유전질환 등록 시스템 운영, 유전자 정보 Database 구축 및 유전자 진단 시스템 확립

구수경,이환석,손용규,김성수,이광수,김지인,서일선,진수정,정동기,오범석,정성철,이진성 국립보건연구원 2000 국립보건원보 Vol.37 No.-

Progress in the development of heating systems towards long pulse operation for KSTAR

Kwak, J.G.,Bae, Y.D.,Chang, D.H.,Chang, D.S.,Hong, B.G.,Hwang, C.K.,In, S.R.,Jeong, S.H.,Jin, J.T.,Jung, K.S.,Kim, B.R.,Kim, J.,Kim, S.K.,Kim, T.S.,Lee, D.W.,Lee, K.W.,Oh, B.H.,Seo, C.S.,Seo, M.S.,Yoo International Atomic Energy Agency 2007 Nuclear fusion Vol.47 No.5

<P>Construction of the Korea superconducting tokamak advanced research (KSTAR) tokamak is in its final phase. For the long-pulse KSTAR discharges, the ion cyclotron range of frequencies (ICRF) and neutral beam injection (NBI) heating systems are expected to play important roles through a selective heating of ions and electrons, control of the plasma pressure and current profiles, a core fuelling and beam diagnostics for the KSTAR. In addition, the ICRF system is expected to be used for possible discharge cleaning and assisting in the tokamak startup. In this paper, the recent progress in the development of the ICRF and the NBI heating systems is described. The four-strap ICRF antenna has been successfully tested for a voltage up to 41 kV for a pulse length of 300 s (to 46 kV for 20 s) in a test chamber. A prototype KSTAR NBI system has been developed. At present, the system has successfully produced a 1 MW beam power for 200 s and a 3.5 MW output beam power for 4 s.</P>

라온 극저온제어시스템 기반설비와 제어화면 구축 현황

윤성운(S. YOON),이상일(S. LEE),박미정(M. J. PARK),손창욱(C. W. SOHN),최용준(Y. CHOI),유정현(J. YOO),Lingxue JIN,김무상(M. KIM),김도균(D. KIM),기태경(T. KI),서미경(M. K. SEO),진민성(M. S. JIN) 대한기계학회 2022 대한기계학회 춘추학술대회 Vol.2022 No.11

RAON, a heavy ion superconducting linear accelerator has been installed in Daejeon, S. Korea. A cryogenic system, which was installed and tested its performance cool the accelerator down until 269 K. The system consists of a lot of components, cryogenic plants, a distribution box, cryogenic distribution lines, cryomodules and valve boxes. A cryogenic control system which is complicated and integrated by EPICS (Experimental Physics and Industrial Control System) is developed and the graphic user interface which is designed by CS-studio (Control System-studio) is developed. They were verified during the accelerator cool-down but they need some modification based on the cool-down operation. This paper summarizes the RAON cryogenic system infrastructure and graphic user interfaces.

라온 극저온제어시스템 기반설비와 제어화면 구축 현황

윤성운(S. YOON),이상일(S. LEE),박미정(M. J. PARK),손창욱(C. W. SOHN),최용준(Y. CHOI),유정현(J. YOO),Lingxue JIN,김무상(M. KIM),김도균(D. KIM),기태경(T. KI),서미경(M. K. SEO),진민성(M. S. JIN) 대한기계학회 2022 대한기계학회 춘추학술대회 Vol.2022 No.11

RAON, a heavy ion superconducting linear accelerator has been installed in Daejeon, S. Korea. A cryogenic system, which was installed and tested its performance cool the accelerator down until 269 K. The system consists of a lot of components, cryogenic plants, a distribution box, cryogenic distribution lines, cryomodules and valve boxes. A cryogenic control system which is complicated and integrated by EPICS (Experimental Physics and Industrial Control System) is developed and the graphic user interface which is designed by CS-studio (Control System-studio) is developed. They were verified during the accelerator cool-down but they need some modification based on the cool-down operation. This paper summarizes the RAON cryogenic system infrastructure and graphic user interfaces.

