McCoy 세포에서 Chlamydia trachomatis의 병원성 인자에 관한 연구

강정숙,최지영,차영주,김영주,김덕례,여명구,박열 조선대학교 생명과학연구소 1998 생명과학 연구 Vol.6 No.-

본 연구는 병원성 세균인 Chlamydia trachomatis(C. trachomatis)가 숙주세포에침습시 C. trachomatis의 병원성 인자와 C. trachomatis와 상호작용하는 숙주 세포막의 인자를 규명함으로써 숙주세포에 대한 감염율을 향상시켜 보다 향상된 진단 방법을 개발하기 위하여 수행하였다. C. trachomatis로 감염된 세포에서 기본소체의 막 단백질을 분리한 결과 각 혈청형의 39 kDa과 42 kDa에서 major outer membranes(MOMPs)이 관찰되었으며, 혈청형 E와 G의 MOMPs는 42.5 kDa으로 동일하였지만 혈청형 F, H, I, 그리고 K의 MOMPs는 각각 다르게 관찰되었다. C. trachomatis가 숙주세포로 침습시 관련된 병원성 인자에 대하여 조사하고자 단층세포로 배양된 배양용기에 C. trachomatis를 24, 48 그리고 73시간대별로 접종시켜 관찰한 결과 72시간의 MOMPs의 양이 가장 많이 증가하였으며 이 결과로 MOMPS이 C. trachomatis 병원성에 중요한 작용을 함을 확인할 수 있었다. C. trachomatis가 침습시 숙주세포의 막 단백질 변화양상을 관찰하기 위해 정상세포와 C. trachomatis로 1, 2, 3 그리고 4시간대별로 접종 시간을 달리한 숙주세포의 막 단백질을 분리한 결과 42 kDa의 단백질 양이 Chlamydia에 감염된 세포 내에서 증가됨을 관찰할 수 있었다. 이로 42 kDa의 막 단백질이 침습시 숙주세포 표면에 대한 수용체로서 작용함을 알 수 있었다. The present study was performed to analyze the pathogenic factors involved in the invasion process of Chlamydia trachomatis(C. trachomatis) into McCoy cells. In this study, elementary bodies(EBs) of C. trachomatis serotypes(E, F, G, H, I, K and LGV) could be isolated by the method of Percoll density gradient centrifugation and the pathogens of serotypes were also compared from whole-cell lysates by using sodium dodecyl sulfate-polyacrylamide gel elelectrophoresis. All chlamydial serotypes showed a single predominant protein ranging from 39 to 42 kDa. The major outer membrane proteins(MOMPs) of the E and G serotypes had an identical molecular weight of 42.5 kDa. In contrast, the MOMPs of the F, H, I and K serotypes were showed different molecular weights of MOMPs. By immunoblotting with anti-MOMP antibody it was revealed that the quantify of MOMPs was time-dependently increased when McCoy cells were infected with Chlamydia for 24, 48 and 72 hr. These results indicated that MOMPs are closely related to chlamydial infection into the host cell.

Microscopic and Spectroscopic Analyses of Pt-Decorated Carbon Nanowires Formed on Carbon Fiber Paper

Jeong, Namjo,Jang, Cheol-yong,Kim, Heeyeon,Jeong, Hakgeun,Yeo, Jeong-gu,Park, Yun Chang,Hwang, Kyo Sik Cambridge University Press 2013 Microscopy and microanalysis Vol.19 No.5

<B>Abstract</B><P>We report the synthesis of carbon nanowires (CNWs) via chemical vapor deposition using catalytic decomposition of ethanol on nanosized transition metals such as Co, Fe, and Ni. Dip-coating process was used for the formation of catalytic nanoparticles, inducing the growth of CNWs on the surface of the carbon fiber paper (CFP). The liquid ethanol used as carbon source was atomized by an ultrasonic atomizer and subsequently flowed into the reactor that was heated up to a synthesis temperature of 600-700°C. Microscopic images show that CNWs of <50 nm were densely synthesized on the surface of the CFP. Raman spectra reveal that a higher synthesis temperature leads to the growth of higher crystalline CNWs. In addition, we demonstrate the successful decoration of platinum nanoparticles on the surface of the prepared CNWs/CFP using the electrochemical deposition technique.</P>

