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Gang Yu,Jin-Liang Liu,Li-Qin Xie,Xue-Liang Wang,Shi-Hong Zhang,Hong-Yu Pan 한국미생물학회 2012 The journal of microbiology Vol.50 No.6
The entomopathogenic fungus Verticillium lecanii is a wellknown biocontrol agent. V. lecanii produces subtilisin-like serine protease (Pr1), which is important in the biological control activity of some insect pests by degrading insect cuticles. In this study, a subtilisin-like serine protease gene VlPr1 was cloned from the fungus and the VlPr1 protein was expressed in Escherichia coli. The VlPr1 gene contains an open reading frame (ORF) interrupted by three short introns, and encodes a protein of 379 amino acids. Protein sequence analysis revealed high homology with subtilisin serine proteases. The molecular mass of the protease was 38 kDa, and the serine protease exhibited its maximal activity at 40°C and pH 9.0. Protease activity was also affected by Mg2+ and Ca2+ concentration. The protease showed inhibitory activity against several plant pathogens, especially towards Fusarium moniliforme.
한국과 중국 대학생의 빨간색 셔츠에 대한 시각적 감성 비교
반홍우 ( Hong Yu Pan ),최종명 ( Jong Myoung Choi ) 한국감성과학회 2012 감성과학 Vol.15 No.1
본 연구는 빨간색 셔츠의 시각적 감성을 비교 분석하여 소비자 감성을 반영한 셔츠의 색채기획에 도움을 주고자, 한국과 중국 대학생을 대상으로 온라인상에서 시각적 평가방법으로 7가지 톤의 빨간색 색지와 빨간색 셔츠에 대한 시각적 감성과 선호도를 평가하였다. 평가된 자료는 빈도 분석, 평균, 요인 분석, t-test를 실시하여 분석하였다. 빨간색 셔츠의 시각적 감성은 스포티, 로맨틱, 클래식 등 3개의 감성요인이 추출되었는데, 이 요인들은 톤, 성과 국적에 따라 유의한 차이가 나타났다. 중국 대학생은 한국 대학생에 비해 strong톤의 빨간색 셔츠가 더 스포티하고, dark톤이 더 클래식하다고 지각하였다. 또한 한국 대학생은 deep톤의 빨간색 셔츠를 로맨틱하다고 평가하였으나, 중국 대학생은 light톤과 pale톤의 셔츠를 로맨틱하다고 평가하였다. 한편, 7가지 톤의 빨간색 셔츠에 대한 선호도는 국적 및 성에 따라 유의한 차이를 보였다. 중국 대학생은 한국 대학생보다 dark톤과 pale톤의 빨간색 셔츠를 더 구매하고 싶어 하였으나, 한국 대학생은 strong톤의 셔츠를 더 선호하였다. 또한 남자 대학생이 dark와 dull톤의 빨간색 셔츠를 더 선호하는 반면, 여자 대학생은 light톤의 빨간색 셔츠를 더 선호하였다. The purpose of this study was to compare the visual sensibility of red shirts in university students of two countries, Korea and China in order to suggest the color planning for shirts. Seven tonal red shirts` visual sensibility and preferences were evaluated by the students of Korea and from China in the sense of sight. Evaluated data were analyzed by using frequency analysis, average, factor analysis and t-test. The visual sensibility of red shirts was classified into three factors: sporty, romantic and classic. Those factors of visual sensibility showed a significant difference according to nationality and gender. The Chinese students had a more sporty feel to the strong tone, and a more classic feel to the dark tone than the Korean students. The deep tone red shirts was reputed to be more romantic by the Korean students, but the light tone and the pale tone were considered more romantic by the Chinese students. Meanwhile, the effects of nationality and gender on seven tone of preferences for red shirts was significantly differed.
Correlation Between EGFR Mutations and Serum Tumor Markers in Lung Adenocarcinoma Patients
Pan, Jin-Bing,Hou, Yu-Hong,Zhang, Guo-Jun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2
Background: Mutations affecting the epidermal growth factor receptor (EGFR) are good predictors of clinical efficacy of EGFR tyrosine kinase inhibitors (TKI) in patients with non-small cell lung cancer. Serum carcinoembryonic antigen (CEA) levels are also regarded as predictive for the efficacy of EGFR-TKI and EGFR gene mutations. This study analyzed the association between EGFR gene mutations and clinical features, including serum tumor marker levels in lung adenocarcinomas patients. Patients and Methods: A total of 70 lung adenocarcinoma patients with complete clinical data and pathological specimens were investigated. EGFR gene mutations at exons 19 and 21 were assessed. Serum tumor markers were detected by protein chip-chemiluminescence at the corresponding time, and correlations were analyzed. Results: Mutations of the EGFR gene were detected in 27 of the 70 patients and the serum CEA and CA242 concentrations were found to be significantly associated with the incidence of EGFR gene mutations (P<0.05). The AUCs for CEA and CA242 were 0.724 (95% CI: 0.598~0.850, P<0.05) and 0.769 (95% CI: 0.523~0.800, P<0.05) respectively. Conclusions: Serum CEA and CA242 levels are associated with mutations of the EGFR gene in patients with lung adenocarcinomas.
