소화성 궤양 환자의 Helicobacter pylori 제균 요법에서 Probiotics의 역할

전혜진(Hyejin Chun),이상화(Sang Wha Lee),이홍수(Hong Soo Lee),심경원(Kyung Won Shim) 대한임상노인의학회 2012 대한임상노인의학회지 Vol.13 No.2

Background: Recent studies indicate a potential role of probiotics in the improvement of Helicobacter pylori (H. pylori ) eradication rate and in the prevention of H. pylori treatment-related side effects. We examined the effect of probiotics composed of Bacillus subtilis and Streptococcus faecium on the H. pylori eradication. Methods: A total 217 H. pylori positive patients were randomly assigned into two groups: 115 patients (group A) underwent the “triple therapy” (omeprazole 20 mg, amoxicillin 1 g and clarithromycin 500 mg b.i.d. for 7 days), while 102 patients (group B) underwent a modified eradication therapy (“triple therapy”+probiotics 1 capsule b.i.d. for 7 days). The eradication rate of H. pylori was evaluated after 4 weeks of completion of the treatment. Side effect profile, compliance and tolerability were assessed by a validated symptom questionnaire. Results: Intention-to-treatment analysis showed 71.3% (82/115) patients from the group A and 79.4% (81/102) patients from the group B have been successfully eradicated (P=0.04). H. pylori eradication rate according to per-protocol analysis, although higher in the group B, was statistically similar: 80.6% (79/99) versus 88.9% (80/90), respectively (P=0.11). Nausea, bloating and diarrhea were more commonly found in the group A than group B (P<0.01). The other side effects were similar in both groups. In the overall judgement of compliance and tolerability, there were no significant differences between two groups. Conclusion: Supplementation with probiotics could improve the eradication of H. pylori infection and decrease H. pylori eradication-associated nausea, bloating and diarrhea. 연구배경: Bacillus subtilis와 Streptococcus faecium 균주로 구성된 probiotics을 H. pylori (H. pylori) 제균 치료에 추가했을 때 제균율 향상 및 부작용, 환자 순응도, 치료 내성의 개선 효과에 대해 알아보고자 한다. 방법: 상부위장관 내시경에서 H. pylori 감염이 확인된 소화성 궤양 환자들을 omeprazole 20 mg, amoxicillin 1 g, clarithromycin 500 mg을 하루 2회 일주일간 복용하는 군(A군)과 삼제요법에 probiotics 1 capsule을 하루 2회 일주일간 함께 복용하는 군(B군)으로 무작위 배정하여 치료 후 H. pylori 제균율 및 부작용, 환자 순응도, 치료 내성에 대해 비교 분석하였다. 결과: 전체 217명 환자의 H. pylori 제균율(Intention-to-treatment)은 75.1%(A군 71.3%, B군 79.4%)로 probiotics을 투여한 군에서 제균율이 더 높았다(P=0.04). 연구를 완료한 198명 중 비순응 환자 9명을 제외한 189명의 H. pylori 제균율(Per-protocol)은 84.6%였으나 두 군 사이에 유의한 차이는 없었다(P=0.11). 오심, 고창, 설사 등 부작용이 B군에서 유의하게 낮았다. 결론: 기존의 H. pylori 제균에 보조요법으로서 probiotics의 처방은 기존의 삼제요법보다 H. pylori 제균율상승 및 오심, 고창, 설사 등 부작용 감소에 효과가 있다.

