Cuprizone affects hypothermia-induced neuroprotection and enhanced neuroblast differentiation in the gerbil hippocampus after ischemia

Kyu Ri Hahn,Woosuk Kim,Hyo Young Jung,Hyun Jung Kwon,Sung Min Nam,Tae Hyeong Kim,Jong Whi Kim,Dae Young Yoo,Dae Won Kim,Jung Hoon Choi,Yeo Sung Yoon,In Koo Hwang 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

In the present study, we investigated the effects of cuprizone on cell death, glial activation, and neuronal plasticity induced by hypothermia after ischemia in gerbils. Food was supplemented with cuprizone at 0.2% ad libitum for eight weeks. At six weeks after diet feeing, gerbils received transient forebrain ischemia with or without hypothermic preconditioning. Cuprizone treatment for 8 weeks increased the number of astrocytes, microglia, and pro-inflammatory cytokine levels in the hippocampus. In addition, cuprizone treatment significantly decreased the number of proliferating cells and neuroblasts in the dentate gyrus. Brain ischemia caused cell death, disruption of myelin basic proteins, and reactive gliosis in CA1. In addition, ischemia significantly increased pro-inflammatory cytokines and the number of proliferating cells and differentiating neuroblasts in the dentate gyrus. In contrast, hypothermic conditioning attenuated these changes in CA1 and the dentate gyrus. However, cuprizone treatment decreased cell survival induced by hypothermic preconditioning after ischemia and increased the number of reactive microglia and astrocytes in CA1 as well as of macrophages in the subcallosal zone. These changes occurred because the protective effect of hypothermia in ischemic damage was disrupted by cuprizone administration. Furthermore, cuprizone decreased ischemia-induced proliferating cells and neuroblasts in the dentate gyrus

경험적 항응고요법과 측정에 따른 개별적 항응고요법이 혈액투석시 항응고 및 투석에 미치는 효과

허영숙 ( Heo Yeong Sug ),장인선 ( Jang In Seon ),이명선 ( Lee Myeong Seon ),이유나 ( Lee Yu Na ),이형주 ( Lee Hyeong Ju ),송지숙 ( Song Ji Sug ),권정아 ( Kwon Jeong A ),이갑노 ( Lee Gab No ),신진호 ( Sin Jin Ho ),권영주 ( Kwon Yeo 대한신장학회 2004 Kidney Research and Clinical Practice Vol.23 No.2

