http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
cDNA Library Construction of Aphis gossypii Using Gateway Cloning System
Hye-Ri Kwon,Jung-Kyu Kim,Na-Yeon Ko,Yu-Bin Jung,Chan-yeong Kang,Tae-Hee Ryu,Mi-Ja Seo,Hyoun-Sub Lim,Yong-Man Yu,Young-Nam Youn 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10
Aphis gossypii was widely distributed throughout the tropical, subtropical and temperate zone. The chemical control of A. gossypii is becoming problem because it was rapidly appeared resistance expression to chemicals. We will attempt to resolve the this problem using RNAi technique. Besides, RNAi technology can be helpful to study the target genes of A. gossypii. In this study we produce cDNA library construction using gateway cloning system for selecting target gene in order to control of A. gossypii using RNAi. As a result, the 100-400bp of insert size, which is appropriate for RNAi was confirmed. Most of insert gene is associated with A. gossypii, after that insert sequence was compared with DNA databases and EST databases using NCBI blast search. Consequentially, A. gossypii of cDNA library with the titer of 3.15x105 clones were completed. And we will perform the LR recombination to transfer cDNA library into TRV2 (tobacco rattle virus) vector with att site. Then, after performing transformation using Agrobacterium tumefaciens (GV 2260), we inoculated to cucumber with A. tumefaciens. An insecticidal effect or a repellent activity against A. gossypii by changing behavior in transgenic cucumber plants were conformed. Also, the selecting target gene in order to control A. gossypii using RNAi may be provided.
Jeon, Hye-Yeon,Kim, Na-Ri,Lee, Hye-Won,Choi, Hye-Jeong,Choung, Woo-Jae,Koo, Ye-Seul,Ko, Dam-Seul,Shim, Jae-Hoon American Chemical Society 2016 Journal of agricultural and food chemistry Vol.64 No.11
<P>A novel maltose (G2)-forming alpha-amylase from Lactobacillus plantarum subsp. plantarum ST-III was expressed in Escherichia coli and characterized. Analysis of conserved amino acid sequence alignments showed that L. plantarum maltose producing alpha-amylase (LpMA) belongs to glycoside hydrolase family 13. The recombinant enzyme (LpMA) was a novel G2 producing alpha-amylase. The properties of.purified LpMA were investigated following enzyme purification. LpMA exhibited optimal activity at 30 degrees C and pH 3.0. It produced only G2 from the hydrolysis of various substrates, including maltotriose (G3), maltopentaose (GS), maltosyl beta-cyclodextrin (G2-beta-CD), amylose, amylopectin, and starch. However, LpMA was unable to hydrolyze cyclodextrins. Reaction pattern analysis using 4-nitrophenyl-alpha-D-maltopentaoside (pNPGS) demonstrated that LpMA hydrolyzed pNPGS from the nonreducing end, indicating that LpMA is an exotype alpha-amylase. Kinetic analysis revealed that LpMA had the highest catalytic efficiency (k(cat)/K-m ratio) toward G2-beta-CD. Compared with beta-amylase, a well-known G2-producing enzyme, LpMA produced G2 more efficiently from liquefied corn starch due to its ability to hydrolyze G3.</P>
중학생의 모둠 자유탐구 활동에 대한 교사의 피드백 효과에 대한 연구
나혜리 ( Hye-ri Na ),심규철 ( Kew-cheol Shim ) 韓國生物敎育學會 2017 생물교육 Vol.45 No.2
The purpose of this study was to investigate the effects of the teacher feedback on the scientific inquiry ability of middle school students in the group open inquiry, and their cognition of open inquiry activities. Subjects were 160 seventh graders, who were examined about the quality of inquiry activity according to groups with and without teacher feedback. For the groups with teacher feedback, they were provided three times with science teacher`s feedback in the middle of carrying out the group open inquiry activity in the follow three steps; planning, performing and writing a re-port of inquiry. Middle school students with teacher feedback showed higher scientific inquiry ability than that of them without teacher feedback. In addition, students with the tea-cher feedback had positive cognition about the open inquiry activity, and perceived that the scientific inquiry ability of them was improved. However, students without the teacher feedback experienced difficulty of the open inquiry activity. Thus, a teacher should provide feedback to students for their effective open inquiry performance, and guide them about the method which students comes to a teacher for feedback.
