Effect of Reactor Type on the Purification Efficiency in the Fractional Precipitation Process

Insoo KANG,Jin-Hyun KIM 한국생물공학회 2011 한국생물공학회 학술대회 Vol.2011 No.10

Molecular Biological Analysis for the Identification of Meloidogyne Species on Strawberry in Korea

Heonil Kang,Namsook Park,Donggeun Kim,Insoo Choi 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04

This study is the identification of root-knot nematode (RKN), Meloidogyne hapla, from strawberry in Korea using molecular analyses. Strawberry plants showed localized stunting and galled roots. Molecular analyses of COⅡ/lrRNA, 28S rDNA D2-D3 segment and internal transcribed spacer (ITS) region were employed for the identification of Meloidogyne spp. Polymerase Chain Reaction (PCR) amplification of COⅡ/lrRNA region produced a single fragment ca 528bp. Restriction digestion of the amplified PCR products with Dra1 enzyme produced two fragments at 200 and 250bp indication M. hapla. 28S rDNA D2-D3 segment and ITS were cloned and sequenced. 28S rDNA D2-D3 segment and ITS region produced a single fragment of 1004bp and 560bp, respectively. In BLAST search in Genbank, all sequences accord with other known M. hapla. As a result, all of the RKN samples were M. hapla. This study suggests that the dominant species of RKN on strawberry is M. hapla in Korea.

교육법의 연구 대상과 과제

강인수(InSoo Kang) 한국교육행정학회 2007 敎育行政學硏究 Vol.25 No.3

이 연구의 목적은 교육법학의 핵심적 주제, 성격, 기본원리 및 연구대상을 고찰하고 한국의 교육법학의 과제를 제시하는 데에 있다. 연구내용을 정리하면 다음과 같다. 첫째, 교육법학의 핵심주제는 공교육체제에서 교육당사자간의 권리-의무관계라고 할 수 있다. 따라서 교육법학은 교육현상에서 제기되는 법률관계 즉 교육법현상을 교육학과 법학의 연구성과를 토대로 독자적 이론을 정립하면서 교육관계당사자의 법익을 조화롭게 규명하기 위한 학문 영역이라고 정의할 수 있다.둘째, 교육법학의 성격은 통합적, 독자적, 사회법적 성격을 지닌다고 할 수 있다. 셋째, 교육법학의 대상은 교육법의 이념과 기본원리 및 교육철학, 헌법을 포함한 교육관련 법령과 교육기관과 교육당사자의 교육활동 등이다. 교육법학의 목적은 국민의 교육을 받을 권리의 실현과 보장을 위한 이론을 구성하여, 교육당사자 상호간의 교육권의 충돌을 조정하며 교육입법과 교육재판에서 교육이론 및 공교육법리의 적용을 통하여 교육에서의 정의를 구현하기 위한것이다. 넷째, 교육법학의 과제는 교육관련 법령의 헌법 적합성을 제고하고, 교육당사자의 교육권 개념의 정립과 조화로운 보장, 교육입법 및 교육재판 과정에서의 교육의 특수성 반영, 교육학과 교육법학의 학제적 연구의 활성화, 교원과 국민의 교육법의식의 향상 그리고 한국 교육법학의 독립성 확립 등이라고 할 수 있다. The main purpose of this research is to identify the research areas of Educational Law and important issues for futher study. First, the main subject of the educational law is the right-duty relationship among the interested parties in the public education system. Educational law is to investigate the important legal issues which can be raised in the actual educational activity. Therefore, educational law is a science of originally theorizing about the various legal issues of the educational law based on the research products of both the education and the law, eventually pursuing to harmonize the legal rights of those who are engaged in the education activity. Second, the main characteristics of the educational law are its synthesis, originality and relationship with social law. Third, educational law has important purposes; to establish the theory regarding the realization and security of the right to education for all, to resolute the conflict among various interest groups over the educational rights, to realize the educational just through the application of the educational theory and public educational law theory. Fourth, the research areas of educational law are the fundamental and basic principle of educational law and educational activities and raws related educational administration and policy. Lastly, the important issues for futher study are to improve its consistency with the constitution, to establish and secure the right to education of all concerned parties, to examine its distinctiveness of legislation and legal judgement process, to increase the interdisciplinary research between education and education law, to raise the educational law-conscious of all, and to establish the independence of Korean educational law as a science.

