Screening of Nematode Extraction Papers for Baermann Funnel Method

Heonil Kang,Jaehyun Lee,Insoo Choi,Donggeun Kim 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04

The Baermann funnel method requires three to four Kimwipes tissues for clean nematode extraction. To find more efficient and economical nematode extraction paper, 14 different kinds of tissue papers were tested and compared with Kimwipes tissues. Nematode species used in the extraction efficiency test were juvenile (J2) of Heterodera spp., J2 of Meloidogyne spp., Pratylenchus spp., Rhabditis spp., Acrobeloides spp., Panagrolaimus spp., Poikilolaimus spp. and Diplogasterida. Extraction efficiency varied between 42.0 to 88.8%. Considering costs, extraction efficacy, and cleanness, one sheet of Pulling Kitchen Towel (MONALISA Co., Korea) is selected.

An unrecorded species of Hemicycliophora litoralis (Criconematoidea: Hemicyliophorinae) from South Korea

Kang, Heonil,Eun, Geun,Ha, Jihye,Kim, Yongchul,Choi, Insoo,Kim, Donggeun The National Institute of Biological Resources 2016 Journal of species research Vol.5 No.3

Hemicycliophora litoralis is newly collected from Korea. Korean population is well matched and within a range of original description and additional record from Solomon island in de Man's of L, b, c, V, R, Rex, Ran, Roes, RV (ant), annule, excretory pore, stylet length, and tail length. Differences are observed in RV and RVan. RV and RVan of original description was 55 (48-69) and 14 (10-19) but Korean population is 68 (61-76) and 22 (19-24), respectively. H. litoralis described from Solomon island has RVan of 19.6 (17-23) and is similar to Korean population. H. litoralis from Solomon island has slight higher Rst value of 39 (35-59) compared to Korean population of 33.5 (29-38). The measurements of MB (%), s, G1, G2 values are newly given.

First report of five free-living nematode species(Nematoda: Rhabditida) from Korea

Kang, Heonil,Seo, Jongmin,Kim, Donggeun,Bae, Changhwan,Kim, Yongchul,Choi, Insoo The National Institute of Biological Resources 2019 Journal of species research Vol.8 No.3

Five free-living nematode species belonging to the order of Rhabditida are described for the first time in Korea. Two unrecorded species of Rhabditidae, Cruznema tripartitum (Linston, 1906) Zullini, 1982, and Poikilolaimus oxycerca de Man, 1895, and one species of Peloderidae, Pelodera strongyloides Schneider, 1860, were collected in Korea. C. tripartitum has nine pairs of genital papillae arranged in 2+2+1+4 differently from similar species of C. scarabaeum which has ten genital papillae arranged in 2+1+4+3. Poikilolaimus oxycerca has shorter tail (shorter than anal body diameter) compared to similar species of P. regenfussi which has longer tail(longer than anal body diameter). Pelodera strongyloides has shorter tail(1-1.5 times anal body diameters long) than P. punctata (4 anal body diameters long). Two unrecorded species of Panagrolaimidae, Panagrolaimus apicatus Schuurmans Stekhoven & Teunissen, 1938 and Panagrolaimus rigidus (Schneider, 1866) Thorne, 1937 were collected in Korea. P. apicatus has rounded and lower lip compared to similar species, P. margaretae Massey, 1964 which has finely pointed lip. P. rigidus has slender tail and lips rounded and flat barely separated when compared to similar species, P. subelongatus(Cobb, 1914) Thorne, 1937 which has plumper tail and lips are well separated.

First report of a bacteriovorus nematode, Diploscapter coronatus (Cobb, 1893) Cobb, 1913 (Rhabditida: Diploscapteridae), in Korea

Kang, Heonil,Eun, Geun,Ha, Jihye,Park, Namsook,Choi, Insoo,Kim, Donggeun The National Institute of Biological Resources 2017 Journal of species research Vol.6 No.2

Diploscapter coronatus (Cobb, 1893) Cobb, 1913 was first reported on oriental melon (Cucumis melo vars. makuwa Makino) roots from plastic film houses, Gyeongsangbuk-do, Andong, Korea (latitude $N36^{\circ}33^{\prime}$. longitude $E128^{\circ}29^{\prime}$). Diploscapter coronatus is most similar to D. pachys. Species in the genus Diploscapter have a visibly annulated cuticle. The stoma of D. coronatus is 1.5 times longer than the lip region width. The pharyngeal corpus is clearly separated from the isthmus and the vulva is situated around the mid-body. However, it differed by higher ratio of "a", filiform tail and had relatively blunt labial hooks. The Korean population is well matched and within the range of D. coronatus as described from Iran and Ethiopia in de Man's of L, b, c, c', and V. Female length, however, varied between populations: the Ethiopia population female length is longer than in the Korea population (396.4 vs 427.0) while females in the Iran population are smaller than in the Korea population (396.4 vs 350.0). Nematode size may vary due to environmental conditions such as food sources. The position of excretory pores in the Korean population were shorter 53.9-72.5 than in other populations (67-82 and 70-89). Males were uncommon. Males in the Korean population are smaller than females but larger(356.0) than males in the Indian population (306.0).