( Na Young Yoon ),( Hye Young Wang ),( Minyoung Jung ),( Dong Hye Kim ),( Noo Ri Lee ),( Seong Jun Seo ),( Eunhee Choi ),( Hyeyoung Lee ),( Eung Ho Choi ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Background: Hereditary factors of atopic dermatitis(AD) have been emphasized recently. AD-related gene mutations vary significantly across ethnicities. Objectives: We tried to find mutations infilaggrin(FLG), serine protease inhibitor Kazal-type 5(SPINK5) and kallikrein 7(KLK7) genes from Korean AD patients, and developa reverse blot hybridization assay(REBA) to apply forAD-related genes. Methods: We divided AD subjects into moderate to severe AD and mild AD groups, and also divided the subjects into extrinsic and intrinsic AD groups. We performed the functional studies for skin barrier state, and checked on genemutations using the REBA. Results: The mutant type(MT) of KLK7 was significantly more frequent in AD subjects than in control and higher in the moderate to severe group compared to the mild group. The MT frequency was not different between the intrinsic and extrinsic AD groups. In the SPINK5 mutation, AD subjects more frequently had the mixed type of 603-49A>T(Glu335Val), the MT of 1188T>C(His396His) and 2475G>T(Glu825Asp) compared to control, but there was no difference between intrinsic and extrinsic AD. Conclusion: We found a correlation between the KLK7 mutation and AD, the mutations in1188T>C and 2475G>T, and 603-49A>T of SPINK5 and AD which have not been reported in Asians including Koreans. Above all things, we verified that the REBA can be applied to detect multiple barrier-related gene mutations in AD easily, simply, and accurately.
( Hye Lim Kim ),( Si Hyun Bae ),( Jung Hoon Cha ),( Na Ri Park ),( Jong Young Choi ),( Seung Kew Yoon ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1
Background: Mesenchymal-Epithelial transition (MET) in hepatic stem cells is important to multiple processes, including hepatogenic differentiation and liver development, regeneration. Early growth response (EGR) 1, transcription factor of early growth response gene family, is induced in the responses to a number of growth and differentiation factors. The aim of this study is to investigate that EGR1 acts as a key regulator of EMT/MET in the process of hepatogenic differentiation from bone marrow-derived mesenchymal stem cells (BM-MSCs). Methods: Pas staining and Urea production test were performed to verify the functionality of hepatogenic differentiation. To search for novel proteins in BM-MSCs before and after differentiation, protein/DNA array was performed. Immunohistochemistry were identified the enhanced expression of EGR1 and MET markers, accompanied by decreased expression of EMT markers. To determine the effect of EGR1, BMMSCs were infected by lenti-shEGR1. To determine the role of EGR1 in BM-MSCs which were infected by lenti-shEGR1, MET/EMT markers during the hepatogenic differentiation were identified by immunoblotting, immuno- fluorescence. Results: During differentiation proceeds, from day 1 to day 28, stem cells change in shape from spindle to polygon with larger size and tighter intercellular connections. Intracellular glycogen and urea production was significantly increased from day 1 to day 28. The mRNA expression of liver-specific genes was upregulated. In order to study key regulator in hepatogenic differentiation, we performed protein/DNA array. EGR1 in differentiated BM-MSCs were significantly increased during the culture compared to undifferentiated BM-MSCs. In contrast, downregulation of EGR1 by lenti-shRNA reduced hepatogenic differentiation and increased the expression of EMT related genes in associaton with the decreased expression of MET related gene. Conclusions: In this study, we identified novel factors in the process of hepatogenic differentiation through inducing the MET process. Our study suggests that EGR1 were expected to the “transcription factor therapy” as novel strategy in future clinical treatment.
