跨世紀韓國與中國山東省 經貿合作前景展望

范振洪,李炳鎭 평택대학교 1999 論文集 Vol.13 No.-

本文??述了跨世紀韓國與中國山동省經貿合作的前景問題. (1) 跨世紀韓國與中國山東省經貿合作的有利條件 制約要所 (2) 跨世紀韓國與中國山東省經貿合作的重点 ; (3) 跨世紀韓國與中國山東省經貿合作的對策.

An experimental model-based exploration of cytokines in ablative radiation-induced lung injury in vivo and in vitro.

Hong, Zhen-Yu,Song, Kwang Hyun,Yoon, Joo-Heon,Cho, Jaeho,Story, Michael D Springer International 2015 Lung Vol.193 No.3

<P>Stereotactic ablative radiotherapy is a newly emerging radiotherapy treatment method that, compared with conventionally fractionated radiation therapy (CFRT), allows an ablative dose of radiation to be delivered to a confined area around a tumor. The aim of the present study was to investigate the changes of various cytokines that may be involved in ablative radiation-induced lung injury in vitro and in vivo.</P>

Reference gene selection for RT-qPCR analysis in two invasive whiteflies after the acquisition of vectored or non-vectored viruses

Zhen-Hong Lv,Hui-Peng Pan,Wei Zhang,Tian-Bo Ding,Dong Chu 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

Custom reference gene selection is essential for reverse transcriptase-quantitative polymerase chain reaction(RT-qPCR) in different species of insects and various experiment conditions. In this study, 14 candidate referencegenes (HSP40, HSP20, HSP70, HSP90, v-ATPase, RPL29, EF-1, SDHA, Actin, PPIA, GAPDH, MyosinL,NADH, and γ-tubulin) were analyzed using five different programs including ΔCt method, BestKeeper, geNorm,NormFinder, and ReFinder to validate their use as reference genes in two invasive whiteflies, Bemisia tabaci Band Q, after acquiring the vectored virus, Tomato yellow leaf curl virus (TYLCV), or ingesting the non-vectoredvirus, Tomato spotted wilt virus (TSWV), respectively. The results showed that HSP40, v-ATPase, and EF-1 werethe most stable genes in B. tabaci B (B. tabaci B feeding on the healthy, TYLCV- and TSWV-infected tomatoplant), PPIA, SDHA, and RPL29 were the most stable genes in B. tabaci Q (B. tabaci Q feeding on the healthy,TYLCV- and TSWV-infected tomato plant). In addition, EF-1, RPL29, and HSP20 were the most stable referencegenes in B. tabaci B and Q. These findings provide the basis for future RT-qPCR-based studies on whitefly-virus interactions. Meanwhile,this report may set a precedent for reference gene selection in insects after the ingestion of non-vectored viruses.

A new high-order response surface method for structural reliability analysis

Hong-Shuang Li,Zhen-Zhou Lu,Hong-Wei Qiao 국제구조공학회 2010 Structural Engineering and Mechanics, An Int'l Jou Vol.34 No.6

In order to consider high-order effects on the actual limit state function, a new response surface method is proposed for structural reliability analysis by the use of high-order approximation concept in this study. Hermite polynomials are used to determine the highest orders of input random variables, and the sampling points for the determination of highest orders are located on Gaussian points of Gauss-Hermite integration. The cross terms between two random variables, only in case that their corresponding percent contributions to the total variation of limit state function are significant, will be added to the response surface function to improve the approximation accuracy. As a result, significant reduction in computational cost is achieved with this strategy. Due to the addition of cross terms, the additional sampling points, laid on two-dimensional Gaussian points off axis on the plane of two significant variables, are required to determine the coefficients of the approximated limit state function. All available sampling points are employed to construct the final response surface function. Then, Monte Carlo Simulation is carried out on the final approximation response surface function to estimate the failure probability. Due to the use of high order polynomial, the proposed method is more accurate than the traditional second-order or linear response surface method. It also provides much more efficient solutions than the available high-order response surface method with less loss in accuracy. The efficiency and the accuracy of the proposed method compared with those of various response surface methods available are illustrated by five numerical examples.