Occurrence and Distribution of Root-Knot Nematodes in Kiwifruit Orchard

Kang Heonil,Je Hwanseok,Choi Insoo 한국식물병리학회 2023 식물병연구 Vol.29 No.1

The study was conducted to investigate the infestation and distribution of plant-parasitic nematodes on kiwi orchards in Korea. Plant parasitic nematodes genus and densities were investigated at a total of 102 sites in Jeollanam-do, Gyeongsangnam-do, and Jeju-do, which are the main production areas of domestic kiwi orchards. Plant parasitic nematodes detected were of 9 genera, including root-knot nematodes (Meloidogyne spp.), spiral nematodes (Helicotylenchus spp.), and needle nematodes (Paratylenchus spp.), and 56% of the 102 plantations were infected with root-knot nematodes. Root-knot nematodes were found to be the most important plant parasitic nematode in domestic kiwi orchards. The average density of root-knot nematodes is 97 per 300 cm³ soil, and there is concern about the kiwi yield reduction. As a result of identifying the root-knot nematode species: M. arenaria, M. hapla, M. incognita, and M. javanica. Among them, M. arenaria is the most dominant. As the plant parasitic nematode infection route in fruit trees is often spread through the transplantation of infected seedlings, attention should be paid to the production of nematode-free plants during the production and supply of kiwifruit plants.

Responses of Heterodera sojae to HG type resistant soybeans

Heonil Kang,Jongmin Seo,Namsook Park,Eulsoo Yun,Donggeun Kim,Insoo Choi 한국농약과학회 2018 한국농약과학회 학술발표대회 논문집 Vol.2018 No.10

New soybean cyst nematode, Heterodera sojae

Heonil Kang,Donggeun Kim,Insoo Choi 한국농약과학회 2018 한국농약과학회 학술발표대회 논문집 Vol.2018 No.10

Comparison of body wall cuticle of cyst of H. glycines and H. sojae

Heonil Kang,Garam Han,Namsook Park,Eulsoo Yun,Donggeun Kim,Insoo Choi,Young Ho Kim 한국농약과학회 2018 한국농약과학회 학술발표대회 논문집 Vol.2018 No.10

Nematicidal Activities of Pesticides against Root-Lesion Nematode in Perilla frutescens

Heonil Kang,Hyoung-Rai Ko,Sekeun Park,Eunhyeong Park,Eunwha Kim,Byeong-Yong Park 한국농약과학회 2021 한국농약과학회 학술발표대회 논문집 Vol.2021 No.11

Perilla (Perilla frutescens (L.) Britton var. japonica), which is an annual herbaceous crop belonging to Lamiaceae family, is one of the most important crop in Korea. Its seeds have been used for cooking oil and seasoning dishes. And its fresh leaves have been directly consumed. Plant parasitic nematodes were detected in perilla cultivation fields in Korea, and the incidences of root-lesion nematodes (RLNs) were higher than other plant parasitic nematodes. Among the RLNs, Pratylenchus penetrans was widely distributed and causes chlorosis of the leaf, necrosis of the root and yield loss on perilla. This study was conducted to test the nematicidal activities to P. penetrans for control. Six pesticides, which are amisulbrom, cadusafos, imicyafos, fluazinam, fluopyram, and fosthiazate, showed activities of 27.5% to 100% in well plate. Four pesticides showed the higher nematicidal activity than other in vitro tested pesticides; 100% mortality of fluopyram, 97.7% of cadusafos, 84.6% of imicyafos and 83.2% of fosthiazate. In greenhouse pots, fluopyram, cadusafos, and imicyafos caused the greatest inhibition of reproduction of P. penetrans, with decreases of 100%, 98.8% and 98.6%, respectively. Interestingly, fluopyram treatment was indicated 100% nematicidal activity from in vitro and pot experiment. Thus, our results suggest that fluopyram could be used to control the P. penetrans in perilla cultivation fields.

Characterization of White Soybean Cyst Nematode, Heterodera sojae

Heonil Kang,Namsook Park,Eulsoo Yun,Donggeun Kim,Insoo Choi 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10

We reported a new white soybean cyst nematode, Heterodera sojae in 2016. This species is closely related to H. glycineswhich has the same host plant. Morphological observations of the cyst, female, male and second-stage juveniles indicatedthat this species is differed from those of H. glycines. Cysts of H. sojae appeared more round, shining and darker thanthat of H. glycines. The distribution of this new cyst nematode in soybean field in Korea is unknown yet. In 2016, 270soil samples were collected from soybean fields and examined the existence of H. glycines and H. sojae. Total of 111samples (41.1%) contained cysts. Among them 77% were H. glycines and 23% were H. sojae. Our results suggest thatextensive researches are required for white soybean cyst nematode.