Metabolic engineering of Saccharomyces cerevisiae for production of spermidine under optimal culture conditions

Kim, S.K.,Jo, J.H.,Park, Y.C.,Jin, Y.S.,Seo, J.H. IPC Science and Technology Press 2017 Enzyme and microbial technology Vol.101 No.-

<P>Spermidine is a polyamine compound exhibiting important biological activities, such as increasing lifes-pan, inflammation reduction, and plant growth control. As such, many applications of spermidine as a bio-modulating agent are anticipated. However, sustainable and scalable production of spermidine has not been achieved yet. Therefore, construction of a spermidine production system using Saccharomyces cerevisiae was attempted in this study. In order to secrete spermidine into fermentation broth, TPO1 coding for the polyamine transporter was overexpressed in an engineered S. cerevisiae strain capable of accumulating high concentrations of spermidine. Through optimization of fermentation conditions, the resulting strain (OS123/pTPO1) produced 63.6 mg/l spermidine with a yield of 1.3 mg spermidine/g glucose. However, we observed that spermidine production was repressed in the presence of glucose. To circumvent this problem, the genetic modifications for overproducing spermidine were introduced into an engineered S. cerevisiae capable of fermenting xylose. In a fed-batch fermentation using a mixture of glucose and xylose, the resulting strain (SR8 OS123/pTPo1) produced 224 mg/l spermidine with a yield of 2.2 mg spermidine/g sugars. These results suggest that engineered yeast constructed in this study can be employed for the production of spermidine. (C) 2017 Elsevier Inc. All rights reserved.</P>

Pressure evolution of LiBaF₃:Eu²⁺ luminescence

Mahlik, S,Grinberg, M,Shi, Liang,Seo, Hyo Jin IOP Pub 2009 Journal of Physics, Condensed Matter Vol.21 No.23

<P>We investigated the single crystals of LiBaF3 doped with Eu2+ using high pressure spectroscopy, where high pressure was applied in a diamond anvil cell. Photoluminescence, time-resolved luminescence and luminescence kinetics at pressures from ambient to 200 kbar and at temperatures from 10 K to ambient were studied. At ambient conditions the luminescence spectra consisted of sharp lines peaked at 360 nm attributed to the P-6(7/2) --> S-8(7/2) transitions in the 4f(7) electronic configuration of Eu2+ (the zero-phonon line and five single-phonon repetitions) and a broad band extending between 375 and 475 nm attributed to Eu2+ trapped exciton recombination. When pressure was increased the Eu2+ trapped exciton emission disappears and was replaced by the sharper band peaked at 355 nm, attributed to the 4f(6)5d(1)(eg). 4f(7)(S-8(7/2)) transition in Eu2+. To analyze the pressure dependence of luminescence spectra a model of impurity trapped excitons was developed. At temperatures lower than 50 K only the sharp lines related to P-6(7/2) --> S-8(7/2) transitions were observed for all pressures considered. Analysis of low temperature spectra allowed us to estimate the energies of the fifth phonon modes and the values of the Gruneisen parameters.</P>

Nematicidal effect of Sparassis latifolia-derived sparassol and disodium sparassol against Bursaphelenchus xylophilus

Lee, S.K.,Lee, K.T.,Park, Y.B.,Jin, G.H.,Ka, K.H.,Seo, S.T. 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol. No.

<P>The pine wood nematode (Bursaphelenchus xylophilus) causes pine wilt disease, which is a major problem affecting pine forests. This study aimed to identify metabolites from Sparassis latifolia that have nematicidal activity and to investigate the effect of water-soluble formulations of the active compounds for use as trunk-injectable control agents against the nematode B. xylophilus. When the submerged cultures of S. latifolia were bioassayed against B. xylophilus, strain KFRI 747 showed the most potent activity (52.8%), followed by KFRI 1080 (44.4%) and KFRI 645 (34.8%); their activities were correlated with the concentration of sparassol in their mycelia. Six fractions (SLE1-6) were obtained from ethyl acetate extracts of the liquid culture medium; nematicidal activity was found only in SLE4 (which included sparassol). Sparassol bioassayed against B. xylophilus and had LC50 and LC95 of 84.92 and 132.13 ppm, respectively. Sparassol was modified to disodium sparassol using NaOH because sparassol was not water-soluble and thus was not useful as an agent for trunk injection. Disodium sparassol exhibited similar nematicidal activity to that found with sparassol. The trunk-injectable nematicides (abamectin and emamectin benzoate) were more effective than sparassol and disodium sparassol in terms of LC50 and LC95 values. However, sparassol has a more simple chemical structure than these compounds and could be more easily synthesized. To our knowledge, this is the first report indicating that disodium sparassol might be useful as nematicide for use against the pine wood nematode. (C) 2015 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.</P>

Enhanced ethanol fermentation by engineered Saccharomyces cerevisiae strains with high spermidine contents

Kim, S. K.,Jo, J. H.,Jin, Y. S.,Seo, J. H. Springer Science + Business Media 2017 Bioprocess and biosystems engineering Vol.40 No.5

