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Functional mechanisms for diabetic nephropathy-associated genetic variants
Hong Xu,Chengxin Gong,Yonghu Xu,Yongfang Fan,Xingzi Liu,Chaopeng Xiong,Luling He,Changle Liu,Shenqiang Rao,Wen Xiao,Lu Ding,Lan Tang,Fangfang Hu,Mengqi Xiong,Mei Yang,Shangdong Liang 한국유전학회 2016 Genes & Genomics Vol.38 No.7
Diabetic nephropathy (DN) is one of the major complications of diabetes. A tremendous amount of genetic variations have been identified to be associated with DN. However, most of them only generate from statistical associations at the DNA level, generally without direct functional evidence regarding their association mechanisms underlying DN. Based on the publicly available datasets and resources, this study performed integrative analyses (expression quantitative trait loci analysis, differential gene expression analysis and functional prediction analysis) to detect the molecular functional mechanisms underlying the associations for DN. Among 150 selected (P\E-4) genetic associations that were archived in the public databases, two single nucleotide polymorphisms (SNPs) (rs3135377 and rs9469220) have been found to act as cis-effect regulators of the ‘‘identified’’ gene (HLADRA and HLA-DRB1). These eQTL genes have differential expression signals in the DN-associated cell groups. These SNPs were predicted as regulatory sites by utilizing online prediction tools. Our data suggest potential mechanistic links underlying the association between DN and two identified SNPs. These results could help us to have a deeper understanding of the functional relevance of genetic variants with susceptibility to DN, which is useful for pursuit of in-depth validation studies to dissect their involvements and molecular functional mechanisms in DN.
Dynamic model-based back-stepping control design for-trajectory tracking of seabed tracked vehicles
Hong Xiong,Yuxiang Chen,Yuxiao Li,Hong Zhu,Chunliang Yu,Jingguo Zhang 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.8
Tracked vehicles are widely used as seabed production tools to ensure a stable motion on soft sediments. However, the slippage resulted from the complex nonlinear trackterrain interaction while trajectory tracking causes problems for precisely predicting the motion. Accordingly, a “proper” motion control method is necessitated. This work proposes a novel dynamic modeling approach and motion control method for seabed tracked vehicles under nonholonomic constraints, with the inclusion of the effects of the bulldozing resistance, compaction resistance, water resistance, and the direction and velocity of the current. The backstepping control based on a model-based proportional-integral-derivative three degrees-offreedom method is applied in the controller, and its stability is proven by Lyapunov theory. The effectiveness and accuracy of the method in controlling seabed tracked vehicles are validated by simulation examples.
Lee, Hong-Gu,Yin, Jin-Long,Xu, Cheng-Xiong,Hong, Zhong-Shan,Lee, Zhe-Hu,Jin, Yong-Cheng,Choi, Chang-Weon,Lee, Do-Hyeung,Kim, Kyoung-Hoon,Choi, Yun-Jaie Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.12
This study was conducted to examine the effects of glucose, chromium picolinate (CrP), and vitamin C (Vit C) on lipid metabolism in Korean native steers fitted with indwelling catheters. A total of 12 Korean native steers were randomly allocated to the following treatments: 1) normal control diet, 2) same as 1) +250 g of glucose by intravenous (IV) infusion, 3) same as 2)+13.5 g CrP administered orally, and 4) same as 3)+2.52 g Vit C by IV infusion. Glucose, Vit C, and CrP treatments were administered for five days. At days 1 and 3, serum insulin was higher in treated animals than in those fed the control diet (p<0.05). Serum non-esterified fatty acid (NEFA) concentration in the steers on treatment 2), control+13.5 g CrP, was lower than those on other treatments at 90 min post-infusion on days 1 and 3 (p<0.05). The expression of peroxisome proliferator-activated receptor-${\gamma}$ (PPAR${\gamma}$)2, stearoyl-CoA desaturase-1 (SCD), fatty acid synthase (FAS), and glucose transporter type 4 (Glut 4) in the longissimus muscle of steers on treatment 2 was higher than those on other treatments. In conclusion, the results suggest that CrP is associated with the regulation of gene expression involved in adipogenesis.
Xiong, ZhongHua(웅중화),Hong, Chan Seok(홍찬석) 한국전시산업융합연구원 2020 한국과학예술융합학회 Vol.38 No.5
The Traffic safety signs are easy-to-understand, people-oriented, and safety first. However, these traits, which are believed to be consistent, have often been erroneous and even misleading in the applications of traffic safety signs because the fact that most traits are generated under the same standard of understanding to the exclusion of individual differences. By comparing the contents of traffic safety signs and different objects, the study reveals that these objects hold different understandings towards different signs and even the same sign. Actually, the existing safety signs are designed in accordance with the understanding of adults, and children’s lack of understanding towards the existing safety signs would eventually result in traffic accidents on them. The study probes into 10 independent sign samples and 2 groups of sign samples with the same elements for a comparison of understanding of kids and adults towards safety signs, in a multidisciplinary context, alongside an analysis of their understanding. By analyzing the degree of understanding, the factors and relationships impacting the understanding (gender, age, educational background, environment, overall selections of kids and adults, complexity of symbolic elements, and color) could be figured out. The main children in the study ranged from 7 to 12 years old, the adult was divided into 18 years old, and the largest was 50 years old, so the study results were for these two ranges. The results showed that children and adults have different understandings of safety signs, and the average understanding level of adults is higher than that of children.
