Silencing of Long Non-Coding RNA MALAT1 Promotes Apoptosis of Glioma Cells

Jianping Xiang,Shifeng Guo,Shuling Jiang,Yuelong Xu,Jiwei Li,Li Li,Jinyu Xiang 대한의학회 2016 Journal of Korean medical science Vol.31 No.5

The metastasis-associated lung adenocarcinoma transcription 1 (MALAT1) is a highly conserved long non-coding RNA (lncRNA) gene. However, little is known about the pathological role of lncRNA MALAT1 in glioma. In the present study, we explored the expression level of lncRNA MALAT1 in primary glioma tissues as well as in U87 and U251 glioma cell lines. Using qRT-PCR, we found that the expression of lncRNA MALAT1 was significantly increased in glioma tissues compared with that of paracancerous tissues. Meanwhile, the expression of MALAT1 was highly expressed in U98 and U251 cells. In order to explore the function of MALAT1, the expression of MALAT1 was greatly reduced in U87 and U251 cells transfected with siRNA specifically targeting MALAT1. Consequently, cell viability of U87 and U251 cells were drastically decreased after the knockdown of MALAT1. Concomitantly, the apoptosis rate of the two cell lines was dramatically increased. Furthermore, the expression levels of some tumor markers were reduced after the knockdown of MALAT1, such as CCND1 and MYC. In summary, the current study indicated a promoting role of MALAT1 in the development of glioma cell.

A Numerical Method of the Ship Structures Analysis Based on Meshless Local Petrov-Galerkin Method

Jianping Chen,Wen-yong Tang,Man-ping Xu 한국강구조학회 2015 International Journal of Steel Structures Vol.15 No.4

The paper presents a numerical simulation method for ship structures based on meshless local Petrov-Galerkin method. The proposal method is a suitable for adaptive computation method while processing the displacement and stress fields of the ship structures in high-gradient regions. Above all, based on the theory of Mindlin-Reissner plate, the field approximation function of the displacement is obtained by employing Moving-least Square method, and then the governing equation and the stiffness matrix of the structures are established. Numerical demonstrations show the solutions of the presented method are good agreement between FEM-ANSYS and the proposed approach, which verifies the validity of the presented method for the ship structures analysis.

A mesh-free analysis method of structural elements of engineering structures based on B-spline wavelet basis function

Jianping Chen,Wenyong Tang,Pengju Huang,Li Xu 국제구조공학회 2016 Structural Engineering and Mechanics, An Int'l Jou Vol.57 No.2

The paper is devoted to study a mesh-free analysis method of structural elements of engineering structures based on B-spline Wavelet Basis Function. First, by employing the moving-least square method and the weighted residual method to solve the structural displacement field, the control equations and the stiffness equations are obtained. And then constructs the displacement field of the structure by using the m-order B-spline wavelet basis function as a weight function. In the end, the paper selects the plane beam structure and the structure with opening hole to carry out numerical analysis of deformation and stress. The Finite Element Method calculation results are compared with the results of the method proposed, and the calculation results of the relative error norm is compared with Gauss weight function as weight function. Therefore, the clarification verified the validity and accuracy of the proposed method.

Co-expression of silkworm allatostatin-C receptor BNGR-A1 with its cognate G protein subunits enhances the GPCR display on the budding baculovirus

Jianping Chen,Jian Xu,Masato Hino,Mami Yamashita,Kazuma Hirata,Anandrao Ashok Patil,Tsuneyuki Tatsuke,Hiroaki Mon,Yutaka Banno,Takahiro Kusakabe,Jae Man Lee 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3