Structural characterization of flavonoid glycosides in Jeju Putgyul (Unripe citrus) extract using liquid chromatography coupled with electrospray ionization ion trap mass spectrometry

Hyejin Hyeon,Boram Go,Ho Bong Hyun,Sung Chun Kim,Seon-A Yon,Dae-Ju Oh,Weon-Jong Yon,Yong-Hwan Jung,Young-Min Ham 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

Protective Effect of Processed Panax ginseng, Sun Ginseng on UVB-irradiated Human Skin Keratinocyte and Human Dermal Fibroblast

Hyejin Lee,Joo Yeop Lee,Kyu Choon Song,Jinhee Kim,Jeong Hill Park,Kwang-Hoon Chun,Gwi Seo Hwang 고려인삼학회 2012 Journal of Ginseng Research Vol.36 No.1

In this study, we investigated the protective effects of processed Panax ginseng, sun ginseng (SG) against the UVB-irradiation on epidermal keratinocytes and dermal fi broblasts. Pretreatment of SG in HaCaT keratinocytes and human dermal fi broblasts reduced UVB-induced cell damage as seen by reduced lactate dehydrogenase release. We also found that SG restored the UVB-induced decrease in anti-apoptotic gene expression (bcl-2 and bcl-xL) in these cells, indicating that SG has an anti-apoptotic effect and thus can protect cells from cell death caused by strong UVB radiation. In addition, SG inhibited the excessive expression of c-jun and c-fos gene by the UVB in HeCaT cells and human dermal fi broblasts. We also demonstrated that SG may exert an anti-infl ammatory activity by reducing the nitric oxide production and inducible nitric oxide synthase mRNA synthesis in HaCaT keratinocytes and human dermal fi broblasts. This was further supported by its inhibitory effects on the elevated cyclooxygenase-2 and tumor necrosis factor-α transcription which was induced by UVB-irradiation in HaCaT cells. In addition, SG may have anti-aging property in terms of induction of procollagen gene expression and inhibition of the matrix metalloprotease-1 gene expression caused by UVB-exposure. These fi ndings suggest that SG can be a potential agent that may protect against the dermal cell damage caused by UVB.

Characterization of Essential Oils and Non-volatile Metabolites in Flower Buds between Magnolia denudata Desr. and Magnolia liliiflora Desr

Hyejin Hyeon,Boram Go,Ho Bong Hyun,Sung Chun Kim,Seon-A Yoon,So Yeon Oh,Yong-Hwan Jung,Young-Min Ham 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

In this study, the essential oil (EO) and the major non-volatile metabolites in white flower buds of Magnolia denudata and violet flower buds of Magnolia liliiflora were analyzed using GC-MS and HPLC. The dominant EO compounds found in the flower buds were monoterpene hydrocarbons (MO), e.g. sabinene (15.8-14.6%), β-myrcene (17.2-9.9%), and β-pinene (12.9-7.7%). Univariate and multivariate analysis were subsequently performed to reveal the relationships between EO compounds and non-volatile metabolites in white and violet flower buds. The results revealed that most of monoterpenes and phenolic acids in the white flower buds, such as sabinene, β-pinene, and rutin, were found to be significantly higher compared to violet flower buds. Furthermore, hierarchical clustering analysis of correlation coefficients suggested a positive correlation between MO and phenolic compounds. Our findings revealed that the metabolic patterns of both EO and non-volatile compounds could reflect the metabolic characteristics of the different colored flower buds. Further studies will be conducted to investigate the biological activities derived from EO and non-volatile compounds.

The new obesity-associated protein, neuronal growth regulator 1 (NEGR1), is implicated in Niemann-Pick disease Type C (NPC2)-mediated cholesterol trafficking

Kim, Hyejin,Chun, Younghwa,Che, Lihua,Kim, Jeongbeom,Lee, Sungjoong,Lee, Soojin Elsevier 2017 Biochemical and biophysical research communication Vol. No.