Improvement of Mechanical Properties for Polymer-Impregnated Silica-Zircon Porous Composites

Jeong-gu Yeo,JeongSoo Park,SeungCheol Yang,Churl-Hee Cho 한국고분자학회 2016 한국고분자학회 학술대회 연구논문 초록집 Vol.41 No.2

체형인식 유형별 체중조절경험에 미치는 영향요인 비교

구여정(Yeo Jeong Gu),정재연(Jae Yeon Jeong),정지윤(Ji Yun Jeong),이해종(Hae Jong Lee) 한국보건교육건강증진학회 2019 보건교육건강증진학회지 Vol.36 No.4

Objectives: Perception types of body shape affects weight control. Appropriate understandings of actual body has a good effect on weight control, but misunderstanding of actual body condition is the opposite, which further has a negative impact on physical, mental health and so on. The purpose of this study is to examine the factor of affecting on weight control experiences by perception types of body shape. Methods: This study used data from the Korean Health and Nutrition Examination Surveys(KNHANES) Ⅵ, conducted in 2013-2015. Subjects were 8,509 aged more then 19 years, categorized as normal/normal perception, obesity/normal perception, normal/obesity perception, obesity/obesity perception according to perception types of body shape. Results: The factors affecting weight control experiences differ between perception types of body shape. For normal/normal perception type, affecting factors of weight control experiences were sex, age, education, marriage, physical activity and self-rated health. For obesity/normal perception type, affecting factors were education level, physical activity and self-rated health. For normal/obesity perception were only sex and self-rated health. For obesity/obesity perception type, affecting factors were sex, education level, drink, physical activity and self-rated health. Conclusions: The difference factors that influence weight control experiences by perception types of body shape suggest that there is a difference in motivation attempting to weight control by perception types of body shape. Therefore, it is necessary that the perception types of body shape should be considered when constructing an individual weight control plan or an obesity management education and prevention.

회동 수원지 물고기와 수영강 상류 저니토 중의 Polychlorinated Biphenyls 의 분포 상태

주여정(Yeo Jeong Joo),구미언(Mee On Gu),정종학(Jong Hak Jeong),정기호(Gi Ho Jeong) 한국환경분석학회 1998 환경분석과 독성보건 Vol.1 No.1

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Design and Implementation of an LED Mood Lighting System Using Personalized Color Sequence Generation

( Gu-min Jeong ),( Jong-yun Yeo ),( Dong Mook Won ),( Sung-han Bae ),( Kyung-joon Park ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.12

In this paper, we present a new LED (Light Emitting Diode) mood lighting system interacting with smartphones based on the generation of different light sequences. In the proposed system, one light sequence is considered to be one unit of the service contents, which is then transmitted through a network and played in an LED lighting system. To this end, we propose a novel generation scheme using a smartphone, and a decoding/playing mechanism in an LED lighting system. The lighting sequences have a fixed period divided into predefined time units. Two modes - basic and interpolation - are supported in each time unit when playing a color sequence. In the basic mode, the color is maintained for the entire time unit, whereas in the interpolation mode the color is interpolated. The sequence is decoded and played in the lighting circuit by changing the duty cycle of a PWM (Pulse Width Modulation) signal. A demonstration system of the overall proposed method was using smartphones, a server and an LED lighting system. The results from this experiment show the validity and applicability of the proposed scheme.