Screening of Nematode Extraction Papers for Baermann Funnel Method

Heonil Kang,Jaehyun Lee,Insoo Choi,Donggeun Kim 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04

The Baermann funnel method requires three to four Kimwipes tissues for clean nematode extraction. To find more efficient and economical nematode extraction paper, 14 different kinds of tissue papers were tested and compared with Kimwipes tissues. Nematode species used in the extraction efficiency test were juvenile (J2) of Heterodera spp., J2 of Meloidogyne spp., Pratylenchus spp., Rhabditis spp., Acrobeloides spp., Panagrolaimus spp., Poikilolaimus spp. and Diplogasterida. Extraction efficiency varied between 42.0 to 88.8%. Considering costs, extraction efficacy, and cleanness, one sheet of Pulling Kitchen Towel (MONALISA Co., Korea) is selected.

동부품종의 국내 분포 뿌리혹선충 저항성 검정

강헌일 ( Heonil Kang ),전태환 ( Taehwan Jun ),권순욱 ( Soonwook Kwon ),김선태 ( Suntae Kim ),강항원 ( Hangwon Kang ),김용철 ( Yongchul Kim ),김동근 ( Donggeun Kim ),고형래 ( Hyoungrai Ko ),최인수 ( Insoo Choi ) 한국환경과학회 2018 한국환경과학회지 Vol.27 No.12

This study was conducted to evaluate resistance of 10 different cowpea cultivars which were introduced from the United States against two major root-knot nematodes, Meloidogyne arenaria and M. incognita, that affect greenhouse crop cultivation in Korea. The results showed that the resistance of the tested cultivars to the root-knot nematodes varied with the cultivar. Texas Cream 40 showed moderate resistance to M. arenaria whereas the other cultivars were susceptible. Purple Hull Pinkeye and Texas Pinkeye Purple Hull were found to be resistant to M. incognita, whereas Mississippi Silver showed moderate resistance and the other cultivars were susceptible. As the cultivars exhibit resistance to M. arenaria and M. incognita, such cultivars resistant to root-knot nematodes should be considered during the cultivation of cowpea as a green manure crop.

제올라이트 베타의 내재적 특성이 폴리올레핀의 저온 접촉유화에 미치는 영향

강종현(Jong Hun Kang),강한결(Hankyeul Kang),노인수(Insoo Ro),강기혁(Ki Hyuk Kang),정수화(Soohwa Jeong) 한국환경에너지공학회 2023 한국열환경공학회 학술대회지 Vol.2023 No.2

Pyrolysis is a well-established technology in the field of advanced recycling for polyolefin waste management. This process is pivotal in generating a spectrum of hydrocarbons, ranging from light naphtha to wax, thereby contributing to sustainable waste utilization practices. The integration of catalysts, particularly zeolites, presents an avenue to address the substantial energy consumption associated with conventional pyrolysis methods and to tailor the selectivity of product distribution. Notably, zeolite beta, characterized by its 12-membered-ring pore apertures measuring &-7 A in diameter, is well-known for its capability to yield a high liquid selectivity within the C5-C40 range during the catalytic cracking of polyolefins. The catalytic performances of zeolites in the context of polyolefin catalytic cracking are intricately associated with their intrinsic properties: Si/Al ratio, crystal dimensions, and mesoporosity. This work systematically delves into an exploration of the catalytic capabilities of various zeolite beta samples, wherein the Si/Al ratios, mesoporosity, and crystal sizes were controlled, specifically in the low-temperature (330 ° C) catalytic cracking of polyethylene. In a concerted effort to mitigate over-reaction and enhance conversion efficiency, an open batch reactor was employed.