IN SITU PREPARATION AND INHIBITORY ACTIVITY OF HYDROXYAPATITE/SILVER NANOCOMPOSITE

HONG-ZHEN XIE,JIAN-DONG WANG,CHONG-XIAO LUO,JIN-KU LIU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2012 NANO Vol.7 No.6

The hydroxyapatite (HAP) assembled microsphere chains/silver (Ag) nanocomposites were prepared by a facile in situ preparation method under hydrothermal condition. The crystal structure of products was characterized by X-ray di®raction (XRD) and the morphologies of products were investigated by transmission electron microscopy (TEM). The nanocomposites own large surface area and can hydrogen-bond to other substances by the hydroxyl group on the surface. The nanocomposites have good structural stability under the electrostatic e®ect of the silver nanoparticles and strong adsorbability of the HAP microsphere chains. The two substances combine to form special nanocomposite spheres. Specially, the HAP/Ag nanocomposites have high inhibitory activity and can be applied in the environment and medicine ¯elds. The nanocomposite structures can save the consumption of Ag materials.

Development of a small animal model to simulate clinical stereotactic body radiotherapy-induced central and peripheral lung injuries

Hong, Zhen-Yu,Eun, Sung Ho,Park, Kwangwoo,Choi, Won Hoon,Lee, Jung Il,Lee, Eun-Jung,Lee, Ji Min,Story, Michael D.,Cho, Jaeho Oxford University Press 2014 JOURNAL OF RADIATION RESEARCH Vol.55 No.4

<P>Given the tremendous potential of stereotactic body radiotherapy (SBRT), investigations of the underlying radiobiology associated with SBRT-induced normal tissue injury are of paramount importance. This study was designed to develop an animal model that simulates centrally and peripherally located clinical SBRT-induced lung injuries. A 90-Gy irradiation dose was focally delivered to the central and peripheral areas of the left mouse lung with an image-guided small-animal irradiation system. At 1, 2 and 4 weeks after irradiation, micro-computed tomography (micro-CT) images of the lung were taken. Lung function measurements were performed with the Flexivent<SUP>®</SUP> system (SCIREQ<SUP>©</SUP>, Montreal, Canada). For the histopathological analysis, the lungs were fixed by perfusing with formalin, and paraffin sections were stained with hematoxylin and eosin and Masson's Trichrome. Gross inspection clearly indicated local lung injury confined to the central and peripheral areas of the left lung. Typical histopathological alterations corresponding to clinical manifestations were observed. The micro-CT analysis results appeared to correlate with the histopathological findings. Mouse lung tissue damping increased dramatically at central settings, compared with that at the control or peripheral settings. An animal model to simulate clinical SBRT-induced central and peripheral lung injuries was developed and validated with histopathological, radiological and functional analyses. This model increases our understanding of SBRT-induced central and peripheral lung injuries and will help to improve radiation therapy in the future.</P>

Efficient and Cost-Reduced Glucoamylase Fed-Batch Production with Alternative Carbon Sources

( Hong Zhen Luo ),( Han Liu ),( Zhenni He ),( Cong Zhou ),( Zhong Ping Shi ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.2