목 적 : 혈액투석 중의 항응고요법은 체외순환회로에 혈액응고가 생기지 않으며 출혈을 일으키지 않을 만큼의 항응고 효과가 있어야 하지만, 대다수 투석실에서는 경험적으로 일정량의 헤파린을 균등하게 사용하여 과하거나 부족한 항응고 작용을 보이게 된다. 적절한 헤파린 투여를 위해서는, 그에 대한 개개인의 반응이 다양하므로 혈중 헤파린 농도를 측정하여 용량을 정하는 것이 필요하다. 이에 환자마다 필요한 헤파린 용량을 적용하는 항응고요법이 혈액투석 중의 항응고 및 투석에 미치는 효과를 파악하고자 하였다. 방 법 : 3개월 이상 유지혈액투석을 받고 있는 환자 24명을 대상으로 경험적으로 규정된 사용량인 헤파린 부하량과 유지량을 주고, 유지량은 투석 30분 후부터 종료 30분 전까지 지속적으로 주입하는 경험적 항응고요법 (empirical heparinization, EH)과 개개인의 activated clotting time (ACT) 추적으로 결정된 헤파린 부하량과 유지량을 주고, 유지량은 투석 개시부터 종료 1시간 전까지 지속적으로 주입하는 개별적 항응고요법 (individualized heparinization, IH)을 각각 임의 적용 후 교차 측정하였며, 2주 후 방법을 바꾸어 동일대상자 반복 측정 조사를 하였다. 결 과 : 분석된 대상자는 남자 8명, 여자 14명이였고, 당뇨 10명, 비당뇨 12명, 흡연자 3명, 비흡연자 19명이었다. 평균 연령 50.36±13.89세, 건체중 55.51±7.84㎏, 혈액투석 기간 54.48±66.09개월이었다. 투석 전후 헤모글로빈, 헤마토크리트, 혈소판수, 투석 전 알부민과 헤라핀 부하량은 두 방법이 차이가 없었으나, 헤파린 유지량은 개별적 항응고요법이 유의하게 많았다 (p=0.000). ACT의 적절한 연장과 유지 (p=0.000), 투석기 혈액 구획 용적 손실 (p=0.035), 혈액응고 (p=0.000)는 개별적 항응고요법이 항응고 효과가 좋았고, 출혈은 모두 없었다. 투석 전후, 다음 투석 전 요소질소와 투석 전 크레아티닌, 요소감소율과 투석적절도는 두 방법이 투석의 효과에 차이가 없었다. 결 론 : 이상의 결과로 ACT 측정에 따른 개별적 항응고요법이 경험적 항응고요법 보다 혈액 투석시 그에 따른 문제없이 투석의 효과를 유지하며 항응고 효과가 우월하였다. Background : Most chronic hemodialysis units select heparin doses on an empirical basis. Too little heparin causes clotting in the extracorporeal circuit and too much heparin may lead to excessive bleeding. We conducted a prospective, randomized, repeated cross over study to evaluate the effect of two different heparin regimens. The empirical standard dose regimen (empirical heparinization, EH) was used for all patients, and the individualized dose regimen (individualized heparinization, IH) determined by measuring the activated clotting time (ACT) was performed for more adequate heparinization during hemodialysis. Methods : Twenty-four outpatients with systemic heparinization who had been on hemodialysis for more than 3 months were enrolled. In both methods, anticoagulation was achieved with a loading dose and a continuous infusion of heparin. Each regimens were prescribed alternately, and repeated after 2 weeks later. The study evaluated pre-post dialytic Hgb, Hct, Platelet and predialytic albumin, heparin loading dose and infusion rate, ACT, total blood compartment volume (TBCV), visible blood clots, bleeding, pre-post dialytic and next predialytic BUN, predialytic Cr, URR, Kt/V_(urea). Results : Twenty-two patients were analyzed in this study. Pre-post dialytic Hgb, Hct, Platelet and predialytic albumin, heparin loading dose were not significantly different between two methods. But heparin infusion rate were significantly increased in individualized heparinization than in empirical heparinization. Activated clotting times were prolonged and maintained adequately in individualized heparinization during hemodialysis. The loss of TBCV and visible blood clots were significantly decreased in individualized heparinization than in empirical heparinization. There was no bleeding complication in two methods. Pre-post and next predialytic BUN, predialytic Cr, URR, Kt/V_(urea) were not significantly different between two methods. Conclusion : We concluded that the individualized heparinization can maintain adequate anticoagulation than the empirical heparinization without any other problems and compromising the delivery dose of dialysis. (Korean J Nephrol 2004;23(2):300-308)

Awareness and Use of Complementary and Alternative Medicine in Korean Lung Cancer Patients

( Joon Young Choi ),( Wonjun Ji ),( Chang-min Choi ),( Chaeuk Chung ),( Jae Myoung Noh ),( Cheol-kyu Park ),( In-jae Oh ),( Hong In Yoon ),( Hyeong Ryul Kim ),( Ho Young Kim ),( Chang Dong Yeo ),( Seu 대한결핵 및 호흡기학회 2021 Tuberculosis and Respiratory Diseases Vol.84 No.2

Background: Complementary and alternative medicine (CAM) has been used frequently, and its use continues to increase in lung cancer patients, despite insufficient scientific of its efficacy. To investigate this situation, we analyzed the current awareness and use of CAM in Korean lung-cancer patients. Methods: This prospective survey-based study was performed at seven medical centers in South Korea between August and October 2019. The survey assessed general patient characteristics and the awareness and use of CAM. We analyzed differences in the clinical parameters of patients aware and not aware of CAM and of CAM non-users and users. Results: Of the 434 patients included in this study, 68.8% responded that they were aware of CAM and 30.9% said they had experienced it. In univariate analysis, the patients aware of CAM were younger with poor performance status, had advanced-stage lung cancer, received more systemic therapy, and received concurrent chemoradiation therapy (CCRT). By multiple logistic regression, younger age, poor performance status, advanced stage, and prior CCRT were identified as independent risk factors for CAM awareness. There were no significant differences in the general characteristics and cancer-associated clinical parameters of CAM non-users and users. Conclusion: Specific clinical parameters were associated with patients’ awareness of CAM, although there were no significantly different characteristics between CAM users and non-users.