Ginsenoside fractions regulate the action of monocytes and their differentiation into dendritic cells

Yeo Jin Lee,Young Min Son,Min Jeong Gu,Ki-Duk Song,Sung-Moo Park,Hyo Jin Song,Jae Sung Kang,Jong Soo Woo,Jee Hyung Jung,Deok-Chun Yang,Seung Hyun Han,Cheol-Heui Yun 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.1

Background: Panax ginseng (i.e., ginseng) root is extensively used in traditional oriental medicine. It is a modern pharmaceutical reagent for preventing various human diseases such as cancer. Ginsenosidesd-the major active components of ginsengdexhibit immunomodulatory effects. However, the mechanism and function underlying such effects are not fully elucidated, especially in human monocytes and dendritic cells (DCs). Methods: We investigated the immunomodulatory effect of ginsenosides from Panax ginseng root on CD14⁺ monocytes purified from human adult peripheral blood mononuclear cells (PBMCs) and on their differentiation into DCs that affect CD4⁺ T cell activity. Results: After treatment with ginsenoside fractions, monocyte levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 increased through phosphorylation of extracellular signal-regulated kinase (ERK)1/2 and c-Jun N-terminal kinase (JNK), but not p38 mitogen-activated protein kinase (MAPK). After treatment with ginsenoside fractions, TNF-α production and phosphorylation of ERK1/2 and JNK decreased in lipopolysaccharide (LPS)-sensitized monocytes.We confirmed that DCs derived from CD14⁺ monocytes in the presence of ginsenoside fractions (Gin-DCs) contained decreased levels of the costimulatory molecules CD80 and CD86. The expression of these costimulatory molecules decreased in LPS-treated DCs exposed to ginsenoside fractions, compared to their expression in LPS-treated DCs in the absence of ginsenoside fractions. Furthermore, LPS-treated Gin-DCs could not induce proliferation and interferon gamma (IFN-γ) production by CD4⁺ T cells with the coculture of Gin-DCs with CD4⁺ T cells. Conclusion: These results suggest that ginsenoside fractions from the ginseng root suppress cytokine production and maturation of LPS-treated DCs and downregulate CD4⁺ T cells.

Patterns of failure in patients with locally advanced rectal cancer receiving pre-operative or post-operative chemoradiotherapy

Yeo, Seung-Gu,Kim, Min-Jeong,Kim, Dae Yong,Chang, Hee Jin,Kim, Min Ju,Baek, Ji Yeon,Kim, Sun Young,Kim, Tae Hyun,Park, Ji Won,Oh, Jae Hwan BioMed Central 2013 Radiation oncology Vol.8 No.-

<P><B>Background</B></P><P>We investigated patterns of failure in patients with locally advanced rectal cancer (LARC) according to chemoradiotherapy (CRT) timing: pre-operative <I>versus</I> post-operative. Also, patterns of failure, particularly distant metastasis (DM), were analyzed according to tumor location within the rectum.</P><P><B>Methods</B></P><P>In total, 872 patients with LARC who had undergone concurrent CRT and radical surgery between 2001 and 2007 were analyzed retrospectively. Concurrent CRT was administered pre-operatively (cT3–4) or post-operatively (pT3–4 or pN+) in 550 (63.1%) and 322 (36.9%) patients, respectively. Median follow-up period was 86 (range, 12–133) months for 673 living patients. Local recurrence (LR) was defined as any disease recurrence within the pelvis, and any failure outside the pelvis was classified as a DM. Only the first site of recurrence was scored.</P><P><B>Results</B></P><P>In total, 226 (25.9%) patients developed disease recurrence. In the pre-operative CRT group, the incidences of isolated LR, combined LR and DM, and isolated DM were 17, 21, and 89 patients, respectively. In the post-operative CRT group, these incidences were 8, 15, and 76 patients, respectively. LR within 2 years constituted 44.7% and 60.9% of all LRs in the pre-operative and post-operative CRT groups, respectively. Late (> 5 years) LR comprised 13.2% and 4.3% of all LRs in the pre-operative and post-operative CRT groups, respectively. The lung was the most common DM site (108/249, 43.4%). Lung or para-aortic lymph node metastasis developed more commonly from low-to-mid rectal tumors while liver metastasis developed more commonly from upper rectal tumors. Lung metastasis occurred later than liver metastasis (<I>n</I> = 54; 22.6 ± 15.6 <I>vs</I>. 17.4 ± 12.1 months; <I>P</I> = 0.035).</P><P><B>Conclusions</B></P><P>This study showed that LARC patients receiving pre-operative CRT tended to develop late LR more often than those receiving post-operative CRT. Further extended follow-up than is conventional may be necessary in LARC patients who are managed with optimized multimodal treatments, and the follow-up strategy may need to be individualized according to tumor location within the rectum.</P>