DNA Methylation Regulates the Differential Expression of CX3CR1 on Human IL-7Rα^low and IL-7Rα^high Effector Memory CD8⁺ T Cells with Distinct Migratory Capacities to the Fractalkine

Shin, Min Sun,You, Sungyong,Kang, Youna,Lee, Naeun,Yoo, Seung-Ah,Park, Kieyoung,Kang, Ki Soo,Kim, Sang Hyun,Mohanty, Subhasis,Shaw, Albert C.,Montgomery, Ruth R.,Hwang, Daehee,Kang, Insoo American Association of Immunologists 2015 Journal of Immunology Vol. No.

<P>DNA methylation is an epigenetic mechanism that modulates gene expression in mammalian cells including T cells. Memory T cells are heterogeneous populations. Human effector memory (EM) CD8<SUP>+</SUP> T cells in peripheral blood contain two cell subsets with distinct traits that express low and high levels of the IL-7Rα. However, epigenetic mechanisms involved in defining such cellular traits are largely unknown. In this study, we use genome-wide DNA methylation and individual gene expression to show the possible role of DNA methylation in conferring distinct traits of chemotaxis and inflammatory responses in human IL-7Rα<SUP>low</SUP> and IL-7Rα<SUP>high</SUP> EM CD8<SUP>+</SUP> T cells. In particular, IL-7Rα<SUP>low</SUP> EM CD8<SUP>+</SUP> T cells had increased expression of CX3CR1 along with decreased DNA methylation in the <I>CX3CR1</I> gene promoter compared with IL-7Rα<SUP>high</SUP> EM CD8<SUP>+</SUP> T cells. Altering the DNA methylation status of the <I>CX3CR1</I> gene promoter changed its activity and gene expression. IL-7Rα<SUP>low</SUP> EM CD8<SUP>+</SUP> T cells had an increased migratory capacity to the CX3CR1 ligand fractalkine compared with IL-7Rα<SUP>high</SUP> EM CD8<SUP>+</SUP> T cells, suggesting an important biological outcome of the differential expression of CX3CR1. Moreover, IL-7Rα<SUP>low</SUP> EM CD8<SUP>+</SUP> T cells induced fractalkine expression on endothelial cells by producing IFN-γ and TNF-α, forming an autocrine amplification loop. Overall, our study shows the role of DNA methylation in generating unique cellular traits in human IL-7Rα<SUP>low</SUP> and IL-7Rα<SUP>high</SUP> EM CD8<SUP>+</SUP> T cells, including differential expression of CX3CR1, as well as potential biological implications of this differential expression.</P>

First report of five free-living nematode species(Nematoda: Rhabditida) from Korea

Kang, Heonil,Seo, Jongmin,Kim, Donggeun,Bae, Changhwan,Kim, Yongchul,Choi, Insoo The National Institute of Biological Resources 2019 Journal of species research Vol.8 No.3

Five free-living nematode species belonging to the order of Rhabditida are described for the first time in Korea. Two unrecorded species of Rhabditidae, Cruznema tripartitum (Linston, 1906) Zullini, 1982, and Poikilolaimus oxycerca de Man, 1895, and one species of Peloderidae, Pelodera strongyloides Schneider, 1860, were collected in Korea. C. tripartitum has nine pairs of genital papillae arranged in 2+2+1+4 differently from similar species of C. scarabaeum which has ten genital papillae arranged in 2+1+4+3. Poikilolaimus oxycerca has shorter tail (shorter than anal body diameter) compared to similar species of P. regenfussi which has longer tail(longer than anal body diameter). Pelodera strongyloides has shorter tail(1-1.5 times anal body diameters long) than P. punctata (4 anal body diameters long). Two unrecorded species of Panagrolaimidae, Panagrolaimus apicatus Schuurmans Stekhoven & Teunissen, 1938 and Panagrolaimus rigidus (Schneider, 1866) Thorne, 1937 were collected in Korea. P. apicatus has rounded and lower lip compared to similar species, P. margaretae Massey, 1964 which has finely pointed lip. P. rigidus has slender tail and lips rounded and flat barely separated when compared to similar species, P. subelongatus(Cobb, 1914) Thorne, 1937 which has plumper tail and lips are well separated.