G protein-coupled receptors (GPCRs) are seven transmembrane proteins, which play an essential role in transmitting various extracellular signals into cells. For functional and structural analysis of GPCRs, it is necessary to produce active GPCRs in high quantitieswith outstanding purity. Fortunately, earlier baculovirus expression vector system has been reported as a proven functional GPCR mass-production tool. Therefore, in this study, we selected Bombyx mori allatostatin-C neuropeptide receptor BNGR-A1 as a GPCR reporter protein, which has been already proved successful binding of ligand. We confirmed its expression profile in various silkworm tissues and cell lines and then verified its plasma membrane subcellular localization in cultured silkworm BmN4 cells. In addition, we constructed recombinant baculoviruses for BNGR-A1, its ligand allatostatin-C (BmAST-C) and related eightG proteins (Gs, G12,Gα4, Gq, Gβ2,Gβ3, Gβ5 andGγ) and subsequentlymonitored the extracellular or intracellular expression of BNGR-A1 by co-infection with its ligand and cognate G proteins-expressing viruses. It is interesting to observe that different combinations of G proteins could result in changes or even undetectable of final yields of BNGR-A1, suggesting the essential roles of G proteins involved in the GPCR expression or stabilization. The present study demonstrated that co-infection of recombinant viruses expressing Gα4β3γ trimer enhanced the production of BNGR-A1 on BV fractions. To our knowledge, this is a fine strategy for identifying the specific G protein partner responsible for certain GPCR of interest. These studies would provide a novel idea for improving GPCR expression in the silkworm by baculovirus expression vector system.

Efficient Bioreduction of Ethyl 4-chloro-3-oxobutanoate to (S)-chloro-3-hydrobutanoate by Whole Cells of Candida magnoliae in Water/n-Butyl Acetate Two-phase System

Zhinan Xu,Limei Fang,Jianping Lin,Xiaoxia Jiang 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.1

The asymmetric biosynthesis of ethyl (S)-4-chloro-3-hydrobutanoate from ethyl 4-chloro-3-oxobutanoate was investigated by using whole cells of Candida magnoliae JX120-3 without the addition of glucose dehydrogenase or NADP+/NADPH. In a one-phase system, the bioconversion yield was seriously affected on the addition of 12.1 g/L ethyl 4-chloro-3-oxobutanoate. In order to reduce this substrate inhibition, a water/n-butyl acetate two-phase system was developed, and the bioreduction conditions optimized with regard to the yield and product enantiometric excess value. The optimal conditions were as following: water to n-butyl acetate volume ratio of 1:1, 4.0 g DCW/L active cells, 50 g/L glucose and 35°C. By adopting a dropwise substrate feeding strategy, high concentration of ethyl 4-chloro-3-oxobutanoate (60 g/L) could be asymmetrically reduced to ethyl (S)-4-chloro-3-hydrobutanoate with high yield (93.8%) and high enantiometric excess value (92.7%).

Efficacy of a DNA Vaccine Carrying Eimeria maxima Gam56 Antigen Gene against Coccidiosis in Chickens

Jinjun Xu,Yan Zhang,Jianping Tao 대한기생충학열대의학회 2013 The Korean Journal of Parasitology Vol.51 No.2

Tissue-associated profiling of gene expression in the fifth-instar larvae of Chinese Oak Silkworm, Antheraea pernyi

Duan Jianping,Liang Shimei,Zhu Zhenni,Yang Xinfeng,Li Ying,Xu Xin,Wang Jiazhen,Zhu Xuwei,Yao Lunguang 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

Antheraea pernyi is an edible insect with important economic value in both traditional and non-traditional ap plications. Elucidating the details of gene expression variation among different A. pernyi larval tissues will greatly improve our knowledge about A. pernyi biology and promote its protection and breeding. Here, tissue-based transcriptome and gene co-expression network analysis were performed for the fifth-instar larval tissues of testis, midgut, Malpighian tubule, head, trachea, silk gland, integument, hemocyte, ovary, and fat body. A total of 1,991 tissue-associated genes, including 62 transcription factor-encoding genes, were correlated with the development and functions of these tissues. Moreover, 10 gene co-expression modules that were highly corre lated with the tissues were also determined. The most important 30 genes with top connectivity in each module were identified as hub genes to form the core co-expression network with important biological functions in the corresponding tissues. Based on these resources, further in-depth studies should be conducted to determine the roles of candidate genes and transcription factors governing tissue development and trait formation in A. pernyi.

