http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Hwang, Sue Yun,Kim, Seung Hoon,Hwang, Sung Hee,Cho, Chul Soo,Kim, Ho Youn The Korean Society for Integrative Biology 2001 Korean journal of biological sciences Vol.5 No.2
A key aspect of genomic research in the “post-genome era”is to associate sequence variations with heritable phenotypes. The most common variations in the human genome are single nucleotide polymorphisms (SNPs) that occur approximately once in every 500 to 1,000 bases. Although analyzing the phenotypic outcome of these SNPs is crucial to facilitate large-scale association studies of genetic diseases, detection of SNPs from an extended number of human DNA samples is often difficult, labor-intensive and time-consuming. Recent development in SNP detection methods using DNA microarrays and mass spectrophotometry has allowed automated high throughput analyses, but such equipments are not accessible to many scientists. In this study, we demonstrate that a simple PCR-based method using primers with a mismatched base at the 3'-end provides a fast and easy tool to identify known SNPs from human genomic DNA in a regular molecular biology laboratory. Results from this PCR amplification of specific alleles (PASA) analysis efficiently and accurately typed the Q576R polymorphism of human IL4 receptor from the genomic DNAs of 29 Koreans, including 9 samples whose genotype could not be discerned by the conventiona1 PCR-SSCP (single strand conformation polymorphism) method. Given the increasing attention to disease-associated polymorphisms in genomic research, this alternative technique will be very useful to identify SNPs in large-scale population studies.
Hwang, Sung Chul,Jhon, Deok-Young,Rhee, Sue Goo 아주대학교 의과학연구소 1996 아주의학 Vol.1 No.1
During the purification of phospholipase C (PLC)-rl which was over-expressed in HeLa cells transfected with a recombinant vaccinia virus carrying a complete cDNA sequence for PLC-γ₁ we noticed the presence of a heat-stable activator in crude cytosolic extract of HeLa cells which markedly stimulated phosphatidylinositol (PI) hydrolysis, when reconstituted with purified PLC-γ₁. Moreover, this putative factor was also found to be present in bovine brain cytosol. Subsequently, based on its ability to stimulate PI hydrolysis of PLC-γ₁ as an assay, this activation factor was purified to homogeneity from bovine brain cytosol. It was purified by heat treatment, trichloroacetic acid precipitation, and successive chromatographic steps on DEAE-5PW, phenyl-5PW, and heparin-5PW HPLC columns. The purified protein, as seen on SDS-PAGE, consisted of 4 or 5 closely spaced bands of apparent molecular weight between 48 and 62 kDa. However, on gel filtration chromatography on TSK-G3000SW HPLC, the estimated molecular weight was revealed to be approximately 350 kDa. The purified activator was identified as a microtubule-associated protein tau by electroelution from an SDS-polyacrylamide gel, partial peptide mapping by Staphylococcal V_(8) protease, and amino acid sequencing of cyanogen bromide cleaved peptides. The identity of the activator was re-confirmed by an immunoblotring using a specific anti-lau monoclonal antibody. In addition, reconstituting PLC-γ₁ with tau protein purified by an alternative method described elsewhere^ resulted in the same magnitude of activation. Tau protein mediated activation of PLC isozymes was calcium dependent. Isozyme specific activation of PLCs in 0.1% deoxycholate substrate appeared to be preferential toward PI hydrolysis. The magnitude of activation of PLC isozymes in PI substrate were PLC-γ₁ > PLC-r2 > PLC-δ₁ > PLC-β₁ in decreasing order. Approximately 200 nM concentration of tau-protein produced 15.5-, 5.4-, 4.2-, and 1.6-fold increase in activity of these enzymes, respectively, in the presence of 1 mM free calcium ion.
황철수,이상훈,Hwang, Chul-Sue,Lee, Sang-Hun 한국토지주택공사 토지주택연구원 2012 LHI journal of land, housing, and urban affairs Vol.3 No.1
The purpose of this research was to propose housing estate planning that applies 3D spatial information to DAS housing estate planning program and to enhance applicability of 3D spatial analysis. In addition, this research evaluated the applicability of 3D spatial information to site planning of Korea Land and Housing Corporation and developed models for the purpose of applying the spatial information efficiently. Moreover, we tested applicability of LiDAR that can allow 3D spatial information to be more efficient and accurate. The results from cross section analysis implies that LiDAR has higher usability than existing geographic information. Additionally, this study shaded light on the suggested system development model that can simulate location or height of an apartment house by spatially analyzing difference between before-and-after site development. 본 연구는 단지설계과정에서 3차원 공간정보 가운데 최근 각광을 받는 LiDAR 자료를 통합하여 정보의 정확성을 높이고 활용성을 확인하는데 목표를 둔 연구이다. 즉, 실제 활용되는 프로그램인 LH공사 업무 중 단지설계프로그램인 DAS를 연구의 대상으로 삼아 3차원 공간정보의 적용가능성을 알아보고 이를 효과적으로 적용시키기 위한 시뮬레이터를 개발하여 구현하였다. 이를 위해 사례연구지역을 선정하여 개발 전 후의 모습을 시뮬레이션하고 이 과정에서 개발 전 모습을 LiDAR 자료를 이용한 방법과 기존의 수치지도 이용 방법에 따른 정확성을 살펴보고, 개발 후 모습을 설계자료를 이용하여 구현하였다. 그 결과 횡단면분석을 통해 분석한 결과 LiDAR가 기존의 지리정보 데이터 보다 높은 정확성을 지니고 있음을 알 수 있었다. 또한 LiDAR 데이터를 활용하여 생성된 수치표고모델(DEM)을 등고선으로 추출하여 DAS프로그램에 적용하는 방안을 제시하였으며, 설계자료를 이용한 모델 생성 일반화식을 제시하였다. 3차원 공간정보를 활용한 시뮬레이터 개발은 3차원 지형모델을 이용한 3차원 공간분석, 3차원 공간정보를 바탕으로 한 의사결정 업무에 활용 가능할 것으로 예상된다.