Xiong, Ai Sheng,Yao, Quan-Hong,Peng, Ri-He,Li, Xian,Fan, Hui-Qin,Guo, Mei-Jin,Zhang, Si-Liang Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.3
Phytases catalyze the release of phosphate from phytic acid. Phytase-producing microorganisms were selected by culturing the soil extracts on agar plates containing phytic acid. Two hundred colonies that exhibited potential phytase activity were selected for further study. The colony showing the highest phytase activity was identified as Aspergillus niger and designated strain 113. The phytase gene from A. niger 113 (phyI1) was isolated, cloned, and characterized. The nucleotide and deduced amino acid sequence identity between phyI1 and phyA from NRRL3135 were 90% and 98%, respectively. The identity between phyI1 and phyA from SK-57 was 89% and 96%. A synthetic phytase gene, phyI1s, was synthesized by successive PCR and transformed into the yeast expression vector carrying a signal peptide that was designed and synthesized using P. pastoris biased codon. For the phytase expression and secretion, the construct was integrated into the genome of P. pastoris by homologous recombination. Over-expressing strains were selected and fermented. It was discovered that ~4.2 g phytase could be purified from one liter of culture fluid. The activity of the resulting phytase was 9.5 U/mg. Due to the heavy glycosylation, the expressed phytase varied in size (120, 95, 85, and 64 kDa), but could be deglycosylated to a homogeneous 64 kDa species. An enzymatic kinetics analysis showed that the phytase had two pH optima (pH 2.0 and pH 5.0) and an optimum temperature of $60^{\circ}C$.
Directed Evolution of Beta-galactosidase from Escherichia coli into Beta-glucuronidase
Xiong, Ai-Sheng,Peng, Ri-He,Zhuang, Jing,Liu, Jin-Ge,Xu, Fang,Cai, Bin,Guo, Zhao-Kui,Qiao, Yu-Shan,Chen, Jian-Min,Zhang, Zhen,Yao, Quan-Hong Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.3
In vitro directed evolution through DNA shuffling is a powerful molecular tool for creation of new biological phenotypes. E. coli $\beta$-galactosidase and $\beta$-glucuronidase are widely used, and their biological function, catalytic mechanism, and molecular structures are well characterized. We applied an in vitro directed evolution strategy through DNA shuffling and obtained five mutants named YG6764, YG6768, YG6769, YG6770 and YG6771 after two rounds of DNA shuffling and screening, which exhibited more $\beta$-glucuronidase activity than wild-type $\beta$-galactosidase. These variants had mutations at fourteen nucleic acid sites, resulting in changes in ten amino acids: S193N, T266A, Q267R, V411A, D448G, G466A, L527I, M543I, Q626R and Q951R. We expressed and purified those mutant proteins. Compared to the wild-type protein, five mutant proteins exhibited high $\beta$-glucuronidase activity. The comparison of molecular models of the mutated and wildtype enzymes revealed the relationship between protein function and structural modification.
Noise in ZnO nanowire field effect transistors.
Xiong, Hao D,Wang, Wenyong,Suehle, John S,Richter, Curt A,Hong, Woong-Ki,Lee, Takhee American Scientific Publishers 2009 Journal of Nanoscience and Nanotechnology Vol.9 No.2
<P>The noise power spectra in ZnO nanowire field effect transistors (FETs) were experimentally investigated and showed a classical 1/f dependence. A Hooge's constant of 5 x 10(-3) was estimated. This value is within the range reported for CMOS FETs with high-k dielectrics, supporting the concept that nanowires can be utilized for future beyond-CMOS electronic applications from the point of view of device noise properties. ZnO FETs measured in a dry O2 environment displayed elevated noise levels compared to in vacuum. At low temperature, random telegraph signals are observed in the drain current.</P>
Parecoxib: an Enhancer of Radiation Therapy for Colorectal Cancer
Xiong, Wei,Li, Wen-Hui,Jiang, Yong-Xin,Liu, Shan,Ai, Yi-Qin,Liu, Rong,Chang, Li,Zhang, Ming,Wang, Xiao-Li,Bai, Han,Wang, Hong,Zheng, Rui,Tan, Jing Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2
Background: To study the effect of parecoxib, a novel cyclooxygenase-2 selective inhibitor, on the radiation response of colorectal cancer (CRC) cells and its underlying mechanisms. Materials and Methods: Both in vitro colony formation and apoptosis assays as well as in vivo mouse xenograft experiments were used to explore the radiosensitizing effects of parecoxib in human HCT116 and HT29 CRC cells. Results: Parecoxib sensitized CRC cells to radiation in vitro with a sensitivity enhancement ratio of 1.32 for HCT116 cells and 1.15 for HT29 cells at a surviving fraction of 0.37. This effect was partially attributable to enhanced apoptosis induction by parecoxib combined with radiation, as illustrated using an in vitro apoptosis assays. Parecoxib augmented the tumor response of HCT116 xenografts to radiation, achieving growth delay more than 20 days and an enhancement factor of 1.53. In accordance with the in vitro results, parecoxib combined with radiation resulted in less proliferation and more apoptosis in tumors than radiation alone. Radiation monotherapy decreased microvessel density (MVD) and microvessel intensity (MVI), but increased the hypoxia level in xenografts. Parecoxib did not affect MVD, but it increased MVI and attenuated hypoxia. Conclusions: Parecoxib can effectively enhance radiation sensitivity in CRC cells through direct effects on tumor cells and indirect effects on tumor vasculature.