Hongli Yu,Hao Wu,Yaozong Pan,Xiuyun Ge,Qian Zhang,Fagen Zhu,Baochang Cai 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.10
The present study aimed at investigating thealum-processing mechanism attenuating toxicity of AraceaePinellia ternata and Pinellia pedatisecta. Animalretroperitoneal inflammatory model in vivo andmacrophagocyte release inflammatory factor model in vitrowere used to detect the effect of alum processing onraphides and lectin. Scanning electron microscopy wasused to observe the change in raphides during processing;HPLC method was used to determine the correlation betweenthe dissolution and corrosion of raphides and ion inthe alum solution; 27Al-NMR technology was used to detectthe relationship between aluminum oxalate complexformation and the dissolved and corrosion of raphides. Thechange in protein peptide sequence of lectin during theprocessing of alum solution was determined by ShotgunLC–MS assay. Raphides induced severe rabbit conjunctivaledema and an intraperitoneal injection of lectin increasedPGE2 and protein in mice peritoneal exudate, whiledecreased after treatment with alum solution processing. During the processing raphides was dissolved and corroded,then its structure was damaged. Raphides was soaked inthe alum solution and significantly decreased the oxalatecontent, and the effect was related with Al3? in the alum. Al3? in the alum combined with C2O42- of raphides into astable complex compound promoted the dissolution ofcalcium oxalate. Raphides soaked in the alum made lectinproteins dissolve, whereas protein peptide sequence oflectin was changed and the protein structure was damaged. Conclusion: Alum solution could decrease the toxicity ofP. ternata (Thunb.) Breit. and P. pedatisecta Schott. Sinceit made a special crystal structure of raphides damage andthe protein of lectin dissolve. The structure of toxic substancessignificantly changed, which decreased the inflammatoryeffect.
( Hong Xie ),( Jia Hu ),( Huan Pan ),( Ya Xin Lou ),( Ping Lv ),( Ying Yu Chen ) 생화학분자생물학회 2014 BMB Reports Vol.47 No.2
FAM176A (family with sequence similarity 176 member A) is a novel molecule related to programmed cell death. A decreased expression of FAM176A has been found in several types of human tumors in including lung cancers. In the present study, we investigated the biological activities of FAM176A on the human non-small cell lung cancer cell line H1299 cells. We constructed a recombinant adenovirus 5-FAM176A vector (Ad5-FAM176A) and evaluated the expression and anti-tumor activities in vitro. Cell viability analysis revealed that the adenovirus-mediated increase of FAM176A inhibited the growth of the tumor cells in a dose- and time-dependent manner. This inhibitory effect was mediated by both autophagy and apoptosis that involved caspase activation. In addition, cell cycle analysis suggested that Ad5-FAM176A could induce cell cycle arrest at the G2/M phase, all of which suggested that adenovirus-mediated FAM176A gene transfer might present a new therapeutic approach for lung cancer treatment. [BMB Reports 2014; 47(2): 104-109]
Antimycobacterial Activity of Fusaric Acid from a Mangrove Endophyte and its Metal Complexes
Jia-Hui Pan,Yi Chen,Yu-Hong Huang,Yi-Wen Tao,Jun Wang,Yan Li,Yi Peng,Tao Dong,Xiao-Min Lai,Yong-Cheng Lin 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.7
Due to the increasing prevalence of multidrug-resistant Mycobacterium tuberculosis, there is an urgent need for new antituberculosis drugs that have novel mechanisms of action. As part of our ongoing search for antimycobacterial metabolites from mangrove endophytes, chemical analysis of the active extract of a strain of Fusarium sp. was performed, which led to the isolation of fusaric acid as the predominant constituent. A variety of metal complexes of fusaric acid were prepared. Antimycobacterial assays showed that Cadmium (II) and Copper (II) complexes exhibited potent inhibitory activity against the M. bovis BCG strain [minimum inhibitory concentration (MIC) = 4 μg/mL] and the M. tuberculosis H37Rv strain (MIC = 10 μg/mL), respectively. This is the first report of the antimycobacterial activity of the mangrove Fusarium metabolite and its coordinating metal complexes.
Chen, Jie,Pan, Qin-Shi,Hong, Wan-Dong,Pan, Jingye,Zhang, Wen-Hui,Xu, Gang,Wang, Yu-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13
Statistical methods to analyze and predict the related risk factors of nosocomial infection in lung cancer patients are various, but the results are inconsistent. A total of 609 patients with lung cancer were enrolled to allow factor comparison using Student's t-test or the Mann-Whitney test or the Chi-square test. Variables that were significantly related to the presence of nosocomial infection were selected as candidates for input into the final ANN model. The area under the receiver operating characteristic (ROC) curve (AUC) was used to evaluate the performance of the artificial neural network (ANN) model and logistic regression (LR) model. The prevalence of nosocomial infection from lung cancer in this entire study population was 20.1% (165/609), nosocomial infections occurring in sputum specimens (85.5%), followed by blood (6.73%), urine (6.0%) and pleural effusions (1.82%). It was shown that long term hospitalization (${\geq}22days$, P= 0.000), poor clinical stage (IIIb and IV stage, P=0.002), older age (${\geq}61days$ old, P=0.023), and use the hormones were linked to nosocomial infection and the ANN model consisted of these four factors. The artificial neural network model with variables consisting of age, clinical stage, time of hospitalization, and use of hormones should be useful for predicting nosocomial infection in lung cancer cases.