Modified nonlinear force density method for form-finding of membrane SAR antenna

Rui Xu,DongXu Li,Wang Liu,JianPing Jiang,YiHuan Liao,Jie Wang 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.54 No.6

Form-finding for cable-membrane structures is a delicate operation. During the last decades, the force density method (FDM) was considered to be an efficient method to address the problem. Many researchers were devoted to improving this method and proposed many methods such as natural force density method (NFDM), improved nonlinear force density method (INFDM), et al. In this paper, a modified nonlinear force density method (MNFDM) is proposed. In this method, the stresses of membrane elements were transformed to the force-densities of cable nets by an equivalent relationship, and then they can be used as initial conditions. By comparing with the forming finding results by using the FDM, NFDM, INFDM and MNFDM, it had demonstrated that the MNFDM presented in this paper is the most efficient and precise.

Dietary phosphorus deficiency impaired growth, intestinal digestion and absorption function of meat ducks

Huimin Xu,Shujun Dai,Keying Zhang,Xuemei Ding,Shiping Bai,Jianping Wang,Huanwei Peng,Qiufeng Zeng 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.12

Objective: An experiment was conducted to investigate the effects of dietary non-phytate phosphorus (nPP) deficiency on intestinal pH value, digestive enzyme activity, morphology, nutrient utilization, and gene expression of NaPi-IIb in meat ducks from 1 to 21 d of age. Methods: A total of 525 one-d-old Cherry Valley ducklings were fed diets (with 7 pens of 15 ducklings, or 105 total ducklings, on each diet) with five levels of nPP (0.22%, 0.34%, 0.40%, 0.46%, or 0.58%) for 21 d in a completely randomized design. Five experimental diets contained a constant calcium (Ca) content of approximately 0.9%. Body weight (BW), body weight gain (BWG), feed intake (FI), and feed to gain ratio (F:G) were measured at 14 and 21 d of age. Ducks were sampled for duodenum and jejunum digestion and absorption function on 14 and 21 d. Nutrient utilization was assessed using 25- to 27-d-old ducks. Results: The results showed ducks fed 0.22% nPP had lower (p<0.05) growth performance and nutrient utilization and higher (p<0.05) serum Ca content and alkaline phosphatase (ALP) activity. When dietary nPP levels were increased, BW (d 14 and 21), BWG and FI (all intervals), and the serum phosphorus (P) content linearly and quadratically increased (p<0.05); and the jejunal pH value (d 14), duodenal muscle layer thickness (d 14), excreta dry matter, crude protein, energy, Ca and total P utilization linearly increased (p<0.05); however, the serum ALP activity, jejunal Na+-K+-ATPase activity, and duodenal NaPi-IIb mRNA level (d 21) linearly decreased (p<0.05). Conclusion: The results indicated that ducks aged from 1 to 21 d fed diets with 0.22% nPP had poor growth performance related to poor intestinal digestion and absorption ability; but when fed diets with 0.40%, 0.46%, and 0.58% nPP, ducks presented a better growth performance, intestinal digestion and absorption function.

Fire Resistance Numerical Simulation of Extended End-Plate Steel Connection Joints with Different Strengthening Strategies under Fire

Jixiang Xu,Jianjun Wang,Jianping Han,Zhaolong Han,Jiahuang Tu,Zhanjie Li 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.4

The aim of this paper is to investigate the bolted end-plate beam to column (BEBC) joints with different strengthening strategies under fire through a parametric study by using a validated computational model. The current studies are mainly more focused on the mechanical behavior of a certain kind of reinforced or unreinforced BEBC joints at either room or fire alone, limiting investigations are available for a comprehensive stiffening strategy study of the BEBC joints under fire. Therefore, there is a need in improving the performance of BEBC joints with different stiffening strategies, the fire resistance comparison of these BEBC joints with different strengthening strategies under fire is of great interest. The analyses of the fire resistance behavior of the stiffened or unstiffened BEBC joints under fire were conducted by using a developed model that was established and validated by using the existing test results. A parametric study was conducted for BEBC joints with web stiffeners, end-plate stiffeners and web and end-plate stiffeners together under fire using the validated computational model. In order to better compare the fire resistance of BEBC joints with different strengthening strategies under a fire, 24 finite element models were established by considering different strengthening strategies and end-plate thicknesses. The results on different strengthening strategies and the end-plate thicknesses revealed the major impact of web stiffeners and end-plate web stiffener together, and the sensitivities of end-plate thickness for unreinforced BEBC joints. For the fire resistance of unreinforced and stiffened BEBC joints, the findings herein can provide a valuable reference for potential design recommendations.