Glucoamylase is an important industrial enzyme. Glucoamylase production by industrial Aspergillus niger strain featured with two major problems: (i) empirical substrate feeding methods deteriorating the fermentation performance; and (ii) the high raw materials cost limiting the economics of the glucoamylase product with delegated specification. In this study, we first proposed a novel three-stage varied-rate substrate feeding strategy for efficient glucoamylase production in a 5 L bioreactor using the standard feeding medium, by comparing the changing patterns of the important physiological parameters such as DO, OUR, RQ, etc., when using different substrate feeding strategies. With this strategy, the glucoamylase activity and productivity reached higher levels of 11,000 U/ml and 84.6 U/ml/h, respectively. The performance enhancement in this case was beneficial from the following results: DO and OUR could be controlled at the higher levels (30%, 43.83 mmol/l/h), while RQ was maintained at a stable/lower level of 0.60 simultaneously throughout the fed-batch phase. Based on this three-stage varied-rate substrate feeding strategy, we further evaluated the economics of using alternative carbon sources, attempting to reduce the raw materials cost. The results revealed that cornstarch hydrolysate could be considered as the best carbon source to replace the standard and expensive feeding medium. In this case, the production cost of the glucoamylase with delegated specification (5,000 U/ml) could be saved by more than 61% while the product quality be ensured simultaneously. The proposed strategy showed application potential in improving the economics of industrial glucoamylase production.

A Functional SNP in the MDM2 Promoter Mediates E2F1 Affinity to Modulate Cyclin D1 Expression in Tumor Cell Proliferation

Yang, Zhen-Hai,Zhou, Chun-Lin,Zhu, Hong,Li, Jiu-Hong,He, Chun-Di Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Background: The MDM2 oncogene, a negative regulator of p53, has a functional polymorphism in the promoter region (SNP309) that is associated with multiple kinds of cancers including non-melanoma skin cancer. SNP309 has been shown to associate with accelerated tumor formation by increasing the affinity of the transcriptional activator Sp1. It remains unknown whether there are other factors involved in the regulation of MDM2 transcription through a trans-regulatory mechanism. Methods: In this study, SNP309 was verified to be associated with overexpression of MDM2 in tumor cells. Bioinformatics predicts that the T to G substitution at SNP309 generates a stronger E2F1 binding site, which was confirmed by ChIP and luciferase assays. Results: E2F1 knockdown downregulates the expression of MDM2, which confirms that E2F1 is a functional upstream regulator. Furthermore, tumor cells with the GG genotype exhibited a higher proliferation rate than TT, correlating with cyclin D1 expression. E2F1 depletion significantly inhibits the proliferation capacity and downregulates cyclin D1 expression, especially in GG genotype skin fibroblasts. Notably, E2F1 siRNA effects could be rescued by cyclin D1 overexpression. Conclusion: Taken together, a novel modulator E2F1 was identified as regulating MDM2 expression dependent on SNP309 and further mediates cyclin D1 expression and tumor cell proliferation. E2F1 might act as an important factor for SNP309 serving as a rate-limiting event in carcinogenesis.

A new high-order response surface method for structural reliability analysis

Li, Hong-Shuang,Lu, Zhen-Zhou,Qiao, Hong-Wei Techno-Press 2010 Structural Engineering and Mechanics, An Int'l Jou Vol.34 No.6

In order to consider high-order effects on the actual limit state function, a new response surface method is proposed for structural reliability analysis by the use of high-order approximation concept in this study. Hermite polynomials are used to determine the highest orders of input random variables, and the sampling points for the determination of highest orders are located on Gaussian points of Gauss-Hermite integration. The cross terms between two random variables, only in case that their corresponding percent contributions to the total variation of limit state function are significant, will be added to the response surface function to improve the approximation accuracy. As a result, significant reduction in computational cost is achieved with this strategy. Due to the addition of cross terms, the additional sampling points, laid on two-dimensional Gaussian points off axis on the plane of two significant variables, are required to determine the coefficients of the approximated limit state function. All available sampling points are employed to construct the final response surface function. Then, Monte Carlo Simulation is carried out on the final approximation response surface function to estimate the failure probability. Due to the use of high order polynomial, the proposed method is more accurate than the traditional second-order or linear response surface method. It also provides much more efficient solutions than the available high-order response surface method with less loss in accuracy. The efficiency and the accuracy of the proposed method compared with those of various response surface methods available are illustrated by five numerical examples.

중국 상고시대 예제(禮制)의 발생 -묘장 출토 입장기물(장기(葬器))을 중심으로-

단굉진 ( Hong Zhen Duan ) 중국사학회 2007 中國史硏究 Vol.51 No.-