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Insook Rhee Paeng 서울여자대학교 자연과학연구소 2003 자연과학연구논문집 Vol.15 No.-
High-performance resonance Raman/ absorption simultaneous determination technique for enzymatic reaction intermediate, compound Ⅱ, was performed upon addition of hydrogen peroxide to ferric Arthrornyces Ramosus Peroxidase (ARP) using microcirculating system. The Fe=O stretching, v(Fe=O), frequency of compound Ⅱ was observed at 788 cm^(-1) for the v(Fe=^(16)O) mode of ARP Ⅱ and at 750 cm^(-1) for the v (Fe=^(18)O) mode (△v = 35 cm^(-1)). The v(Fe=O) value found in compound Ⅱ of ARP is coincidently same as compound Ⅱ of HRP in alkaline pH. Also, it is shown that a trans ligand effect [v(Fe-N_(his))] is one of the important factors to influence to the v(Fe=O) frequency of compound Ⅱ.
Resonance Raman Study on Authrornyces Ramosus Peroxidase and its CO adducts
Insook Rhee Paeng 서울여자대학교 자연과학연구소 2003 자연과학연구논문집 Vol.15 No.-
We observed the v (FeCO) and v (CO) on ARPCO for the first time and δ (FeCO) was calculated using normal coordinate calculation under a bending angle of δ= 165°. The v (FeCO) and v (CO) on ARPCO was obtained at 519 cm^(-1) and 1928 cm^(-1) for the ^(12)C^(16)O derivative, and shifted to 516 cm^(-1) and 1884 cm^(-1) with ^(13)C^(16)O, and again to 510 cm^(-1) and 1885 cm^(-1) with ^(12)C^(18)O, and then to 506 cm^(-1) and 1840 cm^(-1) with ^(13)C^(18)O, respectively. Among various heme-CO, the v(CO) is the lowest value and v (FeCO) is the highest value. The CO-isotope-sensitive- band at 583 cm^(-1) was detected as seen for other heme protein-CO.
Mono-, Di-fluoro 5, 10, 15, 20-tetraphenylporphyrin Iron(Ⅲ)의 분광학적 연구
이인숙,신지영,김소영,김경아,김혜균 서울여자대학교 자연과학연구소 1997 자연과학연구논문집 Vol.8 No.-
Resonance Raman(RR) spectra of 5,10, 15, 20-tetraphenylporphyriniron(III) difluoride, [Fe(TPP)F_2], which was formed by the reaction between Fe(TPP)Cl and tetrabutylammonium fluoride(TBAF 3H_2O) in various non-aqueous solvents, were obtained with the 441.6nm(He-Cd laser) excitation. [Fe(TPP)F_2] is six-coordinate iron porphyrin with two polar Fe-F bonds. The porphyrin skeletal modes can be correlated with previously recorded mode of Ni(TPP) and [FeTPP]_2O. And these assignments were confirmed by observed wavenumber shifts due to ??Fe/^54Fe and TPP/TOO-d_8/TPP-N_15 isotopic substitutions. Difluoroiron complex is an iron(III) high-spin complex with the oxidation sensitive band at 1347cm^-1 for v_4 and core size/spin state sensitive band at 1541cm^-1 for v_2.
Fast mixing device를 이용한 Protein sample의 Time-Resolved Resonance Raman Spectroscopy
이인숙 서울여자대학교 자연과학연구소 2003 자연과학연구논문집 Vol.15 No.-
The application of fast mixing devices on the heme protein samples was described. Obviously, it is important to determine whether protein samples are stable under conditions encountered with new devices. Human Hemoglobin (Hb) solution was introduced into Platinum sphere mixing (PSM) system at 20 lb/in² gas pressure. The results were checked by UV-Vis spectroscopy and resonance Raman spectroscopy, and the spectra are superimposable upon those obtained from the initial Hb solution, respectively. The ligand binding reaction (oxygenation) of deoxy Hb was foIIowed by PSM. Time-resolved resonance Raman spectroscopy shows that the oxygenation reaction of Hb can be measured with PSM device for resonance Raman studies. Also, resonance Raman spectrum of product of the reaction between met Hb and HzOz supports that this Hb-H₂O₂ complex is ferryl Hb.
이인숙,최희선,김현숙 서울여자대학교 자연과학연구소 2004 자연과학연구논문집 Vol.16 No.-
A variety of labels have been used in the development of immunoassay. Compared to the other detection system, bioluminescence offers numerous advantages, such as high sensitivity, stability, and low detection limit. A sensitive competitive heterogeneous bioluminescence immunoassay for serotonin measurements was developed using a photoprotein, aequorin as a label. Avidin/biotin mediated assay was also performed for signal amplification and activity conservation of aequorin. Serotonin-avidin conjugate was prepared by Mannich reaction and biotin-aequorin conjugate was prepared through NHS-biotin. The resulting dose-response curve showed that the detection range for serotonin was 10^(-6) to 10^(-10)M, and the detection limit was 7.9 x 10^(-10)M. Cross reactivity test indicated that serotonin cross-reacted with 5-methoxytryptamine (28%) but not with 3-methylindole, melatonin, nor with 5-hydroxyindole-3-acetic acid.
Jeong, Sohee,Rhee Paeng, Insook The Scientific World JOURNAL 2012 The Scientific World Journal Vol.2012 No.-
<P>A competitive enzyme-linked aptamer assay (ELAA) to detect tetracycline in milk was performed by using two different aptamers individually; one is 76 mer-DNA aptamer and the other is 57 mer-RNA aptamer. The best optimum condition was obtained without monovalent ion, Na<SUP>+</SUP> and also by adding no Mg<SUP>2+</SUP> ion in the assay buffer, along with RT incubation. The optimized ELAA showed a good sensitivity (LOD of 2.10 × 10<SUP>−8</SUP> M) with a wide dynamic range (3.16 × 10<SUP>−8</SUP> M ~ 3.16 × 10<SUP>−4</SUP> M). In addition, the average R.S.D. across all data points of the curve was less than 2.5% with good recoveries (~101.8%) from the milk media. Thus, this method provides a good tool to monitor tetracycline in milk from MRLs' point of view. However, this ELAA method was not superior to the ELISA method in terms of specificity. This paper describes that it does not always give better sensitivity and specificity in assays even though aptamers have several advantages over antibodies and have been known to be good binders for binding assays.</P>