<P><B>Abstract</B></P> <P>Neuronal growth regulator 1 (NEGR1) is a newly identified raft-associated protein, which has recently been spotlighted as a new locus related to human obesity. Niemann-Pick disease Type C2 (NPC2) protein functions as a key player in the intracellular cholesterol trafficking, and its defect is linked to a fatal human neurodegenerative disease, NPC. In this study, we identified that NEGR1 interacts with NPC2 and increases its protein stability. Ectopically expressed NEGR1 proteins relieved an abnormal cholesterol accumulation in endosomal compartments. Importantly, NEGR1-defective mouse embryonic fibroblast cells exhibit increased cholesterol levels and triglyceride contents. These findings provide the first insight into the role of NEGR1 in intracellular cholesterol homeostasis, possibly explaining the missing link between NEGR1 with human obesity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> NEGR1 interacts with NPC2, a key player in intracellular cholesterol trafficking. </LI> <LI> NEGR1 co-localizes with NPC2 in the late endosomes and increases NPC2 stability. </LI> <LI> NEGR1 overexpression relieved cholesterol accumulation in endosomal compartments. </LI> <LI> These findings provide the first insight into the role of NEGR1 in intracellular cholesterol homeostasis and human obesity. </LI> </UL> </P>

장애인 구인구직 미스매치 해소를 위한 디지털 기반 강화방안

전희주(Chun, Heuiju),김용희(Kim, Yonghee),강미희(Kang, Mihee),권혜진(Kwon, Hyejin),김민경(Kim, Minkyeong),김민영(Kim, Minyeong) 한국장애인고용공단 고용개발원 2021 위탁과제보고서 Vol.2021 No.-

직류배전 시스템의 다중 에너지 저장장치 시스템을 위한 적응 드룹 제어

김혜진(Hyejin Kim),전창윤(Chang Yoon Chun),서갑수(Gab-Su Seo),이경준(Kyoungjun Lee),조보형(Bo-Hyung Cho) 전력전자학회 2014 전력전자학술대회 논문집 Vol.2014 No.7

Protective effect of Korean Red Ginseng against glucocorticoid-induced osteoporosis in vitro and in vivo

Jinhee Kim,Hyejin Lee,Ki Sung Kang,Kwang-Hoon Chun,Gwi Seo Hwang 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.3

Background: Glucocorticoids (GCs) are commonly used in many chemotherapeutic protocols and play an important role in the normal regulation of bone remodeling. However, the prolonged use of GCs results in osteoporosis, which is partially due to apoptosis of osteoblasts and osteocytes. In this study, effects of Korean Red Ginseng (KRG) on GC-treated murine osteoblastic MC3T3-E1 cells and a GC-induced osteoporosis mouse model were investigated. Methods: MC3T3-E1 cells were exposed to dexamethasone (Dex) with or without KRG and cell viability was measured by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Realtime polymerase chain reaction was performed to evaluate the apoptotic gene expression; osteogenic gene expression and alkaline phosphatase (ALP) activity were also measured. Western blotting was performed to evaluate the mitogen-activated protein kinase (MAPK) proteins. A GC-induced osteoporosis animal model was used for in vivo study. Results and conclusion: The MTT assay revealed that Korean Red Ginseng (KRG) prevents loss of cell viability caused by Dex-induced apoptosis in MC3T3E1 cells. Real-time polymerase chain reaction data showed that groups treated with both Dex and KRG exhibited lower mRNA levels of caspase-3 and -9, whereas the mRNA levels of Bcl2, IAPs, and XIAP increased. Moreover, groups treated with both Dex and KRG demonstrated increased mRNA levels of ALP, RUNX2, and bone morphogenic proteins as well as increased ALP activity in MC3T3-E1 cells, compared to cells treated with Dex only. In addition, KRG increased protein kinase B (AKT) phosphorylation and decreased c-Jun N-terminal kinase (JNK) phosphorylation. Moreover, microcomputed tomography analysis of the femurs showed that GC implantation caused trabecular bone loss. However, a significant reduction of bone loss was observed in the KRGtreated group. These results suggest that the molecular mechanism of KRG in the GC-induced apoptosis may lead to the development of therapeutic strategies to prevent and/or delay osteoporosis.