Occurrence and Distribution of Root-Knot Nematodes in Kiwifruit Orchard

Kang Heonil,Je Hwanseok,Choi Insoo 한국식물병리학회 2023 식물병연구 Vol.29 No.1

The study was conducted to investigate the infestation and distribution of plant-parasitic nematodes on kiwi orchards in Korea. Plant parasitic nematodes genus and densities were investigated at a total of 102 sites in Jeollanam-do, Gyeongsangnam-do, and Jeju-do, which are the main production areas of domestic kiwi orchards. Plant parasitic nematodes detected were of 9 genera, including root-knot nematodes (Meloidogyne spp.), spiral nematodes (Helicotylenchus spp.), and needle nematodes (Paratylenchus spp.), and 56% of the 102 plantations were infected with root-knot nematodes. Root-knot nematodes were found to be the most important plant parasitic nematode in domestic kiwi orchards. The average density of root-knot nematodes is 97 per 300 cm³ soil, and there is concern about the kiwi yield reduction. As a result of identifying the root-knot nematode species: M. arenaria, M. hapla, M. incognita, and M. javanica. Among them, M. arenaria is the most dominant. As the plant parasitic nematode infection route in fruit trees is often spread through the transplantation of infected seedlings, attention should be paid to the production of nematode-free plants during the production and supply of kiwifruit plants.

Detoxification: A Novel Function of BRCA1 in Tumor Suppression?

Kang, Hyo Jin,Hong, Young Bin,Kim, Hee Jeong,Rodriguez, Olga C.,Nath, Raghu G.,Tilli, Elena M.,Albanese, Christopher,Chung, Fung-Lung,Kwon, Sang Hoon,Bae, Insoo Oxford University Press 2011 Toxicological sciences Vol.122 No.1

<P>Our studies found that BRCA1 levels negatively correlate with DNA adducts induced by Benzo(a)pyrene (BaP). Pulse-chase experiments showed that the increase in BaP-induced DNA adducts in BRCA1 knockdown cells may not be associated with BRCA1’s function in nucleotide excision repair activity; rather, it may be associated with its function in modulating transcriptional regulation. BRCA1 knockdown in MCF-10A cells significantly attenuated the induction of CYP1A1 following BaP treatment indicating that the increase in BaP-induced adducts in BRCA1 knockdown cells is not CYP1A1 dependent. However, our study shows that BRCA1 defective cells may still be able to biotransform BaP by regulating other CYP enzymes, including CYP1B1. Knockdown of BRCA1 also severely affected the expression levels of two types of uridine diphosphate glucorunyltransferase (UGT1A1 and UGT1A9) and NRF2. Both UGTs are known as BaP-specific detoxification enzymes, and NRF2 is a master regulator of antioxidant and detoxification genes. Thus, we concluded that the increased amount of BaP-induced DNA adducts in BRCA1 knockdown cells is strongly associated with its loss of functional detoxification. Chromatin immunoprecipitation assay revealed that BRCA1 is recruited to the promoter/enhancer sequences of UGT1A1, UGT1A9, and NRF2. Regulation of UGT1A1 and UGT1A9 expression showed that the induction of DNA adducts by BaP is directly affected by their expression levels. Finally, overexpression of UGTs, NRF2, or ARNT significantly decreased the amount of BaP-induced adducts in BRCA1-deficient cells. Overall, our results suggest that BRCA1 protects cells by reducing the amount of BaP-induced DNA adducts possibly via transcriptional activation of detoxification gene expression.</P>