Selective synthesis and superconductivity of In–Sn intermetallic nanowires sheathed in carbon nanotubes

Jeong, Namjo,Yeo, Jeong-gu IOP Pub 2012 Nanotechnology Vol.23 No.28

<P>We demonstrate a simple and reproducible technique to synthesize crystalline and superconducting In–Sn intermetallic nanowires sheathed in carbon nanotubes (CNTs). The method is based on the catalytic reaction of C<SUB>2</SUB>H<SUB>2</SUB> over a mixture of both SnO<SUB>2</SUB> and In<SUB>2</SUB>O<SUB>3</SUB> particles. Importantly, tetragonal &bgr;-In<SUB>3</SUB>Sn and hexagonal &ggr;-InSn<SUB>4</SUB> nanowires with diameters of less than 100 nm are selectively synthesized at different SnO<SUB>2</SUB> to In<SUB>2</SUB>O<SUB>3</SUB> weight ratios. CNTs may serve as cylindrical nanocontainers for continuous growth of liquid-phased In<SUB>1−x</SUB>Sn<SUB>x</SUB> nanowires during growth process as well as for their solidification into In–Sn intermetallic nanowires during the cooling process. Microscopic and spectroscopic analyses clearly reveal evidence of a core–shell structure of the CNT-sheathed In–Sn intermetallic nanowires. Magnetization measurements show that the superconducting In–Sn nanowires have a critical magnetic field higher than the value of their bulk intermetallic compounds. Our method can be adopted to the nanofabrication of analogous binary and ternary alloys. </P>

Ginsenoside fractions regulate the action of monocytes and their differentiation into dendritic cells

Yeo Jin Lee,Young Min Son,Min Jeong Gu,Ki-Duk Song,Sung-Moo Park,Hyo Jin Song,Jae Sung Kang,Jong Soo Woo,Jee Hyung Jung,Deok-Chun Yang,Seung Hyun Han,Cheol-Heui Yun 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.3

Background: Panax ginseng (i.e., ginseng) root is extensively used in traditional oriental medicine. It is a modern pharmaceutical reagent for preventing various human diseases such as cancer. Ginsenosidesdthe major active components of ginsengdexhibit immunomodulatory effects. However, the mechanism and function underlying such effects are not fully elucidated, especially in human monocytes and dendritic cells (DCs). Methods: We investigated the immunomodulatory effect of ginsenosides from Panax ginseng root on CD14⁺ monocytes purified from human adult peripheral blood mononuclear cells (PBMCs) and on their differentiation into DCs that affect CD4⁺ T cell activity. Results: After treatment with ginsenoside fractions, monocyte levels of tumor necrosis factor (TNF)-a, interleukin (IL)-6, and IL-10 increased through phosphorylation of extracellular signal-regulated kinase (ERK)1/2 and c-Jun N-terminal kinase (JNK), but not p38 mitogen-activated protein kinase (MAPK). After treatment with ginsenoside fractions, TNF-α production and phosphorylation of ERK1/2 and JNK decreased in lipopolysaccharide (LPS)-sensitized monocytes.We confirmed that DCs derived from CD14⁺ monocytes in the presence of ginsenoside fractions (Gin-DCs) contained decreased levels of the costimulatory molecules CD80 and CD86. The expression of these costimulatory molecules decreased in LPS-treated DCs exposed to ginsenoside fractions, compared to their expression in LPS-treated DCs in the absence of ginsenoside fractions. Furthermore, LPS-treated Gin-DCs could not induce proliferation and interferon gamma (IFN-γ) production by CD4⁺ T cells with the coculture of Gin-DCs with CD4⁺ T cells. Conclusion: These results suggest that ginsenoside fractions from the ginseng root suppress cytokine production and maturation of LPS-treated DCs and downregulate CD4⁺ T cells.