친환경적인 무기질계 주입재(NDS)의 내구성에 관한 연구

이혜진(Lee Hyejin),이종휘(Lee Jonghwi),정경식(Jung kyoungsik),천병식(Chun Byungsik) 한국지반환경공학회 2011 한국지반환경공학회논문집 Vol.12 No.7

현재 지반주입공법에 사용되는 약액으로는 물유리계가 주로 사용되고 있다. 그러나 물유리계를 사용한 약액주입공법은 현장적용에 있어서 내구성 저하, 강도 저하, 용탈 현상 등의 여러 가지 문제점을 가지는 바, 한시적인 차수용도로 사용될 수는 있으나 영구적인 지반보강 및 차수를 위해서는 본질적인 문제점을 가지고 있다. 본연구에서는 이러한 물유리계 지반주입재의 문제점을 해결할 수 있는 무기질계 지반주입재(NDS)의 환경영향평가 및 내구성을 규명하기 위하여 대표적인 물유리계 재료인 SGR 재료와 함께 일축압축강도시험, 어독성시험, 내구성시험, 침적 후 삼축투수시험을 실시하였다. 내구성시험 결과, 재령 28일 강도는 NDS가 SGR보다 1.5배 크게 나타났으며, 어독성시험 결과 SGR의 생존율은 50~70%, NDS는 100%로 나타났고 이는 NDS의 생존율이 SGR보다 더 높다는 것을 보여주었다. 따라서 시험결과를 종합해 볼 때 전반적으로 NDS가 SGR에 비해 내구성면에서 우수하며 친환경 적임을 확인할 수 있었다. Recently, the ground injection method using water glass as one of the components of the main resources and the products of the construction has some basic problems for permanent reinforcement of foundation and stopping leakage of water because it has some serious problems such as durability reduction, compression strength reduction and eluviation. This study was to evaluate the environmental impact and durability of the developed friendliness of Natural and Durable Stabilizer(NDS) of inorganic injection and Space Grouting Roket(SGR) with typical water glass type material. Two materials, NDS and SGR, were compared with each other by unconfined compressive strength test, fish poison test, durability test and triaxial permeability test. The results of the durability test indicated that the 28-day strength of the NDS was 1.5 times higher than that of the SGR. The fish poison test proved that the survival rate in the SGR and NDS is 50~70%, and 100%, respectively. Therefore, the NDS has higher survival rate than that of the existing SGR. The NDS will be considered by an environmentally friendly product and moreover it has a few problems for soil and groundwater pollution.

스테레오 비전을 이용한 장애물 회피 로봇

전성국(Sungkuk Chun),최현철(Hyunchul Choi),권혜진(Hyejin Kwon),이유성(Yusung Lee),박안진(Anjin Park),정기철(Keechul Jung) 한국멀티미디어학회 2007 한국멀티미디어학회 학술발표논문집 Vol.2007 No.1

자동화 사회로 진입하면서 인간에게 더욱더 편리한 생활을 제공하기 위해 로봇의 사용이 급증하고 있으며 , 최근 로봇에 대한 다양한 연구가 진행되고 있다. 특히 주변 상황에 대응하여 로봇이 이동하는 기술의 중요성이 강조되고 있으며 로봇의 주요 연구 분야 중에 하나이다. 본 논문에서는 스테레오 비전 시스템을 통하여 객체를 판별하고 그 객체를 펴해 이동하는 환경 적응형 로봇을 제안한다. 우리는 로봇의 이동에 의해 수시로 변하는 주변 환경을 스테레오 비전 시스템을 통해 파악하고 실시간 로봇에 전송한다. 객체를 판별하기 위한 깊이 정보 추출을 위하여 이미지 블록화를 통한 화소 단위 정합을 사용하고, 화소 단위 정합 시 환경 변화에 따른 오차를 줄이기 위하여 외곽선 추출 방법을 사용한다. 이를 통해 로봇의 주변 환경을 실시간 파악함으로써 환경 변화에 신속하게 대응하는 로봇을 구현한다.