introduction, Secondly essence of Instruction, thirdly Steps of Instruction, fourthly development with its contribution and weakness in Herbartian method, finally conclusion. Johann Herbart(1776-1841) was a German Philosopher and a great educator known for his contributions first to formulate a science of education which based directly on ethics and psychology. He is therefore called the father of modern educational psychology and also the father of the modern science of education. From ethics he derived the end of instruction, from psychology, its method. Herbart was strongley under the influence of Lock, Lebniz, Kant, Fichte and Pestalozzi. He is usually represented as a disciple of pestalozzi and he is a Kantian. In the conflict of rationalism and empiricism of 18 century Herbart chose a middle position. He agreed with the rationalists that the soul is an entity, but he refused to accept their doctrine that Knowledge is innate. He agreed with the empirists that Knowledge arises from experience but he turned against the sensationalism of Locke and the materialixm of the french philosophers, especially illuminists. Herbart's pedagogy is that the only foundation of the whole education is instruction and educations as a science depends on a psychology. He is attempted to apply mathematics and physiological psy. to psychology and the aesthetic revelation of the world the supreme business of education, in a word, aesthetics to ethics. Knowledge comes from experience and presentations, further it is acquired by intercourse with others, especially from teachers in the work of instruction. Presentations result from the soul bringing about an inner reaction or creative process of recognition. Presentations are objects of thought which have bee presented, that is brought into consciousness. Knowledge is no longer goal, it is the only instrument to educational aims, and that knowledge is the basis of al the higher intellectual and moral development. The whole purpose of education may be summed up in the concept-marality or virtue. Virtue mean for Hebart five basic idears: ① the idear of inner freedom, ② the idea of perfection, ③ the idea of benevolence or good will, ④ the idea of right or Justice, ⑤ the idea of retrubution. Herbart's contribution to pedagogical theory is is doctrines of interest. For Herbart interest became the basic principle upon which depended all his other principles. Interest is an inner tendency making for the retention of an object of thought in consciousness. This interest comes from two laws of the mind: the law of frequency and the other the law of association. He found that the interests of man were many-sided, that they come from two main sources: man's contact with things and his relation with people. In the respective fields of interests consists of six: ① Knowledge or empirical ② sympathy ③ human ④ social ⑤ religious ⑥ aesthetic relations. Herbart's method have two principles, three process and four formal steps. The two principles are: the principle of concentration and correlation, analysis and synthetic. The three process of education are: ① Control (Regierung) which is controlled teachers of pupils behaviour, ② instruction (Unterricht) ③ discipline or Self-discipline (Zucht) He insisted that teaching procedure should be adapted to the stages discernible in the development of knowledge. He distinguished two pahses an intensive and an extensive. Vertiefung-aborption in or concentration on a subject, and Besinnung-coordination and systematisation of the results of Vertiefung. He recognize the four formal steps which are indispensable in every process of instruction, Usually termed the four steps of recitation these are: ① clearness ② association ③ system ④ method. The Herbartians have five steps: ① preparation ② presentation ③ association ④ generalization ⑤ application. Herbart's teaching method had great effect of modern education.

共沈현상을 이용한 폐수중의 微量 Cr^(+6)의 처리를 위한 연구

이징연 대구보건대학 1992 대구보건대학 論文集 Vol.13 No.-

This study is to investigate the removal efficiency of Cr^(+6) from waste water by using the coprecipitation phenomena. Experiments were performed mainly by using the jar tester at pH range of 8 to 11. Cr^(+6) concentration was 1㎎/ℓ with different concentrations of ammonium ferrous sulfate, which were 5 to 100㎎/ℓ as Fe^(+2). Following conclusion were obtained from the results of this study. Removal efficiency was highly improved compared to the conventional hydroxide precipitation treatment. The optimum conditions for Cr^(+6) removal is at pH range of 9 to 10 and over the ratio(㎎/ℓ Fe^(+2)㎎/ℓCr^(+6)) of 5. The added So₄^(-2) at pH range of 5 to 6 improved on the coprecipitation of Cr^(+6). The added Ca^(+2) at pH range 10 to 11 improved on the coprecipitation of Cr^(+6).