<P>Construction of robust and efficient yeast strains is a prerequisite for commercializing a biofuel production process. We have demonstrated that high intracellular spermidine (SPD) contents in Saccharomyces cerevisiae can lead to improved tolerance against various fermentation inhibitors, including furan derivatives and acetic acid. In this study, we examined the potential applicability of the S. cerevisiae strains with high SPD contents under two cases of ethanol fermentation: glucose fermentation in repeated-batch fermentations and xylose fermentation in the presence of fermentation inhibitors. During the sixteen times of repeated-batch fermentations using glucose as a sole carbon source, the S. cerevisiae strains with high SPD contents maintained higher cell viability and ethanol productivities than a control strain with lower SPD contents. Specifically, at the sixteenth fermentation, the ethanol productivity of a S. cerevisiae strain with twofold higher SPD content was 31% higher than that of the control strain. When the SPD content was elevated in an engineered S. cerevisiae capable of fermenting xylose, the resulting S. cerevisiae strain exhibited much 40-50% higher ethanol productivities than the control strain during the fermentations of synthetic hydrolysate containing high concentrations of fermentation inhibitors. These results suggest that the strain engineering strategy to increase SPD content is broadly applicable for engineering yeast strains for robust and efficient production of ethanol.</P>

Lactoferrin causes IgA and IgG2b isotype switching through betaglycan binding and activation of canonical TGF-β signaling

Jang, Y-S,Seo, G-Y,Lee, J-M,Seo, H-Y,Han, H-J,Kim, S-J,Jin, B-R,Kim, H-J,Park, S-R,Rhee, K-J,Kim, W-S,Kim, P-H Society for Mucosal Immunology 2015 Mucosal immunology Vol.8 No.4

<P>Lactoferrin (LF), a pleiotropic iron-binding glycoprotein, is known to modulate the humoral immune response. However, its exact role in Ig synthesis has yet to be elucidated. In this study, we investigated the effect of LF on Ig production by mouse B cells and its underlying mechanisms. LF, like transforming growth factor (TGF)-beta 1, stimulated B cells to produce IgA and IgG2b, while downregulating other isotypes. Using limiting dilution analysis, LF was shown to increase the frequency of IgA-secreting B-cell clones. This was paralleled by an increase in Ig germ-line alpha (GL alpha) transcripts, indicating that LF plays a role as an IgA switch factor. Interestingly, LF directly interacted with betaglycan (TGF-beta receptor III, T beta RIII) and in turn induced phosphorylation of T beta RI and Smad3 through formation of the T beta RIII/T beta RII/T beta RI complex, leading to IgA isotype switching. Peroral administration of LF increased intestinal/serum IgA production as well as number of IgA plasma cells in lamina propria. Finally, we found that LF has an adjuvant activity when nontoxigenic Salmonella typhimurium was inoculated perorally, conferring protection against intragastrical infection of toxigenic S. typhimurium. These results suggest that LF has an important effect on the mucosal/systemic IgA response and can contribute to protection against intestinal pathogens.</P>

Molecular cloning and expression of fungal cellobiose transporters and β-glucosidases conferring efficient cellobiose fermentation in Saccharomyces cerevisiae

Bae, Y.H.,Kang, K.H.,Jin, Y.S.,Seo, J.H. Elsevier Science Publishers 2014 Journal of biotechnology Vol.169 No.-

Cellobiose was once regarded as a byproduct that should be removed from biomass hydrolysates because of its inhibitory activity to cellulases. It was revealed, however, that cellobiose could serve as a co-substrate for xylose fermentation by engineered Saccharomyces cerevisiae. Despite its advantages, to date, little is known about cellodextrin transporters that endow S. cerevisiae with cellobiose transporting ability. In this study, engineered S. cerevisiae strains capable of fermenting cellobiose were constructed by expressing various fungal cellobiose transporters and intracellular β-glucosidases. Among them, the strain expressing a putative sugar transporter from Penicillium chrysogenum (Pc_ST) and β-glucosidase from Thielavia terrestris (Tt_BG) showed an improved cellobiose fermentation performance compared to the strain expressing a cellodextrin transporter from Neurospora crassa (Nc_CDT-1) and β-glucosidase from N. crassa (Nc_GH1-1). Cellobiose fermentation by S. cerevisiae Pc_ST/Tt_BG under microaerobic conditions resulted in 14.5+/-0.5g/L of final ethanol concentration with a yield of 0.37+/-0.01g ethanol/g cellobiose, which are 22% and 26% higher than the corresponding values of S. cerevisiae Nc_CDT-1/Nc_GH1-1. These results suggest that the yield and rate of cellobiose fermentation can be improved by adopting optimal pairs of cellobiose transporters and β-glucosidase.