Effect of Light with Different Wavelengths on Nostoc flagelliforme Cells in Liquid Culture

( Yu Jie Dai ),( Jing Li ),( Shu Mei Wei ),( Nan Chen ),( Yu Peng Xiao ),( Zhi Lei Tan ),( Shi Ru Jia ),( Nan Nan Yuan ),( Ning Tan ),( Yi Jie Song ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.4

The effects of lights with different wavelengths on the growth and the yield of extracellular polysaccharides of Nostoc flagelliforme cells were investigated in a liquid cultivation. N. flagelliforme cells were cultured for 16 days in 500 ml conical flasks containing BG11 culture medium under 27 μmol·m-2·s-1 of light intensity and 25oC on a rotary shaker (140 rpm). The chlorophyll a, phycocyanin, allophycocyanin, and phycoerythrin contents in N. flagelliforme cells under the lights of different wavelengths were also measured. It was found that the cell biomass and the yield of polysaccharide changed with different wavelengths of light. The biomass and the yield of extracellular polysaccharides under the red or violet light were higher than those under other light colors. Chlorophyll a, phycocyanin, and allophycocyanin are the main pigments in N. flagelliforme cells. The results showed that N. flagelliforme, like other cyanobacteria, has the ability of adjusting the contents and relative ratio of its pigments with the light quality. As a conclusion, N. flagelliforme cells favor red and violet lights and perform the complementary chromatic adaptation ability to acclimate to the changes of the light quality in the environment.

Taguchi Approach for Anti-heat Stress Prescription Compatibility in Mice Spleen Lymphocytes In Vitro

Xiao-yu Zhu,Gui-lin Cheng,Feng-hua Liu,Jin Yu,Yu-jie Wang,Tong-quan Yu,Jian-qin Xu,Ming Wang 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.7

Heat stress (HS) may induce immunosuppression as well as inhibit the proliferation of lymphocytes. This study evaluated the effects on immune function of our prescription on splenic lymphocytes under HS as well as its compatibility. The effects of four herbal extracts from Agastache rugosa, Atractylodes lancea, Cortex Phellodendri, and Gypsum Fibrosum on heat treated splenic lymphocytes were investigated and the compatibility of the prescription was also explored by using the Taguchi method. This study revealed changes in proliferation by traditional Chinese medicines of splenic lymphocytes after HS. Proliferation in the HS group was significantly lower than the control group. Under HS, the effects of higher concentrations of Agastache rugosa (100 and 200 μg/mL), Atractylodes lancea (100 and 200 μg/mL), Cortex Phellodendri (50 and 100 μg/mL) and Gypsum Fibrosum (100 and 200 μg/mL) caused a significant increase on ConA/LPS-induced proliferation of lymphocytes than lower concentrations. We, therefore, conclude that the prescription of traditional Chinese medicines may recover splenic lymphocytes from the immunosuppression induced by HS. The Taguchi design, which allows rapid and high efficiency for the selection of the best conditions for our prescription on HS-treated splenic lymphocytes, demonstrated that Agastache rugosa (200 μg/mL), Atractylodes lancea (200 μg/mL), Cortex Phellodendri (100 μg/mL) and Gypsum Fibrosum (100 μg/mL)were the optimal conditions for the prescription. The validation experiment confirmed that our composition in optimum extraction conditions enhanced effects on ConA or LPS-stimulated lymphocytes under HS. The results showed that the Taguchi optimization approach is a suitable method for optimization of the composition of prescription.