Survey of Metal Ions as Doping Agents in Lead Dioxide

Yeo, In Hyeong 東國大學校 1990 論文集 Vol.29 No.-

선택된 유기 및 무기 화합물의 산화를 위해 이산화납 전극의 전기화학적 활성을 변화시키기 위하여 도핑이온으로써 여러 가지 금속이온을 조사하였다.한 금속이온을 첨가한 수정된 이산화납 전극에서 선택 화합물의 산화에 대한 불균일 속도상수를 측정하였으며, 순수한 이산화납 전극에서 측정한 속도 상수와 비교하였다. 조사된 금속이온중에서 비스므스이온이 이산화납이 전기화학적 활성도를 가장 많이 증가 시켰으며, 반응에 참여한 전자수는 소모된 제어 전위 전기량법으로 결정하였다. Numerous metal ions as doping agents have been tried to change the electrocatalytic activity of lead dioxide electrode for the oxidation of selected organic and inorganic compounds. Heterogeneous rate constant for oxidation of a selected compound at modified lead dioxide with a metal ion was measured and compared to that at pure lead dioxide. Among the tested ions bismuth was the best for the increase of electrocatalytic activity of lead dioxide electrode. The number of electrons involved for several reactions were estimated by the exhaustive controlled potential coulometry.

Electrochemical Behavior of Nickel-base Alloy(Inconel 600) in Acidic Media : I.The Effect of Halide Ions on the Corrosion Rate 1. 부식속도에 대한 할로겐 이온의 효과

Yeo, In Hyeong,Yeon, Jei Won,Eom, Tae Yoon 東國大學校 1990 論文集 Vol.29 No.-

인코넬 600의 부식속도에 대한 할로겐 이온의 영향을 산성용액, 실온에서 측정한 편극곡선을 분석하여 연구하였다. 할로겐 이온이 포함된 1.0M 황산 및 0.1M 황산용액에서 얻은 양극 및 음극 편극곡선을 분석하여 부식전류, 부식전위, 및 Tafel 기울기를 구하였다. 주요음극 반응인 수소의 환원반응과 양극반응 모두 할로겐 이온에의해 영향을 받았다. 염화이온이 인코넬 600의 부식속도에 가장 크게 영향을 주었으며, 염화이온 농도에 따라 부식 속도가 증가하는 것이 관찰되었다. 브롬이온의 효과는 무시할 정도로 작았으며, 낮은 폐하용액에서 요오드이온의 존재는 부식속도를 증가 시키는 것으로 판명되었다. Effects of halide ions such as Cl_-, Br_-, and I_- on the corrosion rate of Inconel 600, high-nickel alloy, were studied from the polarization curves obtained in acidic media at room temperature. Corrosion current, corrosion potential and Tafel slope were determined from the analysis of anodic and cathodic polarization curves obtained in 0.1M and 1.0M H_2SO_4 containing the halide ions. It was concluded that the principal cathodic process, considered to be a hydronium discharge reaction, was partially screened and the anodic process was also influenced by the adsorption of the halide ions. Among the tested ions the chloride ion was determined to be the most corrosive chemical and the corrosion rate of Inconel 600 was linearly increased with the concentration of chloride. The effect of bromide ion on the corrosion rate of Inconel 600 was negligible, and the corrosion rate seemed to be increased in toe presence of iodide ion at low pH solutions.

Indirect Determination of Rare Earths by Amperometric Detection for Flow Injection Analysis

Yeo, In-Hyeong,Choi, Ke-Chon,Eom, Tae-Yoon Korean Chemical Society 1991 Bulletin of the Korean Chemical Society Vol.12 No.1

Comparative study of bilateral axillo-breast approach endoscopic and robotic thyroidectomy: propensity score matching analysis of large multi-institutional data

채영준,In Eui Bae,Hyun Soo Kim,Sang Gab Yoon,Jin Wook Yi,Hyeong Won Yu,Su-jin Kim,Young Jun Chai,Kyu Eun Lee,Yeo-Kyu Youn 대한외과학회 2020 Annals of Surgical Treatment and Research(ASRT) Vol.98 No.6