Na, Hae‐,Ri,Kim, SangYun,Choi, Seong‐,Hye,Yang, Dong‐,Won,Bae, Hee‐,Joon,Kim, Jung‐,Eun,Park, Mee‐,Young,Shim, Yong‐,Soo,Kim, Byung‐,Kun,Kwon, Jaeȁ Blackwell Publishing Asia 2011 GERIATRICS & GERONTOLOGY INTERNATIONAL Vol.11 No.1
<P><B>Aim: </B> Donepezil has not been evaluated in Korean patients with Alzheimer's disease (AD) for up to 1 year. The objectives of this study were to evaluate the differential efficacy of donepezil in Korean AD patients with and without concomitant cerebrovascular lesions (CVL).</P><P><B>Methods: </B> This study was a 48‐week open‐label trial of donepezil in patients with probable AD of mild to moderate severity. CVL were evaluated through magnetic resonance imaging (MRI) findings within 3 months. Efficacy analyses were performed for cognitive, behavioral and functional outcome measures.</P><P><B>Results: </B> Concomitant CVL were documented in 35 (30.7%) of the patients on MRI. Seventy‐nine (69.3%) of the patients were considered not to have concomitant CVL. The mean Mini‐Mental State Examination scores of both patients with and without CVL showed improvement at each evaluation. However, there was no statistical difference in improvement between the groups.</P><P><B>Conclusion: </B> The presence of CVL should not deter clinicians from treating AD with donepezil. <B>Geriatr Gerontol Int 2011; 11: 90–97.</B></P>
( Hye-lim Kim ),( Jung Hoon Cha ),( Na Ri Park ),( Won Hee Hur ),( Si Hyun Bae ),( Jong Young Choi ),( Seung Kew Yoon ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1
Background: Epithelial-to-mesenchymal transition (EMT) drives mammary epithelial cells to de-differentiate into mammary stem cells which are mesenchymal-like. MET and the reverse process, epithelial mesenchymal transition (EMT), both occur in normal tissue, including gastrulating and regenerating tissue. MET in hepatic stem cells is important to multiple processes, including hepatic differentiation, liver development and regeneration. In this study, We investigated regulator of EMT/MET process in the hepatic differentiation. Methods: Based on the 2 step protocol, bone marrow-derived mesenchymal stem cells were cultured for hepatic differentiation. Pas staining and Urea production test were performed to verify the functionality of hepatic differentiation. To search for novel proteins in MSC before and after differentiation, protein/ membrane array was performed. EMT/ MET markers were identified during the hepatic differentiation of MSC by Western blot. Results: During differentiation proceeds, from day 1 to day 28, stem cells change in shape from spindle to polygon with larger in size and tighter intercellular connections. Intracellular glycogen and urea production was significantly increased from day 1 to day 28. The mRNA expression of liver-specific genes was up-regulated. In order to study key regulators in hepatic differentiation, we performed protein/membrane array. Novel factors of EGR1, EGR2 and WT1 were significantly in differentiated MSCs increased during the culture compared to undifferentiated MSCs. The induction of MET markers, E-cadherin and CK18 and the reduction of EMT markers, vimentin and N-cadherin, were exhibited during hepatic differentiation. Conclusions: We identified the novel factors of EGR and WT1 which were expected to play a role in hepatic differentiation. In the future, the acceleration of differentiation by novel factors and EMT/MET process should be studied further.
( Hye Lim Kim ),( Jung Hoon Cha ),( Na Ri Park ),( Won Hee Hur ),( Si Hyun Bae ),( Jong Young Choi ),( Seung Kew Yoon ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-
Background: Epithelial-to-mesenchymal transition drives mammary epithelial cells to de-differentiate into mammary stem cells which are mesenchymal-like. MET and the reverse process, epithelial mesenchymal transition (EMT), both occur in normal tissue, including gastrulating and regenerating tissue. MET in hepatic stem cells is important to multiple processes, including hepatic differentiation, liver development and regeneration. In this study, We investigated regulator of EMT/MET process in the hepatic differentiation. Methods: Based on the 2 step protocol, bone marrow-derived mesenchymal stem cells were cultured for hepatic differentiation. Pas staining and Urea production test were performed to verify the functionality of hepatic differentiation. To search for novel proteins in MSC before and after differentiation, protein/ membrane array was performed. EMT/ MET markers were identified during the hepatic differentiation of MSC by Western blot. Results: During differentiation proceeds, from day 1 to day 28, stem cells change in shape from spindle to polygon with larger in size and tighter intercellular connections. Intracellular glycogen and urea production was significantly increased from day 1 to day 28. The mRNA expression of liver-specific genes was up-regulated. In order to study key regulators in hepatic differentiation, we performed protein/membrane array. Novel factors of EGR1, EGR2 and WT1 were significantly in differentiated MSCs increased during the culture compared to undifferentiated MSCs. The induction of MET markers, E-cadherin and CK18 and the reduction of EMT markers, vimentin and N-cadherin, were exhibited during hepatic differentiation. Conclusions: We identified the novel factors of EGR and WT1 which were expected to play a role in hepatic differentiation. In the future, the acceleration of differentiation by novel factors and EMT/MET process should be studied further.