Clinicopathological Features and Prognosis of Gastroenteropancreatic Neuroendocrine Tumors: Analysis from a Single-institution

Zeng, Yu-Jie,Liu, Lu,Wu, Heng,Lai, Wei,Cao, Jie-Zhi,Xu, He-Yang,Wang, Jie,Chu, Zhong-Hua Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Background: The gastroenteropancreatic neuroendocrine neoplasm (GEP-NEN) is the most common type of neuroendocrine neoplasm. We summarized data in our centre to investigate the clinicopathological features, diagnostic methods, therapeutic approaches and prognosis for this neoplasm to increase knowledge of this disease in Asian populations. Method: A total of 122 patients treated at Sun Yet-san Memorial Hospital of Sun Yat-sen University between January 2000 and December 2011 were analyzed retrospectively. Results: Pancreas was the most common site of involvement (65/122, 53.3%); this disease has no special symptoms; positive rates of chromogranin A (CgA) and synaptophysin (Syn) were 81.1% and 87.7%, respectively. The positive rate of Syn had statistical difference among the three grades, but not CgA. Some 68 patients had G1 tumors, 32 G2 tumors and 22 G3 tumors, and Chi-square test showed that higher grading was correlated with worse prognosis (${\chi}^2=32.825$, P=0.0001). A total of 32 patients presented with distant metastasis, and 8 cases emerged during following up. Cox proportional hazards regression modeling showed that the tumor grade (P=0.01), lymphatic metastasis (P=0.025) and distant metastasis (P=0.031) were predictors of unfavorable prognosis. The overall 5-year survival rate was 39.6%, the 5-year survival rate of G1 was 55.7%, and the G2 and G3 were 34.2% and 0%, respectively. Conclusions: The incidence of gastroenteropancreatic neuroendocrine tumors has risen over the last 12 years. All grades of these diseases metastasize readily, and further research regarding the treatment of patients after radical surgery is needed to prolong disease-free survival.

Manifestations of Internal Affliction and a Quest for Divine Salvation in John Donne’s Devotions upon Emergent Occasions III

Yu, Jie-ae 한국문학과종교학회 2018 문학과종교 Vol.23 No.3

본 논문은 존 던이 『드러나는 일들에 대한 묵상』 III에서 인간의 나약성, 죄, 그리고 한계성의 인식에서 비롯된 내적 분란을 어떻게 형상화하고 있는가를 보여주는데 있다. 또한 이러한 내적 혼돈과 불편함을 전지전능하신 신의 목적과 현시에 대한 명상을 통해 어떻게 저항하고 극복해 가는가를 분석한다. 존 던의 이 작품 여러 곳에서는 영적 불안정성, 인간 삶의 유한함, 인간사의 여러 한계에 대한 생각으로 인하여 비롯된 영적 불안함을 성스러운 신에 대한 통찰을 통해 대면하고 극복해 나가는 영적 순례를 보여주고 있다. 이 작품의 세 부분을 통하여 던은 구원에 대한 신성한 묵상을 통하여 내적으로 자기 파멸적인 고뇌를 해결하고자 노력하고 있음을 보여준다. This article aims to examine how John Donne’s Devotions upon Emergent Occasions III embodies his internal discomfort caused by persistent recognitions of human frailty, sinfulness, and limitation. It also investigates his resilient attempt to overcome such an internal predicament via contemplations into providential purpose and emancipation of the Almighty from physical and spiritual infirmities. Numerous textual evidences of the last prose indicate Donne’s spiritual pilgrimage into searching for divine insight to confront and overcome evil spirits, which have led to his inward turbulence charged with perceptions of instability, transitoriness, and boundary of manifold human occurrences. In three interlinking sections of the work, Donne conducts a vigorous investigation into inward distress, simultaneously undertaking a resilient quest for settling the persistent haunt of inner destructive power via his sacred awakenings of salvation.

Trend and Technology of Bigdata

YU-Jie Li,Seong-Yoon Shin,Hyun-Chang Lee,Sun-Kyoung Kang 한국정보통신학회 2017 2016 INTERNATIONAL CONFERENCE Vol.9 No.1

John Donne’s Devotions upon Emergent Occasions Ⅱ

Yu, Jie-Ae(유지애) 새한영어영문학회 2015 새한영어영문학 Vol.57 No.2

This article examines how John Donne reflects on the diverse aspects of vicissitudes in Devotions upon Emergent Occasions II. A number of textual passages enumerate the author’s contemplations into changeability of the world in general and his personal life in particular. Through the structure of “Meditation,” “Expostulation,” and “Prayer,” he investigates the thematic concerns with capriciousness, the ironic boundaries of human competence, and his spiritual enlivening into the perpetual presence and working of the Almighty, which remains beyond the mutable realm of human affairs and enterprises.