Purpose: The aim of this study was to compare the large multi-institutional data of surgical outcomes of bilateral axillo-breast approach (BABA) robotic (RT) and endoscopic thyroidectomy (ET) and to evaluate the merits of robotic thyroidectomy. Methods: From 2004 to 2015, 1,029 patients underwent BABA ET, and from 2008 to 2015, 2003 patients underwent BABA RT in 3 large volume centers in Korea. Two groups were retrospectively compared in terms of clinicopathologic characteristics, complications, surgical completeness, and long-term outcomes using propensity score matching analysis. Results: Both groups had similar demographic characteristics including age, sex, tumor size, pathologic stage, and hospital stay after matching. Each group had similar rate of transient hypoparathyroidism, however, ET showed significantly higher rate of permanent hypoparathyroidism (ET 5.2% vs . RT 2.3%, P = 0.05), and transient vocal cord palsy (ET 14.4% vs . RT 9.1%, P = 0.006). Total operation time was longer in the ET group irrespective of surgical extents, including lobectomy (P = 0.016), total thyroidectomy (P = 0.031), and total thyroidectomy with central lymph node dissection (P = 0.019). The rate of patients with off-Tg under 1.0 ng/mL after 1st ablation was significantly higher in RT than ET group (ET 64.6% vs . RT 92.7%, P < 0.001). In long-term follow-up of cancer patients, 1.4% experienced recurrence after ET (10 cases), while 0.3% cases experienced recurrence after RT (5 cases) (P < 0.001). Conclusion: Both ET and RT can be safe and effective methods to treat thyroid diseases. However, the application of robotic system may help to overcome the limitations of the instruments and surgeon’s skills

Increase in Discharge Capacity of Li Battery Assembled with Electrochemically Prepared V₂O₅/polypyrrole-composite-film Cathode

Kim, You-Na,Kim, Joo-Seong,Thieu, Minh-Triet,Dinh, Hung-Cuong,Yeo, In-Hyeong,Cho, Won-Il,Mho, Sun-Il Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.11

Flexible composite films of $V_2O_5$ and conductive polypyrrole ($V_2O_5$/PPy) were grown by facile electrochemical polymerization, wherein an anodization potential was applied to the substrate electrode in an electrolyte solution containing pyrrole monomer and dispersed $V_2O_5$ particles. The coating of polypyrrole (PPy) on the surface of $V_2O_5$ particles was induced by the oxidative catalytic action of $V_2O_5$ during the electrochemical polymerization of pyrrole. PPy in the composite film connects the isolated $V_2O_5$ particles. This results in the formation of conductive networks in the composite film cathode, thereby enhancing the Li+ ion diffusion to the surface of the isolated $V_2O_5$ particles and thus increasing the accessibility of the $Li^+$ ions. The specific capacity tests of the Li rechargeable batteries revealed that the discharge capacity of this composite film cathode was higher, i.e., $497\;mAhg^{-1}$, than that of $V_2O_5$/PPy powder or pristine $V_2O_5$.

철전극 표면 부동화막의 생성과 초기단계의 변화

여인형,백운기,In-Hyeong Yeo,Woon-Kie Paik 대한화학회 1984 대한화학회지 Vol.28 No.5

염기성 용액에서 기계적으로 연마한 고순도 철의 전위를 환원 전위로부터 부동화 전위로 급격히 변화시켜서 부동화 막이 전극표면에 형성되게 하면서 철의 반사율 변화와 타원편광반사법(Ellipsometry) 측정을 하였다. 철 표면이 부동화 될때 일어나는 반사광의 편광 파라메터(${\Delta},\;{\psi}$)와 반사율(R) 변화를 자동화된 타원편광반사계로 기록하였고, 이로부터 철을 부동화 상태에 들어가게 하는 표면막의 두께(${\tau}$)와 광학상수(n, k)들의 변화하는 값을 계산할 수 있었다. 광학상수 값들로 나타나는 막의 성질이 시간에 따라 급격한 전이를 하는 것은 관찰되지 않았으며, 비교적 짧은 시간(수초)내에 정상 상태 값에 접근하였다. 효과적으로 부동화를 일으키는 막의 두께는 $14\;{\sim}\;23{\AA}$의 범위에 있었다. 형성된 부동화 막은 용액의 pH가 큰 경우에는 얇고 치밀한 구조를 가진 것으로 보이며, pH가 작은 경우에는 두께는 두껍지만 pH가 큰 경우보다 덜 치밀한 부동화 막이 형성되는 것으로 보였다. 이들 부동화 막은 약간의 흡광성을 가지는 것으로 나타났다. Ellipsometric and reflectance measurements were made with magneto-optically self-nulling ellipsometer on the iron surface being passivated. The passivation was induced by abruptly changing potential of the mechanically polished high purity iron from the reduction potential to the oxidation potential in basic solutions. From the differences in the optical paramates(${\Delta},\;{\psi}$) and reflectance (R) between the reduced (film-free) and oxidized (film-covered) states, the thickness(${\tau}$) and optical constants (n, k) of the film in the early stage of its formation were computed as functions of pH and time. From the computed values, it was deduced that the properties of the anodic film did not undergo a drastic change with time which would indicate a transformation of the film before effective passivity is attained, and that the film reached its stady state within a few second. The thickness of anodic film was $14\;{\sim}\;23{\AA}$. The anodic films also seemed to have small values of optical absorption coefficient. The film formed in high pH environments had thinner and denser structure than that formed in low pH.