Promote Proline Accumulation in Apple Leaves By Bio-Regulator

Yu-Ling Jie,Hong-Qiang Yang,Wei Zhang,Hai-Zhou Zhao,Dei-Quan Li 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Shelley's Ideas of Reform in Prometheus Unbound

Yu, Jie-Ae 새한영어영문학회 2008 새한영어영문학 Vol.50 No.3

The purpose of this essay is to examine how Shelley deploys his thoughts on reform underlying his diverse portrayals of the classical figure Prometheus in Prometheus Unbound (1819). It argues that the characters of Prometheus embody Shelley's ideas of political, social, and moral reform in his lifetime. Incorporating the complexity of the historical conditions of his age, the poet constructs the symbolic ramifications of the protagonist, which extend into moral, political, and social aspects. He emphasizes the prerequisite requirement of moral reformation, which provides the basis for improving political and social establishments. The poet unfolds the notable reflections of the poem on the struggle for reformation which took place in his contemporary English society, while also considering the role of poetry in capturing that struggle.

Byron’s Marino Faliero : The Conflict between Willpowers in Venetian Society

Yu, Jie-Ae 한국중앙영어영문학회 2010 영어영문학연구 Vol.52 No.4

Little critical attention has been paid to Byron’s Marino Faliero, in particular, his evident concern with the feature and boundary of the protagonist’s unswerving willpower to pursue political intrigue against the ingrained corruption of Venetian oligarchy. This paper contends that a number of textual instances clearly exhibit the recurring motif such as the Doge’s assertive resolution for the rebellion which brings about its ironic, tragic consequences to both the protagonist and his conspirators. The main purpose of this article is to investigate how the poet depicts the irresolvable, conflicting relationship between Marino Faliero’s freedom of will and its externalization in the social organization regulated by Venetian aristocracy. The paper focuses on the procedure of the protagonist’s exercising of his willpower, which is intertwined with his own sense of fate and simultaneously the adverse, outer forces inherent within the arbitrary, corrupted power of the Council. Byron gradually unfolds the Doge’s vigorous pursuit of the freedom and glory of Venice, which features his rebellious attitude toward Venetian oligarchy. In portraying this, the author, at the same time, accentuates the questions of dilemma and limitation of man’s free choice, which struggles with the general will of the political organization. In addition, he elaborates other dramatic characters like Angiolina, Bertram and his defiant comrades, who play crucial role in propounding the complex, subtle aspects of the Doge’s willpower and activities.

John Donne`s Devotions upon Emergent Occasions I: Corporeal Infirmity, Misery, and Subsequent Reverberation

( Jie Ae Yu ) 한국현대영어영문학회 2015 현대영어영문학 Vol.59 No.1

This article aims to investigate how the first chapter of John Donne’s Devotions upon Emergent Occasions features his unswerving contemplations into his physical enervation and its vigorous influences on the inner workings of his mind. The author establishes one of the recurrent issues throughout the whole work ― his perception of wretchedness in the midst of the bodily ordeal. He depicts the present condition of discomfort, enquiring as to where man’s unforeseen, bitter occasion would possibly originate. While dealing with this subject, Donne simultaneously draws on the imperishable spirit inherent within his mind, which enables him to sustain the afflictive aftermaths of physical weakness. He elaborates on the coherent thematic conceptions by utilizing the intertwined structural patterns of “Meditation,” “Expostulation,” and “Prayer,” which are constituted within this chapter.(Changwon National University)