유산균을 이용한 흰목이 버섯의 발효를 통한 항비만과 항당뇨 효과

이준형(Jun-Hyeong Lee),김병혁(Byung-Hyuk Kim),윤여초(Yeo-Cho Yoon),김중규(Jung-Gyu Kim),박예은(Ye-Eun Park),박혜숙(Hye-Suk Park),황학수(Hak-Soo Hwang),권인숙(In-Sook Kwun),권기석(Gi-Seok Kwon),이중복(Jung-Bok Lee) 한국생명과학회 2019 생명과학회지 Vol.29 No.4

흰목이 버섯(Tremella fuciformis Berk)은 고혈압, 당뇨병, 비만, 폐질환, 미용에 효과가 있으며, 고대 중국에서는 불로장생약이라고 알려져 있다. 본 연구에서는 생물전환공정을 통해 발효된 흰목이 버섯 추출물의 항비만 및 당뇨병 효과를 조사하였다. 우리는 비만 유도 시킨 C57BL / 6N 마우스에 High Fats Diet (HFD), 5% non-fermented Tremella fuciformis (TF), 2.5% fermented Tremilla fuciformis (FTF), 5% FTF를 8주간 투여하였다. 경구당 부하검사는 7주간 식이섭취 후 실시하였으며, 8주간 식이섭취 후 섭취량, 식이섭취효율, 체중, 간, 부고환 지방량 및 혈청인슐린 수치 및 HOMA-IR을 분석하였다. 또한, 혈청 총콜레스테롤 함량 및 중성지방 함량을 분석하였다. FTF (실험)군과 TF (실험)군 및 HFD (실험)군과 비교한 결과 5% FTF (실험)군이 체중, 조직 중량, 중성지방 함량 및 HOMA-IR을 감소시키는 것을 확인하였다. 이 결과를 통해 발효 흰목이 버섯은 항비만 효과를 확인하였다. 마지막으로, 본 연구는 발효 흰목이 버섯을 이용한 비만 치료의 기초자료로 사용하고자 한다. White jelly fungus (Tremella fuciformis Berk) is effective for hypertension, diabetes, obesity, lung disease, beauty, and has been known as an elixir of life in ancient Chinese dynasty. In this study, we investigated the anti-obesity and diabetic effects of the fermented white jelly fungus extract by the bioconversion process. We fed an obesity-inducing mouse with 5% non-fermented Tremella fuciformis (TF), 2.5% fermented Tremella fuciformis (FTF), and 5% FTF containing High Fats Diet (HFD) and HFD for 8 weeks, respectively. The oral glucose tolerance test (OGTT) was performed analysis after 7 weeks of feeding and the dietary intake, food efficiency ratio, body weight, liver, epididymal fat weight, and serum insulin level were measured after 8 weeks of feeding. Also, HOMA-IR was analyzed. The concentrations of serum total cholesterol, triglycerides test was analyzed. The FTF compare with 5% TF and HFD confirmed that 5% FTF reduced body weight, tissue weight, triglycerides concentration, HOMA-IR, respectively. As a result, we confirmed that the fermented white jelly fungus has the antiobesity effect. Finally, this study can be used a basic data for obesity treatment using fermented white jelly fungus.